safety

PPRS ARGs VFs PGs Plasmid Prophage IS elements

ARGs	VFs	PGs	PPRS
13	10	4	16.88

PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).

Comprehensive Antibiotic Resistance Database (CARD)

Query ID	Begin	End	ARO name	ARO accession	CARD name	Identity	Coverage	Accession no.
AOHCOEIK_1	2735280	2736134	aadK	ARO:3002627	aadK	100.00	100.00	AL009126.1
AOHCOEIK_1	2716571	2717773	blt	ARO:3003006	blt	100.00	100.00	L32599.1
AOHCOEIK_1	2494253	2495422	bmr	ARO:3003007	bmr	99.74	100.00	M33768.1
AOHCOEIK_1	1376453	1376770	ykkD	ARO:3003064	ykkD	100.00	100.00	AL009126.1
AOHCOEIK_1	1376115	1376453	ykkC	ARO:3003063	ykkC	100.00	100.00	AL009126.1
AOHCOEIK_1	916376	918946	Bacillus subtilis mprF	ARO:3003324	Bsub_mprF	100.00	100.00	AL009126.3
AOHCOEIK_1	604336	605979	vmlR	ARO:3004476	vmlR	100.00	100.00	NC_000964.3
AOHCOEIK_1	338755	339348	tmrB	ARO:3003059	tmrB	100.00	100.00	AL009126.3
AOHCOEIK_1	288252	289685	lmrB	ARO:3002813	lmrB	100.00	100.00	JYFL01000006.1
AOHCOEIK_1	275437	276357	mphK	ARO:3004541	mphK	100.00	100.00	CP010314.1

RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)

ResFinder

Query ID	Begin	End	Resistance gene	Phenotype	Identity	Coverage	Accession no.
AOHCOEIK_1	2735280	2736134	aadK	Streptomycin	100.00	100.00	M26879
AOHCOEIK_1	275437	276357	mph(K)	Spiramycin, Telithromycin	100.00	100.00	NC_000964
AOHCOEIK_1	4186508	4187884	tet(L)	Doxycycline, Tetracycline, Doxycycline, Tetracycline, Minocycline, Tigecycline	100.00	100.00	X08034

ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)

AMRFinderPlus

Query ID	Begin	End	Gene symbol	Sequence name	Method	Identity	Coverage	Accession no.
AOHCOEIK_1	2735283	2736134	aadK	aminoglycoside 6-adenylyltransferase AadK	EXACTX	100.00	100.00	WP_003229862.1
AOHCOEIK_1	275437	276354	mphK	macrolide 2'-phosphotransferase MphK	EXACTX	100.00	100.00	WP_003246254.1
AOHCOEIK_1	4183990	4184508	satA	streptothricin N-acetyltransferase SatA	EXACTX	100.00	100.00	WP_003242546.1
AOHCOEIK_1	4186511	4187884	tet(L)	tetracycline efflux MFS transporter Tet(L)	EXACTX	100.00	100.00	WP_003242953.1
AOHCOEIK_1	604339	605979	vmlR	ABC-F type ribosomal protection protein VmlR	EXACTX	100.00	100.00	WP_003234144.1
AOHCOEIK_1	2050933	2053527	rphC	rifamycin-inactivating phosphotransferase RphC	BLASTX	81.15	99.88	WP_087347987.1

AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)

Virulence Factor Database (VFDB)

Query ID	qstart	qend	Gene name	VF name	VF category	Identity	Coverage	Accession no.
AOHCOEIK_1	3697419	3698561	capA	Capsule	Immune modulation	100.00	100.00	NP_391469
AOHCOEIK_1	3699044	3700225	capB	Capsule	Immune modulation	100.00	100.00	NP_391471
AOHCOEIK_1	3698580	3699029	capC	Capsule	Immune modulation	100.00	100.00	NP_391470
AOHCOEIK_1	2004275	2006038	dep/capD	Capsule	Immune modulation	100.00	100.00	NP_389723
AOHCOEIK_1	3291109	3291894	dhbA	Bacillibactin	Nutritional/Metabolic factor	100.00	100.00	NP_391080
AOHCOEIK_1	3287273	3288211	dhbB	Bacillibactin	Nutritional/Metabolic factor	100.00	100.00	NP_391077
AOHCOEIK_1	3289887	3291083	dhbC	Bacillibactin	Nutritional/Metabolic factor	100.00	100.00	NP_391079
AOHCOEIK_1	3288239	3289858	dhbE	Bacillibactin	Nutritional/Metabolic factor	100.00	100.00	NP_391078
AOHCOEIK_1	3280117	3287253	dhbF	Bacillibactin	Nutritional/Metabolic factor	100.00	100.00	NP_391076
AOHCOEIK_1	2294901	2295542	hlyIII	Hemolysin III	Exotoxin	100.00	100.00	NP_390062

BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)

VirulenceFinder

Query ID	Begin	End	Database	Virulence factor	Protein function	Identity	Coverage	Accession no.
No hit found

VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)

PHI-base

Query ID	qstart	qend	Gene name	e-value	Function	Identity	Coverage	Gene ID
AOHCOEIK_1	132473	133609	tufA	0.0	elongation factor Tu	84.96	95.69	AHM69299
AOHCOEIK_1	18665	19534	pdxS	3.13e-149	encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS	83.79	98.31	ACE61268
AOHCOEIK_1	1689717	1690493	CodY	3.42e-124	nutrient-responsive regulator	81.08	100.00	ACJ80679
AOHCOEIK_1	3545832	3546401	ClpP	2.53e-98	part of proteolytic complex	81.05	97.44	BAB94595

BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)

PlasmidFinder

Query ID	Begin	End	Plasmid	Query / Template	Identity	Accession no.
No hit found

PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)

PlasmidHunter

Query ID	Prediction	Chromosome	Plasmid
AOHCOEIK_1	0.0	1.0	0.0

PlasmidHunter v 1.4.5 (Database: May. 2024 release)

Phigaro

Query ID	Begin	End	Transposable	Taxonomy	pVOGs
AOHCOEIK_1	1320168	1346276	False	Myoviridae	VOG4602, VOG9667, VOG3490, VOG0189, VOG4861, VOG5447, VOG0796, VOG10227, VOG4773, VOG4685, VOG0749, VOG3890, VOG3891, VOG3892, VOG6163, VOG4865, VOG4885, VOG3894, VOG4550, VOG4691, VOG4862, VOG7718, VOG4705
AOHCOEIK_1	2666643	2696887	False	Myoviridae	VOG7718, VOG4862, VOG4691, VOG4550, VOG3894, VOG4885, VOG4865, VOG6163, VOG3892, VOG3891, VOG3890, VOG10055, VOG4870, VOG4589, VOG0749, VOG4685, VOG4688, VOG4773, VOG10227, VOG0796, VOG4673, VOG9587, VOG4693, VOG0189, VOG11060, VOG1309, VOG4600

Phigaro v2.4.0 (Database: Jan. 2024 release)