safety

PPRS ARGs VFs PGs Plasmid Prophage IS elements

ARGs	VFs	PGs	PPRS
14	10	4	17.66

PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).

Comprehensive Antibiotic Resistance Database (CARD)

Query ID	Begin	End	ARO name	ARO accession	CARD name	Identity	Coverage	Accession no.
EENNMKAM_1	2707092	2707946	aadK	ARO:3002627	aadK	100.00	100.00	AL009126.1
EENNMKAM_1	2688383	2689585	blt	ARO:3003006	blt	100.00	100.00	L32599.1
EENNMKAM_1	2466075	2467244	bmr	ARO:3003007	bmr	99.74	100.00	M33768.1
EENNMKAM_1	1348334	1348651	ykkD	ARO:3003064	ykkD	100.00	100.00	AL009126.1
EENNMKAM_1	1347996	1348334	ykkC	ARO:3003063	ykkC	100.00	100.00	AL009126.1
EENNMKAM_1	888270	890840	Bacillus subtilis mprF	ARO:3003324	Bsub_mprF	100.00	100.00	AL009126.3
EENNMKAM_1	576234	577877	vmlR	ARO:3004476	vmlR	100.00	100.00	NC_000964.3
EENNMKAM_1	310655	311248	tmrB	ARO:3003059	tmrB	100.00	100.00	AL009126.3
EENNMKAM_1	298212	299006	APH(3')-IIIa	ARO:3002647	APH(3')-IIIa	100.00	100.00	CP004067.1
EENNMKAM_1	258442	259875	lmrB	ARO:3002813	lmrB	100.00	100.00	JYFL01000006.1
EENNMKAM_1	245627	246547	mphK	ARO:3004541	mphK	100.00	100.00	CP010314.1

RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)

ResFinder

Query ID	Begin	End	Resistance gene	Phenotype	Identity	Coverage	Accession no.
EENNMKAM_1	2707092	2707946	aadK	Streptomycin	100.00	100.00	M26879
EENNMKAM_1	298212	299006	aph(3')-III	Kanamycin, Amikacin, Neomycin, Butirosin, Isepamicin, Lividomycin, Paromomycin, Ribostamycin	100.00	100.00	M26832
EENNMKAM_1	245627	246547	mph(K)	Spiramycin, Telithromycin	100.00	100.00	NC_000964
EENNMKAM_1	4159055	4160431	tet(L)	Doxycycline, Tetracycline, Doxycycline, Tetracycline, Minocycline, Tigecycline	100.00	100.00	X08034

ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)

AMRFinderPlus

Query ID	Begin	End	Gene symbol	Sequence name	Method	Identity	Coverage	Accession no.
EENNMKAM_1	2707095	2707946	aadK	aminoglycoside 6-adenylyltransferase AadK	EXACTX	100.00	100.00	WP_003229862.1
EENNMKAM_1	298215	299006	aph(3')-IIIa	aminoglycoside O-phosphotransferase APH(3')-IIIa	EXACTX	100.00	100.00	WP_001096887.1
EENNMKAM_1	245627	246544	mphK	macrolide 2'-phosphotransferase MphK	EXACTX	100.00	100.00	WP_003246254.1
EENNMKAM_1	4156537	4157055	satA	streptothricin N-acetyltransferase SatA	EXACTX	100.00	100.00	WP_003242546.1
EENNMKAM_1	4159058	4160431	tet(L)	tetracycline efflux MFS transporter Tet(L)	EXACTX	100.00	100.00	WP_003242953.1
EENNMKAM_1	576237	577877	vmlR	ABC-F type ribosomal protection protein VmlR	EXACTX	100.00	100.00	WP_003234144.1
EENNMKAM_1	2022793	2025387	rphC	rifamycin-inactivating phosphotransferase RphC	BLASTX	81.15	99.88	WP_087347987.1

AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)

Virulence Factor Database (VFDB)

Query ID	qstart	qend	Gene name	VF name	VF category	Identity	Coverage	Accession no.
EENNMKAM_1	3669198	3670340	capA	Capsule	Immune modulation	100.00	100.00	NP_391469
EENNMKAM_1	3670823	3672004	capB	Capsule	Immune modulation	100.00	100.00	NP_391471
EENNMKAM_1	3670359	3670808	capC	Capsule	Immune modulation	100.00	100.00	NP_391470
EENNMKAM_1	1976138	1977901	dep/capD	Capsule	Immune modulation	100.00	100.00	NP_389723
EENNMKAM_1	3262905	3263690	dhbA	Bacillibactin	Nutritional/Metabolic factor	100.00	100.00	NP_391080
EENNMKAM_1	3259069	3260007	dhbB	Bacillibactin	Nutritional/Metabolic factor	100.00	100.00	NP_391077
EENNMKAM_1	3261683	3262879	dhbC	Bacillibactin	Nutritional/Metabolic factor	100.00	100.00	NP_391079
EENNMKAM_1	3260035	3261654	dhbE	Bacillibactin	Nutritional/Metabolic factor	100.00	100.00	NP_391078
EENNMKAM_1	3251913	3259049	dhbF	Bacillibactin	Nutritional/Metabolic factor	100.00	100.00	NP_391076
EENNMKAM_1	2266723	2267364	hlyIII	Hemolysin III	Exotoxin	100.00	100.00	NP_390062

BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)

VirulenceFinder

Query ID	Begin	End	Database	Virulence factor	Protein function	Identity	Coverage	Accession no.
No hit found

VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)

PHI-base

Query ID	qstart	qend	Gene name	e-value	Function	Identity	Coverage	Gene ID
EENNMKAM_1	102675	103811	tufA	0.0	elongation factor Tu	84.96	95.69	AHM69299
EENNMKAM_1	4206051	4206920	pdxS	3.13e-149	encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS	83.79	98.31	ACE61268
EENNMKAM_1	1661591	1662367	CodY	3.42e-124	nutrient-responsive regulator	81.08	100.00	ACJ80679
EENNMKAM_1	3517624	3518193	ClpP	2.54e-98	part of proteolytic complex	81.05	97.44	BAB94595

BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)

PlasmidFinder

Query ID	Begin	End	Plasmid	Query / Template	Identity	Accession no.
No hit found

PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)

PlasmidHunter

Query ID	Prediction	Chromosome	Plasmid
EENNMKAM_1	0.0	1.0	0.0

PlasmidHunter v 1.4.5 (Database: May. 2024 release)

Phigaro

Query ID	Begin	End	Transposable	Taxonomy	pVOGs
EENNMKAM_1	1292049	1318157	False	Myoviridae	VOG4602, VOG9667, VOG3490, VOG0189, VOG4861, VOG5447, VOG0796, VOG10227, VOG4773, VOG4685, VOG0749, VOG3890, VOG3891, VOG3892, VOG6163, VOG4865, VOG4885, VOG3894, VOG4550, VOG4691, VOG4862, VOG7718, VOG4705
EENNMKAM_1	2638457	2668701	False	Myoviridae	VOG7718, VOG4862, VOG4691, VOG4550, VOG3894, VOG4885, VOG4865, VOG6163, VOG3892, VOG3891, VOG3890, VOG10055, VOG4870, VOG4589, VOG0749, VOG4685, VOG4688, VOG4773, VOG10227, VOG0796, VOG4673, VOG9587, VOG4693, VOG0189, VOG11060, VOG1309, VOG4600

Phigaro v2.4.0 (Database: Jan. 2024 release)