Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 0 | 27 | 33 | 42.64 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| DIBLOPNL_48 | 89 | 635 | aadA1 | Spectinomycin, Streptomycin | 100.00 | 69.07 | JQ414041 |
| DIBLOPNL_53 | 1 | 519 | aph(3')-Ia | Neomycin, Kanamycin, Lividomycin, Paromomycin, Ribostamycin, Kanamycin, Neomycin, Kanamycin, Unknown Aminoglycoside | 99.81 | 63.60 | V00359 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| DIBLOPNL_48 | 86 | 634 | aadA1 | ANT(3'')-Ia family aminoglycoside nucleotidyltransferase AadA1 | PARTIAL_CONTIG_ENDX | 100.00 | 69.85 | WP_001206315.1 |
| DIBLOPNL_53 | 3 | 518 | aph(3')-Ia | aminoglycoside O-phosphotransferase APH(3')-Ia | PARTIAL_CONTIG_ENDX | 100.00 | 63.47 | WP_000018329.1 |
| DIBLOPNL_40 | 453 | 920 | erm(C) | 23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(C) | PARTIAL_CONTIG_ENDX | 91.67 | 63.11 | WP_007410443.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| DIBLOPNL_18 | 20378 | 20971 | STER_RS06030 | 0.0 | Capsule | Immune modulation | 100.00 | 100.00 | WP_011226149 |
| DIBLOPNL_3 | 154370 | 155086 | STER_RS07085 | 0.0 | Capsule | Immune modulation | 100.00 | 100.00 | WP_011681442 |
| DIBLOPNL_3 | 155087 | 156055 | STER_RS07090 | 0.0 | Capsule | Immune modulation | 100.00 | 100.00 | WP_011681443 |
| DIBLOPNL_3 | 145570 | 147315 | STER_RS07045 | 0.0 | Capsule | Immune modulation | 99.94 | 100.00 | WP_011681434 |
| DIBLOPNL_3 | 147317 | 149017 | STER_RS07050 | 0.0 | Capsule | Immune modulation | 99.94 | 100.00 | WP_041827753 |
| DIBLOPNL_3 | 157046 | 158305 | STER_RS07100 | 0.0 | Capsule | Immune modulation | 99.92 | 100.00 | WP_011681445 |
| DIBLOPNL_3 | 151981 | 153129 | STER_RS07070 | 0.0 | Capsule | Immune modulation | 99.91 | 100.00 | WP_011681439 |
| DIBLOPNL_18 | 19509 | 20378 | rfbA | 0.0 | Capsule | Immune modulation | 99.89 | 100.00 | WP_011681286 |
| DIBLOPNL_3 | 151043 | 151984 | STER_RS07065 | 0.0 | Capsule | Immune modulation | 99.89 | 100.00 | WP_011681438 |
| DIBLOPNL_3 | 153248 | 154024 | STER_RS07075 | 0.0 | Capsule | Immune modulation | 99.87 | 100.00 | WP_011681440 |
| DIBLOPNL_3 | 149035 | 150237 | STER_RS07055 | 0.0 | Capsule | Immune modulation | 99.83 | 100.00 | WP_011681436 |
| DIBLOPNL_18 | 20378 | 20971 | STR_RS05880 | 0.0 | Capsule | Immune modulation | 99.83 | 100.00 | WP_011226149 |
| DIBLOPNL_3 | 147317 | 149017 | STR_RS06890 | 0.0 | Capsule | Immune modulation | 99.77 | 100.00 | WP_041827077 |
| DIBLOPNL_18 | 20378 | 20971 | STU_RS15260 | 0.0 | Capsule | Immune modulation | 99.66 | 100.00 | WP_011226149 |
| DIBLOPNL_3 | 150237 | 151043 | STER_RS07060 | 0.0 | Capsule | Immune modulation | 99.63 | 100.00 | WP_011681437 |
| DIBLOPNL_3 | 156073 | 157049 | STER_RS07095 | 0.0 | Capsule | Immune modulation | 99.59 | 99.90 | WP_011681444 |
| DIBLOPNL_2 | 178115 | 179773 | fbp54 | 0.0 | FBPs | Adherence | 99.58 | 100.00 | WP_011227179 |
| DIBLOPNL_3 | 149035 | 150237 | STR_RS06895 | 0.0 | Capsule | Immune modulation | 99.58 | 100.00 | WP_011227428 |
| DIBLOPNL_14 | 20923 | 21762 | cbpD | 0.0 | Autolysin | Exoenzyme | 99.52 | 100.00 | WP_011226733 |
| DIBLOPNL_3 | 151043 | 151984 | STR_RS06905 | 0.0 | Capsule | Immune modulation | 99.36 | 100.00 | WP_011227430 |
| DIBLOPNL_3 | 151981 | 153129 | STR_RS06910 | 0.0 | Capsule | Immune modulation | 99.30 | 100.00 | WP_011227431 |
| DIBLOPNL_3 | 150237 | 151043 | STR_RS06900 | 0.0 | Capsule | Immune modulation | 99.13 | 100.00 | WP_011227429 |
| DIBLOPNL_2 | 68158 | 68889 | STER_RS05315 | 0.0 | Capsule | Immune modulation | 98.63 | 100.00 | WP_011681176 |
| DIBLOPNL_2 | 1379 | 2172 | cshA | 0.0 | Cell surface hydrophobicity proteins | Adherence | 98.36 | 100.25 | - |
| DIBLOPNL_3 | 145570 | 147315 | STR_RS06885 | 0.0 | Capsule | Immune modulation | 98.28 | 100.00 | WP_011227426 |
| DIBLOPNL_2 | 68158 | 68889 | STR_RS05260 | 0.0 | Capsule | Immune modulation | 98.22 | 100.00 | WP_011227248 |
| DIBLOPNL_2 | 68158 | 68889 | STU_RS14645 | 0.0 | Capsule | Immune modulation | 98.09 | 100.00 | WP_011226048 |
| DIBLOPNL_2 | 69600 | 70340 | STER_RS05305 | 0.0 | Capsule | Immune modulation | 97.98 | 100.00 | WP_011681174 |
| DIBLOPNL_2 | 66697 | 68157 | STER_RS05320 | 0.0 | Capsule | Immune modulation | 97.95 | 100.00 | WP_011681177 |
| DIBLOPNL_2 | 69600 | 70340 | STU_RS14635 | 0.0 | Capsule | Immune modulation | 97.84 | 100.00 | WP_011226046 |
| DIBLOPNL_15 | 24901 | 25662 | srtA | 0.0 | Sortase A | Exoenzyme | 97.64 | 100.00 | - |
| DIBLOPNL_3 | 158424 | 159275 | rfbD | 0.0 | Capsule | Immune modulation | 97.42 | 100.00 | WP_014621820 |
| DIBLOPNL_2 | 69600 | 70335 | STR_RS05250 | 0.0 | Capsule | Immune modulation | 97.42 | 98.13 | WP_011227246 |
| DIBLOPNL_2 | 66697 | 68157 | STU_RS14650 | 0.0 | Capsule | Immune modulation | 96.92 | 100.21 | WP_011226049 |
| DIBLOPNL_2 | 68898 | 69590 | STR_RS05255 | 0.0 | Capsule | Immune modulation | 96.83 | 100.00 | WP_011227247 |
| DIBLOPNL_2 | 68898 | 69590 | STU_RS14640 | 0.0 | Capsule | Immune modulation | 96.83 | 100.00 | WP_011226047 |
| DIBLOPNL_2 | 66697 | 68157 | STR_RS05265 | 0.0 | Capsule | Immune modulation | 95.96 | 100.21 | WP_041827043 |
| DIBLOPNL_2 | 70397 | 71762 | STER_RS05300 | 0.0 | Capsule | Immune modulation | 94.66 | 99.85 | WP_011681173 |
| DIBLOPNL_2 | 70397 | 71764 | STU_RS14630 | 0.0 | Capsule | Immune modulation | 94.08 | 100.00 | WP_011226045 |
| DIBLOPNL_2 | 68898 | 69590 | STER_RS05310 | 0.0 | Capsule | Immune modulation | 91.78 | 100.00 | WP_011681175 |
| DIBLOPNL_1 | 121294 | 122597 | eno | 0.0 | Streptococcal enolase | Exoenzyme | 88.10 | 99.46 | WP_000022832 |
| DIBLOPNL_21 | 14446 | 15456 | plr/gapA | 0.0 | Streptococcal plasmin receptor/GAPDH | Adherence | 86.54 | 99.41 | WP_002986042 |
| DIBLOPNL_3 | 150237 | 151043 | SGO_RS05020 | 0.0 | Capsule | Immune modulation | 83.27 | 100.00 | WP_012000440 |
| DIBLOPNL_10 | 6304 | 7587 | tig/ropA | 0.0 | Trigger factor | Stress survival | 80.65 | 99.77 | WP_000107753 |
| DIBLOPNL_18 | 21400 | 22446 | rfbB | 0.0 | Capsule | Immune modulation | 80.61 | 100.00 | WP_009659423 |
| DIBLOPNL_3 | 149500 | 150237 | SGO_RS05025 | 0.0 | Capsule | Immune modulation | 84.96 | 60.29 | WP_012000441 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| DIBLOPNL_15 | 22214 | 21231 | ldh(Sp_1220) | 0.0 | L-lactate dehydrogenase | 94.82 | 100.00 | AAK75326 |
| DIBLOPNL_1 | 14551 | 13478 | MntE | 0.0 | manganese homeostasis | 94.41 | 99.72 | ABF32167 |
| DIBLOPNL_4 | 15598 | 13295 | pfl2(Sp_0459) | 0.0 | formate acetyltransferase | 92.97 | 99.22 | AAK74619 |
| DIBLOPNL_1 | 204842 | 203874 | spr0084 | 0.0 | pathogenitcity related | 92.57 | 98.48 | AAK98888 |
| DIBLOPNL_3 | 50727 | 52208 | SPD_0310 | 0.0 | UPF0371 protein SPD_0310 | 92.11 | 100.00 | ABJ54480 |
| DIBLOPNL_17 | 11363 | 9117 | metE | 0.0 | methionine biosynthesis enzymes | 91.99 | 100.00 | AAK74738 |
| DIBLOPNL_14 | 40554 | 41552 | recA | 0.0 | DNA recombination and repair | 91.29 | 85.82 | AAL00560 |
| DIBLOPNL_14 | 4441 | 5412 | prsA2 | 0.0 | ribose-phosphate pyrophosphokinase | 90.12 | 100.00 | ABF36968 |
| DIBLOPNL_4 | 129611 | 130762 | potA | 0.0 | ABC transporter ATP-binding protein-spermidine/putrescine transport | 89.32 | 99.74 | AAL00050 |
| DIBLOPNL_3 | 172314 | 170326 | uvrB | 0.0 | excinuclease ABC, B subunit | 88.84 | 100.00 | AE014259_2 |
| DIBLOPNL_15 | 38413 | 40143 | ptsI | 0.0 | phosphoenolpyruvate-protein phosphotransferase | 88.04 | 100.00 | AAK34199 |
| DIBLOPNL_8 | 27273 | 28751 | IMPDH | 0.0 | inosine-5'-monophosphate dehydrogenase | 87.63 | 100.00 | ADE32434 |
| DIBLOPNL_1 | 122597 | 121296 | Eno | 0.0 | enolase | 86.21 | 99.77 | ABP90468 |
| DIBLOPNL_8 | 42462 | 44354 | gidA | 0.0 | tRNA uridine 5-carboxymethylaminomethyl modification enzyme | 85.89 | 98.90 | ABP91129 |
| DIBLOPNL_17 | 6193 | 7902 | PGM | 0.0 | phosphoglucomutase | 84.06 | 99.65 | ACH87041 |
| DIBLOPNL_9 | 55319 | 54309 | pdh | 0.0 | pyruvate dehydrogenase | 83.09 | 99.70 | ABP91006 |
| DIBLOPNL_2 | 152750 | 154111 | mnmE | 0.0 | central tRNA-modifying GTPase | 82.24 | 99.56 | ABP90420 |
| DIBLOPNL_9 | 4987 | 6879 | PepO | 0.0 | endopeptidase | 80.19 | 100.00 | ABF32980 |
| DIBLOPNL_1 | 137033 | 136143 | bglB | 1.62e-179 | putative phospho--glucosidase | 88.89 | 99.66 | AAK33487 |
| DIBLOPNL_11 | 12018 | 11080 | SP_0676 | 7.13e-169 | transcription facot | 81.15 | 97.20 | AAK74821 |
| DIBLOPNL_17 | 8925 | 8068 | metF | 1.50e-166 | methionine biosynthesis enzymes | 86.36 | 99.31 | AAK74739 |
| DIBLOPNL_2 | 29859 | 30563 | covR | 4.55e-132 | responder protein | 85.53 | 100.00 | AAK71319 |
| DIBLOPNL_3 | 31754 | 31083 | rr01 | 2.81e-126 | response regulator | 84.38 | 99.56 | CAB54566 |
| DIBLOPNL_17 | 2238 | 1570 | CiaR | 1.52e-124 | two-component response regulator | 86.10 | 99.55 | AAK74935 |
| DIBLOPNL_2 | 29859 | 30563 | walR | 1.92e-122 | DNA-binding response regulator | 82.13 | 100.00 | ANC99504 |
| DIBLOPNL_17 | 2238 | 1567 | ciaR | 2.06e-122 | two component system response regulator | 83.93 | 99.12 | AIK75708 |
| DIBLOPNL_7 | 7916 | 7230 | CovR | 3.45e-118 | serves to repress GAS virulence factor-encoding genes | 80.35 | 100.00 | ACE75886 |
| DIBLOPNL_14 | 41828 | 42202 | SpxA2 | 2.64e-71 | transcriptional regulator | 91.20 | 94.70 | ERL21461 |
| DIBLOPNL_2 | 141387 | 140986 | SpxA1 | 3.91e-67 | transcriptional regulator | 80.60 | 100.00 | ERL19273 |
| DIBLOPNL_16 | 13972 | 14259 | spr0479 | 3.02e-49 | essential gene | 86.46 | 98.97 | AAK99283 |
| DIBLOPNL_5 | 120813 | 121067 | spr1327 | 2.09e-45 | essential gene | 95.29 | 100.00 | AAL00131 |
| DIBLOPNL_8 | 76089 | 75826 | spr0175 | 3.53e-42 | pathogenitcity related | 84.09 | 100.00 | AAK98979 |
| DIBLOPNL_8 | 75262 | 74996 | spr0177 | 7.73e-34 | essential gene | 84.27 | 88.12 | AAK98981 |
| DIBLOPNL_10 | 7745 | 8245 | RopE (M5005_Spy_1611) | 1.77e-76 | probable DNA-directed RNA polymerase subunit delta | 81.44 | 87.43 | AAK34603 |
| DIBLOPNL_4 | 15598 | 13301 | PFL (B9H01_RS01125) | 0.0 | formate C-acetyltransferase | 82.94 | 98.08 | MYN69560 |
| DIBLOPNL_9 | 19456 | 20475 | ilvC (SPD_0406) | 0.0 | ketol-acid reductoisomerase (NADP(+)) | 96.47 | 100.00 | ABJ55451 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| DIBLOPNL_37 | 216 | 815 | repUS16 | 600 / 600 | 99.83 | GQ900438 |
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| DIBLOPNL_1 | 0.0 | 1.0 | 0.0 |
| DIBLOPNL_2 | 0.0 | 1.0 | 0.0 |
| DIBLOPNL_3 | 0.0 | 1.0 | 0.0 |
| DIBLOPNL_4 | 0.0 | 1.0 | 0.0 |
| DIBLOPNL_5 | 0.0 | 1.0 | 0.0 |
| DIBLOPNL_6 | 0.0 | 1.0 | 0.0 |
| DIBLOPNL_7 | 0.0 | 1.0 | 0.0 |
| DIBLOPNL_8 | 0.0 | 1.0 | 0.0 |
| DIBLOPNL_9 | 0.0 | 1.0 | 0.0 |
| DIBLOPNL_10 | 0.0 | 1.0 | 0.0 |
| DIBLOPNL_11 | 0.0 | 1.0 | 0.0 |
| DIBLOPNL_12 | 0.0 | 1.0 | 0.0 |
| DIBLOPNL_13 | 0.0 | 1.0 | 0.0 |
| DIBLOPNL_14 | 0.0 | 1.0 | 0.0 |
| DIBLOPNL_15 | 0.0 | 1.0 | 0.0 |
| DIBLOPNL_16 | 0.0 | 1.0 | 0.0 |
| DIBLOPNL_17 | 0.0 | 1.0 | 0.0 |
| DIBLOPNL_18 | 0.0 | 1.0 | 0.0 |
| DIBLOPNL_19 | 0.0 | 1.0 | 0.0 |
| DIBLOPNL_20 | 0.0 | 1.0 | 0.0 |
| DIBLOPNL_21 | 0.0 | 1.0 | 0.0 |
| DIBLOPNL_22 | 0.0 | 1.0 | 0.0 |
| DIBLOPNL_23 | 0.0 | 1.0 | 0.0 |
| DIBLOPNL_24 | 1.0 | 0.0 | 1.0 |
| DIBLOPNL_25 | 0.0 | 1.0 | 0.0 |
| DIBLOPNL_26 | 1.0 | 0.0 | 1.0 |
| DIBLOPNL_28 | 0.0 | 1.0 | 0.0 |
| DIBLOPNL_29 | 0.0 | 1.0 | 0.0 |
| DIBLOPNL_30 | 0.0 | 1.0 | 0.0 |
| DIBLOPNL_31 | 1.0 | 0.0 | 1.0 |
| DIBLOPNL_32 | 0.0 | 1.0 | 0.0 |
| DIBLOPNL_33 | 0.0 | 1.0 | 0.0 |
| DIBLOPNL_35 | 0.0 | 1.0 | 0.0 |
| DIBLOPNL_36 | 1.0 | 0.0 | 1.0 |
| DIBLOPNL_37 | 1.0 | 0.0 | 1.0 |
| DIBLOPNL_38 | 1.0 | 0.0 | 1.0 |
| DIBLOPNL_39 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| No hit found | |||||
Phigaro v2.4.0 (Database: Jan. 2024 release)