Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 12 | 10 | 4 | 16.12 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| BHPPKEPD_1 | 2708954 | 2709808 | aadK | ARO:3002627 | aadK | 97.89 | 100.00 | AL009126.1 |
| BHPPKEPD_1 | 2692121 | 2693323 | blt | ARO:3003006 | blt | 100.00 | 100.00 | L32599.1 |
| BHPPKEPD_1 | 2498616 | 2499785 | bmr | ARO:3003007 | bmr | 99.74 | 100.00 | M33768.1 |
| BHPPKEPD_1 | 1396548 | 1396865 | ykkD | ARO:3003064 | ykkD | 99.05 | 100.00 | AL009126.1 |
| BHPPKEPD_1 | 1396210 | 1396548 | ykkC | ARO:3003063 | ykkC | 100.00 | 100.00 | AL009126.1 |
| BHPPKEPD_1 | 922171 | 924741 | Bacillus subtilis mprF | ARO:3003324 | Bsub_mprF | 99.88 | 100.00 | AL009126.3 |
| BHPPKEPD_1 | 582349 | 583974 | vmlR | ARO:3004476 | vmlR | 96.86 | 98.90 | NC_000964.3 |
| BHPPKEPD_1 | 335047 | 335640 | tmrB | ARO:3003059 | tmrB | 98.48 | 100.00 | AL009126.3 |
| BHPPKEPD_1 | 284491 | 285924 | lmrB | ARO:3002813 | lmrB | 99.37 | 100.00 | JYFL01000006.1 |
| BHPPKEPD_1 | 271676 | 272596 | mphK | ARO:3004541 | mphK | 99.01 | 100.00 | CP010314.1 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| BHPPKEPD_1 | 2708954 | 2709808 | aadK | Streptomycin | 98.71 | 100.00 | M26879 |
| BHPPKEPD_1 | 271676 | 272596 | mph(K) | Spiramycin, Telithromycin | 98.59 | 100.00 | NC_000964 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| BHPPKEPD_1 | 271676 | 272584 | mphK | macrolide 2'-phosphotransferase MphK | BLASTX | 99.01 | 99.02 | WP_003246254.1 |
| BHPPKEPD_1 | 2708957 | 2709808 | aadK | aminoglycoside 6-adenylyltransferase AadK | BLASTX | 97.89 | 100.00 | WP_003229862.1 |
| BHPPKEPD_1 | 582352 | 583974 | vmlR | ABC-F type ribosomal protection protein VmlR | BLASTX | 96.86 | 98.90 | WP_003234144.1 |
| BHPPKEPD_1 | 4178206 | 4178724 | satA | streptothricin N-acetyltransferase SatA | BLASTX | 94.22 | 100.00 | WP_003242546.1 |
| BHPPKEPD_1 | 2070489 | 2073086 | rphC | rifamycin-inactivating phosphotransferase RphC | BLASTX | 80.60 | 100.00 | WP_087347987.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| BHPPKEPD_1 | 2301362 | 2302003 | hlyIII | 0.0 | Hemolysin III | Exotoxin | 99.84 | 100.00 | NP_390062 |
| BHPPKEPD_1 | 3668060 | 3669241 | capB | 0.0 | Capsule | Immune modulation | 99.75 | 100.00 | NP_391471 |
| BHPPKEPD_1 | 3666435 | 3667577 | capA | 0.0 | Capsule | Immune modulation | 99.74 | 100.00 | NP_391469 |
| BHPPKEPD_1 | 3244455 | 3245240 | dhbA | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.62 | 100.00 | NP_391080 |
| BHPPKEPD_1 | 3243233 | 3244429 | dhbC | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.50 | 100.00 | NP_391079 |
| BHPPKEPD_1 | 3240619 | 3241557 | dhbB | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.15 | 100.00 | NP_391077 |
| BHPPKEPD_1 | 3667596 | 3668045 | capC | 0.0 | Capsule | Immune modulation | 99.11 | 100.00 | NP_391470 |
| BHPPKEPD_1 | 3233463 | 3240599 | dhbF | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.95 | 100.00 | NP_391076 |
| BHPPKEPD_1 | 2024755 | 2026518 | dep/capD | 0.0 | Capsule | Immune modulation | 98.92 | 100.00 | NP_389723 |
| BHPPKEPD_1 | 3241585 | 3243204 | dhbE | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.58 | 100.00 | NP_391078 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| BHPPKEPD_1 | 132600 | 133736 | tufA | 0.0 | elongation factor Tu | 84.96 | 95.69 | AHM69299 |
| BHPPKEPD_1 | 18787 | 19656 | pdxS | 3.13e-149 | encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS | 83.79 | 98.31 | ACE61268 |
| BHPPKEPD_1 | 1708707 | 1709483 | CodY | 3.42e-124 | nutrient-responsive regulator | 81.08 | 100.00 | ACJ80679 |
| BHPPKEPD_1 | 3510022 | 3510591 | ClpP | 2.53e-98 | part of proteolytic complex | 81.05 | 97.44 | BAB94595 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| BHPPKEPD_1 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| BHPPKEPD_1 | 627996 | 637899 | False | Siphoviridae | VOG6513, VOG0275, VOG4602, VOG9607, VOG9667, VOG4603, VOG0650, VOG4552, VOG4603, VOG8233, VOG0186 |
| BHPPKEPD_1 | 646954 | 670326 | False | Siphoviridae | VOG0796, VOG10227, VOG0720, VOG4564, VOG4555, VOG4553, VOG1125, VOG0815, VOG4623, VOG0816, VOG5660, VOG0724, VOG0799, VOG2621, VOG0725, VOG4545, VOG4605, VOG4599, VOG1799, VOG5535, VOG0753, VOG0136, VOG4574 |
| BHPPKEPD_1 | 1337973 | 1365255 | False | Myoviridae | VOG4602, VOG9667, VOG3490, VOG0189, VOG4861, VOG5447, VOG0796, VOG10227, VOG4773, VOG4685, VOG0749, VOG3890, VOG3891, VOG3892, VOG6163, VOG4865, VOG4885, VOG3894, VOG4550, VOG4691, VOG4862, VOG7718, VOG4705 |
| BHPPKEPD_1 | 2184073 | 2193134 | False | Myoviridae / Siphoviridae | VOG4724, VOG4577, VOG4692, VOG4551, VOG0025, VOG7627, VOG0322 |
| BHPPKEPD_1 | 2239055 | 2271026 | False | Siphoviridae | VOG5721, VOG10940, VOG7134, VOG9867, VOG6036, VOG5495, VOG9219, VOG9713, VOG6940, VOG5534, VOG7718, VOG8610, VOG7930, VOG0275, VOG4052, VOG4910, VOG3389, VOG5042, VOG9712, VOG4626, VOG3390, VOG4705, VOG4574 |
Phigaro v2.4.0 (Database: Jan. 2024 release)