safety

PPRS ARGs VFs PGs Plasmid Prophage IS elements

ARGs	VFs	PGs	PPRS
4	0	1	4.12

PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).

Comprehensive Antibiotic Resistance Database (CARD)

Query ID	Begin	End	ARO name	ARO accession	CARD name	Identity	Coverage	Accession no.
JMKPCMNG_30	185373	186110	ErmB	ARO:3000375	ErmB	98.37	98.79	AF242872.1
JMKPCMNG_13	150927	152132	Mef(En2)	ARO:3004659	Mef(En2)	99.25	100.00	AF251288.1

RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)

ResFinder

Query ID	Begin	End	Resistance gene	Phenotype	Identity	Coverage	Accession no.
JMKPCMNG_30	185373	186110	erm(B)	Erythromycin, Lincomycin, Clindamycin, Quinupristin, Pristinamycin IA, Virginiamycin S	99.59	100.00	U18931

ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)

AMRFinderPlus

Query ID	Begin	End	Gene symbol	Sequence name	Method	Identity	Coverage	Accession no.
JMKPCMNG_7	450775	451680	blaMUN-1	MUN family extended-spectrum class A beta-lactamase MUN-1	ALLELEX	100.00	100.00	WP_206340447.1
JMKPCMNG_13	150393	150902	lnu(AN2)	lincosamide nucleotidyltransferase Lnu(AN2)	EXACTX	100.00	100.00	WP_004308783.1
JMKPCMNG_13	150930	152132	mef(En2)	macrolide efflux MFS transporter Mef(En2)	BLASTX	99.25	100.00	WP_063853729.1
JMKPCMNG_30	185373	186107	erm(B)	23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(B)	BLASTX	98.78	100.00	WP_002292226.1

AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)

Virulence Factor Database (VFDB)

Query ID	qstart	qend	Gene name	e-value	VF name	VF category	Identity	Coverage	Accession no.
No hit found

BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)

VirulenceFinder

Query ID	Begin	End	Database	Virulence factor	Protein function	Identity	Coverage	Accession no.
No hit found

VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)

PHI-base

Query ID	qstart	qend	Gene name	e-value	Function	Identity	Coverage	Gene ID
JMKPCMNG_36	6510	4930	GroEL	0.0	folding of newly synthesized proteins, preventing misfolding and aggregation	80.64	96.70	BAA04222

BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)

PlasmidFinder

Query ID	Begin	End	Plasmid	Query / Template	Identity	Accession no.
No hit found

PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)

PlasmidHunter

Query ID	Prediction	Chromosome	Plasmid
JMKPCMNG_1	0.0	1.0	0.0
JMKPCMNG_2	0.0	1.0	0.0
JMKPCMNG_3	0.0	1.0	0.0
JMKPCMNG_4	0.0	1.0	0.0
JMKPCMNG_5	0.0	1.0	0.0
JMKPCMNG_6	0.0	1.0	0.0
JMKPCMNG_7	0.0	1.0	0.0
JMKPCMNG_8	0.0	1.0	0.0
JMKPCMNG_9	1.0	0.0	1.0
JMKPCMNG_10	0.0	1.0	0.0
JMKPCMNG_11	0.0	1.0	0.0
JMKPCMNG_12	0.0	1.0	0.0
JMKPCMNG_13	0.0	1.0	0.0
JMKPCMNG_14	1.0	0.0	1.0
JMKPCMNG_15	0.0	1.0	0.0
JMKPCMNG_16	0.0	1.0	0.0
JMKPCMNG_17	0.0	1.0	0.0
JMKPCMNG_19	0.0	1.0	0.0
JMKPCMNG_20	0.0	1.0	0.0
JMKPCMNG_21	0.0	1.0	0.0
JMKPCMNG_22	0.0	1.0	0.0
JMKPCMNG_23	0.0	1.0	0.0
JMKPCMNG_24	0.0	1.0	0.0
JMKPCMNG_25	1.0	0.0	1.0
JMKPCMNG_26	0.0	1.0	0.0
JMKPCMNG_27	0.0	1.0	0.0
JMKPCMNG_28	0.0	1.0	0.0
JMKPCMNG_29	1.0	0.0	1.0
JMKPCMNG_30	0.0	1.0	0.0
JMKPCMNG_31	0.0	1.0	0.0
JMKPCMNG_32	0.0	1.0	0.0
JMKPCMNG_33	0.0	1.0	0.0
JMKPCMNG_34	0.0	1.0	0.0
JMKPCMNG_35	0.0	1.0	0.0
JMKPCMNG_36	0.0	1.0	0.0

PlasmidHunter v 1.4.5 (Database: May. 2024 release)

Phigaro

Query ID	Begin	End	Transposable	Taxonomy	pVOGs
JMKPCMNG_3	49834	59585	True	Unknown	VOG8336,

Phigaro v2.4.0 (Database: Jan. 2024 release)