safety

PPRS ARGs VFs PGs Plasmid Prophage IS elements

ARGs	VFs	PGs	PPRS
13	10	4	16.88

PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).

Comprehensive Antibiotic Resistance Database (CARD)

Query ID	Begin	End	ARO name	ARO accession	CARD name	Identity	Coverage	Accession no.
AADHFPHB_1	2735346	2736200	aadK	ARO:3002627	aadK	100.00	100.00	AL009126.1
AADHFPHB_1	2716637	2717839	blt	ARO:3003006	blt	100.00	100.00	L32599.1
AADHFPHB_1	2494318	2495487	bmr	ARO:3003007	bmr	99.74	100.00	M33768.1
AADHFPHB_1	1376521	1376838	ykkD	ARO:3003064	ykkD	100.00	100.00	AL009126.1
AADHFPHB_1	1376183	1376521	ykkC	ARO:3003063	ykkC	100.00	100.00	AL009126.1
AADHFPHB_1	916437	919007	Bacillus subtilis mprF	ARO:3003324	Bsub_mprF	100.00	100.00	AL009126.3
AADHFPHB_1	604382	606025	vmlR	ARO:3004476	vmlR	100.00	100.00	NC_000964.3
AADHFPHB_1	338803	339396	tmrB	ARO:3003059	tmrB	100.00	100.00	AL009126.3
AADHFPHB_1	288300	289733	lmrB	ARO:3002813	lmrB	100.00	100.00	JYFL01000006.1
AADHFPHB_1	275485	276405	mphK	ARO:3004541	mphK	100.00	100.00	CP010314.1

RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)

ResFinder

Query ID	Begin	End	Resistance gene	Phenotype	Identity	Coverage	Accession no.
AADHFPHB_1	2735346	2736200	aadK	Streptomycin	100.00	100.00	M26879
AADHFPHB_1	275485	276405	mph(K)	Spiramycin, Telithromycin	100.00	100.00	NC_000964
AADHFPHB_1	4187405	4188781	tet(L)	Doxycycline, Tetracycline, Minocycline, Tigecycline, Doxycycline, Tetracycline	100.00	100.00	X08034

ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)

AMRFinderPlus

Query ID	Begin	End	Gene symbol	Sequence name	Method	Identity	Coverage	Accession no.
AADHFPHB_1	2735349	2736200	aadK	aminoglycoside 6-adenylyltransferase AadK	EXACTX	100.00	100.00	WP_003229862.1
AADHFPHB_1	275485	276402	mphK	macrolide 2'-phosphotransferase MphK	EXACTX	100.00	100.00	WP_003246254.1
AADHFPHB_1	4184887	4185405	satA	streptothricin N-acetyltransferase SatA	EXACTX	100.00	100.00	WP_003242546.1
AADHFPHB_1	4187408	4188781	tet(L)	tetracycline efflux MFS transporter Tet(L)	EXACTX	100.00	100.00	WP_003242953.1
AADHFPHB_1	604385	606025	vmlR	ABC-F type ribosomal protection protein VmlR	EXACTX	100.00	100.00	WP_003234144.1
AADHFPHB_1	2051003	2053597	rphC	rifamycin-inactivating phosphotransferase RphC	BLASTX	81.15	99.88	WP_087347987.1

AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)

Virulence Factor Database (VFDB)

Query ID	qstart	qend	Gene name	VF name	VF category	Identity	Coverage	Accession no.
AADHFPHB_1	3697516	3698658	capA	Capsule	Immune modulation	100.00	100.00	NP_391469
AADHFPHB_1	3699141	3700322	capB	Capsule	Immune modulation	100.00	100.00	NP_391471
AADHFPHB_1	3698677	3699126	capC	Capsule	Immune modulation	100.00	100.00	NP_391470
AADHFPHB_1	2004345	2006108	dep/capD	Capsule	Immune modulation	100.00	100.00	NP_389723
AADHFPHB_1	3291181	3291966	dhbA	Bacillibactin	Nutritional/Metabolic factor	100.00	100.00	NP_391080
AADHFPHB_1	3287345	3288283	dhbB	Bacillibactin	Nutritional/Metabolic factor	100.00	100.00	NP_391077
AADHFPHB_1	3289959	3291155	dhbC	Bacillibactin	Nutritional/Metabolic factor	100.00	100.00	NP_391079
AADHFPHB_1	3288311	3289930	dhbE	Bacillibactin	Nutritional/Metabolic factor	100.00	100.00	NP_391078
AADHFPHB_1	3280189	3287325	dhbF	Bacillibactin	Nutritional/Metabolic factor	100.00	100.00	NP_391076
AADHFPHB_1	2294971	2295612	hlyIII	Hemolysin III	Exotoxin	100.00	100.00	NP_390062

BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)

VirulenceFinder

Query ID	Begin	End	Database	Virulence factor	Protein function	Identity	Coverage	Accession no.
No hit found

VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)

PHI-base

Query ID	qstart	qend	Gene name	e-value	Function	Identity	Coverage	Gene ID
AADHFPHB_1	132502	133638	tufA	0.0	elongation factor Tu	84.96	95.69	AHM69299
AADHFPHB_1	18663	19532	pdxS	3.13e-149	encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS	83.79	98.31	ACE61268
AADHFPHB_1	1689785	1690561	CodY	3.42e-124	nutrient-responsive regulator	81.08	100.00	ACJ80679
AADHFPHB_1	3545904	3546473	ClpP	2.54e-98	part of proteolytic complex	81.05	97.44	BAB94595

BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)

PlasmidFinder

Query ID	Begin	End	Plasmid	Query / Template	Identity	Accession no.
No hit found

PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)

PlasmidHunter

Query ID	Prediction	Chromosome	Plasmid
AADHFPHB_1	0.0	1.0	0.0

PlasmidHunter v 1.4.5 (Database: May. 2024 release)

Phigaro

Query ID	Begin	End	Transposable	Taxonomy	pVOGs
AADHFPHB_1	1320236	1346344	False	Myoviridae	VOG4602, VOG9667, VOG3490, VOG0189, VOG4861, VOG5447, VOG0796, VOG10227, VOG4773, VOG4685, VOG0749, VOG3890, VOG3891, VOG3892, VOG6163, VOG4865, VOG4885, VOG3894, VOG4550, VOG4691, VOG4862, VOG7718, VOG4705
AADHFPHB_1	2666708	2696952	False	Myoviridae	VOG7718, VOG4862, VOG4691, VOG4550, VOG3894, VOG4885, VOG4865, VOG6163, VOG3892, VOG3891, VOG3890, VOG10055, VOG4870, VOG4589, VOG0749, VOG4685, VOG4688, VOG4773, VOG10227, VOG0796, VOG4673, VOG9587, VOG4693, VOG0189, VOG11060, VOG1309, VOG4600

Phigaro v2.4.0 (Database: Jan. 2024 release)