safety

PPRS ARGs VFs PGs Plasmid Prophage IS elements

ARGs	VFs	PGs	PPRS
13	10	4	16.88

PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).

Comprehensive Antibiotic Resistance Database (CARD)

Query ID	Begin	End	ARO name	ARO accession	CARD name	Identity	Coverage	Accession no.
DEMIHHGF_1	2735272	2736126	aadK	ARO:3002627	aadK	100.00	100.00	AL009126.1
DEMIHHGF_1	2716563	2717765	blt	ARO:3003006	blt	100.00	100.00	L32599.1
DEMIHHGF_1	2494246	2495415	bmr	ARO:3003007	bmr	99.74	100.00	M33768.1
DEMIHHGF_1	1376449	1376766	ykkD	ARO:3003064	ykkD	100.00	100.00	AL009126.1
DEMIHHGF_1	1376111	1376449	ykkC	ARO:3003063	ykkC	100.00	100.00	AL009126.1
DEMIHHGF_1	916373	918943	Bacillus subtilis mprF	ARO:3003324	Bsub_mprF	100.00	100.00	AL009126.3
DEMIHHGF_1	604334	605977	vmlR	ARO:3004476	vmlR	100.00	100.00	NC_000964.3
DEMIHHGF_1	338755	339348	tmrB	ARO:3003059	tmrB	100.00	100.00	AL009126.3
DEMIHHGF_1	288252	289685	lmrB	ARO:3002813	lmrB	100.00	100.00	JYFL01000006.1
DEMIHHGF_1	275437	276357	mphK	ARO:3004541	mphK	100.00	100.00	CP010314.1

RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)

ResFinder

Query ID	Begin	End	Resistance gene	Phenotype	Identity	Coverage	Accession no.
DEMIHHGF_1	2735272	2736126	aadK	Streptomycin	100.00	100.00	M26879
DEMIHHGF_1	275437	276357	mph(K)	Spiramycin, Telithromycin	100.00	100.00	NC_000964
DEMIHHGF_1	4187272	4188648	tet(L)	Doxycycline, Tetracycline, Doxycycline, Tetracycline, Minocycline, Tigecycline	100.00	100.00	X08034

ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)

AMRFinderPlus

Query ID	Begin	End	Gene symbol	Sequence name	Method	Identity	Coverage	Accession no.
DEMIHHGF_1	2735275	2736126	aadK	aminoglycoside 6-adenylyltransferase AadK	EXACTX	100.00	100.00	WP_003229862.1
DEMIHHGF_1	275437	276354	mphK	macrolide 2'-phosphotransferase MphK	EXACTX	100.00	100.00	WP_003246254.1
DEMIHHGF_1	4184754	4185272	satA	streptothricin N-acetyltransferase SatA	EXACTX	100.00	100.00	WP_003242546.1
DEMIHHGF_1	4187275	4188648	tet(L)	tetracycline efflux MFS transporter Tet(L)	EXACTX	100.00	100.00	WP_003242953.1
DEMIHHGF_1	604337	605977	vmlR	ABC-F type ribosomal protection protein VmlR	EXACTX	100.00	100.00	WP_003234144.1
DEMIHHGF_1	2050929	2053523	rphC	rifamycin-inactivating phosphotransferase RphC	BLASTX	81.15	99.88	WP_087347987.1

AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)

Virulence Factor Database (VFDB)

Query ID	qstart	qend	Gene name	VF name	VF category	Identity	Coverage	Accession no.
DEMIHHGF_1	3697411	3698553	capA	Capsule	Immune modulation	100.00	100.00	NP_391469
DEMIHHGF_1	3699036	3700217	capB	Capsule	Immune modulation	100.00	100.00	NP_391471
DEMIHHGF_1	3698572	3699021	capC	Capsule	Immune modulation	100.00	100.00	NP_391470
DEMIHHGF_1	2004271	2006034	dep/capD	Capsule	Immune modulation	100.00	100.00	NP_389723
DEMIHHGF_1	3291101	3291886	dhbA	Bacillibactin	Nutritional/Metabolic factor	100.00	100.00	NP_391080
DEMIHHGF_1	3287265	3288203	dhbB	Bacillibactin	Nutritional/Metabolic factor	100.00	100.00	NP_391077
DEMIHHGF_1	3289879	3291075	dhbC	Bacillibactin	Nutritional/Metabolic factor	100.00	100.00	NP_391079
DEMIHHGF_1	3288231	3289850	dhbE	Bacillibactin	Nutritional/Metabolic factor	100.00	100.00	NP_391078
DEMIHHGF_1	3280109	3287245	dhbF	Bacillibactin	Nutritional/Metabolic factor	100.00	100.00	NP_391076
DEMIHHGF_1	2294894	2295535	hlyIII	Hemolysin III	Exotoxin	100.00	100.00	NP_390062

BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)

VirulenceFinder

Query ID	Begin	End	Database	Virulence factor	Protein function	Identity	Coverage	Accession no.
No hit found

VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)

PHI-base

Query ID	qstart	qend	Gene name	e-value	Function	Identity	Coverage	Gene ID
DEMIHHGF_1	132473	133609	tufA	0.0	elongation factor Tu	84.96	95.69	AHM69299
DEMIHHGF_1	18665	19534	pdxS	3.13e-149	encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS	83.79	98.31	ACE61268
DEMIHHGF_1	1689713	1690489	CodY	3.42e-124	nutrient-responsive regulator	81.08	100.00	ACJ80679
DEMIHHGF_1	3545824	3546393	ClpP	2.54e-98	part of proteolytic complex	81.05	97.44	BAB94595

BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)

PlasmidFinder

Query ID	Begin	End	Plasmid	Query / Template	Identity	Accession no.
No hit found

PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)

PlasmidHunter

Query ID	Prediction	Chromosome	Plasmid
DEMIHHGF_1	0.0	1.0	0.0

PlasmidHunter v 1.4.5 (Database: May. 2024 release)

Phigaro

Query ID	Begin	End	Transposable	Taxonomy	pVOGs
DEMIHHGF_1	1320164	1346272	False	Myoviridae	VOG4602, VOG9667, VOG3490, VOG0189, VOG4861, VOG5447, VOG0796, VOG10227, VOG4773, VOG4685, VOG0749, VOG3890, VOG3891, VOG3892, VOG6163, VOG4865, VOG4885, VOG3894, VOG4550, VOG4691, VOG4862, VOG7718, VOG4705
DEMIHHGF_1	2666635	2696879	False	Myoviridae	VOG7718, VOG4862, VOG4691, VOG4550, VOG3894, VOG4885, VOG4865, VOG6163, VOG3892, VOG3891, VOG3890, VOG10055, VOG4870, VOG4589, VOG0749, VOG4685, VOG4688, VOG4773, VOG10227, VOG0796, VOG4673, VOG9587, VOG4693, VOG0189, VOG11060, VOG1309, VOG4600

Phigaro v2.4.0 (Database: Jan. 2024 release)