Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 13 | 10 | 9 | 18.71 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| BAGKBOMJ_4 | 337289 | 338209 | mphK | ARO:3004541 | mphK | 97.71 | 100.00 | CP010314.1 |
| BAGKBOMJ_4 | 323956 | 325395 | lmrB | ARO:3002813 | lmrB | 99.37 | 100.42 | JYFL01000006.1 |
| BAGKBOMJ_2 | 292326 | 293495 | bmr | ARO:3003007 | bmr | 99.74 | 100.00 | M33768.1 |
| BAGKBOMJ_4 | 274321 | 274914 | tmrB | ARO:3003059 | tmrB | 98.48 | 100.00 | AL009126.3 |
| BAGKBOMJ_9 | 270886 | 273456 | Bacillus subtilis mprF | ARO:3003324 | Bsub_mprF | 99.88 | 100.00 | AL009126.3 |
| BAGKBOMJ_1 | 74479 | 74817 | ykkC | ARO:3003063 | ykkC | 100.00 | 100.00 | AL009126.1 |
| BAGKBOMJ_1 | 74162 | 74479 | ykkD | ARO:3003064 | ykkD | 99.05 | 100.00 | AL009126.1 |
| BAGKBOMJ_4 | 29195 | 30838 | vmlR | ARO:3004476 | vmlR | 100.00 | 100.00 | NC_000964.3 |
| BAGKBOMJ_14 | 26494 | 27696 | blt | ARO:3003006 | blt | 100.00 | 100.00 | L32599.1 |
| BAGKBOMJ_3 | 5350 | 6204 | aadK | ARO:3002627 | aadK | 100.00 | 100.00 | AL009126.1 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| BAGKBOMJ_3 | 5350 | 6204 | aadK | Streptomycin | 100.00 | 100.00 | M26879 |
| BAGKBOMJ_7 | 36466 | 37842 | tet(L) | Doxycycline, Tetracycline, Doxycycline, Tetracycline, Minocycline, Tigecycline | 99.85 | 100.00 | X08034 |
| BAGKBOMJ_4 | 337289 | 338209 | mph(K) | Spiramycin, Telithromycin | 97.72 | 100.00 | NC_000964 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| BAGKBOMJ_3 | 5353 | 6204 | aadK | aminoglycoside 6-adenylyltransferase AadK | EXACTX | 100.00 | 100.00 | WP_003229862.1 |
| BAGKBOMJ_7 | 36466 | 37839 | tet(L) | tetracycline efflux MFS transporter Tet(L) | EXACTX | 100.00 | 100.00 | WP_003242953.1 |
| BAGKBOMJ_4 | 29195 | 30835 | vmlR | ABC-F type ribosomal protection protein VmlR | EXACTX | 100.00 | 100.00 | WP_003234144.1 |
| BAGKBOMJ_4 | 337292 | 338209 | mphK | macrolide 2'-phosphotransferase MphK | BLASTX | 97.71 | 100.00 | WP_003246254.1 |
| BAGKBOMJ_7 | 39795 | 40313 | satA | streptothricin N-acetyltransferase SatA | BLASTX | 90.17 | 100.00 | WP_003242546.1 |
| BAGKBOMJ_10 | 11551 | 14145 | rphC | rifamycin-inactivating phosphotransferase RphC | BLASTX | 81.15 | 99.88 | WP_087347987.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| BAGKBOMJ_10 | 59039 | 60802 | dep/capD | 0.0 | Capsule | Immune modulation | 100.00 | 100.00 | NP_389723 |
| BAGKBOMJ_2 | 92949 | 93590 | hlyIII | 0.0 | Hemolysin III | Exotoxin | 100.00 | 100.00 | NP_390062 |
| BAGKBOMJ_8 | 112674 | 113459 | dhbA | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.75 | 100.00 | NP_391080 |
| BAGKBOMJ_8 | 108838 | 109776 | dhbB | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.68 | 100.00 | NP_391077 |
| BAGKBOMJ_5 | 155966 | 157147 | capB | 0.0 | Capsule | Immune modulation | 99.66 | 100.00 | NP_391471 |
| BAGKBOMJ_8 | 109804 | 111423 | dhbE | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.26 | 100.00 | NP_391078 |
| BAGKBOMJ_5 | 157630 | 158772 | capA | 0.0 | Capsule | Immune modulation | 98.95 | 100.00 | NP_391469 |
| BAGKBOMJ_8 | 101682 | 108818 | dhbF | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.95 | 100.00 | NP_391076 |
| BAGKBOMJ_8 | 111452 | 112648 | dhbC | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.91 | 100.00 | NP_391079 |
| BAGKBOMJ_5 | 157162 | 157611 | capC | 0.0 | Capsule | Immune modulation | 98.67 | 100.00 | NP_391470 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| BAGKBOMJ_12 | 31783 | 32919 | tufA | 0.0 | elongation factor Tu | 84.96 | 95.69 | AHM69299 |
| BAGKBOMJ_18 | 4391 | 5260 | pdxS | 2.60e-151 | encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS | 83.45 | 98.31 | ACE61268 |
| BAGKBOMJ_6 | 196423 | 197199 | CodY | 3.11e-125 | nutrient-responsive regulator | 81.08 | 100.00 | ACJ80679 |
| BAGKBOMJ_5 | 310236 | 309667 | ClpP | 2.34e-99 | part of proteolytic complex | 81.05 | 97.44 | BAB94595 |
| BAGKBOMJ_1 | 225606 | 225214 | spxA1 | 3.20e-70 | redox-responsive transcription factor | 83.97 | 100.00 | AAF21893 |
| BAGKBOMJ_1 | 225606 | 225214 | spx | 1.91e-68 | essential gene which is a thiol/oxidative stress sensor | 80.15 | 100.00 | BAB57159 |
| BAGKBOMJ_13 | 20625 | 20867 | SpoVG | 7.52e-39 | RNA-binding protein | 81.48 | 79.41 | AAT02994 |
| BAGKBOMJ_4 | 76716 | 76519 | CspA | 1.89e-31 | cold shock protein | 90.91 | 100.00 | CAA62903 |
| BAGKBOMJ_2 | 105552 | 105746 | CspD | 3.00e-28 | cold shock protein | 81.54 | 98.48 | CAC99957 |
| BAGKBOMJ_1 | 468838 | 469032 | CspB | 4.61e-27 | cold shock protein | 83.08 | 98.48 | CAD00094 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| BAGKBOMJ_1 | 0.0 | 1.0 | 0.0 |
| BAGKBOMJ_2 | 0.0 | 1.0 | 0.0 |
| BAGKBOMJ_3 | 0.0 | 1.0 | 0.0 |
| BAGKBOMJ_4 | 0.0 | 1.0 | 0.0 |
| BAGKBOMJ_5 | 0.0 | 1.0 | 0.0 |
| BAGKBOMJ_6 | 0.0 | 1.0 | 0.0 |
| BAGKBOMJ_7 | 0.0 | 1.0 | 0.0 |
| BAGKBOMJ_8 | 0.0 | 1.0 | 0.0 |
| BAGKBOMJ_9 | 0.0 | 1.0 | 0.0 |
| BAGKBOMJ_10 | 0.0 | 1.0 | 0.0 |
| BAGKBOMJ_11 | 0.0 | 1.0 | 0.0 |
| BAGKBOMJ_12 | 0.0 | 1.0 | 0.0 |
| BAGKBOMJ_13 | 0.0 | 1.0 | 0.0 |
| BAGKBOMJ_14 | 0.0 | 1.0 | 0.0 |
| BAGKBOMJ_15 | 0.0 | 1.0 | 0.0 |
| BAGKBOMJ_16 | 0.0 | 1.0 | 0.0 |
| BAGKBOMJ_17 | 0.0 | 1.0 | 0.0 |
| BAGKBOMJ_18 | 0.0 | 1.0 | 0.0 |
| BAGKBOMJ_19 | 0.0 | 1.0 | 0.0 |
| BAGKBOMJ_20 | 0.0 | 1.0 | 0.0 |
| BAGKBOMJ_21 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| BAGKBOMJ_1 | 104618 | 129970 | False | Myoviridae | VOG4705, VOG7718, VOG4862, VOG4691, VOG4550, VOG3894, VOG4885, VOG4865, VOG6163, VOG3892, VOG3891, VOG3890, VOG0749, VOG4685, VOG4773, VOG10227, VOG0796, VOG5447, VOG4861, VOG0189, VOG3490 |
| BAGKBOMJ_2 | 17204 | 28257 | False | Myoviridae | VOG4570, VOG5722, VOG5779, VOG5721, VOG10940, VOG7134, VOG9867, VOG6036 |
| BAGKBOMJ_2 | 464709 | 481806 | False | Myoviridae | VOG7718, VOG4862, VOG4691, VOG4550, VOG3894, VOG4885, VOG4865, VOG6163, VOG3892, VOG3891, VOG3890, VOG10055, VOG4870, VOG4589 |
| BAGKBOMJ_8 | 273530 | 297423 | False | Siphoviridae | VOG4707, VOG0641, VOG4705, VOG3390, VOG4612, VOG4660, VOG4619, VOG0801, VOG6163, VOG0541, VOG4586, VOG0704, VOG0539, VOG4589, VOG0204, VOG4553, VOG4568, VOG4556, VOG4544, VOG4581, VOG4841 |
| BAGKBOMJ_8 | 306572 | 310520 | False | Siphoviridae | VOG1563, VOG0275 |
| BAGKBOMJ_11 | 72721 | 77453 | False | Unknown | VOG0025, VOG7627, VOG0322 |
Phigaro v2.4.0 (Database: Jan. 2024 release)