Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 10 | 10 | 4 | 14.70 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| OIJMMPIG_1 | 3903962 | 3905395 | lmrB | ARO:3002813 | lmrB | 98.95 | 100.00 | JYFL01000006.1 |
| OIJMMPIG_1 | 3854088 | 3854681 | tmrB | ARO:3003059 | tmrB | 99.49 | 100.00 | AL009126.3 |
| OIJMMPIG_1 | 3305811 | 3308381 | Bacillus subtilis mprF | ARO:3003324 | Bsub_mprF | 99.77 | 100.00 | AL009126.3 |
| OIJMMPIG_1 | 2811043 | 2811381 | ykkC | ARO:3003063 | ykkC | 98.21 | 100.00 | AL009126.1 |
| OIJMMPIG_1 | 2810726 | 2811043 | ykkD | ARO:3003064 | ykkD | 98.10 | 100.00 | AL009126.1 |
| OIJMMPIG_1 | 1680216 | 1681070 | aadK | ARO:3002627 | aadK | 98.94 | 100.00 | AL009126.1 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| OIJMMPIG_1 | 1680216 | 1681070 | aadK | Streptomycin | 98.83 | 100.00 | M26879 |
| OIJMMPIG_1 | 3917290 | 3918048 | mph(K) | Spiramycin, Telithromycin | 97.63 | 82.41 | NC_000964 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| OIJMMPIG_1 | 1680216 | 1681067 | aadK | aminoglycoside 6-adenylyltransferase AadK | BLASTX | 98.94 | 100.00 | WP_003229862.1 |
| OIJMMPIG_1 | 3917302 | 3918048 | mphK | macrolide 2'-phosphotransferase MphK | PARTIALX | 98.39 | 81.37 | WP_003246254.1 |
| OIJMMPIG_1 | 3598211 | 3599818 | vmlR | ABC-F type ribosomal protection protein VmlR | INTERNAL_STOP | 95.61 | 100.00 | WP_003234144.1 |
| OIJMMPIG_1 | 28744 | 29262 | satA | streptothricin N-acetyltransferase SatA | BLASTX | 91.91 | 100.00 | WP_003242546.1 |
| OIJMMPIG_1 | 2351502 | 2354099 | rphC | rifamycin-inactivating phosphotransferase RphC | BLASTX | 80.48 | 100.00 | WP_087347987.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| OIJMMPIG_1 | 518094 | 519275 | capB | 0.0 | Capsule | Immune modulation | 99.49 | 100.00 | NP_391471 |
| OIJMMPIG_1 | 985378 | 986163 | dhbA | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.49 | 100.00 | NP_391080 |
| OIJMMPIG_1 | 519758 | 520900 | capA | 0.0 | Capsule | Immune modulation | 99.30 | 100.00 | NP_391469 |
| OIJMMPIG_1 | 989061 | 989999 | dhbB | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.15 | 100.00 | NP_391077 |
| OIJMMPIG_1 | 986189 | 987385 | dhbC | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.08 | 100.00 | NP_391079 |
| OIJMMPIG_1 | 2111566 | 2112207 | hlyIII | 0.0 | Hemolysin III | Exotoxin | 98.91 | 100.00 | NP_390062 |
| OIJMMPIG_1 | 519290 | 519739 | capC | 0.0 | Capsule | Immune modulation | 98.89 | 100.00 | NP_391470 |
| OIJMMPIG_1 | 990019 | 997155 | dhbF | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.66 | 100.00 | NP_391076 |
| OIJMMPIG_1 | 2298699 | 2300451 | dep/capD | 0.0 | Capsule | Immune modulation | 98.30 | 99.94 | NP_389723 |
| OIJMMPIG_1 | 987414 | 989033 | dhbE | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 97.04 | 100.00 | NP_391078 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| OIJMMPIG_1 | 4040176 | 4039040 | tufA | 0.0 | elongation factor Tu | 85.75 | 95.69 | AHM69299 |
| OIJMMPIG_1 | 4154072 | 4153203 | pdxS | 5.54e-149 | encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS | 83.45 | 98.31 | ACE61268 |
| OIJMMPIG_1 | 2500533 | 2499757 | CodY | 3.39e-124 | nutrient-responsive regulator | 81.08 | 100.00 | ACJ80679 |
| OIJMMPIG_1 | 674700 | 674131 | ClpP | 2.51e-98 | part of proteolytic complex | 81.05 | 97.44 | BAB94595 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| OIJMMPIG_1 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| OIJMMPIG_1 | 761269 | 771799 | True | Siphoviridae | VOG3490, VOG0275, VOG0275, VOG1563, VOG9667, VOG9667, VOG7581, VOG4566, VOG0189, VOG6587 |
| OIJMMPIG_1 | 772286 | 801360 | False | Siphoviridae | VOG1336, VOG0245, VOG5836, VOG0397, VOG0102, VOG0044, VOG0198, VOG0275, VOG4841, VOG0713, VOG4581, VOG1886, VOG3607, VOG4556, VOG4568, VOG4553, VOG10223, VOG0204, VOG9583, VOG0704, VOG4586, VOG0541, VOG5910, VOG0801, VOG4772, VOG6965, VOG4619, VOG4626, VOG4612, VOG6243, VOG4705 |
| OIJMMPIG_1 | 1375491 | 1409667 | False | Siphoviridae | VOG0189, VOG0536, VOG1336, VOG5836, VOG4567, VOG3444, VOG0397, VOG0044, VOG0198, VOG0275, VOG7884, VOG0713, VOG4841, VOG4581, VOG1886, VOG0202, VOG4556, VOG4573, VOG4553, VOG5361, VOG0204, VOG4589, VOG0539, VOG0704, VOG4586, VOG0541, VOG5910, VOG6163, VOG0801, VOG4619, VOG4626, VOG4612, VOG6243, VOG4705, VOG0641, VOG4574, VOG6116 |
| OIJMMPIG_1 | 1420230 | 1434331 | False | Siphoviridae | VOG0189, VOG5446, VOG0025, VOG4813, VOG4551, VOG2215, VOG1563, VOG0052, VOG6546, VOG5753, VOG10937, VOG5324, VOG7980, VOG0026 |
| OIJMMPIG_1 | 1454010 | 1477915 | False | Siphoviridae | VOG4841, VOG4581, VOG4544, VOG4556, VOG4573, VOG4553, VOG5601, VOG0204, VOG4589, VOG0539, VOG0704, VOG4586, VOG0541, VOG6163, VOG0801, VOG4619, VOG4626, VOG4612, VOG6243, VOG4705, VOG5221, VOG4574, VOG0136, VOG8440, VOG6116 |
| OIJMMPIG_1 | 1527625 | 1553073 | False | Siphoviridae | VOG0198, VOG3046, VOG10057, VOG4841, VOG4581, VOG1886, VOG0202, VOG4556, VOG4573, VOG4553, VOG5361, VOG0204, VOG4589, VOG0539, VOG0704, VOG4586, VOG0541, VOG5910, VOG6163, VOG0801, VOG4619, VOG4626, VOG4612, VOG6243, VOG4705, VOG0641 |
| OIJMMPIG_1 | 1896305 | 1906268 | False | Unknown | VOG6116, VOG6396, VOG7443, VOG3239, VOG0944, VOG0189, VOG3239, VOG6495 |
| OIJMMPIG_1 | 2840845 | 2868206 | False | Myoviridae | VOG0753, VOG4705, VOG7718, VOG4862, VOG4691, VOG4550, VOG3894, VOG4885, VOG4865, VOG6163, VOG3892, VOG3891, VOG3890, VOG0749, VOG4685, VOG4773, VOG10227, VOG0796, VOG5447, VOG4861, VOG0189, VOG3490, VOG9667 |
Phigaro v2.4.0 (Database: Jan. 2024 release)