Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 2 | 15 | 35 | 38.13 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| FLHEMEON_30 | 65178 | 66395 | mef(A) | Erythromycin, Azithromycin | 100.00 | 100.00 | U83667 |
| FLHEMEON_30 | 63595 | 65058 | msr(D) | Erythromycin, Azithromycin, Telithromycin, Quinupristin, Pristinamycin IA, Virginiamycin S | 100.00 | 100.00 | AF274302 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| FLHEMEON_30 | 65181 | 66395 | mef(A) | macrolide efflux MFS transporter Mef(A) | EXACTX | 100.00 | 100.00 | WP_000417519.1 |
| FLHEMEON_30 | 63598 | 65058 | msr(D) | ABC-F type ribosomal protection protein Msr(D) | EXACTX | 100.00 | 100.00 | WP_000420313.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| FLHEMEON_11 | 69315 | 70166 | rfbD | 0.0 | Capsule | Immune modulation | 95.07 | 100.00 | WP_014621820 |
| FLHEMEON_3 | 7646 | 9106 | STU_RS14650 | 0.0 | Capsule | Immune modulation | 93.77 | 100.14 | WP_011226049 |
| FLHEMEON_3 | 7646 | 9106 | STER_RS05320 | 0.0 | Capsule | Immune modulation | 93.70 | 100.00 | WP_011681177 |
| FLHEMEON_3 | 11347 | 12714 | STU_RS14630 | 0.0 | Capsule | Immune modulation | 93.06 | 100.00 | WP_011226045 |
| FLHEMEON_3 | 11347 | 12712 | STER_RS05300 | 0.0 | Capsule | Immune modulation | 92.90 | 99.85 | WP_011681173 |
| FLHEMEON_3 | 7646 | 9106 | STR_RS05265 | 0.0 | Capsule | Immune modulation | 92.88 | 100.14 | WP_041827043 |
| FLHEMEON_43 | 82154 | 82747 | STR_RS05880 | 0.0 | Capsule | Immune modulation | 92.09 | 100.00 | WP_011226149 |
| FLHEMEON_43 | 82154 | 82747 | STER_RS06030 | 0.0 | Capsule | Immune modulation | 91.92 | 100.00 | WP_011226149 |
| FLHEMEON_43 | 82154 | 82747 | STU_RS15260 | 0.0 | Capsule | Immune modulation | 91.92 | 100.00 | WP_011226149 |
| FLHEMEON_3 | 9107 | 9838 | STU_RS14645 | 0.0 | Capsule | Immune modulation | 91.80 | 100.00 | WP_011226048 |
| FLHEMEON_21 | 58194 | 58955 | srtA | 0.0 | Sortase A | Exoenzyme | 91.73 | 100.00 | - |
| FLHEMEON_43 | 81285 | 82154 | rfbA | 0.0 | Capsule | Immune modulation | 91.49 | 100.00 | WP_011681286 |
| FLHEMEON_3 | 9107 | 9838 | STER_RS05315 | 0.0 | Capsule | Immune modulation | 91.39 | 100.00 | WP_011681176 |
| FLHEMEON_3 | 9107 | 9838 | STR_RS05260 | 0.0 | Capsule | Immune modulation | 90.85 | 100.00 | WP_011227248 |
| FLHEMEON_3 | 10549 | 11289 | STER_RS05305 | 0.0 | Capsule | Immune modulation | 90.84 | 99.87 | WP_011681174 |
| FLHEMEON_21 | 205734 | 207392 | fbp54 | 0.0 | FBPs | Adherence | 90.42 | 99.94 | WP_011227179 |
| FLHEMEON_3 | 10549 | 11289 | STU_RS14635 | 0.0 | Capsule | Immune modulation | 89.89 | 99.87 | WP_011226046 |
| FLHEMEON_3 | 10549 | 11284 | STR_RS05250 | 0.0 | Capsule | Immune modulation | 89.55 | 98.00 | WP_011227246 |
| FLHEMEON_11 | 396152 | 397162 | plr/gapA | 0.0 | Streptococcal plasmin receptor/GAPDH | Adherence | 88.45 | 99.80 | WP_002986042 |
| FLHEMEON_30 | 95432 | 96735 | eno | 0.0 | Streptococcal enolase | Exoenzyme | 88.24 | 99.54 | WP_000022832 |
| FLHEMEON_3 | 9847 | 10539 | STU_RS14640 | 0.0 | Capsule | Immune modulation | 85.45 | 99.86 | WP_011226047 |
| FLHEMEON_3 | 9847 | 10539 | STR_RS05255 | 0.0 | Capsule | Immune modulation | 85.16 | 99.86 | WP_011227247 |
| FLHEMEON_3 | 9847 | 10539 | STER_RS05310 | 0.0 | Capsule | Immune modulation | 84.87 | 99.86 | WP_011681175 |
| FLHEMEON_44 | 31829 | 32725 | cbpD | 0.0 | Autolysin | Exoenzyme | 84.78 | 93.09 | WP_082302295 |
| FLHEMEON_33 | 203315 | 204598 | tig/ropA | 0.0 | Trigger factor | Stress survival | 80.84 | 99.61 | WP_000107753 |
| FLHEMEON_43 | 83019 | 84065 | rfbB | 0.0 | Capsule | Immune modulation | 80.13 | 100.00 | WP_009659423 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| FLHEMEON_21 | 55506 | 54523 | ldh(Sp_1220) | 0.0 | L-lactate dehydrogenase | 96.34 | 100.00 | AAK75326 |
| FLHEMEON_21 | 71768 | 72841 | MntE | 0.0 | manganese homeostasis | 95.25 | 99.72 | ABF32167 |
| FLHEMEON_11 | 247099 | 249405 | pfl2(Sp_0459) | 0.0 | formate acetyltransferase | 94.41 | 99.22 | AAK74619 |
| FLHEMEON_38 | 76733 | 75252 | SPD_0310 | 0.0 | UPF0371 protein SPD_0310 | 93.32 | 100.00 | ABJ54480 |
| FLHEMEON_30 | 186229 | 185261 | spr0084 | 0.0 | pathogenitcity related | 92.57 | 98.48 | AAK98888 |
| FLHEMEON_21 | 90823 | 93069 | metE | 0.0 | methionine biosynthesis enzymes | 92.52 | 100.00 | AAK74738 |
| FLHEMEON_4 | 38984 | 38013 | prsA2 | 0.0 | ribose-phosphate pyrophosphokinase | 91.67 | 100.00 | ABF36968 |
| FLHEMEON_43 | 55906 | 57636 | ptsI | 0.0 | phosphoenolpyruvate-protein phosphotransferase | 89.95 | 100.00 | AAK34199 |
| FLHEMEON_11 | 113608 | 112457 | potA | 0.0 | ABC transporter ATP-binding protein-spermidine/putrescine transport | 89.58 | 99.74 | AAL00050 |
| FLHEMEON_11 | 85126 | 83138 | uvrB | 0.0 | excinuclease ABC, B subunit | 89.44 | 100.00 | AE014259_2 |
| FLHEMEON_1 | 6917 | 8395 | IMPDH | 0.0 | inosine-5'-monophosphate dehydrogenase | 87.83 | 100.00 | ADE32434 |
| FLHEMEON_30 | 96735 | 95434 | Eno | 0.0 | enolase | 86.44 | 99.77 | ABP90468 |
| FLHEMEON_1 | 22280 | 24172 | gidA | 0.0 | tRNA uridine 5-carboxymethylaminomethyl modification enzyme | 85.74 | 98.90 | ABP91129 |
| FLHEMEON_21 | 95988 | 94279 | PGM | 0.0 | phosphoglucomutase | 85.11 | 99.65 | ACH87041 |
| FLHEMEON_4 | 1199 | 108 | recA | 0.0 | DNA recombination and repair | 84.64 | 95.36 | AAL00560 |
| FLHEMEON_42 | 28752 | 29762 | pdh | 0.0 | pyruvate dehydrogenase | 82.79 | 99.70 | ABP91006 |
| FLHEMEON_21 | 220899 | 219538 | mnmE | 0.0 | central tRNA-modifying GTPase | 82.46 | 99.56 | ABP90420 |
| FLHEMEON_30 | 111154 | 110264 | bglB | 2.61e-177 | putative phospho--glucosidase | 87.20 | 99.66 | AAK33487 |
| FLHEMEON_21 | 135117 | 134179 | SP_0676 | 2.46e-169 | transcription facot | 81.79 | 97.20 | AAK74821 |
| FLHEMEON_21 | 93256 | 94113 | metF | 7.24e-169 | methionine biosynthesis enzymes | 87.76 | 99.31 | AAK74739 |
| FLHEMEON_44 | 38874 | 38194 | NisR | 6.35e-133 | two-component signal-transduction systems | 94.71 | 100.00 | ABP89873 |
| FLHEMEON_44 | 38206 | 37502 | NisK | 1.55e-131 | two-component signal-transduction systems | 91.06 | 100.00 | ABP89872 |
| FLHEMEON_45 | 51841 | 52545 | covR | 3.99e-127 | responder protein | 85.53 | 100.00 | AAK71319 |
| FLHEMEON_38 | 142676 | 143347 | rr01 | 1.68e-125 | response regulator | 83.48 | 99.56 | CAB54566 |
| FLHEMEON_21 | 99957 | 100628 | ciaR | 2.75e-125 | two component system response regulator | 86.16 | 99.12 | AIK75708 |
| FLHEMEON_21 | 99957 | 100625 | CiaR | 5.09e-125 | two-component response regulator | 87.44 | 99.55 | AAK74935 |
| FLHEMEON_45 | 51841 | 52545 | walR | 9.07e-121 | DNA-binding response regulator | 82.13 | 100.00 | ANC99504 |
| FLHEMEON_33 | 53642 | 52956 | CovR | 4.51e-120 | serves to repress GAS virulence factor-encoding genes | 81.66 | 100.00 | ACE75886 |
| FLHEMEON_14 | 209 | 604 | SpxA2 | 1.26e-84 | transcriptional regulator | 91.67 | 100.00 | ERL21461 |
| FLHEMEON_21 | 232364 | 232765 | SpxA1 | 1.73e-69 | transcriptional regulator | 82.09 | 100.00 | ERL19273 |
| FLHEMEON_2 | 42869 | 43282 | GrpE | 3.83e-68 | protein grpE | 81.88 | 80.59 | ABP89267 |
| FLHEMEON_33 | 30435 | 30148 | spr0479 | 5.46e-48 | essential gene | 85.42 | 98.97 | AAK99283 |
| FLHEMEON_30 | 220257 | 220003 | spr1327 | 1.10e-44 | essential gene | 92.94 | 100.00 | AAL00131 |
| FLHEMEON_42 | 151357 | 151620 | spr0175 | 8.52e-43 | pathogenitcity related | 85.23 | 100.00 | AAK98979 |
| FLHEMEON_42 | 152181 | 152447 | spr0177 | 1.89e-41 | essential gene | 83.15 | 88.12 | AAK98981 |
| FLHEMEON_33 | 203148 | 202657 | RopE (M5005_Spy_1611) | 9.98e-73 | probable DNA-directed RNA polymerase subunit delta | 82.93 | 85.86 | AAK34603 |
| FLHEMEON_11 | 247099 | 249399 | PFL (B9H01_RS01125) | 0.0 | formate C-acetyltransferase | 83.22 | 98.08 | MYN69560 |
| FLHEMEON_42 | 65183 | 64164 | ilvC (SPD_0406) | 0.0 | ketol-acid reductoisomerase (NADP(+)) | 97.35 | 100.00 | ABJ55451 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| FLHEMEON_13 | 3634 | 4289 | rep35 | 658 / 672 | 84.80 | AF295100 |
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| FLHEMEON_1 | 0.0 | 1.0 | 0.0 |
| FLHEMEON_2 | 0.0 | 1.0 | 0.0 |
| FLHEMEON_3 | 0.0 | 1.0 | 0.0 |
| FLHEMEON_4 | 0.0 | 1.0 | 0.0 |
| FLHEMEON_5 | 0.0 | 1.0 | 0.0 |
| FLHEMEON_6 | 0.0 | 1.0 | 0.0 |
| FLHEMEON_7 | 0.0 | 1.0 | 0.0 |
| FLHEMEON_8 | 0.0 | 1.0 | 0.0 |
| FLHEMEON_9 | 0.0 | 1.0 | 0.0 |
| FLHEMEON_10 | 1.0 | 0.0 | 1.0 |
| FLHEMEON_11 | 0.0 | 1.0 | 0.0 |
| FLHEMEON_13 | 1.0 | 0.0 | 1.0 |
| FLHEMEON_21 | 0.0 | 1.0 | 0.0 |
| FLHEMEON_30 | 0.0 | 1.0 | 0.0 |
| FLHEMEON_33 | 0.0 | 1.0 | 0.0 |
| FLHEMEON_38 | 0.0 | 1.0 | 0.0 |
| FLHEMEON_42 | 0.0 | 1.0 | 0.0 |
| FLHEMEON_43 | 0.0 | 1.0 | 0.0 |
| FLHEMEON_44 | 0.0 | 1.0 | 0.0 |
| FLHEMEON_45 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| No hit found | |||||
Phigaro v2.4.0 (Database: Jan. 2024 release)