Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 0 | 16 | 36 | 39.40 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| No hit found | |||||||
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| KNEBMNHP_32 | 1762 | 2493 | STER_RS05315 | 0.0 | Capsule | Immune modulation | 94.94 | 100.00 | WP_011681176 |
| KNEBMNHP_32 | 1762 | 2493 | STU_RS14645 | 0.0 | Capsule | Immune modulation | 94.67 | 100.00 | WP_011226048 |
| KNEBMNHP_35 | 65870 | 66586 | STER_RS07085 | 0.0 | Capsule | Immune modulation | 94.42 | 100.00 | WP_011681442 |
| KNEBMNHP_35 | 62294 | 63145 | rfbD | 0.0 | Capsule | Immune modulation | 94.37 | 100.00 | WP_014621820 |
| KNEBMNHP_32 | 1762 | 2493 | STR_RS05260 | 0.0 | Capsule | Immune modulation | 94.26 | 100.00 | WP_011227248 |
| KNEBMNHP_35 | 66932 | 67702 | STER_RS07075 | 0.0 | Capsule | Immune modulation | 92.35 | 99.23 | WP_011681440 |
| KNEBMNHP_8 | 3024 | 3785 | srtA | 0.0 | Sortase A | Exoenzyme | 91.73 | 100.00 | - |
| KNEBMNHP_32 | 301 | 1761 | STER_RS05320 | 0.0 | Capsule | Immune modulation | 91.40 | 99.86 | WP_011681177 |
| KNEBMNHP_32 | 104836 | 106494 | fbp54 | 0.0 | FBPs | Adherence | 91.14 | 99.94 | WP_011227179 |
| KNEBMNHP_34 | 127097 | 127690 | STR_RS05880 | 0.0 | Capsule | Immune modulation | 91.08 | 100.00 | WP_011226149 |
| KNEBMNHP_32 | 301 | 1761 | STU_RS14650 | 0.0 | Capsule | Immune modulation | 90.92 | 100.00 | WP_011226049 |
| KNEBMNHP_34 | 127097 | 127690 | STER_RS06030 | 0.0 | Capsule | Immune modulation | 90.91 | 100.00 | WP_011226149 |
| KNEBMNHP_34 | 127097 | 127690 | STU_RS15260 | 0.0 | Capsule | Immune modulation | 90.91 | 100.00 | WP_011226149 |
| KNEBMNHP_32 | 301 | 1761 | STR_RS05265 | 0.0 | Capsule | Immune modulation | 90.10 | 100.00 | WP_041827043 |
| KNEBMNHP_32 | 2502 | 3194 | STU_RS14640 | 0.0 | Capsule | Immune modulation | 89.48 | 99.86 | WP_011226047 |
| KNEBMNHP_32 | 2502 | 3194 | STR_RS05255 | 0.0 | Capsule | Immune modulation | 89.19 | 99.86 | WP_011227247 |
| KNEBMNHP_32 | 2502 | 3194 | STER_RS05310 | 0.0 | Capsule | Immune modulation | 89.05 | 99.86 | WP_011681175 |
| KNEBMNHP_6 | 45534 | 46837 | eno | 0.0 | Streptococcal enolase | Exoenzyme | 88.24 | 99.54 | WP_000022832 |
| KNEBMNHP_2 | 71994 | 72833 | cbpD | 0.0 | Autolysin | Exoenzyme | 87.87 | 99.88 | WP_011226733 |
| KNEBMNHP_12 | 13399 | 14412 | plr/gapA | 0.0 | Streptococcal plasmin receptor/GAPDH | Adherence | 85.67 | 99.90 | WP_002986042 |
| KNEBMNHP_34 | 127960 | 129006 | rfbB | 0.0 | Capsule | Immune modulation | 83.59 | 99.90 | WP_002263087 |
| KNEBMNHP_33 | 1763 | 3046 | tig/ropA | 0.0 | Trigger factor | Stress survival | 80.53 | 99.61 | WP_000107753 |
| KNEBMNHP_34 | 126228 | 127097 | rfbA | 0.0 | Capsule | Immune modulation | 80.28 | 99.77 | WP_002904719 |
| KNEBMNHP_32 | 3204 | 3874 | STU_RS14635 | 3.02e-146 | Capsule | Immune modulation | 80.83 | 90.28 | WP_011226046 |
| KNEBMNHP_32 | 3204 | 3874 | STER_RS05305 | 1.40e-144 | Capsule | Immune modulation | 80.68 | 90.28 | WP_011681174 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| KNEBMNHP_8 | 6475 | 7458 | ldh(Sp_1220) | 0.0 | L-lactate dehydrogenase | 96.95 | 100.00 | AAK75326 |
| KNEBMNHP_38 | 11242 | 12315 | MntE | 0.0 | manganese homeostasis | 96.09 | 99.72 | ABF32167 |
| KNEBMNHP_37 | 86939 | 84633 | pfl2(Sp_0459) | 0.0 | formate acetyltransferase | 94.54 | 99.22 | AAK74619 |
| KNEBMNHP_39 | 88766 | 87798 | spr0084 | 0.0 | pathogenitcity related | 93.19 | 98.48 | AAK98888 |
| KNEBMNHP_36 | 83083 | 81602 | SPD_0310 | 0.0 | UPF0371 protein SPD_0310 | 93.12 | 100.00 | ABJ54480 |
| KNEBMNHP_38 | 30299 | 32545 | metE | 0.0 | methionine biosynthesis enzymes | 93.06 | 100.00 | AAK74738 |
| KNEBMNHP_21 | 1050 | 79 | prsA2 | 0.0 | ribose-phosphate pyrophosphokinase | 91.67 | 100.00 | ABF36968 |
| KNEBMNHP_36 | 172432 | 178905 | PrtA | 0.0 | serine protease | 91.39 | 100.61 | ABJ55268 |
| KNEBMNHP_34 | 100852 | 102582 | ptsI | 0.0 | phosphoenolpyruvate-protein phosphotransferase | 89.95 | 100.00 | AAK34199 |
| KNEBMNHP_5 | 24105 | 22954 | potA | 0.0 | ABC transporter ATP-binding protein-spermidine/putrescine transport | 89.58 | 99.74 | AAL00050 |
| KNEBMNHP_35 | 48603 | 50591 | uvrB | 0.0 | excinuclease ABC, B subunit | 89.29 | 100.00 | AE014259_2 |
| KNEBMNHP_4 | 25708 | 27186 | IMPDH | 0.0 | inosine-5'-monophosphate dehydrogenase | 87.83 | 100.00 | ADE32434 |
| KNEBMNHP_6 | 46837 | 45536 | Eno | 0.0 | enolase | 86.44 | 99.77 | ABP90468 |
| KNEBMNHP_4 | 41054 | 42946 | gidA | 0.0 | tRNA uridine 5-carboxymethylaminomethyl modification enzyme | 85.58 | 98.90 | ABP91129 |
| KNEBMNHP_11 | 21716 | 22807 | recA | 0.0 | DNA recombination and repair | 84.64 | 95.36 | AAL00560 |
| KNEBMNHP_38 | 35467 | 33758 | PGM | 0.0 | phosphoglucomutase | 84.59 | 99.65 | ACH87041 |
| KNEBMNHP_1 | 29579 | 30589 | pdh | 0.0 | pyruvate dehydrogenase | 83.38 | 99.70 | ABP91006 |
| KNEBMNHP_32 | 90909 | 92270 | mnmE | 0.0 | central tRNA-modifying GTPase | 82.89 | 99.56 | ABP90420 |
| KNEBMNHP_39 | 13801 | 12911 | bglB | 1.85e-178 | putative phospho--glucosidase | 87.88 | 99.66 | AAK33487 |
| KNEBMNHP_38 | 32735 | 33592 | metF | 1.79e-169 | methionine biosynthesis enzymes | 87.41 | 99.31 | AAK74739 |
| KNEBMNHP_38 | 75416 | 74478 | SP_0676 | 1.04e-168 | transcription facot | 81.47 | 97.20 | AAK74821 |
| KNEBMNHP_34 | 62570 | 61713 | mtsA | 1.87e-151 | substrate-binding lipoprotein of manganese transporter | 80.14 | 92.86 | AIK76191 |
| KNEBMNHP_35 | 62294 | 63115 | GacA | 3.72e-147 | dTDP-4-dehydrorhamnose reductas | 80.29 | 96.48 | AAZ51220 |
| KNEBMNHP_18 | 5086 | 5790 | covR | 3.81e-128 | responder protein | 85.53 | 100.00 | AAK71319 |
| KNEBMNHP_36 | 154461 | 155132 | rr01 | 1.86e-126 | response regulator | 84.38 | 99.56 | CAB54566 |
| KNEBMNHP_38 | 40132 | 40803 | ciaR | 1.92e-125 | two component system response regulator | 86.16 | 99.12 | AIK75708 |
| KNEBMNHP_38 | 40132 | 40800 | CiaR | 3.51e-125 | two-component response regulator | 87.44 | 99.55 | AAK74935 |
| KNEBMNHP_18 | 5086 | 5790 | walR | 8.65e-122 | DNA-binding response regulator | 82.13 | 100.00 | ANC99504 |
| KNEBMNHP_33 | 168122 | 168808 | CovR | 1.86e-120 | serves to repress GAS virulence factor-encoding genes | 82.10 | 100.00 | ACE75886 |
| KNEBMNHP_11 | 22980 | 23375 | SpxA2 | 2.30e-76 | transcriptional regulator | 91.67 | 100.00 | ERL21461 |
| KNEBMNHP_32 | 79442 | 79041 | SpxA1 | 6.98e-70 | transcriptional regulator | 82.09 | 100.00 | ERL19273 |
| KNEBMNHP_3 | 72722 | 73135 | GrpE | 6.10e-68 | protein grpE | 81.88 | 80.59 | ABP89267 |
| KNEBMNHP_33 | 191327 | 191614 | spr0479 | 5.87e-48 | essential gene | 85.42 | 98.97 | AAK99283 |
| KNEBMNHP_39 | 128228 | 127974 | spr1327 | 4.70e-46 | essential gene | 94.12 | 100.00 | AAL00131 |
| KNEBMNHP_7 | 27352 | 27089 | spr0175 | 2.06e-43 | pathogenitcity related | 85.23 | 100.00 | AAK98979 |
| KNEBMNHP_7 | 26527 | 26261 | spr0177 | 4.56e-42 | essential gene | 83.15 | 88.12 | AAK98981 |
| KNEBMNHP_33 | 3213 | 3704 | RopE (M5005_Spy_1611) | 1.07e-72 | probable DNA-directed RNA polymerase subunit delta | 82.93 | 85.86 | AAK34603 |
| KNEBMNHP_37 | 86939 | 84639 | PFL (B9H01_RS01125) | 0.0 | formate C-acetyltransferase | 83.36 | 98.08 | MYN69560 |
| KNEBMNHP_1 | 65712 | 64693 | ilvC (SPD_0406) | 0.0 | ketol-acid reductoisomerase (NADP(+)) | 85.88 | 100.00 | ABJ55451 |
| KNEBMNHP_38 | 142812 | 143351 | pnuC | 3.97e-100 | NAD salvage pathway importer | 89.44 | 66.91 | AAK75931 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| KNEBMNHP_16 | 11657 | 12738 | rep31 | 1084 / 1158 | 87.08 | CP001755 |
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| KNEBMNHP_1 | 0.0 | 1.0 | 0.0 |
| KNEBMNHP_2 | 0.0 | 1.0 | 0.0 |
| KNEBMNHP_3 | 0.0 | 1.0 | 0.0 |
| KNEBMNHP_4 | 0.0 | 1.0 | 0.0 |
| KNEBMNHP_5 | 0.0 | 1.0 | 0.0 |
| KNEBMNHP_6 | 0.0 | 1.0 | 0.0 |
| KNEBMNHP_7 | 0.0 | 1.0 | 0.0 |
| KNEBMNHP_8 | 0.0 | 1.0 | 0.0 |
| KNEBMNHP_9 | 0.0 | 1.0 | 0.0 |
| KNEBMNHP_10 | 0.0 | 1.0 | 0.0 |
| KNEBMNHP_11 | 0.0 | 1.0 | 0.0 |
| KNEBMNHP_12 | 0.0 | 1.0 | 0.0 |
| KNEBMNHP_13 | 0.0 | 1.0 | 0.0 |
| KNEBMNHP_15 | 1.0 | 0.0 | 1.0 |
| KNEBMNHP_16 | 1.0 | 0.0 | 1.0 |
| KNEBMNHP_17 | 0.0 | 1.0 | 0.0 |
| KNEBMNHP_18 | 0.0 | 1.0 | 0.0 |
| KNEBMNHP_19 | 0.0 | 1.0 | 0.0 |
| KNEBMNHP_21 | 0.0 | 1.0 | 0.0 |
| KNEBMNHP_23 | 1.0 | 0.0 | 1.0 |
| KNEBMNHP_24 | 0.0 | 1.0 | 0.0 |
| KNEBMNHP_25 | 0.0 | 1.0 | 0.0 |
| KNEBMNHP_26 | 1.0 | 0.0 | 1.0 |
| KNEBMNHP_32 | 0.0 | 1.0 | 0.0 |
| KNEBMNHP_33 | 0.0 | 1.0 | 0.0 |
| KNEBMNHP_34 | 0.0 | 1.0 | 0.0 |
| KNEBMNHP_35 | 0.0 | 1.0 | 0.0 |
| KNEBMNHP_36 | 0.0 | 1.0 | 0.0 |
| KNEBMNHP_37 | 0.0 | 1.0 | 0.0 |
| KNEBMNHP_38 | 0.0 | 1.0 | 0.0 |
| KNEBMNHP_39 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| No hit found | |||||
Phigaro v2.4.0 (Database: Jan. 2024 release)