Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 2 | 17 | 34 | 38.07 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| FLPIENJJ_57 | 17746 | 18963 | mef(A) | Erythromycin, Azithromycin | 100.00 | 100.00 | U83667 |
| FLPIENJJ_57 | 16163 | 17626 | msr(D) | Erythromycin, Azithromycin, Telithromycin, Quinupristin, Pristinamycin IA, Virginiamycin S | 100.00 | 100.00 | AF274302 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| FLPIENJJ_57 | 17749 | 18963 | mef(A) | macrolide efflux MFS transporter Mef(A) | EXACTX | 100.00 | 100.00 | WP_000417519.1 |
| FLPIENJJ_57 | 16166 | 17626 | msr(D) | ABC-F type ribosomal protection protein Msr(D) | EXACTX | 100.00 | 100.00 | WP_000420313.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| FLPIENJJ_55 | 165148 | 165999 | rfbD | 0.0 | Capsule | Immune modulation | 95.07 | 100.00 | WP_014621820 |
| FLPIENJJ_58 | 1946 | 2677 | STER_RS05315 | 0.0 | Capsule | Immune modulation | 94.81 | 100.00 | WP_011681176 |
| FLPIENJJ_55 | 168423 | 169138 | STER_RS07085 | 0.0 | Capsule | Immune modulation | 94.55 | 99.86 | WP_011681442 |
| FLPIENJJ_58 | 1946 | 2677 | STU_RS14645 | 0.0 | Capsule | Immune modulation | 94.54 | 100.00 | WP_011226048 |
| FLPIENJJ_58 | 1946 | 2677 | STR_RS05260 | 0.0 | Capsule | Immune modulation | 94.13 | 100.00 | WP_011227248 |
| FLPIENJJ_55 | 169484 | 170254 | STER_RS07075 | 0.0 | Capsule | Immune modulation | 92.48 | 99.23 | WP_011681440 |
| FLPIENJJ_55 | 166118 | 167376 | STER_RS07100 | 0.0 | Capsule | Immune modulation | 92.22 | 99.92 | WP_011681445 |
| FLPIENJJ_1 | 92221 | 92814 | STR_RS05880 | 0.0 | Capsule | Immune modulation | 91.25 | 100.00 | WP_011226149 |
| FLPIENJJ_1 | 92221 | 92814 | STER_RS06030 | 0.0 | Capsule | Immune modulation | 91.08 | 100.00 | WP_011226149 |
| FLPIENJJ_1 | 92221 | 92814 | STU_RS15260 | 0.0 | Capsule | Immune modulation | 91.08 | 100.00 | WP_011226149 |
| FLPIENJJ_58 | 485 | 1945 | STER_RS05320 | 0.0 | Capsule | Immune modulation | 90.92 | 99.86 | WP_011681177 |
| FLPIENJJ_58 | 485 | 1945 | STU_RS14650 | 0.0 | Capsule | Immune modulation | 90.78 | 100.00 | WP_011226049 |
| FLPIENJJ_58 | 118127 | 119784 | fbp54 | 0.0 | FBPs | Adherence | 90.36 | 99.88 | WP_011227179 |
| FLPIENJJ_58 | 485 | 1945 | STR_RS05265 | 0.0 | Capsule | Immune modulation | 89.97 | 100.00 | WP_041827043 |
| FLPIENJJ_58 | 2686 | 3378 | STU_RS14640 | 0.0 | Capsule | Immune modulation | 89.90 | 100.00 | WP_011226047 |
| FLPIENJJ_58 | 2686 | 3378 | STR_RS05255 | 0.0 | Capsule | Immune modulation | 89.61 | 100.00 | WP_011227247 |
| FLPIENJJ_58 | 2686 | 3378 | STER_RS05310 | 0.0 | Capsule | Immune modulation | 89.47 | 100.00 | WP_011681175 |
| FLPIENJJ_57 | 55431 | 56734 | eno | 0.0 | Streptococcal enolase | Exoenzyme | 88.24 | 99.54 | WP_000022832 |
| FLPIENJJ_55 | 72390 | 73229 | cbpD | 0.0 | Autolysin | Exoenzyme | 87.87 | 99.88 | WP_011226733 |
| FLPIENJJ_14 | 13424 | 14437 | plr/gapA | 0.0 | Streptococcal plasmin receptor/GAPDH | Adherence | 85.67 | 99.90 | WP_002986042 |
| FLPIENJJ_44 | 37976 | 38737 | srtA | 0.0 | Sortase A | Exoenzyme | 84.40 | 99.87 | - |
| FLPIENJJ_1 | 90905 | 91951 | rfbB | 0.0 | Capsule | Immune modulation | 83.40 | 99.90 | WP_002263087 |
| FLPIENJJ_6 | 47364 | 48647 | tig/ropA | 0.0 | Trigger factor | Stress survival | 80.84 | 99.61 | WP_000107753 |
| FLPIENJJ_1 | 92814 | 93683 | rfbA | 0.0 | Capsule | Immune modulation | 80.39 | 99.77 | WP_002904719 |
| FLPIENJJ_58 | 3388 | 4058 | STU_RS14635 | 7.27e-148 | Capsule | Immune modulation | 80.98 | 90.28 | WP_011226046 |
| FLPIENJJ_58 | 3388 | 4058 | STER_RS05305 | 3.38e-146 | Capsule | Immune modulation | 80.83 | 90.28 | WP_011681174 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| FLPIENJJ_44 | 35287 | 34304 | ldh(Sp_1220) | 0.0 | L-lactate dehydrogenase | 96.95 | 100.00 | AAK75326 |
| FLPIENJJ_44 | 46873 | 47946 | MntE | 0.0 | manganese homeostasis | 96.09 | 99.72 | ABF32167 |
| FLPIENJJ_11 | 18374 | 16068 | pfl2(Sp_0459) | 0.0 | formate acetyltransferase | 94.41 | 99.22 | AAK74619 |
| FLPIENJJ_44 | 66413 | 68659 | metE | 0.0 | methionine biosynthesis enzymes | 92.92 | 100.00 | AAK74738 |
| FLPIENJJ_51 | 95501 | 96982 | SPD_0310 | 0.0 | UPF0371 protein SPD_0310 | 92.92 | 100.00 | ABJ54480 |
| FLPIENJJ_11 | 229484 | 230452 | spr0084 | 0.0 | pathogenitcity related | 92.26 | 98.48 | AAK98888 |
| FLPIENJJ_19 | 1079 | 108 | prsA2 | 0.0 | ribose-phosphate pyrophosphokinase | 91.67 | 100.00 | ABF36968 |
| FLPIENJJ_15 | 9592 | 11322 | ptsI | 0.0 | phosphoenolpyruvate-protein phosphotransferase | 89.95 | 100.00 | AAK34199 |
| FLPIENJJ_55 | 150014 | 152002 | uvrB | 0.0 | excinuclease ABC, B subunit | 89.74 | 100.00 | AE014259_2 |
| FLPIENJJ_11 | 153334 | 154485 | potA | 0.0 | ABC transporter ATP-binding protein-spermidine/putrescine transport | 89.58 | 99.74 | AAL00050 |
| FLPIENJJ_22 | 7293 | 8771 | IMPDH | 0.0 | inosine-5'-monophosphate dehydrogenase | 87.83 | 100.00 | ADE32434 |
| FLPIENJJ_57 | 56734 | 55433 | Eno | 0.0 | enolase | 86.44 | 99.77 | ABP90468 |
| FLPIENJJ_22 | 23073 | 24965 | gidA | 0.0 | tRNA uridine 5-carboxymethylaminomethyl modification enzyme | 85.58 | 98.90 | ABP91129 |
| FLPIENJJ_9 | 1213 | 122 | recA | 0.0 | DNA recombination and repair | 84.91 | 95.36 | AAL00560 |
| FLPIENJJ_44 | 71577 | 69868 | PGM | 0.0 | phosphoglucomutase | 84.76 | 99.65 | ACH87041 |
| FLPIENJJ_58 | 104365 | 105726 | mnmE | 0.0 | central tRNA-modifying GTPase | 82.89 | 99.56 | ABP90420 |
| FLPIENJJ_22 | 178604 | 177594 | pdh | 0.0 | pyruvate dehydrogenase | 82.79 | 99.70 | ABP91006 |
| FLPIENJJ_57 | 71144 | 70254 | bglB | 1.47e-177 | putative phospho--glucosidase | 87.20 | 99.66 | AAK33487 |
| FLPIENJJ_44 | 68849 | 69706 | metF | 6.26e-170 | methionine biosynthesis enzymes | 87.76 | 99.31 | AAK74739 |
| FLPIENJJ_44 | 113114 | 112176 | SP_0676 | 1.29e-168 | transcription facot | 81.47 | 97.20 | AAK74821 |
| FLPIENJJ_3 | 27439 | 28296 | mtsA | 1.14e-151 | substrate-binding lipoprotein of manganese transporter | 80.14 | 92.86 | AIK76191 |
| FLPIENJJ_10 | 2303 | 3007 | covR | 1.52e-127 | responder protein | 85.53 | 100.00 | AAK71319 |
| FLPIENJJ_51 | 29974 | 29303 | rr01 | 1.54e-126 | response regulator | 84.82 | 99.56 | CAB54566 |
| FLPIENJJ_44 | 76242 | 76913 | ciaR | 1.81e-125 | two component system response regulator | 86.16 | 99.12 | AIK75708 |
| FLPIENJJ_44 | 76242 | 76910 | CiaR | 3.35e-125 | two-component response regulator | 87.44 | 99.55 | AAK74935 |
| FLPIENJJ_10 | 2303 | 3007 | walR | 3.45e-121 | DNA-binding response regulator | 82.13 | 100.00 | ANC99504 |
| FLPIENJJ_2 | 36470 | 35784 | CovR | 8.73e-121 | serves to repress GAS virulence factor-encoding genes | 82.10 | 100.00 | ACE75886 |
| FLPIENJJ_28 | 204 | 599 | SpxA2 | 3.56e-85 | transcriptional regulator | 91.67 | 100.00 | ERL21461 |
| FLPIENJJ_58 | 92907 | 92506 | SpxA1 | 7.82e-70 | transcriptional regulator | 82.09 | 100.00 | ERL19273 |
| FLPIENJJ_8 | 6424 | 6011 | GrpE | 3.06e-68 | protein grpE | 81.88 | 80.59 | ABP89267 |
| FLPIENJJ_2 | 13265 | 12978 | spr0479 | 5.71e-48 | essential gene | 85.42 | 98.97 | AAK99283 |
| FLPIENJJ_11 | 195817 | 196071 | spr1327 | 7.57e-46 | essential gene | 94.12 | 100.00 | AAL00131 |
| FLPIENJJ_22 | 87929 | 87666 | spr0175 | 1.08e-42 | pathogenitcity related | 85.23 | 100.00 | AAK98979 |
| FLPIENJJ_22 | 87104 | 86838 | spr0177 | 2.39e-41 | essential gene | 83.15 | 88.12 | AAK98981 |
| FLPIENJJ_6 | 47197 | 46706 | RopE (M5005_Spy_1611) | 2.43e-73 | probable DNA-directed RNA polymerase subunit delta | 83.54 | 85.86 | AAK34603 |
| FLPIENJJ_11 | 18374 | 16074 | PFL (B9H01_RS01125) | 0.0 | formate C-acetyltransferase | 83.22 | 98.08 | MYN69560 |
| FLPIENJJ_22 | 142190 | 143209 | ilvC (SPD_0406) | 0.0 | ketol-acid reductoisomerase (NADP(+)) | 85.88 | 100.00 | ABJ55451 |
| FLPIENJJ_44 | 179660 | 180199 | pnuC | 1.06e-92 | NAD salvage pathway importer | 82.78 | 66.91 | AAK75931 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| FLPIENJJ_1 | 0.0 | 1.0 | 0.0 |
| FLPIENJJ_2 | 0.0 | 1.0 | 0.0 |
| FLPIENJJ_3 | 0.0 | 1.0 | 0.0 |
| FLPIENJJ_4 | 0.0 | 1.0 | 0.0 |
| FLPIENJJ_5 | 0.0 | 1.0 | 0.0 |
| FLPIENJJ_6 | 0.0 | 1.0 | 0.0 |
| FLPIENJJ_7 | 0.0 | 1.0 | 0.0 |
| FLPIENJJ_8 | 0.0 | 1.0 | 0.0 |
| FLPIENJJ_9 | 0.0 | 1.0 | 0.0 |
| FLPIENJJ_10 | 0.0 | 1.0 | 0.0 |
| FLPIENJJ_11 | 0.0 | 1.0 | 0.0 |
| FLPIENJJ_12 | 0.0 | 1.0 | 0.0 |
| FLPIENJJ_13 | 0.0 | 1.0 | 0.0 |
| FLPIENJJ_14 | 0.0 | 1.0 | 0.0 |
| FLPIENJJ_15 | 0.0 | 1.0 | 0.0 |
| FLPIENJJ_17 | 0.0 | 1.0 | 0.0 |
| FLPIENJJ_18 | 0.0 | 1.0 | 0.0 |
| FLPIENJJ_19 | 0.0 | 1.0 | 0.0 |
| FLPIENJJ_21 | 1.0 | 0.0 | 1.0 |
| FLPIENJJ_22 | 0.0 | 1.0 | 0.0 |
| FLPIENJJ_23 | 0.0 | 1.0 | 0.0 |
| FLPIENJJ_24 | 1.0 | 0.0 | 1.0 |
| FLPIENJJ_25 | 0.0 | 1.0 | 0.0 |
| FLPIENJJ_26 | 0.0 | 1.0 | 0.0 |
| FLPIENJJ_33 | 1.0 | 0.0 | 1.0 |
| FLPIENJJ_44 | 0.0 | 1.0 | 0.0 |
| FLPIENJJ_51 | 0.0 | 1.0 | 0.0 |
| FLPIENJJ_55 | 0.0 | 1.0 | 0.0 |
| FLPIENJJ_56 | 0.0 | 1.0 | 0.0 |
| FLPIENJJ_57 | 0.0 | 1.0 | 0.0 |
| FLPIENJJ_58 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| No hit found | |||||
Phigaro v2.4.0 (Database: Jan. 2024 release)