Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 11 | 10 | 4 | 15.39 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| HLLBMAHE_1 | 2642828 | 2644030 | blt | ARO:3003006 | blt | 99.25 | 100.00 | L32599.1 |
| HLLBMAHE_1 | 2469568 | 2470737 | bmr | ARO:3003007 | bmr | 99.74 | 100.00 | M33768.1 |
| HLLBMAHE_1 | 1447639 | 1447956 | ykkD | ARO:3003064 | ykkD | 99.05 | 100.00 | AL009126.1 |
| HLLBMAHE_1 | 1447301 | 1447639 | ykkC | ARO:3003063 | ykkC | 100.00 | 100.00 | AL009126.1 |
| HLLBMAHE_1 | 960795 | 963365 | Bacillus subtilis mprF | ARO:3003324 | Bsub_mprF | 99.77 | 100.00 | AL009126.3 |
| HLLBMAHE_1 | 586380 | 588026 | vmlR | ARO:3004476 | vmlR | 98.18 | 100.18 | NC_000964.3 |
| HLLBMAHE_1 | 340959 | 341552 | tmrB | ARO:3003059 | tmrB | 97.97 | 100.00 | AL009126.3 |
| HLLBMAHE_1 | 290259 | 291692 | lmrB | ARO:3002813 | lmrB | 98.95 | 100.00 | JYFL01000006.1 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| HLLBMAHE_1 | 277447 | 278366 | mph(K) | Spiramycin, Telithromycin | 97.72 | 99.89 | NC_000964 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| HLLBMAHE_1 | 586383 | 588026 | vmlR | ABC-F type ribosomal protection protein VmlR | BLASTX | 98.18 | 100.00 | WP_003234144.1 |
| HLLBMAHE_1 | 4170605 | 4171123 | satA | streptothricin N-acetyltransferase SatA | BLASTX | 91.33 | 100.00 | WP_003242546.1 |
| HLLBMAHE_1 | 2034634 | 2037231 | rphC | rifamycin-inactivating phosphotransferase RphC | BLASTX | 80.94 | 100.00 | WP_087347987.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| HLLBMAHE_1 | 3678587 | 3679036 | capC | 0.0 | Capsule | Immune modulation | 99.56 | 100.00 | NP_391470 |
| HLLBMAHE_1 | 3679051 | 3680232 | capB | 0.0 | Capsule | Immune modulation | 99.49 | 100.00 | NP_391471 |
| HLLBMAHE_1 | 2266528 | 2267169 | hlyIII | 0.0 | Hemolysin III | Exotoxin | 99.38 | 100.00 | NP_390062 |
| HLLBMAHE_1 | 3192259 | 3193044 | dhbA | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.36 | 100.00 | NP_391080 |
| HLLBMAHE_1 | 3677426 | 3678568 | capA | 0.0 | Capsule | Immune modulation | 99.30 | 100.00 | NP_391469 |
| HLLBMAHE_1 | 1988761 | 1990524 | dep/capD | 0.0 | Capsule | Immune modulation | 98.98 | 100.00 | NP_389723 |
| HLLBMAHE_1 | 3188434 | 3189372 | dhbB | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.94 | 100.00 | NP_391077 |
| HLLBMAHE_1 | 3191037 | 3192233 | dhbC | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.75 | 100.00 | NP_391079 |
| HLLBMAHE_1 | 3181278 | 3188414 | dhbF | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.57 | 100.00 | NP_391076 |
| HLLBMAHE_1 | 3189400 | 3191019 | dhbE | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 97.28 | 100.00 | NP_391078 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| HLLBMAHE_1 | 132497 | 133633 | tufA | 0.0 | elongation factor Tu | 85.75 | 95.69 | AHM69299 |
| HLLBMAHE_1 | 18601 | 19470 | pdxS | 3.12e-149 | encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS | 83.79 | 98.31 | ACE61268 |
| HLLBMAHE_1 | 1757973 | 1758749 | CodY | 3.41e-124 | nutrient-responsive regulator | 81.08 | 100.00 | ACJ80679 |
| HLLBMAHE_1 | 3522101 | 3522670 | ClpP | 2.53e-98 | part of proteolytic complex | 81.05 | 97.44 | BAB94595 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| HLLBMAHE_3 | 1 | 191 | Col(SD853) | 191 / 194 | 97.91 | NC015392 |
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| HLLBMAHE_1 | 0.0 | 1.0 | 0.0 |
| HLLBMAHE_2 | 1.0 | 0.0 | 1.0 |
| HLLBMAHE_3 | 1.0 | 0.0 | 1.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| HLLBMAHE_1 | 651866 | 674460 | False | Siphoviridae | VOG0796, VOG10227, VOG0720, VOG4564, VOG7363, VOG4555, VOG1329, VOG3480, VOG4623, VOG0816, VOG5660, VOG0724, VOG0799, VOG2621, VOG0725, VOG4545, VOG4605, VOG4599, VOG3390, VOG4705, VOG5615, VOG0753, VOG0136, VOG4574 |
| HLLBMAHE_1 | 1391192 | 1416488 | False | Myoviridae | VOG4602, VOG9667, VOG3490, VOG0189, VOG4861, VOG5447, VOG0796, VOG10227, VOG4773, VOG4685, VOG0749, VOG3890, VOG3891, VOG3892, VOG6163, VOG4865, VOG4885, VOG3894, VOG4550, VOG4691, VOG4862, VOG7718, VOG3390, VOG7895, VOG0753 |
| HLLBMAHE_1 | 2205009 | 2210690 | False | Myoviridae | VOG5722, VOG5779, VOG5721 |
| HLLBMAHE_1 | 2222693 | 2240309 | False | Siphoviridae | VOG9312, VOG6163, VOG4910, VOG3389, VOG5042, VOG9712, VOG4626, VOG3390, VOG4705, VOG4574, VOG8537 |
| HLLBMAHE_1 | 3373682 | 3397614 | False | Siphoviridae | VOG4574, VOG0641, VOG4705, VOG3390, VOG4612, VOG4705, VOG3390, VOG4626, VOG4772, VOG0801, VOG5910, VOG0541, VOG4586, VOG0704, VOG9583, VOG0204, VOG5361, VOG4553, VOG4568, VOG4556, VOG3607, VOG1886, VOG4581, VOG0713, VOG4841 |
| HLLBMAHE_1 | 3405500 | 3409181 | False | Siphoviridae | VOG9667, VOG9667, VOG1563 |
Phigaro v2.4.0 (Database: Jan. 2024 release)