safety

PPRS ARGs VFs PGs Plasmid Prophage IS elements

Probiotic potential risk score

ARGs	VFs	PGs	PPRS
0	34	28	44.05

PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).

Antibiotic resistance genes

Comprehensive Antibiotic Resistance Database (CARD)

Query ID	Begin	End	ARO name	ARO accession	CARD name	Identity	Coverage	Accession no.
No hit found

RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)

ResFinder

Query ID	Begin	End	Resistance gene	Phenotype	Identity	Coverage	Accession no.
No hit found

ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)

AMRFinderPlus

Query ID	Begin	End	Gene symbol	Sequence name	Method	Identity	Coverage	Accession no.
No hit found

AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)

Virulence factors

Virulence Factor Database (VFDB)

Query ID	qstart	qend	Gene name	e-value	VF name	VF category	Identity	Coverage	Accession no.
APIMJJON_1	43663	44502	cbpD	0.0	Autolysin	Exoenzyme	100.00	100.00	WP_011226733
APIMJJON_1	1038335	1039125	cshA	0.0	Cell surface hydrophobicity proteins	Adherence	100.00	100.00	-
APIMJJON_1	1308822	1309673	rfbD	0.0	Capsule	Immune modulation	100.00	100.00	WP_014621820
APIMJJON_1	1119748	1120509	srtA	0.0	Sortase A	Exoenzyme	100.00	100.00	-
APIMJJON_1	962124	963047	STR_RS05205	0.0	Capsule	Immune modulation	100.00	100.00	WP_011227237
APIMJJON_1	963007	963957	STR_RS05210	0.0	Capsule	Immune modulation	100.00	100.00	WP_011227238
APIMJJON_1	963982	965064	STR_RS05215	0.0	Capsule	Immune modulation	100.00	100.00	WP_011227239
APIMJJON_1	965154	966128	STR_RS05220	0.0	Capsule	Immune modulation	100.00	100.00	WP_011227240
APIMJJON_1	966134	966613	STR_RS05225	0.0	Capsule	Immune modulation	100.00	100.00	WP_041827041
APIMJJON_1	966806	967924	STR_RS05230	0.0	Capsule	Immune modulation	100.00	100.00	WP_148148887
APIMJJON_1	968492	969451	STR_RS05240	0.0	Capsule	Immune modulation	100.00	100.00	WP_041827042
APIMJJON_1	969615	970214	STR_RS05245	0.0	Capsule	Immune modulation	100.00	100.00	WP_196767671
APIMJJON_1	970351	971100	STR_RS05250	0.0	Capsule	Immune modulation	100.00	100.00	WP_011227246
APIMJJON_1	971110	971802	STR_RS05255	0.0	Capsule	Immune modulation	100.00	100.00	WP_011227247
APIMJJON_1	971811	972542	STR_RS05260	0.0	Capsule	Immune modulation	100.00	100.00	WP_011227248
APIMJJON_1	972543	973997	STR_RS05265	0.0	Capsule	Immune modulation	100.00	100.00	WP_041827043
APIMJJON_1	1084391	1084984	STR_RS05880	0.0	Capsule	Immune modulation	100.00	100.00	WP_011226149
APIMJJON_1	1296344	1298089	STR_RS06885	0.0	Capsule	Immune modulation	100.00	100.00	WP_011227426
APIMJJON_1	1298091	1299791	STR_RS06890	0.0	Capsule	Immune modulation	100.00	100.00	WP_041827077
APIMJJON_1	1299809	1301011	STR_RS06895	0.0	Capsule	Immune modulation	100.00	100.00	WP_011227428
APIMJJON_1	1301011	1301817	STR_RS06900	0.0	Capsule	Immune modulation	100.00	100.00	WP_011227429
APIMJJON_1	1301817	1302758	STR_RS06905	0.0	Capsule	Immune modulation	100.00	100.00	WP_011227430
APIMJJON_1	1302755	1303903	STR_RS06910	0.0	Capsule	Immune modulation	100.00	100.00	WP_011227431
APIMJJON_1	957943	959001	waaB	0.0	Capsule	Immune modulation	100.00	100.00	WP_157822584
APIMJJON_1	856603	858261	fbp54	0.0	FBPs	Adherence	99.94	100.00	WP_011227179
APIMJJON_1	960695	962116	STR_RS05200	0.0	Capsule	Immune modulation	99.93	100.00	WP_011227236
APIMJJON_1	1084391	1084984	STER_RS06030	0.0	Capsule	Immune modulation	99.83	100.00	WP_011226149
APIMJJON_1	1084391	1084984	STU_RS15260	0.0	Capsule	Immune modulation	99.83	100.00	WP_011226149
APIMJJON_1	1298091	1299791	STER_RS07050	0.0	Capsule	Immune modulation	99.71	100.00	WP_041827753
APIMJJON_1	1301011	1301817	STER_RS07060	0.0	Capsule	Immune modulation	99.50	100.00	WP_011681437
APIMJJON_1	1301817	1302758	STER_RS07065	0.0	Capsule	Immune modulation	99.47	100.00	WP_011681438
APIMJJON_1	1299809	1301011	STER_RS07055	0.0	Capsule	Immune modulation	99.42	100.00	WP_011681436
APIMJJON_1	971110	971802	STU_RS14640	0.0	Capsule	Immune modulation	99.42	100.00	WP_011226047
APIMJJON_1	1302755	1303903	STER_RS07070	0.0	Capsule	Immune modulation	99.22	100.00	WP_011681439
APIMJJON_1	1084984	1085853	rfbA	0.0	Capsule	Immune modulation	99.19	100.00	WP_011681286
APIMJJON_1	971811	972542	STU_RS14645	0.0	Capsule	Immune modulation	98.77	100.00	WP_011226048
APIMJJON_1	970365	971100	STER_RS05305	0.0	Capsule	Immune modulation	98.50	99.33	WP_011681174
APIMJJON_1	971811	972542	STER_RS05315	0.0	Capsule	Immune modulation	98.22	100.00	WP_011681176
APIMJJON_1	1296344	1298089	STER_RS07045	0.0	Capsule	Immune modulation	98.22	100.00	WP_011681434
APIMJJON_1	970365	971100	STU_RS14635	0.0	Capsule	Immune modulation	97.96	99.33	WP_011226046
APIMJJON_1	972543	973997	STU_RS14650	0.0	Capsule	Immune modulation	97.94	100.00	WP_011226049
APIMJJON_1	1307442	1308701	STER_RS07100	0.0	Capsule	Immune modulation	97.70	100.00	WP_011681445
APIMJJON_1	972543	973997	STER_RS05320	0.0	Capsule	Immune modulation	96.92	99.79	WP_011681177
APIMJJON_1	971110	971802	STER_RS05310	0.0	Capsule	Immune modulation	89.61	100.00	WP_011681175
APIMJJON_1	589970	591273	eno	0.0	Streptococcal enolase	Exoenzyme	88.09	99.54	WP_000022832
APIMJJON_1	1589397	1590407	plr/gapA	0.0	Streptococcal plasmin receptor/GAPDH	Adherence	86.92	99.51	WP_002986042
APIMJJON_1	1301011	1301817	SGO_RS05020	0.0	Capsule	Immune modulation	82.90	100.00	WP_012000440
APIMJJON_1	143231	144514	tig/ropA	0.0	Trigger factor	Stress survival	80.65	99.77	WP_000107753
APIMJJON_1	1082916	1083962	rfbB	0.0	Capsule	Immune modulation	80.61	100.00	WP_009659423
APIMJJON_1	1306747	1307445	STER_RS07095	2.92e-152	Capsule	Immune modulation	81.15	70.35	WP_011681444
APIMJJON_1	968200	968436	STR_RS05235*	6.59e-119	Capsule	Immune modulation	100.00	100.00	-
APIMJJON_1	1305927	1306393	STER_RS07085	0.0	Capsule	Immune modulation	94.02	65.13	WP_011681442
APIMJJON_1	1300274	1301011	SGO_RS05025	0.0	Capsule	Immune modulation	85.23	60.29	WP_012000441

BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)

VirulenceFinder

Query ID	Begin	End	Database	Virulence factor	Protein function	Identity	Coverage	Accession no.
No hit found

VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)

Pathogenic genes

PHI-base

Query ID	qstart	qend	Gene name	e-value	Function	Identity	Coverage	Gene ID
APIMJJON_1	1123196	1124179	ldh(Sp_1220)	0.0	L-lactate dehydrogenase	94.82	100.00	AAK75326
APIMJJON_1	702345	703418	MntE	0.0	manganese homeostasis	94.41	99.72	ABF32167
APIMJJON_1	507734	508702	spr0084	0.0	pathogenitcity related	93.19	98.48	AAK98888
APIMJJON_1	1471007	1473310	pfl2(Sp_0459)	0.0	formate acetyltransferase	92.97	99.22	AAK74619
APIMJJON_1	1210057	1211538	SPD_0310	0.0	UPF0371 protein SPD_0310	92.11	100.00	ABJ54480
APIMJJON_1	731026	733272	metE	0.0	methionine biosynthesis enzymes	91.99	100.00	AAK74738
APIMJJON_1	63294	64286	recA	0.0	DNA recombination and repair	91.24	85.31	AAL00560
APIMJJON_1	27181	28152	prsA2	0.0	ribose-phosphate pyrophosphokinase	90.12	100.00	ABF36968
APIMJJON_1	1357176	1356025	potA	0.0	ABC transporter ATP-binding protein-spermidine/putrescine transport	89.06	99.74	AAL00050
APIMJJON_1	1322703	1320721	uvrB	0.0	excinuclease ABC, B subunit	88.96	99.70	AE014259_2
APIMJJON_1	1107002	1105272	ptsI	0.0	phosphoenolpyruvate-protein phosphotransferase	87.69	100.00	AAK34199
APIMJJON_1	1778923	1777445	IMPDH	0.0	inosine-5'-monophosphate dehydrogenase	87.63	100.00	ADE32434
APIMJJON_1	589970	591271	Eno	0.0	enolase	86.21	99.77	ABP90468
APIMJJON_1	1764187	1762295	gidA	0.0	tRNA uridine 5-carboxymethylaminomethyl modification enzyme	85.89	98.90	ABP91129
APIMJJON_1	736197	734488	PGM	0.0	phosphoglucomutase	83.89	99.65	ACH87041
APIMJJON_1	1631088	1632098	pdh	0.0	pyruvate dehydrogenase	83.09	99.70	ABP91006
APIMJJON_1	880049	878688	mnmE	0.0	central tRNA-modifying GTPase	82.24	99.56	ABP90420
APIMJJON_1	1681419	1679527	PepO	0.0	endopeptidase	80.03	100.00	ABF32980
APIMJJON_1	575540	576430	bglB	2.81e-178	putative phospho--glucosidase	88.55	99.66	AAK33487
APIMJJON_1	795596	794661	SP_0676	7.13e-168	transcription facot	81.73	96.89	AAK74821
APIMJJON_1	733464	734321	metF	8.35e-165	methionine biosynthesis enzymes	86.36	99.31	AAK74739
APIMJJON_1	1010635	1009931	covR	3.38e-130	responder protein	84.68	100.00	AAK71319
APIMJJON_1	1176378	1175707	rr01	1.16e-125	response regulator	84.38	99.56	CAB54566
APIMJJON_1	1010635	1009931	walR	2.43e-120	DNA-binding response regulator	80.85	100.00	ANC99504
APIMJJON_1	294658	295344	CovR	9.72e-118	serves to repress GAS virulence factor-encoding genes	80.79	100.00	ACE75886
APIMJJON_1	740150	740800	CiaR	1.34e-116	two-component response regulator	86.17	96.88	AAK74935
APIMJJON_1	740150	740800	ciaR	6.88e-114	two component system response regulator	83.87	96.02	AIK75708
APIMJJON_1	64583	64978	SpxA2	3.58e-74	transcriptional regulator	90.91	100.00	ERL21461
APIMJJON_1	144672	145172	RopE (M5005_Spy_1611)	9.54e-75	probable DNA-directed RNA polymerase subunit delta	80.84	87.43	AAK34603
APIMJJON_1	1471007	1473304	PFL (B9H01_RS01125)	0.0	formate C-acetyltransferase	82.94	98.08	MYN69560
APIMJJON_1	1666958	1665939	ilvC (SPD_0406)	0.0	ketol-acid reductoisomerase (NADP(+))	96.18	100.00	ABJ55451

BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)

Plasmid

PlasmidFinder

Query ID	Begin	End	Plasmid	Query / Template	Identity	Accession no.
No hit found

PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)

PlasmidHunter

Query ID	Prediction	Chromosome	Plasmid
APIMJJON_1	0.0	1.0	0.0

PlasmidHunter v 1.4.5 (Database: May. 2024 release)

Prophage

Phigaro

Query ID	Begin	End	Transposable	Taxonomy	pVOGs
No hit found