Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 4 | 1 | 4 | 5.74 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| OBIMPMDI_18 | 238696 | 239466 | ANT(4')-Ib | ARO:3003905 | ANT(4')-Ib | 94.14 | 100.00 | EF540343.1 |
| OBIMPMDI_3 | 133937 | 134782 | Erm(34) | ARO:3000600 | Erm(34) | 98.58 | 100.00 | AY234334.1 |
| OBIMPMDI_22 | 9199 | 10251 | clbC | ARO:3002816 | clbC | 96.57 | 100.00 | AP006627.1 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| OBIMPMDI_3 | 133937 | 134782 | erm(34) | Erythromycin, Lincomycin, Clindamycin, Quinupristin, Pristinamycin IA, Virginiamycin S | 97.87 | 100.00 | AY234334 |
| OBIMPMDI_18 | 238696 | 239466 | ant(4')-Ib | Amikacin, Tobramycin, Isepamicin, Dibekacin | 94.16 | 100.00 | AJ506108 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| OBIMPMDI_3 | 133940 | 134782 | erm(34) | 23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(34) | BLASTX | 98.58 | 100.00 | WP_063844504.1 |
| OBIMPMDI_18 | 241880 | 242782 | blaBCL | BCL family class A beta-lactamase | BLASTX | 96.68 | 100.00 | WP_065825670.1 |
| OBIMPMDI_22 | 9199 | 10248 | clbB | 23S rRNA (adenine(2503)-C(8))-methyltransferase ClbB | BLASTX | 96.57 | 100.00 | WP_011245929.1 |
| OBIMPMDI_18 | 238696 | 239463 | aadD2 | aminoglycoside O-nucleotidyltransferase ANT(4')-Ib | BLASTX | 94.14 | 100.00 | WP_035202393.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| OBIMPMDI_47 | 8567 | 9217 | hlyIII | 0.0 | Hemolysin III | Exotoxin | 96.77 | 100.00 | WP_011247375 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| OBIMPMDI_42 | 37863 | 36721 | tufA | 0.0 | elongation factor Tu | 87.14 | 96.19 | AHM69299 |
| OBIMPMDI_16 | 36506 | 36748 | SpoVG | 2.57e-37 | RNA-binding protein | 81.48 | 79.41 | AAT02994 |
| OBIMPMDI_52 | 35494 | 35297 | CspA | 6.96e-31 | cold shock protein | 86.36 | 100.00 | CAA62903 |
| OBIMPMDI_4 | 49370 | 49182 | CspB | 1.93e-25 | cold shock protein | 82.54 | 95.45 | CAD00094 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| OBIMPMDI_1 | 0.0 | 1.0 | 0.0 |
| OBIMPMDI_2 | 0.0 | 1.0 | 0.0 |
| OBIMPMDI_3 | 0.0 | 1.0 | 0.0 |
| OBIMPMDI_4 | 0.0 | 1.0 | 0.0 |
| OBIMPMDI_5 | 0.0 | 1.0 | 0.0 |
| OBIMPMDI_6 | 0.0 | 1.0 | 0.0 |
| OBIMPMDI_7 | 0.0 | 1.0 | 0.0 |
| OBIMPMDI_8 | 0.0 | 1.0 | 0.0 |
| OBIMPMDI_9 | 0.0 | 1.0 | 0.0 |
| OBIMPMDI_10 | 0.0 | 1.0 | 0.0 |
| OBIMPMDI_11 | 0.0 | 1.0 | 0.0 |
| OBIMPMDI_12 | 0.0 | 1.0 | 0.0 |
| OBIMPMDI_13 | 0.0 | 1.0 | 0.0 |
| OBIMPMDI_14 | 0.0 | 1.0 | 0.0 |
| OBIMPMDI_15 | 0.0 | 1.0 | 0.0 |
| OBIMPMDI_16 | 0.0 | 1.0 | 0.0 |
| OBIMPMDI_17 | 0.0 | 1.0 | 0.0 |
| OBIMPMDI_18 | 0.0 | 1.0 | 0.0 |
| OBIMPMDI_19 | 0.0 | 1.0 | 0.0 |
| OBIMPMDI_20 | 0.0 | 1.0 | 0.0 |
| OBIMPMDI_21 | 0.0 | 1.0 | 0.0 |
| OBIMPMDI_22 | 0.0 | 1.0 | 0.0 |
| OBIMPMDI_23 | 0.0 | 1.0 | 0.0 |
| OBIMPMDI_24 | 0.0 | 1.0 | 0.0 |
| OBIMPMDI_25 | 0.0 | 1.0 | 0.0 |
| OBIMPMDI_26 | 0.0 | 1.0 | 0.0 |
| OBIMPMDI_27 | 0.0 | 1.0 | 0.0 |
| OBIMPMDI_28 | 0.0 | 1.0 | 0.0 |
| OBIMPMDI_29 | 0.0 | 1.0 | 0.0 |
| OBIMPMDI_30 | 0.0 | 1.0 | 0.0 |
| OBIMPMDI_31 | 0.0 | 1.0 | 0.0 |
| OBIMPMDI_32 | 0.0 | 1.0 | 0.0 |
| OBIMPMDI_33 | 0.0 | 1.0 | 0.0 |
| OBIMPMDI_34 | 0.0 | 1.0 | 0.0 |
| OBIMPMDI_35 | 0.0 | 1.0 | 0.0 |
| OBIMPMDI_36 | 0.0 | 1.0 | 0.0 |
| OBIMPMDI_37 | 0.0 | 1.0 | 0.0 |
| OBIMPMDI_38 | 1.0 | 0.0 | 1.0 |
| OBIMPMDI_39 | 0.0 | 1.0 | 0.0 |
| OBIMPMDI_41 | 0.0 | 1.0 | 0.0 |
| OBIMPMDI_42 | 0.0 | 1.0 | 0.0 |
| OBIMPMDI_43 | 0.0 | 1.0 | 0.0 |
| OBIMPMDI_44 | 1.0 | 0.0 | 1.0 |
| OBIMPMDI_45 | 1.0 | 0.0 | 1.0 |
| OBIMPMDI_46 | 1.0 | 0.0 | 1.0 |
| OBIMPMDI_47 | 0.0 | 1.0 | 0.0 |
| OBIMPMDI_48 | 1.0 | 0.0 | 1.0 |
| OBIMPMDI_49 | 0.0 | 1.0 | 0.0 |
| OBIMPMDI_50 | 0.0 | 1.0 | 0.0 |
| OBIMPMDI_51 | 0.0 | 1.0 | 0.0 |
| OBIMPMDI_52 | 0.0 | 1.0 | 0.0 |
| OBIMPMDI_53 | 0.0 | 1.0 | 0.0 |
| OBIMPMDI_54 | 1.0 | 0.0 | 1.0 |
| OBIMPMDI_55 | 0.0 | 1.0 | 0.0 |
| OBIMPMDI_56 | 0.0 | 1.0 | 0.0 |
| OBIMPMDI_57 | 0.0 | 1.0 | 0.0 |
| OBIMPMDI_59 | 0.0 | 1.0 | 0.0 |
| OBIMPMDI_60 | 1.0 | 0.0 | 1.0 |
| OBIMPMDI_62 | 1.0 | 0.0 | 1.0 |
| OBIMPMDI_63 | 0.0 | 1.0 | 0.0 |
| OBIMPMDI_64 | 0.0 | 1.0 | 0.0 |
| OBIMPMDI_66 | 1.0 | 0.0 | 1.0 |
| OBIMPMDI_67 | 1.0 | 0.0 | 1.0 |
| OBIMPMDI_69 | 1.0 | 0.0 | 1.0 |
| OBIMPMDI_71 | 1.0 | 0.0 | 1.0 |
| OBIMPMDI_74 | 1.0 | 0.0 | 1.0 |
| OBIMPMDI_75 | 0.0 | 1.0 | 0.0 |
| OBIMPMDI_77 | 0.0 | 1.0 | 0.0 |
| OBIMPMDI_78 | 1.0 | 0.0 | 1.0 |
| OBIMPMDI_79 | 1.0 | 0.0 | 1.0 |
| OBIMPMDI_80 | 1.0 | 0.0 | 1.0 |
| OBIMPMDI_83 | 0.0 | 1.0 | 0.0 |
| OBIMPMDI_84 | 1.0 | 0.0 | 1.0 |
| OBIMPMDI_85 | 1.0 | 0.0 | 1.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| OBIMPMDI_26 | 40425 | 74560 | False | Myoviridae | VOG3498, VOG5042, VOG3389, VOG4779, VOG1070, VOG11003, VOG9667, VOG0275, VOG7930, VOG0537, VOG7168, VOG5534, VOG6940, VOG9713, VOG6472, VOG9219, VOG6036, VOG6941, VOG7134, VOG10940, VOG5721, VOG5779, VOG5722, VOG0083 |
| OBIMPMDI_26 | 148955 | 160393 | False | Siphoviridae | VOG6211, VOG0322, VOG7476, VOG0025, VOG4551, VOG0918, VOG1198 |
Phigaro v2.4.0 (Database: Jan. 2024 release)