Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 11 | 10 | 9 | 17.38 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| FJJECKHO_53 | 59557 | 59874 | ykkD | ARO:3003064 | ykkD | 98.10 | 100.00 | AL009126.1 |
| FJJECKHO_53 | 59219 | 59557 | ykkC | ARO:3003063 | ykkC | 98.21 | 100.00 | AL009126.1 |
| FJJECKHO_50 | 41821 | 44391 | Bacillus subtilis mprF | ARO:3003324 | Bsub_mprF | 99.77 | 100.00 | AL009126.3 |
| FJJECKHO_11 | 17544 | 18977 | lmrB | ARO:3002813 | lmrB | 98.95 | 100.00 | JYFL01000006.1 |
| FJJECKHO_36 | 7180 | 7773 | tmrB | ARO:3003059 | tmrB | 99.49 | 100.00 | AL009126.3 |
| FJJECKHO_65 | 6534 | 7736 | blt | ARO:3003006 | blt | 99.25 | 100.00 | L32599.1 |
| FJJECKHO_11 | 4887 | 5651 | mphK | ARO:3004541 | mphK | 97.61 | 83.01 | CP010314.1 |
| FJJECKHO_70 | 3788 | 4642 | aadK | ARO:3002627 | aadK | 98.94 | 100.00 | AL009126.1 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| FJJECKHO_70 | 3788 | 4642 | aadK | Streptomycin | 98.83 | 100.00 | M26879 |
| FJJECKHO_11 | 4893 | 5651 | mph(K) | Spiramycin, Telithromycin | 97.63 | 82.41 | NC_000964 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| FJJECKHO_70 | 3791 | 4642 | aadK | aminoglycoside 6-adenylyltransferase AadK | BLASTX | 98.94 | 100.00 | WP_003229862.1 |
| FJJECKHO_11 | 4893 | 5639 | mphK | macrolide 2'-phosphotransferase MphK | PARTIALX | 98.39 | 81.37 | WP_003246254.1 |
| FJJECKHO_106 | 5908 | 7515 | vmlR | ABC-F type ribosomal protection protein VmlR | INTERNAL_STOP | 95.61 | 100.00 | WP_003234144.1 |
| FJJECKHO_25 | 37020 | 37538 | satA | streptothricin N-acetyltransferase SatA | BLASTX | 91.91 | 100.00 | WP_003242546.1 |
| FJJECKHO_37 | 73498 | 76095 | rphC | rifamycin-inactivating phosphotransferase RphC | BLASTX | 80.48 | 100.00 | WP_087347987.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| FJJECKHO_78 | 21515 | 22696 | capB | 0.0 | Capsule | Immune modulation | 99.66 | 100.00 | NP_391471 |
| FJJECKHO_63 | 103457 | 104242 | dhbA | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.49 | 100.00 | NP_391080 |
| FJJECKHO_78 | 23179 | 24321 | capA | 0.0 | Capsule | Immune modulation | 99.30 | 100.00 | NP_391469 |
| FJJECKHO_63 | 99621 | 100559 | dhbB | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.15 | 100.00 | NP_391077 |
| FJJECKHO_63 | 102235 | 103431 | dhbC | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.08 | 100.00 | NP_391079 |
| FJJECKHO_31 | 15364 | 16005 | hlyIII | 0.0 | Hemolysin III | Exotoxin | 98.91 | 100.00 | NP_390062 |
| FJJECKHO_78 | 22711 | 23160 | capC | 0.0 | Capsule | Immune modulation | 98.89 | 100.00 | NP_391470 |
| FJJECKHO_63 | 92465 | 99601 | dhbF | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.66 | 100.00 | NP_391076 |
| FJJECKHO_37 | 20696 | 22448 | dep/capD | 0.0 | Capsule | Immune modulation | 98.36 | 99.94 | NP_389723 |
| FJJECKHO_63 | 100587 | 102206 | dhbE | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 97.04 | 100.00 | NP_391078 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| FJJECKHO_3 | 31842 | 32978 | tufA | 0.0 | elongation factor Tu | 85.75 | 95.69 | AHM69299 |
| FJJECKHO_6 | 11360 | 10491 | pdxS | 1.99e-151 | encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS | 83.45 | 98.31 | ACE61268 |
| FJJECKHO_35 | 96879 | 96103 | CodY | 2.05e-125 | nutrient-responsive regulator | 81.08 | 100.00 | ACJ80679 |
| FJJECKHO_20 | 22551 | 21982 | ClpP | 7.68e-100 | part of proteolytic complex | 81.05 | 97.44 | BAB94595 |
| FJJECKHO_47 | 20150 | 20542 | spxA1 | 4.27e-71 | redox-responsive transcription factor | 83.97 | 100.00 | AAF21893 |
| FJJECKHO_47 | 20150 | 20542 | spx | 2.55e-69 | essential gene which is a thiol/oxidative stress sensor | 80.15 | 100.00 | BAB57159 |
| FJJECKHO_16 | 20603 | 20845 | SpoVG | 7.51e-39 | RNA-binding protein | 81.48 | 79.41 | AAT02994 |
| FJJECKHO_55 | 841 | 1038 | CspA | 1.28e-33 | cold shock protein | 90.91 | 100.00 | CAA62903 |
| FJJECKHO_31 | 27235 | 27429 | CspD | 6.95e-29 | cold shock protein | 81.54 | 98.48 | CAC99957 |
| FJJECKHO_30 | 25172 | 24978 | CspB | 4.62e-28 | cold shock protein | 83.08 | 98.48 | CAD00094 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| FJJECKHO_82 | 2647 | 2840 | Col(SD853) | 194 / 194 | 97.42 | NC015392 |
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| FJJECKHO_1 | 0.0 | 1.0 | 0.0 |
| FJJECKHO_2 | 0.0 | 1.0 | 0.0 |
| FJJECKHO_3 | 0.0 | 1.0 | 0.0 |
| FJJECKHO_4 | 0.0 | 1.0 | 0.0 |
| FJJECKHO_6 | 0.0 | 1.0 | 0.0 |
| FJJECKHO_7 | 0.0 | 1.0 | 0.0 |
| FJJECKHO_9 | 0.0 | 1.0 | 0.0 |
| FJJECKHO_10 | 0.0 | 1.0 | 0.0 |
| FJJECKHO_11 | 0.0 | 1.0 | 0.0 |
| FJJECKHO_12 | 0.0 | 1.0 | 0.0 |
| FJJECKHO_13 | 0.0 | 1.0 | 0.0 |
| FJJECKHO_14 | 0.0 | 1.0 | 0.0 |
| FJJECKHO_15 | 0.0 | 1.0 | 0.0 |
| FJJECKHO_16 | 0.0 | 1.0 | 0.0 |
| FJJECKHO_17 | 0.0 | 1.0 | 0.0 |
| FJJECKHO_18 | 0.0 | 1.0 | 0.0 |
| FJJECKHO_19 | 0.0 | 1.0 | 0.0 |
| FJJECKHO_20 | 0.0 | 1.0 | 0.0 |
| FJJECKHO_21 | 0.0 | 1.0 | 0.0 |
| FJJECKHO_22 | 0.0 | 1.0 | 0.0 |
| FJJECKHO_23 | 0.0 | 1.0 | 0.0 |
| FJJECKHO_24 | 0.0 | 1.0 | 0.0 |
| FJJECKHO_25 | 0.0 | 1.0 | 0.0 |
| FJJECKHO_26 | 0.0 | 1.0 | 0.0 |
| FJJECKHO_27 | 0.0 | 1.0 | 0.0 |
| FJJECKHO_28 | 0.0 | 1.0 | 0.0 |
| FJJECKHO_29 | 0.0 | 1.0 | 0.0 |
| FJJECKHO_30 | 0.0 | 1.0 | 0.0 |
| FJJECKHO_31 | 0.0 | 1.0 | 0.0 |
| FJJECKHO_32 | 0.0 | 1.0 | 0.0 |
| FJJECKHO_34 | 0.0 | 1.0 | 0.0 |
| FJJECKHO_35 | 0.0 | 1.0 | 0.0 |
| FJJECKHO_36 | 0.0 | 1.0 | 0.0 |
| FJJECKHO_37 | 0.0 | 1.0 | 0.0 |
| FJJECKHO_39 | 0.0 | 1.0 | 0.0 |
| FJJECKHO_40 | 0.0 | 1.0 | 0.0 |
| FJJECKHO_41 | 0.0 | 1.0 | 0.0 |
| FJJECKHO_42 | 0.0 | 1.0 | 0.0 |
| FJJECKHO_44 | 0.0 | 1.0 | 0.0 |
| FJJECKHO_45 | 0.0 | 1.0 | 0.0 |
| FJJECKHO_46 | 0.0 | 1.0 | 0.0 |
| FJJECKHO_47 | 0.0 | 1.0 | 0.0 |
| FJJECKHO_48 | 0.0 | 1.0 | 0.0 |
| FJJECKHO_49 | 0.0 | 1.0 | 0.0 |
| FJJECKHO_50 | 0.0 | 1.0 | 0.0 |
| FJJECKHO_51 | 0.0 | 1.0 | 0.0 |
| FJJECKHO_52 | 0.0 | 1.0 | 0.0 |
| FJJECKHO_53 | 0.0 | 1.0 | 0.0 |
| FJJECKHO_54 | 0.0 | 1.0 | 0.0 |
| FJJECKHO_55 | 1.0 | 0.0 | 1.0 |
| FJJECKHO_56 | 0.0 | 1.0 | 0.0 |
| FJJECKHO_57 | 0.0 | 1.0 | 0.0 |
| FJJECKHO_58 | 0.0 | 1.0 | 0.0 |
| FJJECKHO_59 | 0.0 | 1.0 | 0.0 |
| FJJECKHO_60 | 0.0 | 1.0 | 0.0 |
| FJJECKHO_61 | 0.0 | 1.0 | 0.0 |
| FJJECKHO_62 | 0.0 | 1.0 | 0.0 |
| FJJECKHO_63 | 0.0 | 1.0 | 0.0 |
| FJJECKHO_64 | 0.0 | 1.0 | 0.0 |
| FJJECKHO_65 | 0.0 | 1.0 | 0.0 |
| FJJECKHO_66 | 0.0 | 1.0 | 0.0 |
| FJJECKHO_67 | 0.0 | 1.0 | 0.0 |
| FJJECKHO_68 | 0.0 | 1.0 | 0.0 |
| FJJECKHO_69 | 0.0 | 1.0 | 0.0 |
| FJJECKHO_70 | 0.0 | 1.0 | 0.0 |
| FJJECKHO_71 | 0.0 | 1.0 | 0.0 |
| FJJECKHO_72 | 0.0 | 1.0 | 0.0 |
| FJJECKHO_73 | 0.0 | 1.0 | 0.0 |
| FJJECKHO_74 | 0.0 | 1.0 | 0.0 |
| FJJECKHO_75 | 0.0 | 1.0 | 0.0 |
| FJJECKHO_76 | 0.0 | 1.0 | 0.0 |
| FJJECKHO_77 | 0.0 | 1.0 | 0.0 |
| FJJECKHO_78 | 0.0 | 1.0 | 0.0 |
| FJJECKHO_79 | 0.0 | 1.0 | 0.0 |
| FJJECKHO_80 | 0.0 | 1.0 | 0.0 |
| FJJECKHO_81 | 0.0 | 1.0 | 0.0 |
| FJJECKHO_82 | 1.0 | 0.0 | 1.0 |
| FJJECKHO_83 | 0.0 | 1.0 | 0.0 |
| FJJECKHO_84 | 0.0 | 1.0 | 0.0 |
| FJJECKHO_85 | 0.0 | 1.0 | 0.0 |
| FJJECKHO_86 | 0.0 | 1.0 | 0.0 |
| FJJECKHO_87 | 0.0 | 1.0 | 0.0 |
| FJJECKHO_88 | 0.0 | 1.0 | 0.0 |
| FJJECKHO_89 | 1.0 | 0.0 | 1.0 |
| FJJECKHO_90 | 0.0 | 1.0 | 0.0 |
| FJJECKHO_91 | 0.0 | 1.0 | 0.0 |
| FJJECKHO_92 | 0.0 | 1.0 | 0.0 |
| FJJECKHO_93 | 0.0 | 1.0 | 0.0 |
| FJJECKHO_94 | 0.0 | 1.0 | 0.0 |
| FJJECKHO_95 | 0.0 | 1.0 | 0.0 |
| FJJECKHO_97 | 0.0 | 1.0 | 0.0 |
| FJJECKHO_98 | 0.0 | 1.0 | 0.0 |
| FJJECKHO_99 | 0.0 | 1.0 | 0.0 |
| FJJECKHO_100 | 0.0 | 1.0 | 0.0 |
| FJJECKHO_101 | 0.0 | 1.0 | 0.0 |
| FJJECKHO_102 | 0.0 | 1.0 | 0.0 |
| FJJECKHO_103 | 1.0 | 0.0 | 1.0 |
| FJJECKHO_104 | 0.0 | 1.0 | 0.0 |
| FJJECKHO_105 | 1.0 | 0.0 | 1.0 |
| FJJECKHO_106 | 0.0 | 1.0 | 0.0 |
| FJJECKHO_107 | 0.0 | 1.0 | 0.0 |
| FJJECKHO_108 | 1.0 | 0.0 | 1.0 |
| FJJECKHO_109 | 0.0 | 1.0 | 0.0 |
| FJJECKHO_110 | 0.0 | 1.0 | 0.0 |
| FJJECKHO_111 | 0.0 | 1.0 | 0.0 |
| FJJECKHO_112 | 0.0 | 1.0 | 0.0 |
| FJJECKHO_113 | 0.0 | 1.0 | 0.0 |
| FJJECKHO_114 | 0.0 | 1.0 | 0.0 |
| FJJECKHO_115 | 0.0 | 1.0 | 0.0 |
| FJJECKHO_116 | 0.0 | 1.0 | 0.0 |
| FJJECKHO_117 | 0.0 | 1.0 | 0.0 |
| FJJECKHO_120 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| FJJECKHO_20 | 109134 | 120822 | True | Siphoviridae | VOG3490, VOG0275, VOG0275, VOG1563, VOG9667, VOG9667, VOG7581, VOG4566, VOG0189, VOG6587, VOG1336 |
| FJJECKHO_21 | 466 | 25697 | False | Siphoviridae | VOG4619, VOG0801, VOG6163, VOG5910, VOG0541, VOG4586, VOG0704, VOG0539, VOG4589, VOG0204, VOG5361, VOG4553, VOG4573, VOG4556, VOG0202, VOG1886, VOG4581, VOG4841, VOG0713, VOG7884, VOG0275, VOG0198, VOG0044, VOG0397, VOG3444, VOG4567, VOG5836, VOG1336, VOG0536 |
| FJJECKHO_53 | 3599 | 29755 | False | Myoviridae | VOG3490, VOG0189, VOG6875, VOG4861, VOG5447, VOG0796, VOG10227, VOG4773, VOG4685, VOG0749, VOG3890, VOG3891, VOG3892, VOG6163, VOG4865, VOG4885, VOG3894, VOG4550, VOG4691, VOG4862, VOG7718, VOG4705 |
Phigaro v2.4.0 (Database: Jan. 2024 release)