Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 11 | 10 | 9 | 17.38 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| BILODBNM_49 | 62390 | 62707 | ykkD | ARO:3003064 | ykkD | 98.10 | 100.00 | AL009126.1 |
| BILODBNM_49 | 62052 | 62390 | ykkC | ARO:3003063 | ykkC | 98.21 | 100.00 | AL009126.1 |
| BILODBNM_23 | 49049 | 50695 | vmlR | ARO:3004476 | vmlR | 98.18 | 100.18 | NC_000964.3 |
| BILODBNM_25 | 20862 | 22301 | lmrB | ARO:3002813 | lmrB | 99.58 | 100.42 | JYFL01000006.1 |
| BILODBNM_20 | 15261 | 16112 | aadK | ARO:3002627 | aadK | 98.24 | 99.65 | AL009126.1 |
| BILODBNM_7 | 9348 | 11918 | Bacillus subtilis mprF | ARO:3003324 | Bsub_mprF | 99.77 | 100.00 | AL009126.3 |
| BILODBNM_65 | 6845 | 8047 | blt | ARO:3003006 | blt | 99.25 | 100.00 | L32599.1 |
| BILODBNM_73 | 954 | 1547 | tmrB | ARO:3003059 | tmrB | 97.97 | 100.00 | AL009126.3 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| BILODBNM_20 | 15261 | 16112 | aadK | Streptomycin | 98.48 | 99.65 | M26879 |
| BILODBNM_25 | 34194 | 35113 | mph(K) | Spiramycin, Telithromycin | 97.72 | 99.89 | NC_000964 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| BILODBNM_20 | 15264 | 16112 | aadK | aminoglycoside 6-adenylyltransferase AadK | BLASTX | 98.24 | 100.00 | WP_003229862.1 |
| BILODBNM_23 | 49052 | 50695 | vmlR | ABC-F type ribosomal protection protein VmlR | BLASTX | 98.18 | 100.00 | WP_003234144.1 |
| BILODBNM_31 | 37288 | 37806 | satA | streptothricin N-acetyltransferase SatA | BLASTX | 93.06 | 100.00 | WP_003242546.1 |
| BILODBNM_29 | 135282 | 137879 | rphC | rifamycin-inactivating phosphotransferase RphC | BLASTX | 80.48 | 100.00 | WP_087347987.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| BILODBNM_36 | 69125 | 70306 | capB | 0.0 | Capsule | Immune modulation | 99.66 | 100.00 | NP_391471 |
| BILODBNM_40 | 103016 | 103801 | dhbA | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.36 | 100.00 | NP_391080 |
| BILODBNM_36 | 67500 | 68642 | capA | 0.0 | Capsule | Immune modulation | 99.30 | 100.00 | NP_391469 |
| BILODBNM_29 | 82342 | 84105 | dep/capD | 0.0 | Capsule | Immune modulation | 99.09 | 100.00 | NP_389723 |
| BILODBNM_40 | 99191 | 100129 | dhbB | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.94 | 100.00 | NP_391077 |
| BILODBNM_36 | 68661 | 69110 | capC | 0.0 | Capsule | Immune modulation | 98.89 | 100.00 | NP_391470 |
| BILODBNM_40 | 101794 | 102990 | dhbC | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.58 | 100.00 | NP_391079 |
| BILODBNM_40 | 92035 | 99171 | dhbF | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.57 | 100.00 | NP_391076 |
| BILODBNM_24 | 29033 | 29674 | hlyIII | 0.0 | Hemolysin III | Exotoxin | 97.66 | 100.00 | NP_390062 |
| BILODBNM_40 | 100157 | 101776 | dhbE | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 97.28 | 100.00 | NP_391078 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| BILODBNM_43 | 31842 | 32978 | tufA | 0.0 | elongation factor Tu | 85.75 | 95.69 | AHM69299 |
| BILODBNM_68 | 11730 | 10861 | pdxS | 1.18e-151 | encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS | 83.79 | 98.31 | ACE61268 |
| BILODBNM_18 | 112763 | 111987 | CodY | 1.85e-125 | nutrient-responsive regulator | 81.08 | 100.00 | ACJ80679 |
| BILODBNM_27 | 34240 | 33671 | ClpP | 9.36e-100 | part of proteolytic complex | 81.05 | 97.44 | BAB94595 |
| BILODBNM_10 | 28659 | 28267 | spxA1 | 3.31e-71 | redox-responsive transcription factor | 83.97 | 100.00 | AAF21893 |
| BILODBNM_10 | 28659 | 28267 | spx | 1.97e-69 | essential gene which is a thiol/oxidative stress sensor | 80.15 | 100.00 | BAB57159 |
| BILODBNM_66 | 11141 | 11383 | SpoVG | 6.23e-39 | RNA-binding protein | 81.48 | 79.41 | AAT02994 |
| BILODBNM_23 | 5261 | 5458 | CspA | 3.44e-32 | cold shock protein | 90.91 | 100.00 | CAA62903 |
| BILODBNM_24 | 40904 | 41098 | CspD | 7.79e-29 | cold shock protein | 81.54 | 98.48 | CAC99957 |
| BILODBNM_4 | 31754 | 31560 | CspB | 1.86e-27 | cold shock protein | 83.08 | 98.48 | CAD00094 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| BILODBNM_54 | 2378 | 2538 | Col(SD853) | 161 / 194 | 83.23 | NC015392 |
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| BILODBNM_1 | 0.0 | 1.0 | 0.0 |
| BILODBNM_2 | 0.0 | 1.0 | 0.0 |
| BILODBNM_3 | 0.0 | 1.0 | 0.0 |
| BILODBNM_4 | 0.0 | 1.0 | 0.0 |
| BILODBNM_6 | 0.0 | 1.0 | 0.0 |
| BILODBNM_7 | 0.0 | 1.0 | 0.0 |
| BILODBNM_8 | 0.0 | 1.0 | 0.0 |
| BILODBNM_9 | 1.0 | 0.0 | 1.0 |
| BILODBNM_10 | 0.0 | 1.0 | 0.0 |
| BILODBNM_11 | 0.0 | 1.0 | 0.0 |
| BILODBNM_12 | 1.0 | 0.0 | 1.0 |
| BILODBNM_13 | 0.0 | 1.0 | 0.0 |
| BILODBNM_14 | 0.0 | 1.0 | 0.0 |
| BILODBNM_15 | 0.0 | 1.0 | 0.0 |
| BILODBNM_16 | 0.0 | 1.0 | 0.0 |
| BILODBNM_17 | 0.0 | 1.0 | 0.0 |
| BILODBNM_18 | 0.0 | 1.0 | 0.0 |
| BILODBNM_19 | 0.0 | 1.0 | 0.0 |
| BILODBNM_20 | 0.0 | 1.0 | 0.0 |
| BILODBNM_21 | 0.0 | 1.0 | 0.0 |
| BILODBNM_22 | 0.0 | 1.0 | 0.0 |
| BILODBNM_23 | 0.0 | 1.0 | 0.0 |
| BILODBNM_24 | 0.0 | 1.0 | 0.0 |
| BILODBNM_25 | 0.0 | 1.0 | 0.0 |
| BILODBNM_26 | 0.0 | 1.0 | 0.0 |
| BILODBNM_27 | 0.0 | 1.0 | 0.0 |
| BILODBNM_28 | 0.0 | 1.0 | 0.0 |
| BILODBNM_29 | 0.0 | 1.0 | 0.0 |
| BILODBNM_30 | 0.0 | 1.0 | 0.0 |
| BILODBNM_31 | 0.0 | 1.0 | 0.0 |
| BILODBNM_32 | 0.0 | 1.0 | 0.0 |
| BILODBNM_33 | 1.0 | 0.0 | 1.0 |
| BILODBNM_34 | 0.0 | 1.0 | 0.0 |
| BILODBNM_35 | 0.0 | 1.0 | 0.0 |
| BILODBNM_36 | 0.0 | 1.0 | 0.0 |
| BILODBNM_37 | 1.0 | 0.0 | 1.0 |
| BILODBNM_38 | 0.0 | 1.0 | 0.0 |
| BILODBNM_39 | 0.0 | 1.0 | 0.0 |
| BILODBNM_40 | 0.0 | 1.0 | 0.0 |
| BILODBNM_41 | 0.0 | 1.0 | 0.0 |
| BILODBNM_42 | 0.0 | 1.0 | 0.0 |
| BILODBNM_43 | 0.0 | 1.0 | 0.0 |
| BILODBNM_44 | 0.0 | 1.0 | 0.0 |
| BILODBNM_45 | 0.0 | 1.0 | 0.0 |
| BILODBNM_46 | 0.0 | 1.0 | 0.0 |
| BILODBNM_48 | 0.0 | 1.0 | 0.0 |
| BILODBNM_49 | 0.0 | 1.0 | 0.0 |
| BILODBNM_50 | 0.0 | 1.0 | 0.0 |
| BILODBNM_51 | 0.0 | 1.0 | 0.0 |
| BILODBNM_52 | 0.0 | 1.0 | 0.0 |
| BILODBNM_53 | 0.0 | 1.0 | 0.0 |
| BILODBNM_54 | 1.0 | 0.0 | 1.0 |
| BILODBNM_55 | 0.0 | 1.0 | 0.0 |
| BILODBNM_56 | 1.0 | 0.0 | 1.0 |
| BILODBNM_57 | 0.0 | 1.0 | 0.0 |
| BILODBNM_58 | 0.0 | 1.0 | 0.0 |
| BILODBNM_59 | 0.0 | 1.0 | 0.0 |
| BILODBNM_60 | 0.0 | 1.0 | 0.0 |
| BILODBNM_61 | 0.0 | 1.0 | 0.0 |
| BILODBNM_62 | 0.0 | 1.0 | 0.0 |
| BILODBNM_63 | 0.0 | 1.0 | 0.0 |
| BILODBNM_64 | 0.0 | 1.0 | 0.0 |
| BILODBNM_65 | 0.0 | 1.0 | 0.0 |
| BILODBNM_66 | 0.0 | 1.0 | 0.0 |
| BILODBNM_67 | 1.0 | 0.0 | 1.0 |
| BILODBNM_68 | 0.0 | 1.0 | 0.0 |
| BILODBNM_69 | 0.0 | 1.0 | 0.0 |
| BILODBNM_70 | 0.0 | 1.0 | 0.0 |
| BILODBNM_71 | 0.0 | 1.0 | 0.0 |
| BILODBNM_72 | 0.0 | 1.0 | 0.0 |
| BILODBNM_73 | 0.0 | 1.0 | 0.0 |
| BILODBNM_74 | 0.0 | 1.0 | 0.0 |
| BILODBNM_75 | 0.0 | 1.0 | 0.0 |
| BILODBNM_76 | 0.0 | 1.0 | 0.0 |
| BILODBNM_77 | 1.0 | 0.0 | 1.0 |
| BILODBNM_78 | 0.0 | 1.0 | 0.0 |
| BILODBNM_79 | 1.0 | 0.0 | 1.0 |
| BILODBNM_80 | 0.0 | 1.0 | 0.0 |
| BILODBNM_81 | 1.0 | 0.0 | 1.0 |
| BILODBNM_82 | 1.0 | 0.0 | 1.0 |
| BILODBNM_83 | 1.0 | 0.0 | 1.0 |
| BILODBNM_84 | 1.0 | 0.0 | 1.0 |
| BILODBNM_85 | 0.0 | 1.0 | 0.0 |
| BILODBNM_87 | 0.0 | 1.0 | 0.0 |
| BILODBNM_88 | 0.0 | 1.0 | 0.0 |
| BILODBNM_89 | 0.0 | 1.0 | 0.0 |
| BILODBNM_90 | 0.0 | 1.0 | 0.0 |
| BILODBNM_91 | 1.0 | 0.0 | 1.0 |
| BILODBNM_92 | 0.0 | 1.0 | 0.0 |
| BILODBNM_93 | 0.0 | 1.0 | 0.0 |
| BILODBNM_94 | 1.0 | 0.0 | 1.0 |
| BILODBNM_95 | 0.0 | 1.0 | 0.0 |
| BILODBNM_96 | 1.0 | 0.0 | 1.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| BILODBNM_2 | 273987 | 300487 | False | Myoviridae | VOG0720, VOG4555, VOG4553, VOG4564, VOG4623, VOG1353, VOG1881, VOG1883, VOG1352, VOG4699, VOG6163, VOG1956, VOG1433, VOG1348, VOG0573, VOG4550, VOG4691, VOG4845, VOG7718, VOG3390, VOG4705, VOG5008, VOG0753, VOG0136, VOG4574, VOG10547, VOG1301, VOG6116, VOG0221 |
| BILODBNM_27 | 122062 | 129796 | True | Siphoviridae | VOG3490, VOG0275, VOG0275, VOG1563, VOG9667, VOG9667, VOG7581 |
| BILODBNM_27 | 136616 | 153874 | False | Siphoviridae | VOG0198, VOG0275, VOG4841, VOG0713, VOG4581, VOG1886, VOG3607, VOG4556, VOG4568, VOG4553, VOG5361, VOG0204, VOG9583, VOG0704, VOG4586, VOG0541, VOG5910 |
| BILODBNM_49 | 5903 | 32588 | False | Myoviridae | VOG9667, VOG3490, VOG0189, VOG4861, VOG5447, VOG0796, VOG10227, VOG4773, VOG4685, VOG0749, VOG3890, VOG3891, VOG3892, VOG6163, VOG4865, VOG4885, VOG3894, VOG4550, VOG4691, VOG4862, VOG7718, VOG4705 |
Phigaro v2.4.0 (Database: Jan. 2024 release)