Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 12 | 9 | 9 | 17.49 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| BHJHNLFJ_12 | 109517 | 110950 | lmrB | ARO:3002813 | lmrB | 99.16 | 100.00 | JYFL01000006.1 |
| BHJHNLFJ_76 | 69323 | 70492 | bmr | ARO:3003007 | bmr | 99.49 | 100.00 | M33768.1 |
| BHJHNLFJ_27 | 62401 | 62718 | ykkD | ARO:3003064 | ykkD | 98.10 | 100.00 | AL009126.1 |
| BHJHNLFJ_27 | 62063 | 62401 | ykkC | ARO:3003063 | ykkC | 98.21 | 100.00 | AL009126.1 |
| BHJHNLFJ_20 | 51841 | 54411 | Bacillus subtilis mprF | ARO:3003324 | Bsub_mprF | 99.77 | 100.00 | AL009126.3 |
| BHJHNLFJ_12 | 46431 | 47024 | tmrB | ARO:3003059 | tmrB | 100.00 | 100.00 | AL009126.3 |
| BHJHNLFJ_58 | 13092 | 14735 | vmlR | ARO:3004476 | vmlR | 98.32 | 100.00 | NC_000964.3 |
| BHJHNLFJ_115 | 9328 | 10179 | aadK | ARO:3002627 | aadK | 98.24 | 99.65 | AL009126.1 |
| BHJHNLFJ_45 | 6406 | 7326 | mphK | ARO:3004541 | mphK | 98.68 | 100.00 | CP010314.1 |
| BHJHNLFJ_16 | 762 | 1964 | blt | ARO:3003006 | blt | 99.25 | 100.00 | L32599.1 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| BHJHNLFJ_115 | 9328 | 10179 | aadK | Streptomycin | 98.48 | 99.65 | M26879 |
| BHJHNLFJ_45 | 6406 | 7326 | mph(K) | Spiramycin, Telithromycin | 98.05 | 100.00 | NC_000964 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| BHJHNLFJ_45 | 6418 | 7326 | mphK | macrolide 2'-phosphotransferase MphK | BLASTX | 98.68 | 99.02 | WP_003246254.1 |
| BHJHNLFJ_115 | 9328 | 10176 | aadK | aminoglycoside 6-adenylyltransferase AadK | BLASTX | 98.24 | 100.00 | WP_003229862.1 |
| BHJHNLFJ_58 | 13095 | 14735 | vmlR | ABC-F type ribosomal protection protein VmlR | BLASTX | 96.71 | 99.82 | WP_003234144.1 |
| BHJHNLFJ_17 | 1433 | 1825 | satA | streptothricin N-acetyltransferase SatA | PARTIALX | 91.60 | 75.72 | WP_003242546.1 |
| BHJHNLFJ_64 | 106296 | 108893 | rphC | rifamycin-inactivating phosphotransferase RphC | BLASTX | 80.48 | 100.00 | WP_087347987.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| BHJHNLFJ_14 | 113393 | 114574 | capB | 0.0 | Capsule | Immune modulation | 99.66 | 100.00 | NP_391471 |
| BHJHNLFJ_9 | 108007 | 108792 | dhbA | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.36 | 100.00 | NP_391080 |
| BHJHNLFJ_14 | 115057 | 116199 | capA | 0.0 | Capsule | Immune modulation | 99.30 | 100.00 | NP_391469 |
| BHJHNLFJ_9 | 104182 | 105120 | dhbB | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.94 | 100.00 | NP_391077 |
| BHJHNLFJ_73 | 277 | 918 | hlyIII | 0.0 | Hemolysin III | Exotoxin | 98.91 | 100.00 | NP_390062 |
| BHJHNLFJ_14 | 114589 | 115038 | capC | 0.0 | Capsule | Immune modulation | 98.89 | 100.00 | NP_391470 |
| BHJHNLFJ_9 | 106785 | 107981 | dhbC | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.75 | 100.00 | NP_391079 |
| BHJHNLFJ_9 | 97026 | 104162 | dhbF | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.57 | 100.00 | NP_391076 |
| BHJHNLFJ_9 | 105148 | 106767 | dhbE | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 97.28 | 100.00 | NP_391078 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| BHJHNLFJ_48 | 28040 | 26904 | tufA | 0.0 | elongation factor Tu | 85.75 | 95.69 | AHM69299 |
| BHJHNLFJ_39 | 4325 | 5194 | pdxS | 1.11e-151 | encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS | 83.79 | 98.31 | ACE61268 |
| BHJHNLFJ_25 | 96878 | 96102 | CodY | 1.38e-125 | nutrient-responsive regulator | 81.08 | 100.00 | ACJ80679 |
| BHJHNLFJ_13 | 45208 | 44639 | ClpP | 8.53e-100 | part of proteolytic complex | 81.05 | 97.44 | BAB94595 |
| BHJHNLFJ_44 | 23405 | 23797 | spxA1 | 4.12e-71 | redox-responsive transcription factor | 83.97 | 100.00 | AAF21893 |
| BHJHNLFJ_44 | 23405 | 23797 | spx | 2.46e-69 | essential gene which is a thiol/oxidative stress sensor | 80.15 | 100.00 | BAB57159 |
| BHJHNLFJ_80 | 27111 | 26869 | SpoVG | 5.21e-39 | RNA-binding protein | 81.48 | 79.41 | AAT02994 |
| BHJHNLFJ_35 | 74968 | 75165 | CspA | 3.33e-32 | cold shock protein | 90.91 | 100.00 | CAA62903 |
| BHJHNLFJ_33 | 77820 | 77626 | CspD | 5.49e-29 | cold shock protein | 81.54 | 98.48 | CAC99957 |
| BHJHNLFJ_26 | 25124 | 24930 | CspB | 6.84e-28 | cold shock protein | 83.08 | 98.48 | CAD00094 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| BHJHNLFJ_1 | 0.0 | 1.0 | 0.0 |
| BHJHNLFJ_2 | 0.0 | 1.0 | 0.0 |
| BHJHNLFJ_3 | 0.0 | 1.0 | 0.0 |
| BHJHNLFJ_4 | 0.0 | 1.0 | 0.0 |
| BHJHNLFJ_5 | 0.0 | 1.0 | 0.0 |
| BHJHNLFJ_6 | 0.0 | 1.0 | 0.0 |
| BHJHNLFJ_7 | 0.0 | 1.0 | 0.0 |
| BHJHNLFJ_8 | 0.0 | 1.0 | 0.0 |
| BHJHNLFJ_9 | 0.0 | 1.0 | 0.0 |
| BHJHNLFJ_10 | 0.0 | 1.0 | 0.0 |
| BHJHNLFJ_11 | 0.0 | 1.0 | 0.0 |
| BHJHNLFJ_12 | 0.0 | 1.0 | 0.0 |
| BHJHNLFJ_13 | 0.0 | 1.0 | 0.0 |
| BHJHNLFJ_14 | 0.0 | 1.0 | 0.0 |
| BHJHNLFJ_15 | 0.0 | 1.0 | 0.0 |
| BHJHNLFJ_16 | 0.0 | 1.0 | 0.0 |
| BHJHNLFJ_17 | 0.0 | 1.0 | 0.0 |
| BHJHNLFJ_18 | 0.0 | 1.0 | 0.0 |
| BHJHNLFJ_19 | 0.0 | 1.0 | 0.0 |
| BHJHNLFJ_20 | 0.0 | 1.0 | 0.0 |
| BHJHNLFJ_21 | 0.0 | 1.0 | 0.0 |
| BHJHNLFJ_22 | 0.0 | 1.0 | 0.0 |
| BHJHNLFJ_23 | 0.0 | 1.0 | 0.0 |
| BHJHNLFJ_24 | 0.0 | 1.0 | 0.0 |
| BHJHNLFJ_25 | 0.0 | 1.0 | 0.0 |
| BHJHNLFJ_26 | 0.0 | 1.0 | 0.0 |
| BHJHNLFJ_27 | 0.0 | 1.0 | 0.0 |
| BHJHNLFJ_28 | 0.0 | 1.0 | 0.0 |
| BHJHNLFJ_29 | 0.0 | 1.0 | 0.0 |
| BHJHNLFJ_30 | 0.0 | 1.0 | 0.0 |
| BHJHNLFJ_31 | 0.0 | 1.0 | 0.0 |
| BHJHNLFJ_32 | 0.0 | 1.0 | 0.0 |
| BHJHNLFJ_33 | 0.0 | 1.0 | 0.0 |
| BHJHNLFJ_34 | 0.0 | 1.0 | 0.0 |
| BHJHNLFJ_35 | 0.0 | 1.0 | 0.0 |
| BHJHNLFJ_36 | 0.0 | 1.0 | 0.0 |
| BHJHNLFJ_37 | 0.0 | 1.0 | 0.0 |
| BHJHNLFJ_38 | 1.0 | 0.0 | 1.0 |
| BHJHNLFJ_39 | 0.0 | 1.0 | 0.0 |
| BHJHNLFJ_40 | 0.0 | 1.0 | 0.0 |
| BHJHNLFJ_41 | 0.0 | 1.0 | 0.0 |
| BHJHNLFJ_42 | 0.0 | 1.0 | 0.0 |
| BHJHNLFJ_43 | 0.0 | 1.0 | 0.0 |
| BHJHNLFJ_44 | 0.0 | 1.0 | 0.0 |
| BHJHNLFJ_45 | 0.0 | 1.0 | 0.0 |
| BHJHNLFJ_46 | 0.0 | 1.0 | 0.0 |
| BHJHNLFJ_47 | 0.0 | 1.0 | 0.0 |
| BHJHNLFJ_48 | 0.0 | 1.0 | 0.0 |
| BHJHNLFJ_49 | 0.0 | 1.0 | 0.0 |
| BHJHNLFJ_50 | 0.0 | 1.0 | 0.0 |
| BHJHNLFJ_51 | 0.0 | 1.0 | 0.0 |
| BHJHNLFJ_52 | 0.0 | 1.0 | 0.0 |
| BHJHNLFJ_53 | 0.0 | 1.0 | 0.0 |
| BHJHNLFJ_54 | 0.0 | 1.0 | 0.0 |
| BHJHNLFJ_55 | 1.0 | 0.0 | 1.0 |
| BHJHNLFJ_56 | 0.0 | 1.0 | 0.0 |
| BHJHNLFJ_57 | 0.0 | 1.0 | 0.0 |
| BHJHNLFJ_58 | 0.0 | 1.0 | 0.0 |
| BHJHNLFJ_59 | 0.0 | 1.0 | 0.0 |
| BHJHNLFJ_60 | 0.0 | 1.0 | 0.0 |
| BHJHNLFJ_61 | 0.0 | 1.0 | 0.0 |
| BHJHNLFJ_62 | 0.0 | 1.0 | 0.0 |
| BHJHNLFJ_63 | 0.0 | 1.0 | 0.0 |
| BHJHNLFJ_64 | 0.0 | 1.0 | 0.0 |
| BHJHNLFJ_65 | 0.0 | 1.0 | 0.0 |
| BHJHNLFJ_67 | 0.0 | 1.0 | 0.0 |
| BHJHNLFJ_68 | 0.0 | 1.0 | 0.0 |
| BHJHNLFJ_69 | 0.0 | 1.0 | 0.0 |
| BHJHNLFJ_70 | 0.0 | 1.0 | 0.0 |
| BHJHNLFJ_71 | 0.0 | 1.0 | 0.0 |
| BHJHNLFJ_72 | 0.0 | 1.0 | 0.0 |
| BHJHNLFJ_73 | 0.0 | 1.0 | 0.0 |
| BHJHNLFJ_74 | 0.0 | 1.0 | 0.0 |
| BHJHNLFJ_75 | 0.0 | 1.0 | 0.0 |
| BHJHNLFJ_76 | 0.0 | 1.0 | 0.0 |
| BHJHNLFJ_77 | 0.0 | 1.0 | 0.0 |
| BHJHNLFJ_78 | 0.0 | 1.0 | 0.0 |
| BHJHNLFJ_79 | 0.0 | 1.0 | 0.0 |
| BHJHNLFJ_80 | 0.0 | 1.0 | 0.0 |
| BHJHNLFJ_81 | 0.0 | 1.0 | 0.0 |
| BHJHNLFJ_83 | 0.0 | 1.0 | 0.0 |
| BHJHNLFJ_84 | 0.0 | 1.0 | 0.0 |
| BHJHNLFJ_85 | 0.0 | 1.0 | 0.0 |
| BHJHNLFJ_86 | 0.0 | 1.0 | 0.0 |
| BHJHNLFJ_87 | 0.0 | 1.0 | 0.0 |
| BHJHNLFJ_88 | 0.0 | 1.0 | 0.0 |
| BHJHNLFJ_89 | 0.0 | 1.0 | 0.0 |
| BHJHNLFJ_90 | 1.0 | 0.0 | 1.0 |
| BHJHNLFJ_92 | 0.0 | 1.0 | 0.0 |
| BHJHNLFJ_93 | 0.0 | 1.0 | 0.0 |
| BHJHNLFJ_94 | 0.0 | 1.0 | 0.0 |
| BHJHNLFJ_95 | 0.0 | 1.0 | 0.0 |
| BHJHNLFJ_97 | 0.0 | 1.0 | 0.0 |
| BHJHNLFJ_98 | 0.0 | 1.0 | 0.0 |
| BHJHNLFJ_99 | 0.0 | 1.0 | 0.0 |
| BHJHNLFJ_100 | 0.0 | 1.0 | 0.0 |
| BHJHNLFJ_101 | 0.0 | 1.0 | 0.0 |
| BHJHNLFJ_102 | 0.0 | 1.0 | 0.0 |
| BHJHNLFJ_103 | 0.0 | 1.0 | 0.0 |
| BHJHNLFJ_104 | 0.0 | 1.0 | 0.0 |
| BHJHNLFJ_105 | 0.0 | 1.0 | 0.0 |
| BHJHNLFJ_106 | 0.0 | 1.0 | 0.0 |
| BHJHNLFJ_107 | 0.0 | 1.0 | 0.0 |
| BHJHNLFJ_109 | 0.0 | 1.0 | 0.0 |
| BHJHNLFJ_110 | 0.0 | 1.0 | 0.0 |
| BHJHNLFJ_111 | 1.0 | 0.0 | 1.0 |
| BHJHNLFJ_112 | 0.0 | 1.0 | 0.0 |
| BHJHNLFJ_113 | 0.0 | 1.0 | 0.0 |
| BHJHNLFJ_114 | 0.0 | 1.0 | 0.0 |
| BHJHNLFJ_115 | 0.0 | 1.0 | 0.0 |
| BHJHNLFJ_116 | 0.0 | 1.0 | 0.0 |
| BHJHNLFJ_117 | 1.0 | 0.0 | 1.0 |
| BHJHNLFJ_118 | 0.0 | 1.0 | 0.0 |
| BHJHNLFJ_120 | 0.0 | 1.0 | 0.0 |
| BHJHNLFJ_121 | 0.0 | 1.0 | 0.0 |
| BHJHNLFJ_122 | 0.0 | 1.0 | 0.0 |
| BHJHNLFJ_123 | 0.0 | 1.0 | 0.0 |
| BHJHNLFJ_124 | 0.0 | 1.0 | 0.0 |
| BHJHNLFJ_127 | 0.0 | 1.0 | 0.0 |
| BHJHNLFJ_128 | 0.0 | 1.0 | 0.0 |
| BHJHNLFJ_129 | 0.0 | 1.0 | 0.0 |
| BHJHNLFJ_130 | 0.0 | 1.0 | 0.0 |
| BHJHNLFJ_134 | 0.0 | 1.0 | 0.0 |
| BHJHNLFJ_135 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| BHJHNLFJ_5 | 1 | 18921 | False | Siphoviridae | VOG4626, VOG4619, VOG0801, VOG6163, VOG5910, VOG0541, VOG4586, VOG0704, VOG0539, VOG4589, VOG0204, VOG5361, VOG4553, VOG4568, VOG4556, VOG2935, VOG4544, VOG4581, VOG4556, VOG4841, VOG0531 |
| BHJHNLFJ_13 | 131781 | 138196 | True | Siphoviridae | VOG3490, VOG0275, VOG0275, VOG1563 |
| BHJHNLFJ_27 | 5156 | 32598 | False | Myoviridae | VOG4602, VOG9667, VOG3490, VOG0189, VOG6875, VOG4861, VOG5447, VOG0796, VOG10227, VOG4773, VOG4685, VOG0749, VOG3890, VOG3891, VOG3892, VOG6163, VOG4865, VOG4885, VOG3894, VOG4550, VOG4691, VOG4862, VOG7718, VOG4705 |
Phigaro v2.4.0 (Database: Jan. 2024 release)