Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 12 | 10 | 9 | 18.03 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| PLIMGHLO_88 | 85451 | 88021 | Bacillus subtilis mprF | ARO:3003324 | Bsub_mprF | 99.77 | 100.00 | AL009126.3 |
| PLIMGHLO_52 | 84664 | 85002 | ykkC | ARO:3003063 | ykkC | 98.21 | 100.00 | AL009126.1 |
| PLIMGHLO_52 | 84347 | 84664 | ykkD | ARO:3003064 | ykkD | 98.10 | 100.00 | AL009126.1 |
| PLIMGHLO_13 | 65065 | 65916 | aadK | ARO:3002627 | aadK | 98.24 | 99.65 | AL009126.1 |
| PLIMGHLO_61 | 34815 | 35735 | mphK | ARO:3004541 | mphK | 98.68 | 100.00 | CP010314.1 |
| PLIMGHLO_26 | 26151 | 26744 | tmrB | ARO:3003059 | tmrB | 100.00 | 100.00 | AL009126.3 |
| PLIMGHLO_82 | 21106 | 22539 | lmrB | ARO:3002813 | lmrB | 99.16 | 100.00 | JYFL01000006.1 |
| PLIMGHLO_64 | 17779 | 18948 | bmr | ARO:3003007 | bmr | 99.49 | 100.00 | M33768.1 |
| PLIMGHLO_43 | 12934 | 14577 | vmlR | ARO:3004476 | vmlR | 98.32 | 100.00 | NC_000964.3 |
| PLIMGHLO_103 | 6846 | 8048 | blt | ARO:3003006 | blt | 99.25 | 100.00 | L32599.1 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| PLIMGHLO_13 | 65065 | 65916 | aadK | Streptomycin | 98.48 | 99.65 | M26879 |
| PLIMGHLO_61 | 34815 | 35735 | mph(K) | Spiramycin, Telithromycin | 98.05 | 100.00 | NC_000964 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| PLIMGHLO_61 | 34815 | 35723 | mphK | macrolide 2'-phosphotransferase MphK | BLASTX | 98.68 | 99.02 | WP_003246254.1 |
| PLIMGHLO_13 | 65065 | 65913 | aadK | aminoglycoside 6-adenylyltransferase AadK | BLASTX | 98.24 | 100.00 | WP_003229862.1 |
| PLIMGHLO_43 | 12937 | 14577 | vmlR | ABC-F type ribosomal protection protein VmlR | BLASTX | 96.71 | 99.82 | WP_003234144.1 |
| PLIMGHLO_27 | 47020 | 47412 | satA | streptothricin N-acetyltransferase SatA | PARTIALX | 91.60 | 75.72 | WP_003242546.1 |
| PLIMGHLO_106 | 1452 | 4049 | rphC | rifamycin-inactivating phosphotransferase RphC | BLASTX | 80.48 | 100.00 | WP_087347987.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| PLIMGHLO_113 | 798 | 1979 | capB | 0.0 | Capsule | Immune modulation | 99.66 | 100.00 | NP_391471 |
| PLIMGHLO_7 | 107829 | 108614 | dhbA | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.36 | 100.00 | NP_391080 |
| PLIMGHLO_113 | 2462 | 3604 | capA | 0.0 | Capsule | Immune modulation | 99.30 | 100.00 | NP_391469 |
| PLIMGHLO_44 | 50003 | 51766 | dep/capD | 0.0 | Capsule | Immune modulation | 98.98 | 100.00 | NP_389723 |
| PLIMGHLO_7 | 104004 | 104942 | dhbB | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.94 | 100.00 | NP_391077 |
| PLIMGHLO_31 | 39473 | 40114 | hlyIII | 0.0 | Hemolysin III | Exotoxin | 98.91 | 100.00 | NP_390062 |
| PLIMGHLO_113 | 1994 | 2443 | capC | 0.0 | Capsule | Immune modulation | 98.89 | 100.00 | NP_391470 |
| PLIMGHLO_7 | 106607 | 107803 | dhbC | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.75 | 100.00 | NP_391079 |
| PLIMGHLO_7 | 96848 | 103984 | dhbF | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.56 | 100.00 | NP_391076 |
| PLIMGHLO_7 | 104970 | 106589 | dhbE | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 97.28 | 100.00 | NP_391078 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| PLIMGHLO_90 | 28136 | 27000 | tufA | 0.0 | elongation factor Tu | 85.75 | 95.69 | AHM69299 |
| PLIMGHLO_17 | 11336 | 10467 | pdxS | 1.12e-151 | encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS | 83.79 | 98.31 | ACE61268 |
| PLIMGHLO_6 | 73981 | 74757 | CodY | 1.27e-125 | nutrient-responsive regulator | 81.08 | 100.00 | ACJ80679 |
| PLIMGHLO_24 | 81153 | 81722 | ClpP | 7.58e-100 | part of proteolytic complex | 81.05 | 97.44 | BAB94595 |
| PLIMGHLO_32 | 41487 | 41095 | spxA1 | 4.13e-71 | redox-responsive transcription factor | 83.97 | 100.00 | AAF21893 |
| PLIMGHLO_32 | 41487 | 41095 | spx | 2.46e-69 | essential gene which is a thiol/oxidative stress sensor | 80.15 | 100.00 | BAB57159 |
| PLIMGHLO_58 | 20678 | 20920 | SpoVG | 6.46e-39 | RNA-binding protein | 81.48 | 79.41 | AAT02994 |
| PLIMGHLO_49 | 1923 | 1726 | CspA | 3.37e-32 | cold shock protein | 90.91 | 100.00 | CAA62903 |
| PLIMGHLO_31 | 51345 | 51539 | CspD | 7.94e-29 | cold shock protein | 81.54 | 98.48 | CAC99957 |
| PLIMGHLO_36 | 30202 | 30008 | CspB | 7.30e-28 | cold shock protein | 83.08 | 98.48 | CAD00094 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| PLIMGHLO_1 | 0.0 | 1.0 | 0.0 |
| PLIMGHLO_3 | 0.0 | 1.0 | 0.0 |
| PLIMGHLO_4 | 0.0 | 1.0 | 0.0 |
| PLIMGHLO_5 | 0.0 | 1.0 | 0.0 |
| PLIMGHLO_6 | 0.0 | 1.0 | 0.0 |
| PLIMGHLO_7 | 0.0 | 1.0 | 0.0 |
| PLIMGHLO_8 | 0.0 | 1.0 | 0.0 |
| PLIMGHLO_9 | 0.0 | 1.0 | 0.0 |
| PLIMGHLO_10 | 0.0 | 1.0 | 0.0 |
| PLIMGHLO_11 | 0.0 | 1.0 | 0.0 |
| PLIMGHLO_12 | 0.0 | 1.0 | 0.0 |
| PLIMGHLO_13 | 0.0 | 1.0 | 0.0 |
| PLIMGHLO_14 | 0.0 | 1.0 | 0.0 |
| PLIMGHLO_15 | 0.0 | 1.0 | 0.0 |
| PLIMGHLO_16 | 0.0 | 1.0 | 0.0 |
| PLIMGHLO_17 | 0.0 | 1.0 | 0.0 |
| PLIMGHLO_18 | 0.0 | 1.0 | 0.0 |
| PLIMGHLO_19 | 0.0 | 1.0 | 0.0 |
| PLIMGHLO_20 | 0.0 | 1.0 | 0.0 |
| PLIMGHLO_21 | 0.0 | 1.0 | 0.0 |
| PLIMGHLO_22 | 0.0 | 1.0 | 0.0 |
| PLIMGHLO_24 | 0.0 | 1.0 | 0.0 |
| PLIMGHLO_25 | 0.0 | 1.0 | 0.0 |
| PLIMGHLO_26 | 0.0 | 1.0 | 0.0 |
| PLIMGHLO_27 | 0.0 | 1.0 | 0.0 |
| PLIMGHLO_29 | 0.0 | 1.0 | 0.0 |
| PLIMGHLO_31 | 0.0 | 1.0 | 0.0 |
| PLIMGHLO_32 | 0.0 | 1.0 | 0.0 |
| PLIMGHLO_33 | 0.0 | 1.0 | 0.0 |
| PLIMGHLO_34 | 0.0 | 1.0 | 0.0 |
| PLIMGHLO_35 | 0.0 | 1.0 | 0.0 |
| PLIMGHLO_36 | 0.0 | 1.0 | 0.0 |
| PLIMGHLO_37 | 1.0 | 0.0 | 1.0 |
| PLIMGHLO_38 | 0.0 | 1.0 | 0.0 |
| PLIMGHLO_39 | 0.0 | 1.0 | 0.0 |
| PLIMGHLO_40 | 0.0 | 1.0 | 0.0 |
| PLIMGHLO_41 | 0.0 | 1.0 | 0.0 |
| PLIMGHLO_42 | 0.0 | 1.0 | 0.0 |
| PLIMGHLO_43 | 0.0 | 1.0 | 0.0 |
| PLIMGHLO_44 | 0.0 | 1.0 | 0.0 |
| PLIMGHLO_45 | 0.0 | 1.0 | 0.0 |
| PLIMGHLO_46 | 0.0 | 1.0 | 0.0 |
| PLIMGHLO_47 | 0.0 | 1.0 | 0.0 |
| PLIMGHLO_48 | 0.0 | 1.0 | 0.0 |
| PLIMGHLO_49 | 0.0 | 1.0 | 0.0 |
| PLIMGHLO_50 | 0.0 | 1.0 | 0.0 |
| PLIMGHLO_51 | 0.0 | 1.0 | 0.0 |
| PLIMGHLO_52 | 0.0 | 1.0 | 0.0 |
| PLIMGHLO_53 | 0.0 | 1.0 | 0.0 |
| PLIMGHLO_54 | 0.0 | 1.0 | 0.0 |
| PLIMGHLO_55 | 0.0 | 1.0 | 0.0 |
| PLIMGHLO_56 | 0.0 | 1.0 | 0.0 |
| PLIMGHLO_57 | 0.0 | 1.0 | 0.0 |
| PLIMGHLO_58 | 0.0 | 1.0 | 0.0 |
| PLIMGHLO_59 | 0.0 | 1.0 | 0.0 |
| PLIMGHLO_60 | 0.0 | 1.0 | 0.0 |
| PLIMGHLO_61 | 0.0 | 1.0 | 0.0 |
| PLIMGHLO_62 | 0.0 | 1.0 | 0.0 |
| PLIMGHLO_63 | 0.0 | 1.0 | 0.0 |
| PLIMGHLO_64 | 0.0 | 1.0 | 0.0 |
| PLIMGHLO_65 | 0.0 | 1.0 | 0.0 |
| PLIMGHLO_66 | 1.0 | 0.0 | 1.0 |
| PLIMGHLO_67 | 1.0 | 0.0 | 1.0 |
| PLIMGHLO_68 | 0.0 | 1.0 | 0.0 |
| PLIMGHLO_69 | 0.0 | 1.0 | 0.0 |
| PLIMGHLO_70 | 0.0 | 1.0 | 0.0 |
| PLIMGHLO_71 | 0.0 | 1.0 | 0.0 |
| PLIMGHLO_72 | 0.0 | 1.0 | 0.0 |
| PLIMGHLO_73 | 0.0 | 1.0 | 0.0 |
| PLIMGHLO_74 | 0.0 | 1.0 | 0.0 |
| PLIMGHLO_75 | 0.0 | 1.0 | 0.0 |
| PLIMGHLO_76 | 0.0 | 1.0 | 0.0 |
| PLIMGHLO_77 | 0.0 | 1.0 | 0.0 |
| PLIMGHLO_78 | 0.0 | 1.0 | 0.0 |
| PLIMGHLO_79 | 0.0 | 1.0 | 0.0 |
| PLIMGHLO_80 | 0.0 | 1.0 | 0.0 |
| PLIMGHLO_82 | 0.0 | 1.0 | 0.0 |
| PLIMGHLO_83 | 0.0 | 1.0 | 0.0 |
| PLIMGHLO_84 | 0.0 | 1.0 | 0.0 |
| PLIMGHLO_85 | 0.0 | 1.0 | 0.0 |
| PLIMGHLO_86 | 0.0 | 1.0 | 0.0 |
| PLIMGHLO_87 | 0.0 | 1.0 | 0.0 |
| PLIMGHLO_88 | 0.0 | 1.0 | 0.0 |
| PLIMGHLO_89 | 0.0 | 1.0 | 0.0 |
| PLIMGHLO_90 | 0.0 | 1.0 | 0.0 |
| PLIMGHLO_91 | 0.0 | 1.0 | 0.0 |
| PLIMGHLO_92 | 0.0 | 1.0 | 0.0 |
| PLIMGHLO_93 | 0.0 | 1.0 | 0.0 |
| PLIMGHLO_94 | 0.0 | 1.0 | 0.0 |
| PLIMGHLO_95 | 0.0 | 1.0 | 0.0 |
| PLIMGHLO_96 | 0.0 | 1.0 | 0.0 |
| PLIMGHLO_97 | 0.0 | 1.0 | 0.0 |
| PLIMGHLO_98 | 0.0 | 1.0 | 0.0 |
| PLIMGHLO_99 | 0.0 | 1.0 | 0.0 |
| PLIMGHLO_100 | 0.0 | 1.0 | 0.0 |
| PLIMGHLO_101 | 0.0 | 1.0 | 0.0 |
| PLIMGHLO_102 | 1.0 | 0.0 | 1.0 |
| PLIMGHLO_103 | 0.0 | 1.0 | 0.0 |
| PLIMGHLO_104 | 0.0 | 1.0 | 0.0 |
| PLIMGHLO_105 | 0.0 | 1.0 | 0.0 |
| PLIMGHLO_106 | 0.0 | 1.0 | 0.0 |
| PLIMGHLO_107 | 0.0 | 1.0 | 0.0 |
| PLIMGHLO_108 | 1.0 | 0.0 | 1.0 |
| PLIMGHLO_109 | 0.0 | 1.0 | 0.0 |
| PLIMGHLO_111 | 0.0 | 1.0 | 0.0 |
| PLIMGHLO_113 | 0.0 | 1.0 | 0.0 |
| PLIMGHLO_114 | 0.0 | 1.0 | 0.0 |
| PLIMGHLO_117 | 0.0 | 1.0 | 0.0 |
| PLIMGHLO_118 | 0.0 | 1.0 | 0.0 |
| PLIMGHLO_119 | 0.0 | 1.0 | 0.0 |
| PLIMGHLO_120 | 0.0 | 1.0 | 0.0 |
| PLIMGHLO_121 | 0.0 | 1.0 | 0.0 |
| PLIMGHLO_122 | 0.0 | 1.0 | 0.0 |
| PLIMGHLO_125 | 0.0 | 1.0 | 0.0 |
| PLIMGHLO_126 | 0.0 | 1.0 | 0.0 |
| PLIMGHLO_129 | 0.0 | 1.0 | 0.0 |
| PLIMGHLO_130 | 0.0 | 1.0 | 0.0 |
| PLIMGHLO_131 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| PLIMGHLO_42 | 10178 | 42621 | False | Siphoviridae | VOG6513, VOG0275, VOG4602, VOG9667, VOG9667, VOG0650, VOG4552, VOG4603, VOG8233, VOG0186, VOG4566, VOG0189, VOG4693, VOG1985, VOG0198, VOG0713, VOG4841, VOG4581, VOG1886, VOG0202, VOG4556, VOG4573, VOG4553, VOG5361, VOG0204, VOG4589, VOG0539, VOG0704, VOG4586, VOG0541, VOG5910, VOG6163, VOG0801 |
| PLIMGHLO_52 | 114466 | 141908 | False | Myoviridae | VOG0753, VOG4705, VOG7718, VOG4862, VOG4691, VOG4550, VOG3894, VOG4885, VOG4865, VOG6163, VOG3892, VOG3891, VOG3890, VOG0749, VOG4685, VOG4773, VOG10227, VOG0796, VOG5447, VOG4861, VOG6875, VOG0189, VOG3490, VOG9667 |
Phigaro v2.4.0 (Database: Jan. 2024 release)