Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 12 | 10 | 9 | 18.03 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| CCFHKELF_15 | 85337 | 87907 | Bacillus subtilis mprF | ARO:3003324 | Bsub_mprF | 99.77 | 100.00 | AL009126.3 |
| CCFHKELF_96 | 65099 | 65950 | aadK | ARO:3002627 | aadK | 98.24 | 99.65 | AL009126.1 |
| CCFHKELF_11 | 62385 | 62702 | ykkD | ARO:3003064 | ykkD | 98.10 | 100.00 | AL009126.1 |
| CCFHKELF_11 | 62047 | 62385 | ykkC | ARO:3003063 | ykkC | 98.21 | 100.00 | AL009126.1 |
| CCFHKELF_77 | 36632 | 37801 | bmr | ARO:3003007 | bmr | 99.49 | 100.00 | M33768.1 |
| CCFHKELF_18 | 26368 | 26961 | tmrB | ARO:3003059 | tmrB | 100.00 | 100.00 | AL009126.3 |
| CCFHKELF_122 | 15981 | 16901 | mphK | ARO:3004541 | mphK | 98.68 | 100.00 | CP010314.1 |
| CCFHKELF_83 | 13003 | 14646 | vmlR | ARO:3004476 | vmlR | 98.32 | 100.00 | NC_000964.3 |
| CCFHKELF_62 | 7479 | 8912 | lmrB | ARO:3002813 | lmrB | 99.16 | 100.00 | JYFL01000006.1 |
| CCFHKELF_98 | 800 | 2002 | blt | ARO:3003006 | blt | 99.25 | 100.00 | L32599.1 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| CCFHKELF_96 | 65099 | 65950 | aadK | Streptomycin | 98.48 | 99.65 | M26879 |
| CCFHKELF_122 | 15981 | 16901 | mph(K) | Spiramycin, Telithromycin | 98.05 | 100.00 | NC_000964 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| CCFHKELF_122 | 15981 | 16889 | mphK | macrolide 2'-phosphotransferase MphK | BLASTX | 98.68 | 99.02 | WP_003246254.1 |
| CCFHKELF_96 | 65099 | 65947 | aadK | aminoglycoside 6-adenylyltransferase AadK | BLASTX | 98.24 | 100.00 | WP_003229862.1 |
| CCFHKELF_83 | 13006 | 14646 | vmlR | ABC-F type ribosomal protection protein VmlR | BLASTX | 96.71 | 99.82 | WP_003234144.1 |
| CCFHKELF_41 | 61874 | 62266 | satA | streptothricin N-acetyltransferase SatA | PARTIALX | 91.60 | 75.72 | WP_003242546.1 |
| CCFHKELF_79 | 1447 | 4044 | rphC | rifamycin-inactivating phosphotransferase RphC | BLASTX | 80.48 | 100.00 | WP_087347987.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| CCFHKELF_125 | 1893 | 3074 | capB | 0.0 | Capsule | Immune modulation | 99.66 | 100.00 | NP_391471 |
| CCFHKELF_4 | 107777 | 108562 | dhbA | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.36 | 100.00 | NP_391080 |
| CCFHKELF_125 | 268 | 1410 | capA | 0.0 | Capsule | Immune modulation | 99.30 | 100.00 | NP_391469 |
| CCFHKELF_14 | 53662 | 55425 | dep/capD | 0.0 | Capsule | Immune modulation | 98.98 | 100.00 | NP_389723 |
| CCFHKELF_4 | 103952 | 104890 | dhbB | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.94 | 100.00 | NP_391077 |
| CCFHKELF_1 | 89051 | 89692 | hlyIII | 0.0 | Hemolysin III | Exotoxin | 98.91 | 100.00 | NP_390062 |
| CCFHKELF_125 | 1429 | 1878 | capC | 0.0 | Capsule | Immune modulation | 98.89 | 100.00 | NP_391470 |
| CCFHKELF_4 | 106555 | 107751 | dhbC | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.75 | 100.00 | NP_391079 |
| CCFHKELF_4 | 96796 | 103932 | dhbF | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.56 | 100.00 | NP_391076 |
| CCFHKELF_4 | 104918 | 106537 | dhbE | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 97.28 | 100.00 | NP_391078 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| CCFHKELF_6 | 28065 | 26929 | tufA | 0.0 | elongation factor Tu | 85.75 | 95.69 | AHM69299 |
| CCFHKELF_128 | 4442 | 5311 | pdxS | 1.12e-151 | encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS | 83.79 | 98.31 | ACE61268 |
| CCFHKELF_60 | 82845 | 82069 | CodY | 1.27e-125 | nutrient-responsive regulator | 81.08 | 100.00 | ACJ80679 |
| CCFHKELF_75 | 81258 | 81827 | ClpP | 7.09e-100 | part of proteolytic complex | 81.05 | 97.44 | BAB94595 |
| CCFHKELF_17 | 41544 | 41152 | spxA1 | 4.13e-71 | redox-responsive transcription factor | 83.97 | 100.00 | AAF21893 |
| CCFHKELF_17 | 41544 | 41152 | spx | 2.46e-69 | essential gene which is a thiol/oxidative stress sensor | 80.15 | 100.00 | BAB57159 |
| CCFHKELF_22 | 20578 | 20820 | SpoVG | 6.47e-39 | RNA-binding protein | 81.48 | 79.41 | AAT02994 |
| CCFHKELF_26 | 75921 | 76118 | CspA | 3.36e-32 | cold shock protein | 90.91 | 100.00 | CAA62903 |
| CCFHKELF_1 | 77820 | 77626 | CspD | 1.01e-28 | cold shock protein | 81.54 | 98.48 | CAC99957 |
| CCFHKELF_52 | 49445 | 49639 | CspB | 7.31e-28 | cold shock protein | 83.08 | 98.48 | CAD00094 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| CCFHKELF_50 | 6946 | 7440 | rep21 | 495 / 717 | 90.51 | FR821780 |
| CCFHKELF_50 | 5150 | 5314 | Col(SD853) | 165 / 194 | 86.06 | NC015392 |
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| CCFHKELF_1 | 0.0 | 1.0 | 0.0 |
| CCFHKELF_2 | 0.0 | 1.0 | 0.0 |
| CCFHKELF_3 | 0.0 | 1.0 | 0.0 |
| CCFHKELF_4 | 0.0 | 1.0 | 0.0 |
| CCFHKELF_5 | 0.0 | 1.0 | 0.0 |
| CCFHKELF_6 | 0.0 | 1.0 | 0.0 |
| CCFHKELF_7 | 0.0 | 1.0 | 0.0 |
| CCFHKELF_10 | 0.0 | 1.0 | 0.0 |
| CCFHKELF_11 | 0.0 | 1.0 | 0.0 |
| CCFHKELF_12 | 0.0 | 1.0 | 0.0 |
| CCFHKELF_13 | 0.0 | 1.0 | 0.0 |
| CCFHKELF_14 | 0.0 | 1.0 | 0.0 |
| CCFHKELF_15 | 0.0 | 1.0 | 0.0 |
| CCFHKELF_16 | 0.0 | 1.0 | 0.0 |
| CCFHKELF_17 | 0.0 | 1.0 | 0.0 |
| CCFHKELF_18 | 0.0 | 1.0 | 0.0 |
| CCFHKELF_19 | 0.0 | 1.0 | 0.0 |
| CCFHKELF_20 | 0.0 | 1.0 | 0.0 |
| CCFHKELF_21 | 0.0 | 1.0 | 0.0 |
| CCFHKELF_22 | 0.0 | 1.0 | 0.0 |
| CCFHKELF_23 | 0.0 | 1.0 | 0.0 |
| CCFHKELF_24 | 0.0 | 1.0 | 0.0 |
| CCFHKELF_25 | 0.0 | 1.0 | 0.0 |
| CCFHKELF_26 | 0.0 | 1.0 | 0.0 |
| CCFHKELF_27 | 0.0 | 1.0 | 0.0 |
| CCFHKELF_28 | 1.0 | 0.0 | 1.0 |
| CCFHKELF_29 | 1.0 | 0.0 | 1.0 |
| CCFHKELF_30 | 0.0 | 1.0 | 0.0 |
| CCFHKELF_31 | 0.0 | 1.0 | 0.0 |
| CCFHKELF_32 | 0.0 | 1.0 | 0.0 |
| CCFHKELF_33 | 0.0 | 1.0 | 0.0 |
| CCFHKELF_34 | 0.0 | 1.0 | 0.0 |
| CCFHKELF_35 | 0.0 | 1.0 | 0.0 |
| CCFHKELF_36 | 0.0 | 1.0 | 0.0 |
| CCFHKELF_37 | 0.0 | 1.0 | 0.0 |
| CCFHKELF_38 | 0.0 | 1.0 | 0.0 |
| CCFHKELF_39 | 0.0 | 1.0 | 0.0 |
| CCFHKELF_40 | 0.0 | 1.0 | 0.0 |
| CCFHKELF_41 | 0.0 | 1.0 | 0.0 |
| CCFHKELF_42 | 0.0 | 1.0 | 0.0 |
| CCFHKELF_43 | 0.0 | 1.0 | 0.0 |
| CCFHKELF_44 | 0.0 | 1.0 | 0.0 |
| CCFHKELF_45 | 0.0 | 1.0 | 0.0 |
| CCFHKELF_46 | 0.0 | 1.0 | 0.0 |
| CCFHKELF_47 | 0.0 | 1.0 | 0.0 |
| CCFHKELF_48 | 1.0 | 0.0 | 1.0 |
| CCFHKELF_49 | 0.0 | 1.0 | 0.0 |
| CCFHKELF_50 | 1.0 | 0.0 | 1.0 |
| CCFHKELF_51 | 0.0 | 1.0 | 0.0 |
| CCFHKELF_52 | 0.0 | 1.0 | 0.0 |
| CCFHKELF_53 | 0.0 | 1.0 | 0.0 |
| CCFHKELF_56 | 0.0 | 1.0 | 0.0 |
| CCFHKELF_57 | 1.0 | 0.0 | 1.0 |
| CCFHKELF_58 | 0.0 | 1.0 | 0.0 |
| CCFHKELF_59 | 0.0 | 1.0 | 0.0 |
| CCFHKELF_60 | 0.0 | 1.0 | 0.0 |
| CCFHKELF_61 | 0.0 | 1.0 | 0.0 |
| CCFHKELF_62 | 0.0 | 1.0 | 0.0 |
| CCFHKELF_63 | 0.0 | 1.0 | 0.0 |
| CCFHKELF_64 | 0.0 | 1.0 | 0.0 |
| CCFHKELF_65 | 0.0 | 1.0 | 0.0 |
| CCFHKELF_66 | 1.0 | 0.0 | 1.0 |
| CCFHKELF_67 | 0.0 | 1.0 | 0.0 |
| CCFHKELF_68 | 0.0 | 1.0 | 0.0 |
| CCFHKELF_69 | 0.0 | 1.0 | 0.0 |
| CCFHKELF_70 | 0.0 | 1.0 | 0.0 |
| CCFHKELF_71 | 1.0 | 0.0 | 1.0 |
| CCFHKELF_72 | 0.0 | 1.0 | 0.0 |
| CCFHKELF_73 | 0.0 | 1.0 | 0.0 |
| CCFHKELF_74 | 0.0 | 1.0 | 0.0 |
| CCFHKELF_75 | 0.0 | 1.0 | 0.0 |
| CCFHKELF_76 | 1.0 | 0.0 | 1.0 |
| CCFHKELF_77 | 0.0 | 1.0 | 0.0 |
| CCFHKELF_78 | 0.0 | 1.0 | 0.0 |
| CCFHKELF_79 | 0.0 | 1.0 | 0.0 |
| CCFHKELF_80 | 0.0 | 1.0 | 0.0 |
| CCFHKELF_81 | 0.0 | 1.0 | 0.0 |
| CCFHKELF_82 | 0.0 | 1.0 | 0.0 |
| CCFHKELF_83 | 0.0 | 1.0 | 0.0 |
| CCFHKELF_84 | 0.0 | 1.0 | 0.0 |
| CCFHKELF_85 | 0.0 | 1.0 | 0.0 |
| CCFHKELF_86 | 0.0 | 1.0 | 0.0 |
| CCFHKELF_87 | 0.0 | 1.0 | 0.0 |
| CCFHKELF_88 | 0.0 | 1.0 | 0.0 |
| CCFHKELF_89 | 0.0 | 1.0 | 0.0 |
| CCFHKELF_90 | 0.0 | 1.0 | 0.0 |
| CCFHKELF_91 | 0.0 | 1.0 | 0.0 |
| CCFHKELF_92 | 0.0 | 1.0 | 0.0 |
| CCFHKELF_93 | 1.0 | 0.0 | 1.0 |
| CCFHKELF_94 | 0.0 | 1.0 | 0.0 |
| CCFHKELF_96 | 0.0 | 1.0 | 0.0 |
| CCFHKELF_97 | 0.0 | 1.0 | 0.0 |
| CCFHKELF_98 | 0.0 | 1.0 | 0.0 |
| CCFHKELF_99 | 1.0 | 0.0 | 1.0 |
| CCFHKELF_100 | 0.0 | 1.0 | 0.0 |
| CCFHKELF_102 | 0.0 | 1.0 | 0.0 |
| CCFHKELF_103 | 0.0 | 1.0 | 0.0 |
| CCFHKELF_105 | 0.0 | 1.0 | 0.0 |
| CCFHKELF_106 | 0.0 | 1.0 | 0.0 |
| CCFHKELF_107 | 1.0 | 0.0 | 1.0 |
| CCFHKELF_108 | 1.0 | 0.0 | 1.0 |
| CCFHKELF_109 | 0.0 | 1.0 | 0.0 |
| CCFHKELF_110 | 0.0 | 1.0 | 0.0 |
| CCFHKELF_111 | 0.0 | 1.0 | 0.0 |
| CCFHKELF_112 | 0.0 | 1.0 | 0.0 |
| CCFHKELF_113 | 0.0 | 1.0 | 0.0 |
| CCFHKELF_114 | 0.0 | 1.0 | 0.0 |
| CCFHKELF_115 | 0.0 | 1.0 | 0.0 |
| CCFHKELF_116 | 0.0 | 1.0 | 0.0 |
| CCFHKELF_117 | 0.0 | 1.0 | 0.0 |
| CCFHKELF_118 | 0.0 | 1.0 | 0.0 |
| CCFHKELF_119 | 0.0 | 1.0 | 0.0 |
| CCFHKELF_120 | 0.0 | 1.0 | 0.0 |
| CCFHKELF_121 | 0.0 | 1.0 | 0.0 |
| CCFHKELF_122 | 0.0 | 1.0 | 0.0 |
| CCFHKELF_123 | 0.0 | 1.0 | 0.0 |
| CCFHKELF_124 | 0.0 | 1.0 | 0.0 |
| CCFHKELF_125 | 0.0 | 1.0 | 0.0 |
| CCFHKELF_126 | 0.0 | 1.0 | 0.0 |
| CCFHKELF_127 | 0.0 | 1.0 | 0.0 |
| CCFHKELF_128 | 0.0 | 1.0 | 0.0 |
| CCFHKELF_129 | 1.0 | 0.0 | 1.0 |
| CCFHKELF_130 | 0.0 | 1.0 | 0.0 |
| CCFHKELF_131 | 0.0 | 1.0 | 0.0 |
| CCFHKELF_132 | 0.0 | 1.0 | 0.0 |
| CCFHKELF_133 | 1.0 | 0.0 | 1.0 |
| CCFHKELF_134 | 0.0 | 1.0 | 0.0 |
| CCFHKELF_135 | 0.0 | 1.0 | 0.0 |
| CCFHKELF_137 | 0.0 | 1.0 | 0.0 |
| CCFHKELF_140 | 0.0 | 1.0 | 0.0 |
| CCFHKELF_141 | 0.0 | 1.0 | 0.0 |
| CCFHKELF_142 | 0.0 | 1.0 | 0.0 |
| CCFHKELF_147 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| CCFHKELF_11 | 6420 | 32583 | False | Myoviridae | VOG3490, VOG0189, VOG4861, VOG5447, VOG0796, VOG10227, VOG4773, VOG4685, VOG0749, VOG3890, VOG3891, VOG3892, VOG6163, VOG4865, VOG4885, VOG3894, VOG4550, VOG4691, VOG4862, VOG7718, VOG4705 |
| CCFHKELF_42 | 10457 | 42900 | False | Siphoviridae | VOG6513, VOG0275, VOG4602, VOG9667, VOG9667, VOG0650, VOG4552, VOG4603, VOG8233, VOG0186, VOG4566, VOG0189, VOG4693, VOG1985, VOG0198, VOG0713, VOG4841, VOG4581, VOG1886, VOG0202, VOG4556, VOG4573, VOG4553, VOG5361, VOG0204, VOG4589, VOG0539, VOG0704, VOG4586, VOG0541, VOG5910, VOG6163, VOG0801 |
Phigaro v2.4.0 (Database: Jan. 2024 release)