Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 12 | 10 | 9 | 18.03 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| MOHGGCOF_48 | 85369 | 87939 | Bacillus subtilis mprF | ARO:3003324 | Bsub_mprF | 99.77 | 100.00 | AL009126.3 |
| MOHGGCOF_1 | 84828 | 85166 | ykkC | ARO:3003063 | ykkC | 98.21 | 100.00 | AL009126.1 |
| MOHGGCOF_1 | 84511 | 84828 | ykkD | ARO:3003064 | ykkD | 98.10 | 100.00 | AL009126.1 |
| MOHGGCOF_47 | 46431 | 47024 | tmrB | ARO:3003059 | tmrB | 100.00 | 100.00 | AL009126.3 |
| MOHGGCOF_4 | 36688 | 37857 | bmr | ARO:3003007 | bmr | 99.49 | 100.00 | M33768.1 |
| MOHGGCOF_64 | 28777 | 30420 | vmlR | ARO:3004476 | vmlR | 98.32 | 100.00 | NC_000964.3 |
| MOHGGCOF_28 | 7704 | 8555 | aadK | ARO:3002627 | aadK | 98.24 | 99.65 | AL009126.1 |
| MOHGGCOF_106 | 7010 | 8212 | blt | ARO:3003006 | blt | 99.25 | 100.00 | L32599.1 |
| MOHGGCOF_8 | 6338 | 7258 | mphK | ARO:3004541 | mphK | 98.68 | 100.00 | CP010314.1 |
| MOHGGCOF_97 | 5890 | 7329 | lmrB | ARO:3002813 | lmrB | 98.95 | 100.42 | JYFL01000006.1 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| MOHGGCOF_28 | 7704 | 8555 | aadK | Streptomycin | 98.48 | 99.65 | M26879 |
| MOHGGCOF_8 | 6338 | 7258 | mph(K) | Spiramycin, Telithromycin | 98.05 | 100.00 | NC_000964 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| MOHGGCOF_8 | 6350 | 7258 | mphK | macrolide 2'-phosphotransferase MphK | BLASTX | 98.68 | 99.02 | WP_003246254.1 |
| MOHGGCOF_28 | 7707 | 8555 | aadK | aminoglycoside 6-adenylyltransferase AadK | BLASTX | 98.24 | 100.00 | WP_003229862.1 |
| MOHGGCOF_64 | 28777 | 30417 | vmlR | ABC-F type ribosomal protection protein VmlR | BLASTX | 96.71 | 99.82 | WP_003234144.1 |
| MOHGGCOF_9 | 17248 | 17640 | satA | streptothricin N-acetyltransferase SatA | PARTIALX | 91.60 | 75.72 | WP_003242546.1 |
| MOHGGCOF_120 | 1500 | 4097 | rphC | rifamycin-inactivating phosphotransferase RphC | BLASTX | 80.48 | 100.00 | WP_087347987.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| MOHGGCOF_101 | 2044 | 3225 | capB | 0.0 | Capsule | Immune modulation | 99.66 | 100.00 | NP_391471 |
| MOHGGCOF_27 | 107780 | 108565 | dhbA | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.36 | 100.00 | NP_391080 |
| MOHGGCOF_101 | 419 | 1561 | capA | 0.0 | Capsule | Immune modulation | 99.30 | 100.00 | NP_391469 |
| MOHGGCOF_17 | 53602 | 55365 | dep/capD | 0.0 | Capsule | Immune modulation | 98.98 | 100.00 | NP_389723 |
| MOHGGCOF_27 | 103955 | 104893 | dhbB | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.94 | 100.00 | NP_391077 |
| MOHGGCOF_5 | 89054 | 89695 | hlyIII | 0.0 | Hemolysin III | Exotoxin | 98.91 | 100.00 | NP_390062 |
| MOHGGCOF_101 | 1580 | 2029 | capC | 0.0 | Capsule | Immune modulation | 98.89 | 100.00 | NP_391470 |
| MOHGGCOF_27 | 106558 | 107754 | dhbC | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.75 | 100.00 | NP_391079 |
| MOHGGCOF_27 | 96799 | 103935 | dhbF | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.56 | 100.00 | NP_391076 |
| MOHGGCOF_27 | 104921 | 106540 | dhbE | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 97.28 | 100.00 | NP_391078 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| MOHGGCOF_16 | 27935 | 26799 | tufA | 0.0 | elongation factor Tu | 85.75 | 95.69 | AHM69299 |
| MOHGGCOF_87 | 11315 | 10446 | pdxS | 1.13e-151 | encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS | 83.79 | 98.31 | ACE61268 |
| MOHGGCOF_33 | 74173 | 74949 | CodY | 1.27e-125 | nutrient-responsive regulator | 81.08 | 100.00 | ACJ80679 |
| MOHGGCOF_14 | 45257 | 44688 | ClpP | 7.59e-100 | part of proteolytic complex | 81.05 | 97.44 | BAB94595 |
| MOHGGCOF_66 | 41406 | 41014 | spxA1 | 4.11e-71 | redox-responsive transcription factor | 83.97 | 100.00 | AAF21893 |
| MOHGGCOF_66 | 41406 | 41014 | spx | 2.45e-69 | essential gene which is a thiol/oxidative stress sensor | 80.15 | 100.00 | BAB57159 |
| MOHGGCOF_22 | 20501 | 20743 | SpoVG | 6.46e-39 | RNA-binding protein | 81.48 | 79.41 | AAT02994 |
| MOHGGCOF_53 | 1923 | 1726 | CspA | 9.21e-33 | cold shock protein | 90.91 | 100.00 | CAA62903 |
| MOHGGCOF_5 | 77823 | 77629 | CspD | 7.94e-29 | cold shock protein | 81.54 | 98.48 | CAC99957 |
| MOHGGCOF_21 | 30231 | 30037 | CspB | 7.31e-28 | cold shock protein | 83.08 | 98.48 | CAD00094 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| MOHGGCOF_1 | 0.0 | 1.0 | 0.0 |
| MOHGGCOF_2 | 0.0 | 1.0 | 0.0 |
| MOHGGCOF_3 | 0.0 | 1.0 | 0.0 |
| MOHGGCOF_4 | 0.0 | 1.0 | 0.0 |
| MOHGGCOF_5 | 0.0 | 1.0 | 0.0 |
| MOHGGCOF_6 | 0.0 | 1.0 | 0.0 |
| MOHGGCOF_7 | 0.0 | 1.0 | 0.0 |
| MOHGGCOF_8 | 0.0 | 1.0 | 0.0 |
| MOHGGCOF_9 | 0.0 | 1.0 | 0.0 |
| MOHGGCOF_10 | 0.0 | 1.0 | 0.0 |
| MOHGGCOF_11 | 0.0 | 1.0 | 0.0 |
| MOHGGCOF_12 | 0.0 | 1.0 | 0.0 |
| MOHGGCOF_13 | 0.0 | 1.0 | 0.0 |
| MOHGGCOF_14 | 0.0 | 1.0 | 0.0 |
| MOHGGCOF_15 | 0.0 | 1.0 | 0.0 |
| MOHGGCOF_16 | 0.0 | 1.0 | 0.0 |
| MOHGGCOF_17 | 0.0 | 1.0 | 0.0 |
| MOHGGCOF_18 | 0.0 | 1.0 | 0.0 |
| MOHGGCOF_19 | 0.0 | 1.0 | 0.0 |
| MOHGGCOF_20 | 0.0 | 1.0 | 0.0 |
| MOHGGCOF_21 | 0.0 | 1.0 | 0.0 |
| MOHGGCOF_22 | 0.0 | 1.0 | 0.0 |
| MOHGGCOF_23 | 0.0 | 1.0 | 0.0 |
| MOHGGCOF_24 | 0.0 | 1.0 | 0.0 |
| MOHGGCOF_25 | 0.0 | 1.0 | 0.0 |
| MOHGGCOF_26 | 0.0 | 1.0 | 0.0 |
| MOHGGCOF_27 | 0.0 | 1.0 | 0.0 |
| MOHGGCOF_28 | 0.0 | 1.0 | 0.0 |
| MOHGGCOF_29 | 0.0 | 1.0 | 0.0 |
| MOHGGCOF_30 | 0.0 | 1.0 | 0.0 |
| MOHGGCOF_31 | 0.0 | 1.0 | 0.0 |
| MOHGGCOF_32 | 0.0 | 1.0 | 0.0 |
| MOHGGCOF_33 | 0.0 | 1.0 | 0.0 |
| MOHGGCOF_34 | 0.0 | 1.0 | 0.0 |
| MOHGGCOF_35 | 0.0 | 1.0 | 0.0 |
| MOHGGCOF_36 | 0.0 | 1.0 | 0.0 |
| MOHGGCOF_37 | 0.0 | 1.0 | 0.0 |
| MOHGGCOF_38 | 1.0 | 0.0 | 1.0 |
| MOHGGCOF_39 | 0.0 | 1.0 | 0.0 |
| MOHGGCOF_40 | 0.0 | 1.0 | 0.0 |
| MOHGGCOF_41 | 0.0 | 1.0 | 0.0 |
| MOHGGCOF_42 | 0.0 | 1.0 | 0.0 |
| MOHGGCOF_43 | 0.0 | 1.0 | 0.0 |
| MOHGGCOF_44 | 0.0 | 1.0 | 0.0 |
| MOHGGCOF_45 | 0.0 | 1.0 | 0.0 |
| MOHGGCOF_46 | 1.0 | 0.0 | 1.0 |
| MOHGGCOF_47 | 0.0 | 1.0 | 0.0 |
| MOHGGCOF_48 | 0.0 | 1.0 | 0.0 |
| MOHGGCOF_49 | 0.0 | 1.0 | 0.0 |
| MOHGGCOF_50 | 0.0 | 1.0 | 0.0 |
| MOHGGCOF_51 | 0.0 | 1.0 | 0.0 |
| MOHGGCOF_52 | 0.0 | 1.0 | 0.0 |
| MOHGGCOF_53 | 0.0 | 1.0 | 0.0 |
| MOHGGCOF_54 | 0.0 | 1.0 | 0.0 |
| MOHGGCOF_55 | 0.0 | 1.0 | 0.0 |
| MOHGGCOF_56 | 0.0 | 1.0 | 0.0 |
| MOHGGCOF_57 | 0.0 | 1.0 | 0.0 |
| MOHGGCOF_58 | 0.0 | 1.0 | 0.0 |
| MOHGGCOF_59 | 0.0 | 1.0 | 0.0 |
| MOHGGCOF_60 | 0.0 | 1.0 | 0.0 |
| MOHGGCOF_61 | 0.0 | 1.0 | 0.0 |
| MOHGGCOF_62 | 0.0 | 1.0 | 0.0 |
| MOHGGCOF_63 | 0.0 | 1.0 | 0.0 |
| MOHGGCOF_64 | 0.0 | 1.0 | 0.0 |
| MOHGGCOF_65 | 0.0 | 1.0 | 0.0 |
| MOHGGCOF_66 | 0.0 | 1.0 | 0.0 |
| MOHGGCOF_67 | 0.0 | 1.0 | 0.0 |
| MOHGGCOF_68 | 0.0 | 1.0 | 0.0 |
| MOHGGCOF_69 | 0.0 | 1.0 | 0.0 |
| MOHGGCOF_70 | 0.0 | 1.0 | 0.0 |
| MOHGGCOF_71 | 0.0 | 1.0 | 0.0 |
| MOHGGCOF_72 | 0.0 | 1.0 | 0.0 |
| MOHGGCOF_73 | 0.0 | 1.0 | 0.0 |
| MOHGGCOF_74 | 0.0 | 1.0 | 0.0 |
| MOHGGCOF_75 | 0.0 | 1.0 | 0.0 |
| MOHGGCOF_76 | 0.0 | 1.0 | 0.0 |
| MOHGGCOF_77 | 0.0 | 1.0 | 0.0 |
| MOHGGCOF_78 | 0.0 | 1.0 | 0.0 |
| MOHGGCOF_79 | 0.0 | 1.0 | 0.0 |
| MOHGGCOF_80 | 0.0 | 1.0 | 0.0 |
| MOHGGCOF_81 | 0.0 | 1.0 | 0.0 |
| MOHGGCOF_82 | 0.0 | 1.0 | 0.0 |
| MOHGGCOF_83 | 0.0 | 1.0 | 0.0 |
| MOHGGCOF_84 | 0.0 | 1.0 | 0.0 |
| MOHGGCOF_85 | 0.0 | 1.0 | 0.0 |
| MOHGGCOF_87 | 0.0 | 1.0 | 0.0 |
| MOHGGCOF_88 | 0.0 | 1.0 | 0.0 |
| MOHGGCOF_90 | 0.0 | 1.0 | 0.0 |
| MOHGGCOF_91 | 0.0 | 1.0 | 0.0 |
| MOHGGCOF_92 | 0.0 | 1.0 | 0.0 |
| MOHGGCOF_93 | 0.0 | 1.0 | 0.0 |
| MOHGGCOF_94 | 0.0 | 1.0 | 0.0 |
| MOHGGCOF_95 | 0.0 | 1.0 | 0.0 |
| MOHGGCOF_96 | 1.0 | 0.0 | 1.0 |
| MOHGGCOF_97 | 0.0 | 1.0 | 0.0 |
| MOHGGCOF_99 | 0.0 | 1.0 | 0.0 |
| MOHGGCOF_100 | 0.0 | 1.0 | 0.0 |
| MOHGGCOF_101 | 0.0 | 1.0 | 0.0 |
| MOHGGCOF_102 | 0.0 | 1.0 | 0.0 |
| MOHGGCOF_104 | 0.0 | 1.0 | 0.0 |
| MOHGGCOF_106 | 0.0 | 1.0 | 0.0 |
| MOHGGCOF_107 | 0.0 | 1.0 | 0.0 |
| MOHGGCOF_108 | 0.0 | 1.0 | 0.0 |
| MOHGGCOF_110 | 0.0 | 1.0 | 0.0 |
| MOHGGCOF_111 | 0.0 | 1.0 | 0.0 |
| MOHGGCOF_112 | 0.0 | 1.0 | 0.0 |
| MOHGGCOF_115 | 0.0 | 1.0 | 0.0 |
| MOHGGCOF_116 | 0.0 | 1.0 | 0.0 |
| MOHGGCOF_118 | 0.0 | 1.0 | 0.0 |
| MOHGGCOF_120 | 0.0 | 1.0 | 0.0 |
| MOHGGCOF_121 | 1.0 | 0.0 | 1.0 |
| MOHGGCOF_123 | 0.0 | 1.0 | 0.0 |
| MOHGGCOF_124 | 0.0 | 1.0 | 0.0 |
| MOHGGCOF_125 | 0.0 | 1.0 | 0.0 |
| MOHGGCOF_126 | 0.0 | 1.0 | 0.0 |
| MOHGGCOF_127 | 0.0 | 1.0 | 0.0 |
| MOHGGCOF_128 | 0.0 | 1.0 | 0.0 |
| MOHGGCOF_129 | 1.0 | 0.0 | 1.0 |
| MOHGGCOF_130 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| MOHGGCOF_1 | 114630 | 142072 | False | Myoviridae | VOG0753, VOG4705, VOG7718, VOG4862, VOG4691, VOG4550, VOG3894, VOG4885, VOG4865, VOG6163, VOG3892, VOG3891, VOG3890, VOG0749, VOG4685, VOG4773, VOG10227, VOG0796, VOG5447, VOG4861, VOG6875, VOG0189, VOG3490, VOG9667 |
| MOHGGCOF_20 | 10178 | 42621 | False | Siphoviridae | VOG6513, VOG0275, VOG4602, VOG9667, VOG9667, VOG0650, VOG4552, VOG4603, VOG8233, VOG0186, VOG4566, VOG0189, VOG4693, VOG1985, VOG0198, VOG0713, VOG4841, VOG4581, VOG1886, VOG0202, VOG4556, VOG4573, VOG4553, VOG5361, VOG0204, VOG4589, VOG0539, VOG0704, VOG4586, VOG0541, VOG5910, VOG6163, VOG0801 |
Phigaro v2.4.0 (Database: Jan. 2024 release)