Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 5 | 10 | 9 | 14.35 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| OIDMAAEK_77 | 50231 | 50824 | tmrB | ARO:3003059 | tmrB | 97.97 | 100.00 | AL009126.3 |
| OIDMAAEK_51 | 38413 | 38730 | ykkD | ARO:3003064 | ykkD | 99.05 | 100.00 | AL009126.1 |
| OIDMAAEK_51 | 38075 | 38413 | ykkC | ARO:3003063 | ykkC | 100.00 | 100.00 | AL009126.1 |
| OIDMAAEK_15 | 18735 | 20360 | vmlR | ARO:3004476 | vmlR | 96.49 | 98.90 | NC_000964.3 |
| OIDMAAEK_61 | 250 | 2820 | Bacillus subtilis mprF | ARO:3003324 | Bsub_mprF | 99.77 | 100.00 | AL009126.3 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| No hit found | |||||||
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| OIDMAAEK_15 | 18735 | 20357 | vmlR | ABC-F type ribosomal protection protein VmlR | BLASTX | 96.49 | 98.90 | WP_003234144.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| OIDMAAEK_7 | 69098 | 69883 | dhbA | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.62 | 100.00 | NP_391080 |
| OIDMAAEK_7 | 72770 | 73708 | dhbB | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.57 | 100.00 | NP_391077 |
| OIDMAAEK_47 | 32595 | 33236 | hlyIII | 0.0 | Hemolysin III | Exotoxin | 99.53 | 100.00 | NP_390062 |
| OIDMAAEK_28 | 12980 | 14156 | capB | 0.0 | Capsule | Immune modulation | 99.24 | 99.92 | NP_391471 |
| OIDMAAEK_84 | 9215 | 10978 | dep/capD | 0.0 | Capsule | Immune modulation | 99.15 | 100.00 | NP_389723 |
| OIDMAAEK_28 | 11355 | 12497 | capA | 0.0 | Capsule | Immune modulation | 98.86 | 100.00 | NP_391469 |
| OIDMAAEK_7 | 73728 | 80864 | dhbF | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.85 | 100.00 | NP_391076 |
| OIDMAAEK_7 | 71123 | 72742 | dhbE | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.77 | 100.00 | NP_391078 |
| OIDMAAEK_28 | 12516 | 12965 | capC | 0.0 | Capsule | Immune modulation | 98.67 | 100.00 | NP_391470 |
| OIDMAAEK_7 | 69909 | 71105 | dhbC | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.66 | 100.00 | NP_391079 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| OIDMAAEK_3 | 28163 | 27027 | tufA | 0.0 | elongation factor Tu | 84.70 | 95.69 | AHM69299 |
| OIDMAAEK_4 | 11324 | 10455 | pdxS | 1.12e-151 | encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS | 83.79 | 98.31 | ACE61268 |
| OIDMAAEK_49 | 92530 | 91754 | CodY | 1.82e-125 | nutrient-responsive regulator | 81.08 | 100.00 | ACJ80679 |
| OIDMAAEK_46 | 74196 | 74765 | ClpP | 9.58e-100 | part of proteolytic complex | 81.05 | 97.44 | BAB94595 |
| OIDMAAEK_82 | 23361 | 23753 | spxA1 | 3.32e-71 | redox-responsive transcription factor | 83.97 | 100.00 | AAF21893 |
| OIDMAAEK_82 | 23361 | 23753 | spx | 1.98e-69 | essential gene which is a thiol/oxidative stress sensor | 80.15 | 100.00 | BAB57159 |
| OIDMAAEK_5 | 35015 | 34773 | SpoVG | 1.03e-38 | RNA-binding protein | 81.48 | 79.41 | AAT02994 |
| OIDMAAEK_15 | 54484 | 54287 | CspA | 2.57e-32 | cold shock protein | 90.91 | 100.00 | CAA62903 |
| OIDMAAEK_47 | 44468 | 44662 | CspD | 2.79e-29 | cold shock protein | 81.54 | 98.48 | CAC99957 |
| OIDMAAEK_20 | 200854 | 201048 | CspB | 2.12e-27 | cold shock protein | 83.08 | 98.48 | CAD00094 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| OIDMAAEK_62 | 1296 | 1456 | Col(SD853) | 161 / 194 | 83.85 | NC015392 |
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| OIDMAAEK_1 | 0.0 | 1.0 | 0.0 |
| OIDMAAEK_2 | 0.0 | 1.0 | 0.0 |
| OIDMAAEK_3 | 0.0 | 1.0 | 0.0 |
| OIDMAAEK_4 | 0.0 | 1.0 | 0.0 |
| OIDMAAEK_5 | 0.0 | 1.0 | 0.0 |
| OIDMAAEK_6 | 0.0 | 1.0 | 0.0 |
| OIDMAAEK_7 | 0.0 | 1.0 | 0.0 |
| OIDMAAEK_8 | 1.0 | 0.0 | 1.0 |
| OIDMAAEK_9 | 0.0 | 1.0 | 0.0 |
| OIDMAAEK_11 | 0.0 | 1.0 | 0.0 |
| OIDMAAEK_12 | 0.0 | 1.0 | 0.0 |
| OIDMAAEK_13 | 1.0 | 0.0 | 1.0 |
| OIDMAAEK_14 | 0.0 | 1.0 | 0.0 |
| OIDMAAEK_15 | 0.0 | 1.0 | 0.0 |
| OIDMAAEK_17 | 0.0 | 1.0 | 0.0 |
| OIDMAAEK_18 | 0.0 | 1.0 | 0.0 |
| OIDMAAEK_19 | 0.0 | 1.0 | 0.0 |
| OIDMAAEK_20 | 0.0 | 1.0 | 0.0 |
| OIDMAAEK_21 | 0.0 | 1.0 | 0.0 |
| OIDMAAEK_22 | 0.0 | 1.0 | 0.0 |
| OIDMAAEK_23 | 0.0 | 1.0 | 0.0 |
| OIDMAAEK_24 | 0.0 | 1.0 | 0.0 |
| OIDMAAEK_25 | 0.0 | 1.0 | 0.0 |
| OIDMAAEK_26 | 0.0 | 1.0 | 0.0 |
| OIDMAAEK_28 | 0.0 | 1.0 | 0.0 |
| OIDMAAEK_29 | 0.0 | 1.0 | 0.0 |
| OIDMAAEK_31 | 0.0 | 1.0 | 0.0 |
| OIDMAAEK_32 | 0.0 | 1.0 | 0.0 |
| OIDMAAEK_33 | 0.0 | 1.0 | 0.0 |
| OIDMAAEK_34 | 0.0 | 1.0 | 0.0 |
| OIDMAAEK_35 | 0.0 | 1.0 | 0.0 |
| OIDMAAEK_36 | 0.0 | 1.0 | 0.0 |
| OIDMAAEK_37 | 1.0 | 0.0 | 1.0 |
| OIDMAAEK_38 | 0.0 | 1.0 | 0.0 |
| OIDMAAEK_39 | 0.0 | 1.0 | 0.0 |
| OIDMAAEK_40 | 0.0 | 1.0 | 0.0 |
| OIDMAAEK_41 | 0.0 | 1.0 | 0.0 |
| OIDMAAEK_42 | 0.0 | 1.0 | 0.0 |
| OIDMAAEK_43 | 0.0 | 1.0 | 0.0 |
| OIDMAAEK_44 | 0.0 | 1.0 | 0.0 |
| OIDMAAEK_45 | 0.0 | 1.0 | 0.0 |
| OIDMAAEK_46 | 0.0 | 1.0 | 0.0 |
| OIDMAAEK_47 | 0.0 | 1.0 | 0.0 |
| OIDMAAEK_48 | 1.0 | 0.0 | 1.0 |
| OIDMAAEK_49 | 0.0 | 1.0 | 0.0 |
| OIDMAAEK_50 | 1.0 | 0.0 | 1.0 |
| OIDMAAEK_51 | 0.0 | 1.0 | 0.0 |
| OIDMAAEK_52 | 0.0 | 1.0 | 0.0 |
| OIDMAAEK_53 | 0.0 | 1.0 | 0.0 |
| OIDMAAEK_54 | 0.0 | 1.0 | 0.0 |
| OIDMAAEK_55 | 0.0 | 1.0 | 0.0 |
| OIDMAAEK_57 | 0.0 | 1.0 | 0.0 |
| OIDMAAEK_58 | 0.0 | 1.0 | 0.0 |
| OIDMAAEK_59 | 0.0 | 1.0 | 0.0 |
| OIDMAAEK_60 | 0.0 | 1.0 | 0.0 |
| OIDMAAEK_61 | 0.0 | 1.0 | 0.0 |
| OIDMAAEK_62 | 1.0 | 0.0 | 1.0 |
| OIDMAAEK_63 | 0.0 | 1.0 | 0.0 |
| OIDMAAEK_64 | 0.0 | 1.0 | 0.0 |
| OIDMAAEK_65 | 0.0 | 1.0 | 0.0 |
| OIDMAAEK_66 | 0.0 | 1.0 | 0.0 |
| OIDMAAEK_67 | 0.0 | 1.0 | 0.0 |
| OIDMAAEK_68 | 0.0 | 1.0 | 0.0 |
| OIDMAAEK_69 | 0.0 | 1.0 | 0.0 |
| OIDMAAEK_70 | 0.0 | 1.0 | 0.0 |
| OIDMAAEK_71 | 0.0 | 1.0 | 0.0 |
| OIDMAAEK_72 | 0.0 | 1.0 | 0.0 |
| OIDMAAEK_73 | 0.0 | 1.0 | 0.0 |
| OIDMAAEK_74 | 0.0 | 1.0 | 0.0 |
| OIDMAAEK_75 | 0.0 | 1.0 | 0.0 |
| OIDMAAEK_76 | 0.0 | 1.0 | 0.0 |
| OIDMAAEK_77 | 0.0 | 1.0 | 0.0 |
| OIDMAAEK_78 | 1.0 | 0.0 | 1.0 |
| OIDMAAEK_79 | 0.0 | 1.0 | 0.0 |
| OIDMAAEK_80 | 0.0 | 1.0 | 0.0 |
| OIDMAAEK_81 | 0.0 | 1.0 | 0.0 |
| OIDMAAEK_82 | 0.0 | 1.0 | 0.0 |
| OIDMAAEK_83 | 0.0 | 1.0 | 0.0 |
| OIDMAAEK_84 | 0.0 | 1.0 | 0.0 |
| OIDMAAEK_85 | 0.0 | 1.0 | 0.0 |
| OIDMAAEK_86 | 0.0 | 1.0 | 0.0 |
| OIDMAAEK_87 | 1.0 | 0.0 | 1.0 |
| OIDMAAEK_88 | 0.0 | 1.0 | 0.0 |
| OIDMAAEK_89 | 0.0 | 1.0 | 0.0 |
| OIDMAAEK_90 | 0.0 | 1.0 | 0.0 |
| OIDMAAEK_91 | 0.0 | 1.0 | 0.0 |
| OIDMAAEK_92 | 1.0 | 0.0 | 1.0 |
| OIDMAAEK_93 | 0.0 | 1.0 | 0.0 |
| OIDMAAEK_94 | 0.0 | 1.0 | 0.0 |
| OIDMAAEK_97 | 0.0 | 1.0 | 0.0 |
| OIDMAAEK_98 | 0.0 | 1.0 | 0.0 |
| OIDMAAEK_99 | 0.0 | 1.0 | 0.0 |
| OIDMAAEK_100 | 0.0 | 1.0 | 0.0 |
| OIDMAAEK_101 | 0.0 | 1.0 | 0.0 |
| OIDMAAEK_102 | 0.0 | 1.0 | 0.0 |
| OIDMAAEK_103 | 1.0 | 0.0 | 1.0 |
| OIDMAAEK_104 | 0.0 | 1.0 | 0.0 |
| OIDMAAEK_105 | 0.0 | 1.0 | 0.0 |
| OIDMAAEK_106 | 0.0 | 1.0 | 0.0 |
| OIDMAAEK_107 | 1.0 | 0.0 | 1.0 |
| OIDMAAEK_109 | 1.0 | 0.0 | 1.0 |
| OIDMAAEK_110 | 0.0 | 1.0 | 0.0 |
| OIDMAAEK_111 | 0.0 | 1.0 | 0.0 |
| OIDMAAEK_112 | 0.0 | 1.0 | 0.0 |
| OIDMAAEK_113 | 0.0 | 1.0 | 0.0 |
| OIDMAAEK_114 | 0.0 | 1.0 | 0.0 |
| OIDMAAEK_115 | 1.0 | 0.0 | 1.0 |
| OIDMAAEK_116 | 1.0 | 0.0 | 1.0 |
| OIDMAAEK_117 | 0.0 | 1.0 | 0.0 |
| OIDMAAEK_118 | 0.0 | 1.0 | 0.0 |
| OIDMAAEK_119 | 0.0 | 1.0 | 0.0 |
| OIDMAAEK_122 | 0.0 | 1.0 | 0.0 |
| OIDMAAEK_123 | 1.0 | 0.0 | 1.0 |
| OIDMAAEK_125 | 0.0 | 1.0 | 0.0 |
| OIDMAAEK_126 | 0.0 | 1.0 | 0.0 |
| OIDMAAEK_127 | 1.0 | 0.0 | 1.0 |
| OIDMAAEK_128 | 0.0 | 1.0 | 0.0 |
| OIDMAAEK_131 | 0.0 | 1.0 | 0.0 |
| OIDMAAEK_132 | 0.0 | 1.0 | 0.0 |
| OIDMAAEK_138 | 1.0 | 0.0 | 1.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| OIDMAAEK_22 | 61592 | 82033 | False | Siphoviridae | VOG4574, VOG0136, VOG0753, VOG5615, VOG5535, VOG3390, VOG4599, VOG4605, VOG9745, VOG0701, VOG10044, VOG0698, VOG0697, VOG0696, VOG0695, VOG2621, VOG4572, VOG9317, VOG0692, VOG0691, VOG10227, VOG0796, VOG7983 |
| OIDMAAEK_22 | 88561 | 95431 | False | Siphoviridae | VOG4600, VOG4552, VOG0650, VOG9667, VOG9667, VOG4662, VOG4602 |
| OIDMAAEK_40 | 27791 | 35994 | False | Unknown | VOG9667, VOG3490, VOG0189, VOG4861, VOG5447, VOG0796, VOG10227, VOG4773 |
| OIDMAAEK_51 | 2931 | 4808 | False | Myoviridae | VOG4691, VOG4862 |
| OIDMAAEK_66 | 30934 | 34683 | False | Siphoviridae | VOG0181, VOG0737, VOG4556, VOG0787, VOG1638, VOG4552 |
| OIDMAAEK_70 | 4203 | 16611 | False | Siphoviridae | VOG5910, VOG0541, VOG4586, VOG0704, VOG0539, VOG4589, VOG0204, VOG5361, VOG4553, VOG4573, VOG4556, VOG0202, VOG1886, VOG4581, VOG4841, VOG0713, VOG7884, VOG0275 |
Phigaro v2.4.0 (Database: Jan. 2024 release)