Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 11 | 10 | 9 | 17.38 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| IBGAFHOF_6 | 369502 | 370353 | aadK | ARO:3002627 | aadK | 98.24 | 99.65 | AL009126.1 |
| IBGAFHOF_21 | 62273 | 62590 | ykkD | ARO:3003064 | ykkD | 98.10 | 100.00 | AL009126.1 |
| IBGAFHOF_21 | 61935 | 62273 | ykkC | ARO:3003063 | ykkC | 98.21 | 100.00 | AL009126.1 |
| IBGAFHOF_30 | 53727 | 56297 | Bacillus subtilis mprF | ARO:3003324 | Bsub_mprF | 99.77 | 100.00 | AL009126.3 |
| IBGAFHOF_23 | 45209 | 46855 | vmlR | ARO:3004476 | vmlR | 98.18 | 100.18 | NC_000964.3 |
| IBGAFHOF_1 | 41040 | 41960 | mphK | ARO:3004541 | mphK | 98.04 | 100.00 | CP010314.1 |
| IBGAFHOF_1 | 27708 | 29147 | lmrB | ARO:3002813 | lmrB | 99.58 | 100.42 | JYFL01000006.1 |
| IBGAFHOF_8 | 19040 | 19633 | tmrB | ARO:3003059 | tmrB | 99.49 | 100.00 | AL009126.3 |
| IBGAFHOF_48 | 742 | 1944 | blt | ARO:3003006 | blt | 98.00 | 100.00 | L32599.1 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| IBGAFHOF_6 | 369502 | 370353 | aadK | Streptomycin | 98.48 | 99.65 | M26879 |
| IBGAFHOF_1 | 41040 | 41960 | mph(K) | Spiramycin, Telithromycin | 97.50 | 100.00 | NC_000964 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| IBGAFHOF_6 | 369502 | 370350 | aadK | aminoglycoside 6-adenylyltransferase AadK | BLASTX | 98.24 | 100.00 | WP_003229862.1 |
| IBGAFHOF_23 | 45212 | 46855 | vmlR | ABC-F type ribosomal protection protein VmlR | BLASTX | 98.18 | 100.00 | WP_003234144.1 |
| IBGAFHOF_1 | 41043 | 41960 | mphK | macrolide 2'-phosphotransferase MphK | BLASTX | 98.04 | 100.00 | WP_003246254.1 |
| IBGAFHOF_31 | 72340 | 72858 | satA | streptothricin N-acetyltransferase SatA | BLASTX | 93.06 | 100.00 | WP_003242546.1 |
| IBGAFHOF_17 | 2766 | 5363 | rphC | rifamycin-inactivating phosphotransferase RphC | BLASTX | 80.48 | 100.00 | WP_087347987.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| IBGAFHOF_10 | 254213 | 255394 | capB | 0.0 | Capsule | Immune modulation | 99.66 | 100.00 | NP_391471 |
| IBGAFHOF_7 | 171887 | 172672 | dhbA | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.49 | 100.00 | NP_391080 |
| IBGAFHOF_10 | 252588 | 253730 | capA | 0.0 | Capsule | Immune modulation | 99.30 | 100.00 | NP_391469 |
| IBGAFHOF_7 | 176528 | 183664 | dhbF | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.16 | 100.00 | NP_391076 |
| IBGAFHOF_7 | 175570 | 176508 | dhbB | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.15 | 100.00 | NP_391077 |
| IBGAFHOF_7 | 172698 | 173894 | dhbC | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.00 | 100.00 | NP_391079 |
| IBGAFHOF_17 | 56436 | 58199 | dep/capD | 0.0 | Capsule | Immune modulation | 98.98 | 100.00 | NP_389723 |
| IBGAFHOF_15 | 91505 | 92146 | hlyIII | 0.0 | Hemolysin III | Exotoxin | 98.91 | 100.00 | NP_390062 |
| IBGAFHOF_10 | 253749 | 254198 | capC | 0.0 | Capsule | Immune modulation | 98.89 | 100.00 | NP_391470 |
| IBGAFHOF_7 | 173923 | 175542 | dhbE | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 96.73 | 100.00 | NP_391078 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| IBGAFHOF_39 | 31714 | 32850 | tufA | 0.0 | elongation factor Tu | 84.96 | 95.69 | AHM69299 |
| IBGAFHOF_19 | 11360 | 10491 | pdxS | 1.12e-151 | encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS | 83.79 | 98.31 | ACE61268 |
| IBGAFHOF_3 | 220245 | 221021 | CodY | 2.57e-125 | nutrient-responsive regulator | 81.08 | 100.00 | ACJ80679 |
| IBGAFHOF_10 | 104226 | 104795 | ClpP | 2.27e-99 | part of proteolytic complex | 81.05 | 97.44 | BAB94595 |
| IBGAFHOF_5 | 41793 | 41401 | spxA1 | 1.83e-70 | redox-responsive transcription factor | 83.97 | 100.00 | AAF21893 |
| IBGAFHOF_5 | 41793 | 41401 | spx | 1.09e-68 | essential gene which is a thiol/oxidative stress sensor | 80.15 | 100.00 | BAB57159 |
| IBGAFHOF_43 | 11052 | 11294 | SpoVG | 6.26e-39 | RNA-binding protein | 81.48 | 79.41 | AAT02994 |
| IBGAFHOF_23 | 5161 | 5358 | CspA | 3.26e-32 | cold shock protein | 90.91 | 100.00 | CAA62903 |
| IBGAFHOF_15 | 103376 | 103570 | CspD | 1.16e-28 | cold shock protein | 81.54 | 98.48 | CAC99957 |
| IBGAFHOF_28 | 32113 | 31919 | CspB | 3.48e-28 | cold shock protein | 83.08 | 98.48 | CAD00094 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| IBGAFHOF_1 | 0.0 | 1.0 | 0.0 |
| IBGAFHOF_2 | 0.0 | 1.0 | 0.0 |
| IBGAFHOF_3 | 0.0 | 1.0 | 0.0 |
| IBGAFHOF_4 | 0.0 | 1.0 | 0.0 |
| IBGAFHOF_5 | 0.0 | 1.0 | 0.0 |
| IBGAFHOF_6 | 0.0 | 1.0 | 0.0 |
| IBGAFHOF_7 | 0.0 | 1.0 | 0.0 |
| IBGAFHOF_8 | 0.0 | 1.0 | 0.0 |
| IBGAFHOF_9 | 0.0 | 1.0 | 0.0 |
| IBGAFHOF_10 | 0.0 | 1.0 | 0.0 |
| IBGAFHOF_11 | 0.0 | 1.0 | 0.0 |
| IBGAFHOF_12 | 0.0 | 1.0 | 0.0 |
| IBGAFHOF_13 | 0.0 | 1.0 | 0.0 |
| IBGAFHOF_14 | 0.0 | 1.0 | 0.0 |
| IBGAFHOF_15 | 0.0 | 1.0 | 0.0 |
| IBGAFHOF_16 | 0.0 | 1.0 | 0.0 |
| IBGAFHOF_17 | 0.0 | 1.0 | 0.0 |
| IBGAFHOF_18 | 0.0 | 1.0 | 0.0 |
| IBGAFHOF_19 | 0.0 | 1.0 | 0.0 |
| IBGAFHOF_21 | 0.0 | 1.0 | 0.0 |
| IBGAFHOF_22 | 0.0 | 1.0 | 0.0 |
| IBGAFHOF_23 | 0.0 | 1.0 | 0.0 |
| IBGAFHOF_24 | 0.0 | 1.0 | 0.0 |
| IBGAFHOF_25 | 0.0 | 1.0 | 0.0 |
| IBGAFHOF_26 | 0.0 | 1.0 | 0.0 |
| IBGAFHOF_27 | 0.0 | 1.0 | 0.0 |
| IBGAFHOF_28 | 0.0 | 1.0 | 0.0 |
| IBGAFHOF_29 | 1.0 | 0.0 | 1.0 |
| IBGAFHOF_30 | 0.0 | 1.0 | 0.0 |
| IBGAFHOF_31 | 0.0 | 1.0 | 0.0 |
| IBGAFHOF_32 | 0.0 | 1.0 | 0.0 |
| IBGAFHOF_33 | 0.0 | 1.0 | 0.0 |
| IBGAFHOF_34 | 0.0 | 1.0 | 0.0 |
| IBGAFHOF_35 | 0.0 | 1.0 | 0.0 |
| IBGAFHOF_36 | 0.0 | 1.0 | 0.0 |
| IBGAFHOF_37 | 0.0 | 1.0 | 0.0 |
| IBGAFHOF_38 | 0.0 | 1.0 | 0.0 |
| IBGAFHOF_39 | 0.0 | 1.0 | 0.0 |
| IBGAFHOF_40 | 1.0 | 0.0 | 1.0 |
| IBGAFHOF_42 | 0.0 | 1.0 | 0.0 |
| IBGAFHOF_43 | 0.0 | 1.0 | 0.0 |
| IBGAFHOF_44 | 0.0 | 1.0 | 0.0 |
| IBGAFHOF_45 | 0.0 | 1.0 | 0.0 |
| IBGAFHOF_46 | 0.0 | 1.0 | 0.0 |
| IBGAFHOF_47 | 0.0 | 1.0 | 0.0 |
| IBGAFHOF_48 | 0.0 | 1.0 | 0.0 |
| IBGAFHOF_49 | 0.0 | 1.0 | 0.0 |
| IBGAFHOF_50 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| IBGAFHOF_10 | 8195 | 18725 | True | Siphoviridae | VOG6587, VOG0189, VOG4566, VOG7581, VOG9667, VOG9667, VOG1563, VOG0275, VOG0275 |
| IBGAFHOF_21 | 6308 | 32471 | False | Myoviridae | VOG3490, VOG0189, VOG4861, VOG5447, VOG0796, VOG10227, VOG4773, VOG4685, VOG0749, VOG3890, VOG3891, VOG3892, VOG6163, VOG4865, VOG4885, VOG3894, VOG4550, VOG4691, VOG4862, VOG7718, VOG4705 |
| IBGAFHOF_24 | 1 | 27513 | False | Siphoviridae | VOG4612, VOG4626, VOG4619, VOG0801, VOG6163, VOG5910, VOG0541, VOG4586, VOG0704, VOG0539, VOG4589, VOG0204, VOG5361, VOG4553, VOG4568, VOG4556, VOG3644, VOG4544, VOG4581, VOG7403, VOG4841, VOG0198, VOG7372, VOG2752, VOG4606, VOG1227, VOG1936, VOG0535, VOG0650 |
Phigaro v2.4.0 (Database: Jan. 2024 release)