Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 10 | 10 | 9 | 16.76 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| FFKAFJCE_31 | 84875 | 85213 | ykkC | ARO:3003063 | ykkC | 98.21 | 100.00 | AL009126.1 |
| FFKAFJCE_31 | 84558 | 84875 | ykkD | ARO:3003064 | ykkD | 98.10 | 100.00 | AL009126.1 |
| FFKAFJCE_47 | 47405 | 47998 | tmrB | ARO:3003059 | tmrB | 99.49 | 100.00 | AL009126.3 |
| FFKAFJCE_21 | 41880 | 44450 | Bacillus subtilis mprF | ARO:3003324 | Bsub_mprF | 99.77 | 100.00 | AL009126.3 |
| FFKAFJCE_46 | 41019 | 41783 | mphK | ARO:3004541 | mphK | 97.61 | 83.01 | CP010314.1 |
| FFKAFJCE_46 | 27691 | 29124 | lmrB | ARO:3002813 | lmrB | 98.95 | 100.00 | JYFL01000006.1 |
| FFKAFJCE_5 | 3649 | 4503 | aadK | ARO:3002627 | aadK | 98.94 | 100.00 | AL009126.1 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| FFKAFJCE_5 | 3649 | 4503 | aadK | Streptomycin | 98.83 | 100.00 | M26879 |
| FFKAFJCE_46 | 41019 | 41777 | mph(K) | Spiramycin, Telithromycin | 97.63 | 82.41 | NC_000964 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| FFKAFJCE_5 | 3652 | 4503 | aadK | aminoglycoside 6-adenylyltransferase AadK | BLASTX | 98.94 | 100.00 | WP_003229862.1 |
| FFKAFJCE_46 | 41031 | 41777 | mphK | macrolide 2'-phosphotransferase MphK | PARTIALX | 98.39 | 81.37 | WP_003246254.1 |
| FFKAFJCE_82 | 5682 | 7289 | vmlR | ABC-F type ribosomal protection protein VmlR | INTERNAL_STOP | 95.61 | 100.00 | WP_003234144.1 |
| FFKAFJCE_8 | 37026 | 37544 | satA | streptothricin N-acetyltransferase SatA | BLASTX | 91.91 | 100.00 | WP_003242546.1 |
| FFKAFJCE_63 | 73615 | 76212 | rphC | rifamycin-inactivating phosphotransferase RphC | BLASTX | 80.48 | 100.00 | WP_087347987.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| FFKAFJCE_40 | 2071 | 3252 | capB | 0.0 | Capsule | Immune modulation | 99.49 | 100.00 | NP_391471 |
| FFKAFJCE_6 | 6433 | 7218 | dhbA | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.49 | 100.00 | NP_391080 |
| FFKAFJCE_40 | 446 | 1588 | capA | 0.0 | Capsule | Immune modulation | 99.30 | 100.00 | NP_391469 |
| FFKAFJCE_6 | 10116 | 11054 | dhbB | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.15 | 100.00 | NP_391077 |
| FFKAFJCE_6 | 7244 | 8440 | dhbC | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.08 | 100.00 | NP_391079 |
| FFKAFJCE_43 | 96926 | 97567 | hlyIII | 0.0 | Hemolysin III | Exotoxin | 98.91 | 100.00 | NP_390062 |
| FFKAFJCE_40 | 1607 | 2056 | capC | 0.0 | Capsule | Immune modulation | 98.89 | 100.00 | NP_391470 |
| FFKAFJCE_6 | 11074 | 18210 | dhbF | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.66 | 100.00 | NP_391076 |
| FFKAFJCE_63 | 20813 | 22565 | dep/capD | 0.0 | Capsule | Immune modulation | 98.30 | 99.94 | NP_389723 |
| FFKAFJCE_6 | 8469 | 10088 | dhbE | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 97.04 | 100.00 | NP_391078 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| FFKAFJCE_30 | 28137 | 27001 | tufA | 0.0 | elongation factor Tu | 85.75 | 95.69 | AHM69299 |
| FFKAFJCE_65 | 4721 | 5590 | pdxS | 2.03e-151 | encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS | 83.45 | 98.31 | ACE61268 |
| FFKAFJCE_37 | 96880 | 96104 | CodY | 1.86e-125 | nutrient-responsive regulator | 81.08 | 100.00 | ACJ80679 |
| FFKAFJCE_4 | 320 | 889 | ClpP | 1.35e-100 | part of proteolytic complex | 81.05 | 97.44 | BAB94595 |
| FFKAFJCE_19 | 48158 | 47766 | spxA1 | 4.55e-71 | redox-responsive transcription factor | 83.97 | 100.00 | AAF21893 |
| FFKAFJCE_19 | 48158 | 47766 | spx | 2.72e-69 | essential gene which is a thiol/oxidative stress sensor | 80.15 | 100.00 | BAB57159 |
| FFKAFJCE_3 | 20676 | 20918 | SpoVG | 7.50e-39 | RNA-binding protein | 81.48 | 79.41 | AAT02994 |
| FFKAFJCE_7 | 17590 | 17787 | CspA | 2.20e-32 | cold shock protein | 90.91 | 100.00 | CAA62903 |
| FFKAFJCE_43 | 85696 | 85502 | CspD | 6.94e-29 | cold shock protein | 81.54 | 98.48 | CAC99957 |
| FFKAFJCE_56 | 25172 | 24978 | CspB | 4.61e-28 | cold shock protein | 83.08 | 98.48 | CAD00094 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| FFKAFJCE_1 | 0.0 | 1.0 | 0.0 |
| FFKAFJCE_2 | 0.0 | 1.0 | 0.0 |
| FFKAFJCE_3 | 0.0 | 1.0 | 0.0 |
| FFKAFJCE_4 | 0.0 | 1.0 | 0.0 |
| FFKAFJCE_5 | 0.0 | 1.0 | 0.0 |
| FFKAFJCE_6 | 0.0 | 1.0 | 0.0 |
| FFKAFJCE_7 | 0.0 | 1.0 | 0.0 |
| FFKAFJCE_8 | 0.0 | 1.0 | 0.0 |
| FFKAFJCE_9 | 0.0 | 1.0 | 0.0 |
| FFKAFJCE_10 | 0.0 | 1.0 | 0.0 |
| FFKAFJCE_11 | 0.0 | 1.0 | 0.0 |
| FFKAFJCE_12 | 1.0 | 0.0 | 1.0 |
| FFKAFJCE_13 | 1.0 | 0.0 | 1.0 |
| FFKAFJCE_14 | 0.0 | 1.0 | 0.0 |
| FFKAFJCE_15 | 0.0 | 1.0 | 0.0 |
| FFKAFJCE_16 | 0.0 | 1.0 | 0.0 |
| FFKAFJCE_17 | 0.0 | 1.0 | 0.0 |
| FFKAFJCE_18 | 1.0 | 0.0 | 1.0 |
| FFKAFJCE_19 | 0.0 | 1.0 | 0.0 |
| FFKAFJCE_20 | 0.0 | 1.0 | 0.0 |
| FFKAFJCE_21 | 0.0 | 1.0 | 0.0 |
| FFKAFJCE_22 | 0.0 | 1.0 | 0.0 |
| FFKAFJCE_23 | 1.0 | 0.0 | 1.0 |
| FFKAFJCE_24 | 1.0 | 0.0 | 1.0 |
| FFKAFJCE_25 | 0.0 | 1.0 | 0.0 |
| FFKAFJCE_26 | 0.0 | 1.0 | 0.0 |
| FFKAFJCE_28 | 0.0 | 1.0 | 0.0 |
| FFKAFJCE_29 | 0.0 | 1.0 | 0.0 |
| FFKAFJCE_30 | 0.0 | 1.0 | 0.0 |
| FFKAFJCE_31 | 0.0 | 1.0 | 0.0 |
| FFKAFJCE_32 | 0.0 | 1.0 | 0.0 |
| FFKAFJCE_33 | 0.0 | 1.0 | 0.0 |
| FFKAFJCE_34 | 0.0 | 1.0 | 0.0 |
| FFKAFJCE_35 | 0.0 | 1.0 | 0.0 |
| FFKAFJCE_36 | 1.0 | 0.0 | 1.0 |
| FFKAFJCE_37 | 0.0 | 1.0 | 0.0 |
| FFKAFJCE_38 | 0.0 | 1.0 | 0.0 |
| FFKAFJCE_39 | 0.0 | 1.0 | 0.0 |
| FFKAFJCE_40 | 0.0 | 1.0 | 0.0 |
| FFKAFJCE_41 | 0.0 | 1.0 | 0.0 |
| FFKAFJCE_42 | 0.0 | 1.0 | 0.0 |
| FFKAFJCE_43 | 0.0 | 1.0 | 0.0 |
| FFKAFJCE_44 | 1.0 | 0.0 | 1.0 |
| FFKAFJCE_45 | 0.0 | 1.0 | 0.0 |
| FFKAFJCE_46 | 0.0 | 1.0 | 0.0 |
| FFKAFJCE_47 | 0.0 | 1.0 | 0.0 |
| FFKAFJCE_48 | 0.0 | 1.0 | 0.0 |
| FFKAFJCE_49 | 0.0 | 1.0 | 0.0 |
| FFKAFJCE_50 | 0.0 | 1.0 | 0.0 |
| FFKAFJCE_51 | 0.0 | 1.0 | 0.0 |
| FFKAFJCE_52 | 0.0 | 1.0 | 0.0 |
| FFKAFJCE_53 | 0.0 | 1.0 | 0.0 |
| FFKAFJCE_54 | 0.0 | 1.0 | 0.0 |
| FFKAFJCE_55 | 1.0 | 0.0 | 1.0 |
| FFKAFJCE_56 | 0.0 | 1.0 | 0.0 |
| FFKAFJCE_57 | 0.0 | 1.0 | 0.0 |
| FFKAFJCE_58 | 0.0 | 1.0 | 0.0 |
| FFKAFJCE_59 | 0.0 | 1.0 | 0.0 |
| FFKAFJCE_60 | 0.0 | 1.0 | 0.0 |
| FFKAFJCE_61 | 0.0 | 1.0 | 0.0 |
| FFKAFJCE_62 | 0.0 | 1.0 | 0.0 |
| FFKAFJCE_63 | 0.0 | 1.0 | 0.0 |
| FFKAFJCE_64 | 0.0 | 1.0 | 0.0 |
| FFKAFJCE_65 | 0.0 | 1.0 | 0.0 |
| FFKAFJCE_66 | 0.0 | 1.0 | 0.0 |
| FFKAFJCE_67 | 1.0 | 0.0 | 1.0 |
| FFKAFJCE_68 | 0.0 | 1.0 | 0.0 |
| FFKAFJCE_69 | 0.0 | 1.0 | 0.0 |
| FFKAFJCE_70 | 0.0 | 1.0 | 0.0 |
| FFKAFJCE_71 | 0.0 | 1.0 | 0.0 |
| FFKAFJCE_72 | 0.0 | 1.0 | 0.0 |
| FFKAFJCE_73 | 0.0 | 1.0 | 0.0 |
| FFKAFJCE_74 | 0.0 | 1.0 | 0.0 |
| FFKAFJCE_75 | 1.0 | 0.0 | 1.0 |
| FFKAFJCE_77 | 0.0 | 1.0 | 0.0 |
| FFKAFJCE_79 | 0.0 | 1.0 | 0.0 |
| FFKAFJCE_80 | 0.0 | 1.0 | 0.0 |
| FFKAFJCE_81 | 1.0 | 0.0 | 1.0 |
| FFKAFJCE_82 | 0.0 | 1.0 | 0.0 |
| FFKAFJCE_83 | 0.0 | 1.0 | 0.0 |
| FFKAFJCE_84 | 0.0 | 1.0 | 0.0 |
| FFKAFJCE_85 | 0.0 | 1.0 | 0.0 |
| FFKAFJCE_86 | 0.0 | 1.0 | 0.0 |
| FFKAFJCE_87 | 0.0 | 1.0 | 0.0 |
| FFKAFJCE_88 | 1.0 | 0.0 | 1.0 |
| FFKAFJCE_89 | 0.0 | 1.0 | 0.0 |
| FFKAFJCE_90 | 1.0 | 0.0 | 1.0 |
| FFKAFJCE_92 | 0.0 | 1.0 | 0.0 |
| FFKAFJCE_93 | 0.0 | 1.0 | 0.0 |
| FFKAFJCE_94 | 0.0 | 1.0 | 0.0 |
| FFKAFJCE_95 | 0.0 | 1.0 | 0.0 |
| FFKAFJCE_96 | 0.0 | 1.0 | 0.0 |
| FFKAFJCE_97 | 0.0 | 1.0 | 0.0 |
| FFKAFJCE_98 | 0.0 | 1.0 | 0.0 |
| FFKAFJCE_99 | 1.0 | 0.0 | 1.0 |
| FFKAFJCE_100 | 0.0 | 1.0 | 0.0 |
| FFKAFJCE_101 | 0.0 | 1.0 | 0.0 |
| FFKAFJCE_102 | 0.0 | 1.0 | 0.0 |
| FFKAFJCE_103 | 0.0 | 1.0 | 0.0 |
| FFKAFJCE_104 | 1.0 | 0.0 | 1.0 |
| FFKAFJCE_105 | 0.0 | 1.0 | 0.0 |
| FFKAFJCE_106 | 0.0 | 1.0 | 0.0 |
| FFKAFJCE_111 | 0.0 | 1.0 | 0.0 |
| FFKAFJCE_112 | 1.0 | 0.0 | 1.0 |
| FFKAFJCE_113 | 0.0 | 1.0 | 0.0 |
| FFKAFJCE_114 | 0.0 | 1.0 | 0.0 |
| FFKAFJCE_117 | 1.0 | 0.0 | 1.0 |
| FFKAFJCE_118 | 1.0 | 0.0 | 1.0 |
| FFKAFJCE_119 | 0.0 | 1.0 | 0.0 |
| FFKAFJCE_121 | 0.0 | 1.0 | 0.0 |
| FFKAFJCE_122 | 0.0 | 1.0 | 0.0 |
| FFKAFJCE_123 | 0.0 | 1.0 | 0.0 |
| FFKAFJCE_124 | 0.0 | 1.0 | 0.0 |
| FFKAFJCE_126 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| FFKAFJCE_13 | 19839 | 39366 | False | Siphoviridae | VOG4571, VOG4730, VOG1838, VOG3212, VOG0371, VOG1093, VOG5543, VOG0422, VOG0026, VOG7980, VOG5324, VOG10937, VOG7255, VOG4134, VOG1563, VOG2215, VOG4551, VOG0025, VOG0044, VOG5446, VOG0189, VOG6168 |
| FFKAFJCE_25 | 86349 | 96879 | True | Siphoviridae | VOG3490, VOG0275, VOG0275, VOG1563, VOG9667, VOG9667, VOG7581, VOG4566, VOG0189, VOG6587 |
| FFKAFJCE_31 | 114677 | 142038 | False | Myoviridae | VOG0753, VOG4705, VOG7718, VOG4862, VOG4691, VOG4550, VOG3894, VOG4885, VOG4865, VOG6163, VOG3892, VOG3891, VOG3890, VOG0749, VOG4685, VOG4773, VOG10227, VOG0796, VOG5447, VOG4861, VOG6875, VOG0189, VOG3490, VOG9667 |
| FFKAFJCE_54 | 23702 | 48882 | False | Siphoviridae | VOG0189, VOG0536, VOG1336, VOG5836, VOG4567, VOG3444, VOG0397, VOG0044, VOG0198, VOG0275, VOG7884, VOG0713, VOG4841, VOG4581, VOG1886, VOG0202, VOG4556, VOG4573, VOG4553, VOG5361, VOG0204, VOG4589, VOG0539, VOG0704, VOG4586, VOG0541, VOG5910, VOG6163, VOG0801 |
Phigaro v2.4.0 (Database: Jan. 2024 release)