Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 12 | 10 | 9 | 18.03 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| KFEIOIPB_30 | 85316 | 87886 | Bacillus subtilis mprF | ARO:3003324 | Bsub_mprF | 99.77 | 100.00 | AL009126.3 |
| KFEIOIPB_49 | 84828 | 85166 | ykkC | ARO:3003063 | ykkC | 98.21 | 100.00 | AL009126.1 |
| KFEIOIPB_49 | 84511 | 84828 | ykkD | ARO:3003064 | ykkD | 98.10 | 100.00 | AL009126.1 |
| KFEIOIPB_4 | 41818 | 42411 | tmrB | ARO:3003059 | tmrB | 100.00 | 100.00 | AL009126.3 |
| KFEIOIPB_72 | 27549 | 28400 | aadK | ARO:3002627 | aadK | 98.24 | 99.65 | AL009126.1 |
| KFEIOIPB_78 | 20745 | 21914 | bmr | ARO:3003007 | bmr | 99.49 | 100.00 | M33768.1 |
| KFEIOIPB_59 | 6846 | 8048 | blt | ARO:3003006 | blt | 99.25 | 100.00 | L32599.1 |
| KFEIOIPB_18 | 6185 | 7105 | mphK | ARO:3004541 | mphK | 98.68 | 100.00 | CP010314.1 |
| KFEIOIPB_53 | 5903 | 7336 | lmrB | ARO:3002813 | lmrB | 99.16 | 100.00 | JYFL01000006.1 |
| KFEIOIPB_56 | 5850 | 7493 | vmlR | ARO:3004476 | vmlR | 98.32 | 100.00 | NC_000964.3 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| KFEIOIPB_72 | 27549 | 28400 | aadK | Streptomycin | 98.48 | 99.65 | M26879 |
| KFEIOIPB_18 | 6185 | 7105 | mph(K) | Spiramycin, Telithromycin | 98.05 | 100.00 | NC_000964 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| KFEIOIPB_18 | 6197 | 7105 | mphK | macrolide 2'-phosphotransferase MphK | BLASTX | 98.68 | 99.02 | WP_003246254.1 |
| KFEIOIPB_72 | 27549 | 28397 | aadK | aminoglycoside 6-adenylyltransferase AadK | BLASTX | 98.24 | 100.00 | WP_003229862.1 |
| KFEIOIPB_56 | 5853 | 7493 | vmlR | ABC-F type ribosomal protection protein VmlR | BLASTX | 96.71 | 99.82 | WP_003234144.1 |
| KFEIOIPB_22 | 61915 | 62307 | satA | streptothricin N-acetyltransferase SatA | PARTIALX | 91.60 | 75.72 | WP_003242546.1 |
| KFEIOIPB_25 | 5443 | 8040 | rphC | rifamycin-inactivating phosphotransferase RphC | BLASTX | 80.48 | 100.00 | WP_087347987.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| KFEIOIPB_127 | 1857 | 3038 | capB | 0.0 | Capsule | Immune modulation | 99.66 | 100.00 | NP_391471 |
| KFEIOIPB_5 | 16065 | 16850 | dhbA | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.36 | 100.00 | NP_391080 |
| KFEIOIPB_127 | 232 | 1374 | capA | 0.0 | Capsule | Immune modulation | 99.30 | 100.00 | NP_391469 |
| KFEIOIPB_25 | 59127 | 60890 | dep/capD | 0.0 | Capsule | Immune modulation | 98.98 | 100.00 | NP_389723 |
| KFEIOIPB_5 | 19737 | 20675 | dhbB | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.94 | 100.00 | NP_391077 |
| KFEIOIPB_37 | 88833 | 89474 | hlyIII | 0.0 | Hemolysin III | Exotoxin | 98.91 | 100.00 | NP_390062 |
| KFEIOIPB_127 | 1393 | 1842 | capC | 0.0 | Capsule | Immune modulation | 98.89 | 100.00 | NP_391470 |
| KFEIOIPB_5 | 16876 | 18072 | dhbC | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.75 | 100.00 | NP_391079 |
| KFEIOIPB_5 | 20695 | 27831 | dhbF | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.57 | 100.00 | NP_391076 |
| KFEIOIPB_5 | 18090 | 19709 | dhbE | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 97.28 | 100.00 | NP_391078 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| KFEIOIPB_29 | 27936 | 26800 | tufA | 0.0 | elongation factor Tu | 85.75 | 95.69 | AHM69299 |
| KFEIOIPB_103 | 11326 | 10457 | pdxS | 1.12e-151 | encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS | 83.79 | 98.31 | ACE61268 |
| KFEIOIPB_10 | 73984 | 74760 | CodY | 1.27e-125 | nutrient-responsive regulator | 81.08 | 100.00 | ACJ80679 |
| KFEIOIPB_48 | 96966 | 97535 | ClpP | 8.53e-100 | part of proteolytic complex | 81.05 | 97.44 | BAB94595 |
| KFEIOIPB_35 | 3080 | 3472 | spxA1 | 2.84e-71 | redox-responsive transcription factor | 83.97 | 100.00 | AAF21893 |
| KFEIOIPB_35 | 3080 | 3472 | spx | 1.69e-69 | essential gene which is a thiol/oxidative stress sensor | 80.15 | 100.00 | BAB57159 |
| KFEIOIPB_65 | 26969 | 26727 | SpoVG | 6.45e-39 | RNA-binding protein | 81.48 | 79.41 | AAT02994 |
| KFEIOIPB_17 | 75981 | 76178 | CspA | 3.36e-32 | cold shock protein | 90.91 | 100.00 | CAA62903 |
| KFEIOIPB_37 | 77602 | 77408 | CspD | 7.59e-29 | cold shock protein | 81.54 | 98.48 | CAC99957 |
| KFEIOIPB_47 | 30198 | 30004 | CspB | 7.30e-28 | cold shock protein | 83.08 | 98.48 | CAD00094 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| KFEIOIPB_1 | 0.0 | 1.0 | 0.0 |
| KFEIOIPB_2 | 0.0 | 1.0 | 0.0 |
| KFEIOIPB_3 | 0.0 | 1.0 | 0.0 |
| KFEIOIPB_4 | 0.0 | 1.0 | 0.0 |
| KFEIOIPB_5 | 0.0 | 1.0 | 0.0 |
| KFEIOIPB_6 | 0.0 | 1.0 | 0.0 |
| KFEIOIPB_7 | 0.0 | 1.0 | 0.0 |
| KFEIOIPB_8 | 0.0 | 1.0 | 0.0 |
| KFEIOIPB_9 | 0.0 | 1.0 | 0.0 |
| KFEIOIPB_10 | 0.0 | 1.0 | 0.0 |
| KFEIOIPB_11 | 0.0 | 1.0 | 0.0 |
| KFEIOIPB_12 | 0.0 | 1.0 | 0.0 |
| KFEIOIPB_13 | 0.0 | 1.0 | 0.0 |
| KFEIOIPB_14 | 0.0 | 1.0 | 0.0 |
| KFEIOIPB_15 | 0.0 | 1.0 | 0.0 |
| KFEIOIPB_16 | 0.0 | 1.0 | 0.0 |
| KFEIOIPB_17 | 0.0 | 1.0 | 0.0 |
| KFEIOIPB_18 | 0.0 | 1.0 | 0.0 |
| KFEIOIPB_19 | 0.0 | 1.0 | 0.0 |
| KFEIOIPB_20 | 0.0 | 1.0 | 0.0 |
| KFEIOIPB_21 | 0.0 | 1.0 | 0.0 |
| KFEIOIPB_22 | 0.0 | 1.0 | 0.0 |
| KFEIOIPB_23 | 0.0 | 1.0 | 0.0 |
| KFEIOIPB_24 | 0.0 | 1.0 | 0.0 |
| KFEIOIPB_25 | 0.0 | 1.0 | 0.0 |
| KFEIOIPB_26 | 0.0 | 1.0 | 0.0 |
| KFEIOIPB_27 | 0.0 | 1.0 | 0.0 |
| KFEIOIPB_28 | 0.0 | 1.0 | 0.0 |
| KFEIOIPB_29 | 0.0 | 1.0 | 0.0 |
| KFEIOIPB_30 | 0.0 | 1.0 | 0.0 |
| KFEIOIPB_31 | 0.0 | 1.0 | 0.0 |
| KFEIOIPB_32 | 0.0 | 1.0 | 0.0 |
| KFEIOIPB_33 | 0.0 | 1.0 | 0.0 |
| KFEIOIPB_34 | 0.0 | 1.0 | 0.0 |
| KFEIOIPB_35 | 0.0 | 1.0 | 0.0 |
| KFEIOIPB_36 | 0.0 | 1.0 | 0.0 |
| KFEIOIPB_37 | 0.0 | 1.0 | 0.0 |
| KFEIOIPB_38 | 0.0 | 1.0 | 0.0 |
| KFEIOIPB_39 | 0.0 | 1.0 | 0.0 |
| KFEIOIPB_40 | 0.0 | 1.0 | 0.0 |
| KFEIOIPB_41 | 0.0 | 1.0 | 0.0 |
| KFEIOIPB_43 | 0.0 | 1.0 | 0.0 |
| KFEIOIPB_44 | 0.0 | 1.0 | 0.0 |
| KFEIOIPB_45 | 0.0 | 1.0 | 0.0 |
| KFEIOIPB_46 | 0.0 | 1.0 | 0.0 |
| KFEIOIPB_47 | 0.0 | 1.0 | 0.0 |
| KFEIOIPB_48 | 0.0 | 1.0 | 0.0 |
| KFEIOIPB_49 | 0.0 | 1.0 | 0.0 |
| KFEIOIPB_50 | 0.0 | 1.0 | 0.0 |
| KFEIOIPB_51 | 0.0 | 1.0 | 0.0 |
| KFEIOIPB_52 | 0.0 | 1.0 | 0.0 |
| KFEIOIPB_53 | 0.0 | 1.0 | 0.0 |
| KFEIOIPB_55 | 0.0 | 1.0 | 0.0 |
| KFEIOIPB_56 | 0.0 | 1.0 | 0.0 |
| KFEIOIPB_57 | 0.0 | 1.0 | 0.0 |
| KFEIOIPB_58 | 0.0 | 1.0 | 0.0 |
| KFEIOIPB_59 | 0.0 | 1.0 | 0.0 |
| KFEIOIPB_60 | 0.0 | 1.0 | 0.0 |
| KFEIOIPB_61 | 0.0 | 1.0 | 0.0 |
| KFEIOIPB_62 | 0.0 | 1.0 | 0.0 |
| KFEIOIPB_63 | 1.0 | 0.0 | 1.0 |
| KFEIOIPB_64 | 1.0 | 0.0 | 1.0 |
| KFEIOIPB_65 | 0.0 | 1.0 | 0.0 |
| KFEIOIPB_66 | 0.0 | 1.0 | 0.0 |
| KFEIOIPB_67 | 0.0 | 1.0 | 0.0 |
| KFEIOIPB_68 | 0.0 | 1.0 | 0.0 |
| KFEIOIPB_69 | 0.0 | 1.0 | 0.0 |
| KFEIOIPB_70 | 1.0 | 0.0 | 1.0 |
| KFEIOIPB_71 | 0.0 | 1.0 | 0.0 |
| KFEIOIPB_72 | 0.0 | 1.0 | 0.0 |
| KFEIOIPB_73 | 0.0 | 1.0 | 0.0 |
| KFEIOIPB_74 | 0.0 | 1.0 | 0.0 |
| KFEIOIPB_75 | 0.0 | 1.0 | 0.0 |
| KFEIOIPB_76 | 0.0 | 1.0 | 0.0 |
| KFEIOIPB_77 | 0.0 | 1.0 | 0.0 |
| KFEIOIPB_78 | 0.0 | 1.0 | 0.0 |
| KFEIOIPB_79 | 1.0 | 0.0 | 1.0 |
| KFEIOIPB_80 | 0.0 | 1.0 | 0.0 |
| KFEIOIPB_81 | 0.0 | 1.0 | 0.0 |
| KFEIOIPB_82 | 0.0 | 1.0 | 0.0 |
| KFEIOIPB_83 | 0.0 | 1.0 | 0.0 |
| KFEIOIPB_84 | 1.0 | 0.0 | 1.0 |
| KFEIOIPB_85 | 0.0 | 1.0 | 0.0 |
| KFEIOIPB_86 | 0.0 | 1.0 | 0.0 |
| KFEIOIPB_87 | 0.0 | 1.0 | 0.0 |
| KFEIOIPB_88 | 1.0 | 0.0 | 1.0 |
| KFEIOIPB_89 | 0.0 | 1.0 | 0.0 |
| KFEIOIPB_90 | 1.0 | 0.0 | 1.0 |
| KFEIOIPB_91 | 0.0 | 1.0 | 0.0 |
| KFEIOIPB_92 | 0.0 | 1.0 | 0.0 |
| KFEIOIPB_93 | 0.0 | 1.0 | 0.0 |
| KFEIOIPB_94 | 0.0 | 1.0 | 0.0 |
| KFEIOIPB_96 | 0.0 | 1.0 | 0.0 |
| KFEIOIPB_97 | 1.0 | 0.0 | 1.0 |
| KFEIOIPB_98 | 0.0 | 1.0 | 0.0 |
| KFEIOIPB_100 | 0.0 | 1.0 | 0.0 |
| KFEIOIPB_101 | 0.0 | 1.0 | 0.0 |
| KFEIOIPB_102 | 0.0 | 1.0 | 0.0 |
| KFEIOIPB_103 | 0.0 | 1.0 | 0.0 |
| KFEIOIPB_104 | 0.0 | 1.0 | 0.0 |
| KFEIOIPB_105 | 0.0 | 1.0 | 0.0 |
| KFEIOIPB_106 | 0.0 | 1.0 | 0.0 |
| KFEIOIPB_107 | 0.0 | 1.0 | 0.0 |
| KFEIOIPB_108 | 0.0 | 1.0 | 0.0 |
| KFEIOIPB_110 | 0.0 | 1.0 | 0.0 |
| KFEIOIPB_111 | 0.0 | 1.0 | 0.0 |
| KFEIOIPB_112 | 0.0 | 1.0 | 0.0 |
| KFEIOIPB_114 | 0.0 | 1.0 | 0.0 |
| KFEIOIPB_115 | 0.0 | 1.0 | 0.0 |
| KFEIOIPB_116 | 0.0 | 1.0 | 0.0 |
| KFEIOIPB_117 | 1.0 | 0.0 | 1.0 |
| KFEIOIPB_119 | 0.0 | 1.0 | 0.0 |
| KFEIOIPB_120 | 0.0 | 1.0 | 0.0 |
| KFEIOIPB_121 | 0.0 | 1.0 | 0.0 |
| KFEIOIPB_123 | 0.0 | 1.0 | 0.0 |
| KFEIOIPB_124 | 1.0 | 0.0 | 1.0 |
| KFEIOIPB_125 | 1.0 | 0.0 | 1.0 |
| KFEIOIPB_126 | 0.0 | 1.0 | 0.0 |
| KFEIOIPB_127 | 0.0 | 1.0 | 0.0 |
| KFEIOIPB_128 | 1.0 | 0.0 | 1.0 |
| KFEIOIPB_129 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| KFEIOIPB_11 | 10337 | 42780 | False | Siphoviridae | VOG6513, VOG0275, VOG4602, VOG9667, VOG9667, VOG0650, VOG4552, VOG4603, VOG8233, VOG0186, VOG4566, VOG0189, VOG4693, VOG1985, VOG0198, VOG0713, VOG4841, VOG4581, VOG1886, VOG0202, VOG4556, VOG4573, VOG4553, VOG5361, VOG0204, VOG4589, VOG0539, VOG0704, VOG4586, VOG0541, VOG5910, VOG6163, VOG0801 |
| KFEIOIPB_48 | 3158 | 11498 | True | Siphoviridae | VOG9667, VOG9667, VOG1563, VOG0275, VOG0275, VOG3490 |
| KFEIOIPB_49 | 114630 | 142072 | False | Myoviridae | VOG0753, VOG4705, VOG7718, VOG4862, VOG4691, VOG4550, VOG3894, VOG4885, VOG4865, VOG6163, VOG3892, VOG3891, VOG3890, VOG0749, VOG4685, VOG4773, VOG10227, VOG0796, VOG5447, VOG4861, VOG6875, VOG0189, VOG3490, VOG9667 |
Phigaro v2.4.0 (Database: Jan. 2024 release)