Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 12 | 10 | 9 | 18.03 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| JCJLAJDH_4 | 62274 | 62591 | ykkD | ARO:3003064 | ykkD | 98.10 | 100.00 | AL009126.1 |
| JCJLAJDH_4 | 61936 | 62274 | ykkC | ARO:3003063 | ykkC | 98.21 | 100.00 | AL009126.1 |
| JCJLAJDH_16 | 46431 | 47024 | tmrB | ARO:3003059 | tmrB | 100.00 | 100.00 | AL009126.3 |
| JCJLAJDH_27 | 28569 | 30212 | vmlR | ARO:3004476 | vmlR | 98.32 | 100.00 | NC_000964.3 |
| JCJLAJDH_44 | 27371 | 29941 | Bacillus subtilis mprF | ARO:3003324 | Bsub_mprF | 99.77 | 100.00 | AL009126.3 |
| JCJLAJDH_31 | 20873 | 22042 | bmr | ARO:3003007 | bmr | 99.49 | 100.00 | M33768.1 |
| JCJLAJDH_45 | 7704 | 8555 | aadK | ARO:3002627 | aadK | 98.24 | 99.65 | AL009126.1 |
| JCJLAJDH_66 | 6846 | 8048 | blt | ARO:3003006 | blt | 99.25 | 100.00 | L32599.1 |
| JCJLAJDH_15 | 6414 | 7334 | mphK | ARO:3004541 | mphK | 98.68 | 100.00 | CP010314.1 |
| JCJLAJDH_67 | 5853 | 7286 | lmrB | ARO:3002813 | lmrB | 99.16 | 100.00 | JYFL01000006.1 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| JCJLAJDH_45 | 7704 | 8555 | aadK | Streptomycin | 98.48 | 99.65 | M26879 |
| JCJLAJDH_15 | 6414 | 7334 | mph(K) | Spiramycin, Telithromycin | 98.05 | 100.00 | NC_000964 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| JCJLAJDH_15 | 6426 | 7334 | mphK | macrolide 2'-phosphotransferase MphK | BLASTX | 98.68 | 99.02 | WP_003246254.1 |
| JCJLAJDH_45 | 7707 | 8555 | aadK | aminoglycoside 6-adenylyltransferase AadK | BLASTX | 98.24 | 100.00 | WP_003229862.1 |
| JCJLAJDH_27 | 28569 | 30209 | vmlR | ABC-F type ribosomal protection protein VmlR | BLASTX | 96.71 | 99.82 | WP_003234144.1 |
| JCJLAJDH_24 | 17447 | 17839 | satA | streptothricin N-acetyltransferase SatA | PARTIALX | 91.60 | 75.72 | WP_003242546.1 |
| JCJLAJDH_11 | 5353 | 7950 | rphC | rifamycin-inactivating phosphotransferase RphC | BLASTX | 80.48 | 100.00 | WP_087347987.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| JCJLAJDH_97 | 697 | 1878 | capB | 0.0 | Capsule | Immune modulation | 99.66 | 100.00 | NP_391471 |
| JCJLAJDH_70 | 107992 | 108777 | dhbA | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.36 | 100.00 | NP_391080 |
| JCJLAJDH_97 | 2361 | 3503 | capA | 0.0 | Capsule | Immune modulation | 99.30 | 100.00 | NP_391469 |
| JCJLAJDH_11 | 59037 | 60800 | dep/capD | 0.0 | Capsule | Immune modulation | 98.98 | 100.00 | NP_389723 |
| JCJLAJDH_70 | 104167 | 105105 | dhbB | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.94 | 100.00 | NP_391077 |
| JCJLAJDH_39 | 75042 | 75683 | hlyIII | 0.0 | Hemolysin III | Exotoxin | 98.91 | 100.00 | NP_390062 |
| JCJLAJDH_97 | 1893 | 2342 | capC | 0.0 | Capsule | Immune modulation | 98.89 | 100.00 | NP_391470 |
| JCJLAJDH_70 | 106770 | 107966 | dhbC | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.75 | 100.00 | NP_391079 |
| JCJLAJDH_70 | 97011 | 104147 | dhbF | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.57 | 100.00 | NP_391076 |
| JCJLAJDH_70 | 105133 | 106752 | dhbE | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 97.28 | 100.00 | NP_391078 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| JCJLAJDH_33 | 28113 | 26977 | tufA | 0.0 | elongation factor Tu | 85.75 | 95.69 | AHM69299 |
| JCJLAJDH_37 | 11142 | 10273 | pdxS | 1.11e-151 | encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS | 83.79 | 98.31 | ACE61268 |
| JCJLAJDH_40 | 82847 | 82071 | CodY | 1.27e-125 | nutrient-responsive regulator | 81.08 | 100.00 | ACJ80679 |
| JCJLAJDH_1 | 45202 | 44633 | ClpP | 7.57e-100 | part of proteolytic complex | 81.05 | 97.44 | BAB94595 |
| JCJLAJDH_17 | 23495 | 23887 | spxA1 | 4.14e-71 | redox-responsive transcription factor | 83.97 | 100.00 | AAF21893 |
| JCJLAJDH_17 | 23495 | 23887 | spx | 2.47e-69 | essential gene which is a thiol/oxidative stress sensor | 80.15 | 100.00 | BAB57159 |
| JCJLAJDH_72 | 20630 | 20872 | SpoVG | 6.50e-39 | RNA-binding protein | 81.48 | 79.41 | AAT02994 |
| JCJLAJDH_34 | 75984 | 76181 | CspA | 3.37e-32 | cold shock protein | 90.91 | 100.00 | CAA62903 |
| JCJLAJDH_39 | 86914 | 87108 | CspD | 1.01e-28 | cold shock protein | 81.54 | 98.48 | CAC99957 |
| JCJLAJDH_47 | 49445 | 49639 | CspB | 7.33e-28 | cold shock protein | 83.08 | 98.48 | CAD00094 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| JCJLAJDH_1 | 0.0 | 1.0 | 0.0 |
| JCJLAJDH_2 | 0.0 | 1.0 | 0.0 |
| JCJLAJDH_3 | 0.0 | 1.0 | 0.0 |
| JCJLAJDH_4 | 0.0 | 1.0 | 0.0 |
| JCJLAJDH_5 | 0.0 | 1.0 | 0.0 |
| JCJLAJDH_6 | 0.0 | 1.0 | 0.0 |
| JCJLAJDH_7 | 0.0 | 1.0 | 0.0 |
| JCJLAJDH_8 | 0.0 | 1.0 | 0.0 |
| JCJLAJDH_9 | 0.0 | 1.0 | 0.0 |
| JCJLAJDH_10 | 0.0 | 1.0 | 0.0 |
| JCJLAJDH_11 | 0.0 | 1.0 | 0.0 |
| JCJLAJDH_12 | 0.0 | 1.0 | 0.0 |
| JCJLAJDH_13 | 0.0 | 1.0 | 0.0 |
| JCJLAJDH_14 | 0.0 | 1.0 | 0.0 |
| JCJLAJDH_15 | 0.0 | 1.0 | 0.0 |
| JCJLAJDH_16 | 0.0 | 1.0 | 0.0 |
| JCJLAJDH_17 | 0.0 | 1.0 | 0.0 |
| JCJLAJDH_18 | 0.0 | 1.0 | 0.0 |
| JCJLAJDH_19 | 0.0 | 1.0 | 0.0 |
| JCJLAJDH_20 | 0.0 | 1.0 | 0.0 |
| JCJLAJDH_21 | 0.0 | 1.0 | 0.0 |
| JCJLAJDH_22 | 0.0 | 1.0 | 0.0 |
| JCJLAJDH_23 | 0.0 | 1.0 | 0.0 |
| JCJLAJDH_24 | 0.0 | 1.0 | 0.0 |
| JCJLAJDH_25 | 0.0 | 1.0 | 0.0 |
| JCJLAJDH_26 | 0.0 | 1.0 | 0.0 |
| JCJLAJDH_27 | 0.0 | 1.0 | 0.0 |
| JCJLAJDH_28 | 0.0 | 1.0 | 0.0 |
| JCJLAJDH_29 | 0.0 | 1.0 | 0.0 |
| JCJLAJDH_30 | 0.0 | 1.0 | 0.0 |
| JCJLAJDH_31 | 0.0 | 1.0 | 0.0 |
| JCJLAJDH_32 | 0.0 | 1.0 | 0.0 |
| JCJLAJDH_33 | 0.0 | 1.0 | 0.0 |
| JCJLAJDH_34 | 0.0 | 1.0 | 0.0 |
| JCJLAJDH_35 | 0.0 | 1.0 | 0.0 |
| JCJLAJDH_36 | 0.0 | 1.0 | 0.0 |
| JCJLAJDH_37 | 0.0 | 1.0 | 0.0 |
| JCJLAJDH_38 | 0.0 | 1.0 | 0.0 |
| JCJLAJDH_39 | 0.0 | 1.0 | 0.0 |
| JCJLAJDH_40 | 0.0 | 1.0 | 0.0 |
| JCJLAJDH_41 | 0.0 | 1.0 | 0.0 |
| JCJLAJDH_42 | 0.0 | 1.0 | 0.0 |
| JCJLAJDH_43 | 1.0 | 0.0 | 1.0 |
| JCJLAJDH_44 | 0.0 | 1.0 | 0.0 |
| JCJLAJDH_45 | 0.0 | 1.0 | 0.0 |
| JCJLAJDH_46 | 0.0 | 1.0 | 0.0 |
| JCJLAJDH_47 | 0.0 | 1.0 | 0.0 |
| JCJLAJDH_48 | 0.0 | 1.0 | 0.0 |
| JCJLAJDH_49 | 0.0 | 1.0 | 0.0 |
| JCJLAJDH_50 | 0.0 | 1.0 | 0.0 |
| JCJLAJDH_51 | 0.0 | 1.0 | 0.0 |
| JCJLAJDH_52 | 0.0 | 1.0 | 0.0 |
| JCJLAJDH_53 | 0.0 | 1.0 | 0.0 |
| JCJLAJDH_54 | 0.0 | 1.0 | 0.0 |
| JCJLAJDH_55 | 0.0 | 1.0 | 0.0 |
| JCJLAJDH_58 | 0.0 | 1.0 | 0.0 |
| JCJLAJDH_59 | 0.0 | 1.0 | 0.0 |
| JCJLAJDH_60 | 0.0 | 1.0 | 0.0 |
| JCJLAJDH_61 | 0.0 | 1.0 | 0.0 |
| JCJLAJDH_62 | 0.0 | 1.0 | 0.0 |
| JCJLAJDH_63 | 0.0 | 1.0 | 0.0 |
| JCJLAJDH_64 | 0.0 | 1.0 | 0.0 |
| JCJLAJDH_66 | 0.0 | 1.0 | 0.0 |
| JCJLAJDH_67 | 0.0 | 1.0 | 0.0 |
| JCJLAJDH_68 | 0.0 | 1.0 | 0.0 |
| JCJLAJDH_69 | 1.0 | 0.0 | 1.0 |
| JCJLAJDH_70 | 0.0 | 1.0 | 0.0 |
| JCJLAJDH_71 | 0.0 | 1.0 | 0.0 |
| JCJLAJDH_72 | 0.0 | 1.0 | 0.0 |
| JCJLAJDH_73 | 0.0 | 1.0 | 0.0 |
| JCJLAJDH_74 | 0.0 | 1.0 | 0.0 |
| JCJLAJDH_75 | 0.0 | 1.0 | 0.0 |
| JCJLAJDH_76 | 0.0 | 1.0 | 0.0 |
| JCJLAJDH_77 | 0.0 | 1.0 | 0.0 |
| JCJLAJDH_80 | 0.0 | 1.0 | 0.0 |
| JCJLAJDH_81 | 1.0 | 0.0 | 1.0 |
| JCJLAJDH_82 | 0.0 | 1.0 | 0.0 |
| JCJLAJDH_83 | 0.0 | 1.0 | 0.0 |
| JCJLAJDH_84 | 0.0 | 1.0 | 0.0 |
| JCJLAJDH_85 | 0.0 | 1.0 | 0.0 |
| JCJLAJDH_86 | 0.0 | 1.0 | 0.0 |
| JCJLAJDH_87 | 0.0 | 1.0 | 0.0 |
| JCJLAJDH_88 | 0.0 | 1.0 | 0.0 |
| JCJLAJDH_89 | 0.0 | 1.0 | 0.0 |
| JCJLAJDH_90 | 1.0 | 0.0 | 1.0 |
| JCJLAJDH_91 | 0.0 | 1.0 | 0.0 |
| JCJLAJDH_92 | 0.0 | 1.0 | 0.0 |
| JCJLAJDH_93 | 0.0 | 1.0 | 0.0 |
| JCJLAJDH_95 | 0.0 | 1.0 | 0.0 |
| JCJLAJDH_96 | 0.0 | 1.0 | 0.0 |
| JCJLAJDH_97 | 0.0 | 1.0 | 0.0 |
| JCJLAJDH_98 | 0.0 | 1.0 | 0.0 |
| JCJLAJDH_99 | 0.0 | 1.0 | 0.0 |
| JCJLAJDH_100 | 0.0 | 1.0 | 0.0 |
| JCJLAJDH_101 | 0.0 | 1.0 | 0.0 |
| JCJLAJDH_102 | 0.0 | 1.0 | 0.0 |
| JCJLAJDH_103 | 0.0 | 1.0 | 0.0 |
| JCJLAJDH_104 | 0.0 | 1.0 | 0.0 |
| JCJLAJDH_105 | 0.0 | 1.0 | 0.0 |
| JCJLAJDH_106 | 0.0 | 1.0 | 0.0 |
| JCJLAJDH_107 | 0.0 | 1.0 | 0.0 |
| JCJLAJDH_108 | 0.0 | 1.0 | 0.0 |
| JCJLAJDH_109 | 0.0 | 1.0 | 0.0 |
| JCJLAJDH_111 | 0.0 | 1.0 | 0.0 |
| JCJLAJDH_113 | 0.0 | 1.0 | 0.0 |
| JCJLAJDH_114 | 0.0 | 1.0 | 0.0 |
| JCJLAJDH_115 | 0.0 | 1.0 | 0.0 |
| JCJLAJDH_116 | 1.0 | 0.0 | 1.0 |
| JCJLAJDH_117 | 0.0 | 1.0 | 0.0 |
| JCJLAJDH_119 | 0.0 | 1.0 | 0.0 |
| JCJLAJDH_120 | 0.0 | 1.0 | 0.0 |
| JCJLAJDH_121 | 0.0 | 1.0 | 0.0 |
| JCJLAJDH_122 | 0.0 | 1.0 | 0.0 |
| JCJLAJDH_124 | 0.0 | 1.0 | 0.0 |
| JCJLAJDH_125 | 1.0 | 0.0 | 1.0 |
| JCJLAJDH_128 | 0.0 | 1.0 | 0.0 |
| JCJLAJDH_129 | 1.0 | 0.0 | 1.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| JCJLAJDH_4 | 5030 | 32472 | False | Myoviridae | VOG4602, VOG9667, VOG3490, VOG0189, VOG6875, VOG4861, VOG5447, VOG0796, VOG10227, VOG4773, VOG4685, VOG0749, VOG3890, VOG3891, VOG3892, VOG6163, VOG4865, VOG4885, VOG3894, VOG4550, VOG4691, VOG4862, VOG7718, VOG4705 |
| JCJLAJDH_8 | 10423 | 42866 | False | Siphoviridae | VOG6513, VOG0275, VOG4602, VOG9667, VOG9667, VOG0650, VOG4552, VOG4603, VOG8233, VOG0186, VOG4566, VOG0189, VOG4693, VOG1985, VOG0198, VOG0713, VOG4841, VOG4581, VOG1886, VOG0202, VOG4556, VOG4573, VOG4553, VOG5361, VOG0204, VOG4589, VOG0539, VOG0704, VOG4586, VOG0541, VOG5910, VOG6163, VOG0801 |
Phigaro v2.4.0 (Database: Jan. 2024 release)