Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 10 | 10 | 9 | 16.76 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| LJDIIDEM_42 | 59485 | 62055 | Bacillus subtilis mprF | ARO:3003324 | Bsub_mprF | 99.53 | 100.00 | AL009126.3 |
| LJDIIDEM_18 | 54801 | 55118 | ykkD | ARO:3003064 | ykkD | 98.10 | 100.00 | AL009126.1 |
| LJDIIDEM_18 | 54463 | 54801 | ykkC | ARO:3003063 | ykkC | 98.21 | 100.00 | AL009126.1 |
| LJDIIDEM_40 | 48067 | 49506 | lmrB | ARO:3002813 | lmrB | 99.58 | 100.42 | JYFL01000006.1 |
| LJDIIDEM_40 | 35252 | 36172 | mphK | ARO:3004541 | mphK | 98.68 | 100.00 | CP010314.1 |
| LJDIIDEM_61 | 28714 | 30360 | vmlR | ARO:3004476 | vmlR | 98.36 | 100.18 | NC_000964.3 |
| LJDIIDEM_19 | 13856 | 14710 | aadK | ARO:3002627 | aadK | 98.59 | 100.00 | AL009126.1 |
| LJDIIDEM_32 | 6981 | 8183 | blt | ARO:3003006 | blt | 98.00 | 100.00 | L32599.1 |
| LJDIIDEM_22 | 872 | 1465 | tmrB | ARO:3003059 | tmrB | 99.49 | 100.00 | AL009126.3 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| LJDIIDEM_19 | 13856 | 14710 | aadK | Streptomycin | 98.83 | 100.00 | M26879 |
| LJDIIDEM_40 | 35252 | 36172 | mph(K) | Spiramycin, Telithromycin | 98.05 | 100.00 | NC_000964 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| LJDIIDEM_40 | 35252 | 36160 | mphK | macrolide 2'-phosphotransferase MphK | BLASTX | 98.68 | 99.02 | WP_003246254.1 |
| LJDIIDEM_19 | 13859 | 14710 | aadK | aminoglycoside 6-adenylyltransferase AadK | BLASTX | 98.59 | 100.00 | WP_003229862.1 |
| LJDIIDEM_61 | 28714 | 30357 | vmlR | ABC-F type ribosomal protection protein VmlR | BLASTX | 98.36 | 100.00 | WP_003234144.1 |
| LJDIIDEM_2 | 40710 | 41228 | satA | streptothricin N-acetyltransferase SatA | BLASTX | 90.75 | 100.00 | WP_003242546.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| LJDIIDEM_27 | 26726 | 27907 | capB | 0.0 | Capsule | Immune modulation | 99.66 | 100.00 | NP_391471 |
| LJDIIDEM_14 | 112819 | 113604 | dhbA | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.49 | 100.00 | NP_391080 |
| LJDIIDEM_27 | 25101 | 26243 | capA | 0.0 | Capsule | Immune modulation | 99.30 | 100.00 | NP_391469 |
| LJDIIDEM_14 | 101827 | 108963 | dhbF | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.30 | 100.00 | NP_391076 |
| LJDIIDEM_62 | 1601 | 3364 | dep/capD | 0.0 | Capsule | Immune modulation | 99.04 | 100.00 | NP_389723 |
| LJDIIDEM_14 | 108983 | 109921 | dhbB | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.04 | 100.00 | NP_391077 |
| LJDIIDEM_14 | 111597 | 112793 | dhbC | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.00 | 100.00 | NP_391079 |
| LJDIIDEM_41 | 32545 | 33186 | hlyIII | 0.0 | Hemolysin III | Exotoxin | 98.91 | 100.00 | NP_390062 |
| LJDIIDEM_27 | 26262 | 26711 | capC | 0.0 | Capsule | Immune modulation | 98.89 | 100.00 | NP_391470 |
| LJDIIDEM_14 | 109949 | 111568 | dhbE | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 96.73 | 100.00 | NP_391078 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| LJDIIDEM_50 | 32145 | 33281 | tufA | 0.0 | elongation factor Tu | 84.96 | 95.69 | AHM69299 |
| LJDIIDEM_47 | 11172 | 10303 | pdxS | 1.97e-151 | encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS | 83.45 | 98.31 | ACE61268 |
| LJDIIDEM_8 | 134749 | 135525 | CodY | 1.88e-125 | nutrient-responsive regulator | 81.08 | 100.00 | ACJ80679 |
| LJDIIDEM_9 | 4527 | 5096 | ClpP | 2.97e-100 | part of proteolytic complex | 81.05 | 97.44 | BAB94595 |
| LJDIIDEM_37 | 41451 | 41059 | spxA1 | 3.02e-71 | redox-responsive transcription factor | 83.97 | 100.00 | AAF21893 |
| LJDIIDEM_37 | 41451 | 41059 | spx | 1.80e-69 | essential gene which is a thiol/oxidative stress sensor | 80.15 | 100.00 | BAB57159 |
| LJDIIDEM_4 | 34729 | 34487 | SpoVG | 7.52e-39 | RNA-binding protein | 81.48 | 79.41 | AAT02994 |
| LJDIIDEM_29 | 40575 | 40378 | CspA | 2.35e-32 | cold shock protein | 90.91 | 100.00 | CAA62903 |
| LJDIIDEM_41 | 44416 | 44610 | CspD | 1.09e-28 | cold shock protein | 81.54 | 98.48 | CAC99957 |
| LJDIIDEM_3 | 32162 | 31968 | CspB | 4.28e-28 | cold shock protein | 83.08 | 98.48 | CAD00094 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| LJDIIDEM_74 | 5513 | 5680 | Col(SD853) | 168 / 194 | 86.90 | NC015392 |
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| LJDIIDEM_1 | 0.0 | 1.0 | 0.0 |
| LJDIIDEM_2 | 0.0 | 1.0 | 0.0 |
| LJDIIDEM_3 | 0.0 | 1.0 | 0.0 |
| LJDIIDEM_4 | 0.0 | 1.0 | 0.0 |
| LJDIIDEM_5 | 0.0 | 1.0 | 0.0 |
| LJDIIDEM_6 | 0.0 | 1.0 | 0.0 |
| LJDIIDEM_7 | 0.0 | 1.0 | 0.0 |
| LJDIIDEM_8 | 0.0 | 1.0 | 0.0 |
| LJDIIDEM_9 | 0.0 | 1.0 | 0.0 |
| LJDIIDEM_10 | 0.0 | 1.0 | 0.0 |
| LJDIIDEM_11 | 0.0 | 1.0 | 0.0 |
| LJDIIDEM_12 | 0.0 | 1.0 | 0.0 |
| LJDIIDEM_13 | 0.0 | 1.0 | 0.0 |
| LJDIIDEM_14 | 0.0 | 1.0 | 0.0 |
| LJDIIDEM_15 | 0.0 | 1.0 | 0.0 |
| LJDIIDEM_16 | 0.0 | 1.0 | 0.0 |
| LJDIIDEM_17 | 0.0 | 1.0 | 0.0 |
| LJDIIDEM_18 | 0.0 | 1.0 | 0.0 |
| LJDIIDEM_19 | 0.0 | 1.0 | 0.0 |
| LJDIIDEM_20 | 0.0 | 1.0 | 0.0 |
| LJDIIDEM_21 | 0.0 | 1.0 | 0.0 |
| LJDIIDEM_22 | 0.0 | 1.0 | 0.0 |
| LJDIIDEM_23 | 0.0 | 1.0 | 0.0 |
| LJDIIDEM_24 | 0.0 | 1.0 | 0.0 |
| LJDIIDEM_25 | 0.0 | 1.0 | 0.0 |
| LJDIIDEM_26 | 1.0 | 0.0 | 1.0 |
| LJDIIDEM_27 | 0.0 | 1.0 | 0.0 |
| LJDIIDEM_28 | 0.0 | 1.0 | 0.0 |
| LJDIIDEM_29 | 0.0 | 1.0 | 0.0 |
| LJDIIDEM_30 | 0.0 | 1.0 | 0.0 |
| LJDIIDEM_31 | 0.0 | 1.0 | 0.0 |
| LJDIIDEM_32 | 0.0 | 1.0 | 0.0 |
| LJDIIDEM_33 | 0.0 | 1.0 | 0.0 |
| LJDIIDEM_34 | 0.0 | 1.0 | 0.0 |
| LJDIIDEM_35 | 0.0 | 1.0 | 0.0 |
| LJDIIDEM_36 | 0.0 | 1.0 | 0.0 |
| LJDIIDEM_37 | 0.0 | 1.0 | 0.0 |
| LJDIIDEM_38 | 0.0 | 1.0 | 0.0 |
| LJDIIDEM_39 | 0.0 | 1.0 | 0.0 |
| LJDIIDEM_40 | 0.0 | 1.0 | 0.0 |
| LJDIIDEM_41 | 0.0 | 1.0 | 0.0 |
| LJDIIDEM_42 | 0.0 | 1.0 | 0.0 |
| LJDIIDEM_43 | 0.0 | 1.0 | 0.0 |
| LJDIIDEM_45 | 0.0 | 1.0 | 0.0 |
| LJDIIDEM_46 | 0.0 | 1.0 | 0.0 |
| LJDIIDEM_47 | 0.0 | 1.0 | 0.0 |
| LJDIIDEM_48 | 0.0 | 1.0 | 0.0 |
| LJDIIDEM_49 | 1.0 | 0.0 | 1.0 |
| LJDIIDEM_50 | 0.0 | 1.0 | 0.0 |
| LJDIIDEM_51 | 0.0 | 1.0 | 0.0 |
| LJDIIDEM_52 | 1.0 | 0.0 | 1.0 |
| LJDIIDEM_53 | 0.0 | 1.0 | 0.0 |
| LJDIIDEM_54 | 0.0 | 1.0 | 0.0 |
| LJDIIDEM_55 | 0.0 | 1.0 | 0.0 |
| LJDIIDEM_56 | 0.0 | 1.0 | 0.0 |
| LJDIIDEM_57 | 0.0 | 1.0 | 0.0 |
| LJDIIDEM_58 | 0.0 | 1.0 | 0.0 |
| LJDIIDEM_59 | 0.0 | 1.0 | 0.0 |
| LJDIIDEM_60 | 1.0 | 0.0 | 1.0 |
| LJDIIDEM_61 | 0.0 | 1.0 | 0.0 |
| LJDIIDEM_62 | 0.0 | 1.0 | 0.0 |
| LJDIIDEM_63 | 0.0 | 1.0 | 0.0 |
| LJDIIDEM_64 | 0.0 | 1.0 | 0.0 |
| LJDIIDEM_65 | 0.0 | 1.0 | 0.0 |
| LJDIIDEM_66 | 0.0 | 1.0 | 0.0 |
| LJDIIDEM_67 | 0.0 | 1.0 | 0.0 |
| LJDIIDEM_68 | 0.0 | 1.0 | 0.0 |
| LJDIIDEM_69 | 0.0 | 1.0 | 0.0 |
| LJDIIDEM_70 | 0.0 | 1.0 | 0.0 |
| LJDIIDEM_71 | 0.0 | 1.0 | 0.0 |
| LJDIIDEM_72 | 0.0 | 1.0 | 0.0 |
| LJDIIDEM_73 | 1.0 | 0.0 | 1.0 |
| LJDIIDEM_74 | 1.0 | 0.0 | 1.0 |
| LJDIIDEM_75 | 0.0 | 1.0 | 0.0 |
| LJDIIDEM_76 | 0.0 | 1.0 | 0.0 |
| LJDIIDEM_77 | 0.0 | 1.0 | 0.0 |
| LJDIIDEM_78 | 0.0 | 1.0 | 0.0 |
| LJDIIDEM_79 | 0.0 | 1.0 | 0.0 |
| LJDIIDEM_80 | 0.0 | 1.0 | 0.0 |
| LJDIIDEM_81 | 0.0 | 1.0 | 0.0 |
| LJDIIDEM_83 | 1.0 | 0.0 | 1.0 |
| LJDIIDEM_84 | 0.0 | 1.0 | 0.0 |
| LJDIIDEM_85 | 1.0 | 0.0 | 1.0 |
| LJDIIDEM_86 | 0.0 | 1.0 | 0.0 |
| LJDIIDEM_87 | 1.0 | 0.0 | 1.0 |
| LJDIIDEM_89 | 1.0 | 0.0 | 1.0 |
| LJDIIDEM_90 | 1.0 | 0.0 | 1.0 |
| LJDIIDEM_93 | 1.0 | 0.0 | 1.0 |
| LJDIIDEM_95 | 0.0 | 1.0 | 0.0 |
| LJDIIDEM_96 | 0.0 | 1.0 | 0.0 |
| LJDIIDEM_98 | 1.0 | 0.0 | 1.0 |
| LJDIIDEM_99 | 1.0 | 0.0 | 1.0 |
| LJDIIDEM_101 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| LJDIIDEM_18 | 2878 | 24999 | False | Myoviridae | VOG10227, VOG4773, VOG4685, VOG0749, VOG3890, VOG3891, VOG3892, VOG6163, VOG4865, VOG4885, VOG3894, VOG4550, VOG4691, VOG4862, VOG7718, VOG4705 |
| LJDIIDEM_25 | 3 | 25982 | False | Siphoviridae | VOG4626, VOG4619, VOG0801, VOG6163, VOG5910, VOG0541, VOG4586, VOG0704, VOG0539, VOG4589, VOG0204, VOG5361, VOG4553, VOG4568, VOG4556, VOG3644, VOG4544, VOG4581, VOG7403, VOG4841, VOG0198, VOG7372, VOG2752, VOG4606, VOG1227, VOG1936, VOG0535, VOG0650 |
| LJDIIDEM_36 | 92567 | 104260 | True | Siphoviridae | VOG3490, VOG0275, VOG0275, VOG1563, VOG9667, VOG9667, VOG7581, VOG4566, VOG0189, VOG6587, VOG1336 |
Phigaro v2.4.0 (Database: Jan. 2024 release)