Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 10 | 10 | 9 | 16.76 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| KPGBGNPL_38 | 107090 | 108529 | lmrB | ARO:3002813 | lmrB | 99.58 | 100.42 | JYFL01000006.1 |
| KPGBGNPL_38 | 94275 | 95195 | mphK | ARO:3004541 | mphK | 98.68 | 100.00 | CP010314.1 |
| KPGBGNPL_8 | 59573 | 62143 | Bacillus subtilis mprF | ARO:3003324 | Bsub_mprF | 99.53 | 100.00 | AL009126.3 |
| KPGBGNPL_30 | 43696 | 44013 | ykkD | ARO:3003064 | ykkD | 98.10 | 100.00 | AL009126.1 |
| KPGBGNPL_30 | 43358 | 43696 | ykkC | ARO:3003063 | ykkC | 98.21 | 100.00 | AL009126.1 |
| KPGBGNPL_53 | 24094 | 24948 | aadK | ARO:3002627 | aadK | 98.59 | 100.00 | AL009126.1 |
| KPGBGNPL_71 | 18950 | 19543 | tmrB | ARO:3003059 | tmrB | 99.49 | 100.00 | AL009126.3 |
| KPGBGNPL_53 | 6997 | 8199 | blt | ARO:3003006 | blt | 98.00 | 100.00 | L32599.1 |
| KPGBGNPL_6 | 64 | 1710 | vmlR | ARO:3004476 | vmlR | 98.36 | 100.18 | NC_000964.3 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| KPGBGNPL_53 | 24094 | 24948 | aadK | Streptomycin | 98.83 | 100.00 | M26879 |
| KPGBGNPL_38 | 94275 | 95195 | mph(K) | Spiramycin, Telithromycin | 98.05 | 100.00 | NC_000964 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| KPGBGNPL_38 | 94275 | 95183 | mphK | macrolide 2'-phosphotransferase MphK | BLASTX | 98.68 | 99.02 | WP_003246254.1 |
| KPGBGNPL_53 | 24097 | 24948 | aadK | aminoglycoside 6-adenylyltransferase AadK | BLASTX | 98.59 | 100.00 | WP_003229862.1 |
| KPGBGNPL_6 | 67 | 1710 | vmlR | ABC-F type ribosomal protection protein VmlR | BLASTX | 98.36 | 100.00 | WP_003234144.1 |
| KPGBGNPL_21 | 40710 | 41228 | satA | streptothricin N-acetyltransferase SatA | BLASTX | 90.75 | 100.00 | WP_003242546.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| KPGBGNPL_22 | 226142 | 227323 | capB | 0.0 | Capsule | Immune modulation | 99.66 | 100.00 | NP_391471 |
| KPGBGNPL_70 | 36266 | 37051 | dhbA | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.49 | 100.00 | NP_391080 |
| KPGBGNPL_22 | 227806 | 228948 | capA | 0.0 | Capsule | Immune modulation | 99.30 | 100.00 | NP_391469 |
| KPGBGNPL_70 | 25274 | 32410 | dhbF | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.30 | 100.00 | NP_391076 |
| KPGBGNPL_81 | 19850 | 21613 | dep/capD | 0.0 | Capsule | Immune modulation | 99.04 | 100.00 | NP_389723 |
| KPGBGNPL_70 | 32430 | 33368 | dhbB | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.04 | 100.00 | NP_391077 |
| KPGBGNPL_70 | 35044 | 36240 | dhbC | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.00 | 100.00 | NP_391079 |
| KPGBGNPL_31 | 32626 | 33267 | hlyIII | 0.0 | Hemolysin III | Exotoxin | 98.91 | 100.00 | NP_390062 |
| KPGBGNPL_22 | 227338 | 227787 | capC | 0.0 | Capsule | Immune modulation | 98.89 | 100.00 | NP_391470 |
| KPGBGNPL_70 | 33396 | 35015 | dhbE | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 96.73 | 100.00 | NP_391078 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| KPGBGNPL_66 | 32262 | 33398 | tufA | 0.0 | elongation factor Tu | 84.96 | 95.69 | AHM69299 |
| KPGBGNPL_9 | 4496 | 5365 | pdxS | 1.99e-151 | encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS | 83.45 | 98.31 | ACE61268 |
| KPGBGNPL_12 | 151348 | 152124 | CodY | 1.99e-125 | nutrient-responsive regulator | 81.08 | 100.00 | ACJ80679 |
| KPGBGNPL_3 | 45128 | 44559 | ClpP | 2.96e-100 | part of proteolytic complex | 81.05 | 97.44 | BAB94595 |
| KPGBGNPL_41 | 23433 | 23825 | spxA1 | 4.14e-71 | redox-responsive transcription factor | 83.97 | 100.00 | AAF21893 |
| KPGBGNPL_41 | 23433 | 23825 | spx | 2.47e-69 | essential gene which is a thiol/oxidative stress sensor | 80.15 | 100.00 | BAB57159 |
| KPGBGNPL_19 | 20518 | 20760 | SpoVG | 7.51e-39 | RNA-binding protein | 81.48 | 79.41 | AAT02994 |
| KPGBGNPL_45 | 40461 | 40264 | CspA | 2.35e-32 | cold shock protein | 90.91 | 100.00 | CAA62903 |
| KPGBGNPL_31 | 44497 | 44691 | CspD | 1.09e-28 | cold shock protein | 81.54 | 98.48 | CAC99957 |
| KPGBGNPL_7 | 32200 | 32006 | CspB | 4.55e-28 | cold shock protein | 83.08 | 98.48 | CAD00094 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| KPGBGNPL_48 | 4843 | 5010 | Col(SD853) | 168 / 194 | 86.90 | NC015392 |
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| KPGBGNPL_1 | 0.0 | 1.0 | 0.0 |
| KPGBGNPL_2 | 0.0 | 1.0 | 0.0 |
| KPGBGNPL_3 | 0.0 | 1.0 | 0.0 |
| KPGBGNPL_4 | 0.0 | 1.0 | 0.0 |
| KPGBGNPL_5 | 1.0 | 0.0 | 1.0 |
| KPGBGNPL_6 | 0.0 | 1.0 | 0.0 |
| KPGBGNPL_7 | 0.0 | 1.0 | 0.0 |
| KPGBGNPL_8 | 0.0 | 1.0 | 0.0 |
| KPGBGNPL_9 | 0.0 | 1.0 | 0.0 |
| KPGBGNPL_10 | 0.0 | 1.0 | 0.0 |
| KPGBGNPL_11 | 0.0 | 1.0 | 0.0 |
| KPGBGNPL_12 | 0.0 | 1.0 | 0.0 |
| KPGBGNPL_13 | 0.0 | 1.0 | 0.0 |
| KPGBGNPL_14 | 0.0 | 1.0 | 0.0 |
| KPGBGNPL_15 | 0.0 | 1.0 | 0.0 |
| KPGBGNPL_16 | 0.0 | 1.0 | 0.0 |
| KPGBGNPL_17 | 0.0 | 1.0 | 0.0 |
| KPGBGNPL_18 | 0.0 | 1.0 | 0.0 |
| KPGBGNPL_19 | 0.0 | 1.0 | 0.0 |
| KPGBGNPL_20 | 0.0 | 1.0 | 0.0 |
| KPGBGNPL_21 | 0.0 | 1.0 | 0.0 |
| KPGBGNPL_22 | 0.0 | 1.0 | 0.0 |
| KPGBGNPL_23 | 0.0 | 1.0 | 0.0 |
| KPGBGNPL_24 | 0.0 | 1.0 | 0.0 |
| KPGBGNPL_25 | 0.0 | 1.0 | 0.0 |
| KPGBGNPL_26 | 0.0 | 1.0 | 0.0 |
| KPGBGNPL_27 | 0.0 | 1.0 | 0.0 |
| KPGBGNPL_29 | 0.0 | 1.0 | 0.0 |
| KPGBGNPL_30 | 0.0 | 1.0 | 0.0 |
| KPGBGNPL_31 | 0.0 | 1.0 | 0.0 |
| KPGBGNPL_32 | 0.0 | 1.0 | 0.0 |
| KPGBGNPL_33 | 1.0 | 0.0 | 1.0 |
| KPGBGNPL_34 | 0.0 | 1.0 | 0.0 |
| KPGBGNPL_35 | 0.0 | 1.0 | 0.0 |
| KPGBGNPL_36 | 0.0 | 1.0 | 0.0 |
| KPGBGNPL_37 | 0.0 | 1.0 | 0.0 |
| KPGBGNPL_38 | 0.0 | 1.0 | 0.0 |
| KPGBGNPL_39 | 0.0 | 1.0 | 0.0 |
| KPGBGNPL_40 | 1.0 | 0.0 | 1.0 |
| KPGBGNPL_41 | 0.0 | 1.0 | 0.0 |
| KPGBGNPL_42 | 0.0 | 1.0 | 0.0 |
| KPGBGNPL_43 | 0.0 | 1.0 | 0.0 |
| KPGBGNPL_44 | 0.0 | 1.0 | 0.0 |
| KPGBGNPL_45 | 0.0 | 1.0 | 0.0 |
| KPGBGNPL_46 | 0.0 | 1.0 | 0.0 |
| KPGBGNPL_47 | 0.0 | 1.0 | 0.0 |
| KPGBGNPL_48 | 1.0 | 0.0 | 1.0 |
| KPGBGNPL_49 | 0.0 | 1.0 | 0.0 |
| KPGBGNPL_50 | 0.0 | 1.0 | 0.0 |
| KPGBGNPL_51 | 1.0 | 0.0 | 1.0 |
| KPGBGNPL_52 | 0.0 | 1.0 | 0.0 |
| KPGBGNPL_53 | 0.0 | 1.0 | 0.0 |
| KPGBGNPL_54 | 1.0 | 0.0 | 1.0 |
| KPGBGNPL_55 | 0.0 | 1.0 | 0.0 |
| KPGBGNPL_56 | 1.0 | 0.0 | 1.0 |
| KPGBGNPL_57 | 0.0 | 1.0 | 0.0 |
| KPGBGNPL_58 | 1.0 | 0.0 | 1.0 |
| KPGBGNPL_59 | 0.0 | 1.0 | 0.0 |
| KPGBGNPL_60 | 0.0 | 1.0 | 0.0 |
| KPGBGNPL_61 | 0.0 | 1.0 | 0.0 |
| KPGBGNPL_62 | 0.0 | 1.0 | 0.0 |
| KPGBGNPL_63 | 0.0 | 1.0 | 0.0 |
| KPGBGNPL_64 | 0.0 | 1.0 | 0.0 |
| KPGBGNPL_65 | 1.0 | 0.0 | 1.0 |
| KPGBGNPL_66 | 0.0 | 1.0 | 0.0 |
| KPGBGNPL_67 | 0.0 | 1.0 | 0.0 |
| KPGBGNPL_68 | 0.0 | 1.0 | 0.0 |
| KPGBGNPL_69 | 0.0 | 1.0 | 0.0 |
| KPGBGNPL_70 | 0.0 | 1.0 | 0.0 |
| KPGBGNPL_71 | 0.0 | 1.0 | 0.0 |
| KPGBGNPL_72 | 0.0 | 1.0 | 0.0 |
| KPGBGNPL_73 | 0.0 | 1.0 | 0.0 |
| KPGBGNPL_74 | 0.0 | 1.0 | 0.0 |
| KPGBGNPL_75 | 1.0 | 0.0 | 1.0 |
| KPGBGNPL_76 | 0.0 | 1.0 | 0.0 |
| KPGBGNPL_77 | 0.0 | 1.0 | 0.0 |
| KPGBGNPL_78 | 0.0 | 1.0 | 0.0 |
| KPGBGNPL_79 | 0.0 | 1.0 | 0.0 |
| KPGBGNPL_80 | 0.0 | 1.0 | 0.0 |
| KPGBGNPL_81 | 0.0 | 1.0 | 0.0 |
| KPGBGNPL_82 | 0.0 | 1.0 | 0.0 |
| KPGBGNPL_83 | 1.0 | 0.0 | 1.0 |
| KPGBGNPL_84 | 0.0 | 1.0 | 0.0 |
| KPGBGNPL_85 | 1.0 | 0.0 | 1.0 |
| KPGBGNPL_86 | 0.0 | 1.0 | 0.0 |
| KPGBGNPL_88 | 0.0 | 1.0 | 0.0 |
| KPGBGNPL_90 | 0.0 | 1.0 | 0.0 |
| KPGBGNPL_91 | 0.0 | 1.0 | 0.0 |
| KPGBGNPL_92 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| KPGBGNPL_20 | 92567 | 103097 | True | Siphoviridae | VOG3490, VOG0275, VOG0275, VOG1563, VOG9667, VOG9667, VOG7581, VOG4566, VOG0189, VOG6587 |
| KPGBGNPL_30 | 5799 | 13572 | False | Myoviridae | VOG4865, VOG4885, VOG3894, VOG4550, VOG4691, VOG4862, VOG7718, VOG4705 |
Phigaro v2.4.0 (Database: Jan. 2024 release)