Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 10 | 10 | 9 | 16.76 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| ADIFGOFN_36 | 62272 | 62589 | ykkD | ARO:3003064 | ykkD | 98.10 | 100.00 | AL009126.1 |
| ADIFGOFN_36 | 61934 | 62272 | ykkC | ARO:3003063 | ykkC | 98.21 | 100.00 | AL009126.1 |
| ADIFGOFN_7 | 59564 | 62134 | Bacillus subtilis mprF | ARO:3003324 | Bsub_mprF | 99.53 | 100.00 | AL009126.3 |
| ADIFGOFN_10 | 41001 | 41921 | mphK | ARO:3004541 | mphK | 98.68 | 100.00 | CP010314.1 |
| ADIFGOFN_10 | 27667 | 29106 | lmrB | ARO:3002813 | lmrB | 99.58 | 100.42 | JYFL01000006.1 |
| ADIFGOFN_24 | 24117 | 24971 | aadK | ARO:3002627 | aadK | 98.59 | 100.00 | AL009126.1 |
| ADIFGOFN_55 | 19026 | 19619 | tmrB | ARO:3003059 | tmrB | 99.49 | 100.00 | AL009126.3 |
| ADIFGOFN_24 | 7020 | 8222 | blt | ARO:3003006 | blt | 98.00 | 100.00 | L32599.1 |
| ADIFGOFN_15 | 5791 | 7437 | vmlR | ARO:3004476 | vmlR | 98.36 | 100.18 | NC_000964.3 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| ADIFGOFN_24 | 24117 | 24971 | aadK | Streptomycin | 98.83 | 100.00 | M26879 |
| ADIFGOFN_10 | 41001 | 41921 | mph(K) | Spiramycin, Telithromycin | 98.05 | 100.00 | NC_000964 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| ADIFGOFN_10 | 41013 | 41921 | mphK | macrolide 2'-phosphotransferase MphK | BLASTX | 98.68 | 99.02 | WP_003246254.1 |
| ADIFGOFN_24 | 24120 | 24971 | aadK | aminoglycoside 6-adenylyltransferase AadK | BLASTX | 98.59 | 100.00 | WP_003229862.1 |
| ADIFGOFN_15 | 5794 | 7437 | vmlR | ABC-F type ribosomal protection protein VmlR | BLASTX | 98.36 | 100.00 | WP_003234144.1 |
| ADIFGOFN_52 | 51138 | 51656 | satA | streptothricin N-acetyltransferase SatA | BLASTX | 90.75 | 100.00 | WP_003242546.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| ADIFGOFN_17 | 253643 | 254824 | capB | 0.0 | Capsule | Immune modulation | 99.66 | 100.00 | NP_391471 |
| ADIFGOFN_45 | 23048 | 23833 | dhbA | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.49 | 100.00 | NP_391080 |
| ADIFGOFN_17 | 255307 | 256449 | capA | 0.0 | Capsule | Immune modulation | 99.30 | 100.00 | NP_391469 |
| ADIFGOFN_45 | 27689 | 34825 | dhbF | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.30 | 100.00 | NP_391076 |
| ADIFGOFN_68 | 1589 | 3352 | dep/capD | 0.0 | Capsule | Immune modulation | 99.04 | 100.00 | NP_389723 |
| ADIFGOFN_45 | 26731 | 27669 | dhbB | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.04 | 100.00 | NP_391077 |
| ADIFGOFN_45 | 23859 | 25055 | dhbC | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.00 | 100.00 | NP_391079 |
| ADIFGOFN_16 | 32641 | 33282 | hlyIII | 0.0 | Hemolysin III | Exotoxin | 98.91 | 100.00 | NP_390062 |
| ADIFGOFN_17 | 254839 | 255288 | capC | 0.0 | Capsule | Immune modulation | 98.89 | 100.00 | NP_391470 |
| ADIFGOFN_45 | 25084 | 26703 | dhbE | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 96.73 | 100.00 | NP_391078 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| ADIFGOFN_53 | 28041 | 26905 | tufA | 0.0 | elongation factor Tu | 84.96 | 95.69 | AHM69299 |
| ADIFGOFN_62 | 4455 | 5324 | pdxS | 2.01e-151 | encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS | 83.45 | 98.31 | ACE61268 |
| ADIFGOFN_6 | 151394 | 152170 | CodY | 2.01e-125 | nutrient-responsive regulator | 81.08 | 100.00 | ACJ80679 |
| ADIFGOFN_1 | 45213 | 44644 | ClpP | 2.97e-100 | part of proteolytic complex | 81.05 | 97.44 | BAB94595 |
| ADIFGOFN_59 | 23463 | 23855 | spxA1 | 4.13e-71 | redox-responsive transcription factor | 83.97 | 100.00 | AAF21893 |
| ADIFGOFN_59 | 23463 | 23855 | spx | 2.46e-69 | essential gene which is a thiol/oxidative stress sensor | 80.15 | 100.00 | BAB57159 |
| ADIFGOFN_57 | 217 | 459 | SpoVG | 4.73e-39 | RNA-binding protein | 81.48 | 79.41 | AAT02994 |
| ADIFGOFN_29 | 13833 | 14030 | CspA | 2.35e-32 | cold shock protein | 90.91 | 100.00 | CAA62903 |
| ADIFGOFN_16 | 44512 | 44706 | CspD | 1.09e-28 | cold shock protein | 81.54 | 98.48 | CAC99957 |
| ADIFGOFN_4 | 32005 | 31811 | CspB | 4.53e-28 | cold shock protein | 83.08 | 98.48 | CAD00094 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| ADIFGOFN_74 | 2303 | 2470 | Col(SD853) | 168 / 194 | 86.90 | NC015392 |
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| ADIFGOFN_1 | 0.0 | 1.0 | 0.0 |
| ADIFGOFN_2 | 0.0 | 1.0 | 0.0 |
| ADIFGOFN_3 | 0.0 | 1.0 | 0.0 |
| ADIFGOFN_4 | 0.0 | 1.0 | 0.0 |
| ADIFGOFN_5 | 1.0 | 0.0 | 1.0 |
| ADIFGOFN_6 | 0.0 | 1.0 | 0.0 |
| ADIFGOFN_7 | 0.0 | 1.0 | 0.0 |
| ADIFGOFN_8 | 0.0 | 1.0 | 0.0 |
| ADIFGOFN_9 | 0.0 | 1.0 | 0.0 |
| ADIFGOFN_10 | 0.0 | 1.0 | 0.0 |
| ADIFGOFN_11 | 0.0 | 1.0 | 0.0 |
| ADIFGOFN_12 | 0.0 | 1.0 | 0.0 |
| ADIFGOFN_13 | 1.0 | 0.0 | 1.0 |
| ADIFGOFN_14 | 0.0 | 1.0 | 0.0 |
| ADIFGOFN_15 | 0.0 | 1.0 | 0.0 |
| ADIFGOFN_16 | 0.0 | 1.0 | 0.0 |
| ADIFGOFN_17 | 0.0 | 1.0 | 0.0 |
| ADIFGOFN_18 | 0.0 | 1.0 | 0.0 |
| ADIFGOFN_19 | 0.0 | 1.0 | 0.0 |
| ADIFGOFN_20 | 1.0 | 0.0 | 1.0 |
| ADIFGOFN_21 | 0.0 | 1.0 | 0.0 |
| ADIFGOFN_22 | 0.0 | 1.0 | 0.0 |
| ADIFGOFN_23 | 0.0 | 1.0 | 0.0 |
| ADIFGOFN_24 | 0.0 | 1.0 | 0.0 |
| ADIFGOFN_25 | 0.0 | 1.0 | 0.0 |
| ADIFGOFN_26 | 1.0 | 0.0 | 1.0 |
| ADIFGOFN_27 | 0.0 | 1.0 | 0.0 |
| ADIFGOFN_28 | 1.0 | 0.0 | 1.0 |
| ADIFGOFN_29 | 0.0 | 1.0 | 0.0 |
| ADIFGOFN_30 | 0.0 | 1.0 | 0.0 |
| ADIFGOFN_31 | 0.0 | 1.0 | 0.0 |
| ADIFGOFN_32 | 0.0 | 1.0 | 0.0 |
| ADIFGOFN_33 | 0.0 | 1.0 | 0.0 |
| ADIFGOFN_34 | 0.0 | 1.0 | 0.0 |
| ADIFGOFN_35 | 0.0 | 1.0 | 0.0 |
| ADIFGOFN_36 | 0.0 | 1.0 | 0.0 |
| ADIFGOFN_37 | 0.0 | 1.0 | 0.0 |
| ADIFGOFN_38 | 0.0 | 1.0 | 0.0 |
| ADIFGOFN_39 | 0.0 | 1.0 | 0.0 |
| ADIFGOFN_40 | 0.0 | 1.0 | 0.0 |
| ADIFGOFN_41 | 0.0 | 1.0 | 0.0 |
| ADIFGOFN_42 | 0.0 | 1.0 | 0.0 |
| ADIFGOFN_43 | 0.0 | 1.0 | 0.0 |
| ADIFGOFN_44 | 0.0 | 1.0 | 0.0 |
| ADIFGOFN_45 | 0.0 | 1.0 | 0.0 |
| ADIFGOFN_46 | 0.0 | 1.0 | 0.0 |
| ADIFGOFN_47 | 0.0 | 1.0 | 0.0 |
| ADIFGOFN_48 | 0.0 | 1.0 | 0.0 |
| ADIFGOFN_49 | 0.0 | 1.0 | 0.0 |
| ADIFGOFN_50 | 0.0 | 1.0 | 0.0 |
| ADIFGOFN_51 | 0.0 | 1.0 | 0.0 |
| ADIFGOFN_52 | 0.0 | 1.0 | 0.0 |
| ADIFGOFN_53 | 0.0 | 1.0 | 0.0 |
| ADIFGOFN_55 | 0.0 | 1.0 | 0.0 |
| ADIFGOFN_56 | 0.0 | 1.0 | 0.0 |
| ADIFGOFN_57 | 0.0 | 1.0 | 0.0 |
| ADIFGOFN_58 | 0.0 | 1.0 | 0.0 |
| ADIFGOFN_59 | 0.0 | 1.0 | 0.0 |
| ADIFGOFN_60 | 0.0 | 1.0 | 0.0 |
| ADIFGOFN_61 | 0.0 | 1.0 | 0.0 |
| ADIFGOFN_62 | 0.0 | 1.0 | 0.0 |
| ADIFGOFN_63 | 1.0 | 0.0 | 1.0 |
| ADIFGOFN_64 | 0.0 | 1.0 | 0.0 |
| ADIFGOFN_65 | 0.0 | 1.0 | 0.0 |
| ADIFGOFN_66 | 0.0 | 1.0 | 0.0 |
| ADIFGOFN_67 | 1.0 | 0.0 | 1.0 |
| ADIFGOFN_68 | 0.0 | 1.0 | 0.0 |
| ADIFGOFN_70 | 0.0 | 1.0 | 0.0 |
| ADIFGOFN_71 | 1.0 | 0.0 | 1.0 |
| ADIFGOFN_72 | 0.0 | 1.0 | 0.0 |
| ADIFGOFN_73 | 0.0 | 1.0 | 0.0 |
| ADIFGOFN_74 | 1.0 | 0.0 | 1.0 |
| ADIFGOFN_75 | 0.0 | 1.0 | 0.0 |
| ADIFGOFN_76 | 0.0 | 1.0 | 0.0 |
| ADIFGOFN_77 | 0.0 | 1.0 | 0.0 |
| ADIFGOFN_78 | 1.0 | 0.0 | 1.0 |
| ADIFGOFN_79 | 1.0 | 0.0 | 1.0 |
| ADIFGOFN_80 | 0.0 | 1.0 | 0.0 |
| ADIFGOFN_82 | 0.0 | 1.0 | 0.0 |
| ADIFGOFN_84 | 1.0 | 0.0 | 1.0 |
| ADIFGOFN_85 | 0.0 | 1.0 | 0.0 |
| ADIFGOFN_86 | 0.0 | 1.0 | 0.0 |
| ADIFGOFN_87 | 0.0 | 1.0 | 0.0 |
| ADIFGOFN_88 | 1.0 | 0.0 | 1.0 |
| ADIFGOFN_91 | 1.0 | 0.0 | 1.0 |
| ADIFGOFN_93 | 0.0 | 1.0 | 0.0 |
| ADIFGOFN_94 | 0.0 | 1.0 | 0.0 |
| ADIFGOFN_95 | 1.0 | 0.0 | 1.0 |
| ADIFGOFN_96 | 1.0 | 0.0 | 1.0 |
| ADIFGOFN_97 | 0.0 | 1.0 | 0.0 |
| ADIFGOFN_98 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| ADIFGOFN_12 | 8195 | 18725 | True | Siphoviridae | VOG6587, VOG0189, VOG4566, VOG7581, VOG9667, VOG9667, VOG1563, VOG0275, VOG0275 |
| ADIFGOFN_36 | 5787 | 32470 | False | Myoviridae | VOG9667, VOG3490, VOG0189, VOG4843, VOG5447, VOG0796, VOG10227, VOG4773, VOG4685, VOG0749, VOG3890, VOG3891, VOG3892, VOG6163, VOG4865, VOG4885, VOG3894, VOG4550, VOG4691, VOG4862, VOG7718, VOG4705 |
Phigaro v2.4.0 (Database: Jan. 2024 release)