Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 11 | 10 | 9 | 17.38 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| AAGOJPBN_6 | 207740 | 208942 | blt | ARO:3003006 | blt | 99.25 | 100.00 | L32599.1 |
| AAGOJPBN_1 | 92731 | 95301 | Bacillus subtilis mprF | ARO:3003324 | Bsub_mprF | 99.77 | 100.00 | AL009126.3 |
| AAGOJPBN_67 | 45556 | 46995 | lmrB | ARO:3002813 | lmrB | 98.74 | 100.42 | JYFL01000006.1 |
| AAGOJPBN_6 | 34480 | 35649 | bmr | ARO:3003007 | bmr | 99.74 | 100.00 | M33768.1 |
| AAGOJPBN_58 | 19105 | 19698 | tmrB | ARO:3003059 | tmrB | 97.97 | 100.00 | AL009126.3 |
| AAGOJPBN_13 | 12809 | 14455 | vmlR | ARO:3004476 | vmlR | 98.18 | 100.18 | NC_000964.3 |
| AAGOJPBN_4 | 6542 | 6880 | ykkC | ARO:3003063 | ykkC | 100.00 | 100.00 | AL009126.1 |
| AAGOJPBN_4 | 6225 | 6542 | ykkD | ARO:3003064 | ykkD | 99.05 | 100.00 | AL009126.1 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| AAGOJPBN_67 | 32745 | 33664 | mph(K) | Spiramycin, Telithromycin | 97.72 | 99.89 | NC_000964 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| AAGOJPBN_13 | 12812 | 14455 | vmlR | ABC-F type ribosomal protection protein VmlR | BLASTX | 98.18 | 100.00 | WP_003234144.1 |
| AAGOJPBN_41 | 1159 | 1677 | satA | streptothricin N-acetyltransferase SatA | BLASTX | 91.33 | 100.00 | WP_003242546.1 |
| AAGOJPBN_89 | 518 | 3115 | rphC | rifamycin-inactivating phosphotransferase RphC | BLASTX | 80.94 | 100.00 | WP_087347987.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| AAGOJPBN_3 | 155557 | 156342 | dhbA | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.75 | 100.00 | NP_391080 |
| AAGOJPBN_7 | 266935 | 267384 | capC | 0.0 | Capsule | Immune modulation | 99.56 | 100.00 | NP_391470 |
| AAGOJPBN_7 | 267399 | 268580 | capB | 0.0 | Capsule | Immune modulation | 99.49 | 100.00 | NP_391471 |
| AAGOJPBN_27 | 166524 | 167165 | hlyIII | 0.0 | Hemolysin III | Exotoxin | 99.38 | 100.00 | NP_390062 |
| AAGOJPBN_7 | 265774 | 266916 | capA | 0.0 | Capsule | Immune modulation | 99.30 | 100.00 | NP_391469 |
| AAGOJPBN_3 | 151721 | 152659 | dhbB | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.25 | 100.00 | NP_391077 |
| AAGOJPBN_38 | 72267 | 74030 | dep/capD | 0.0 | Capsule | Immune modulation | 98.98 | 100.00 | NP_389723 |
| AAGOJPBN_3 | 144565 | 151701 | dhbF | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.70 | 100.00 | NP_391076 |
| AAGOJPBN_3 | 154335 | 155531 | dhbC | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.58 | 100.00 | NP_391079 |
| AAGOJPBN_3 | 152687 | 154306 | dhbE | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 97.53 | 100.00 | NP_391078 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| AAGOJPBN_57 | 28153 | 27017 | tufA | 0.0 | elongation factor Tu | 84.96 | 95.69 | AHM69299 |
| AAGOJPBN_52 | 11209 | 10340 | pdxS | 1.13e-151 | encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS | 83.79 | 98.31 | ACE61268 |
| AAGOJPBN_53 | 29096 | 29872 | CodY | 5.90e-126 | nutrient-responsive regulator | 81.08 | 100.00 | ACJ80679 |
| AAGOJPBN_7 | 114983 | 115552 | ClpP | 2.66e-99 | part of proteolytic complex | 81.05 | 97.44 | BAB94595 |
| AAGOJPBN_43 | 16464 | 16856 | spxA1 | 3.19e-71 | redox-responsive transcription factor | 83.97 | 100.00 | AAF21893 |
| AAGOJPBN_43 | 16464 | 16856 | spx | 1.90e-69 | essential gene which is a thiol/oxidative stress sensor | 80.15 | 100.00 | BAB57159 |
| AAGOJPBN_25 | 34986 | 34744 | SpoVG | 6.19e-39 | RNA-binding protein | 81.48 | 79.41 | AAT02994 |
| AAGOJPBN_83 | 9852 | 9655 | CspA | 6.47e-33 | cold shock protein | 90.91 | 100.00 | CAA62903 |
| AAGOJPBN_27 | 154565 | 154371 | CspD | 1.70e-28 | cold shock protein | 81.54 | 98.48 | CAC99957 |
| AAGOJPBN_26 | 229468 | 229662 | CspB | 2.40e-27 | cold shock protein | 83.08 | 98.48 | CAD00094 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| AAGOJPBN_9 | 4942 | 5135 | Col(SD853) | 194 / 194 | 97.42 | NC015392 |
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| AAGOJPBN_1 | 0.0 | 1.0 | 0.0 |
| AAGOJPBN_2 | 0.0 | 1.0 | 0.0 |
| AAGOJPBN_3 | 0.0 | 1.0 | 0.0 |
| AAGOJPBN_4 | 0.0 | 1.0 | 0.0 |
| AAGOJPBN_5 | 1.0 | 0.0 | 1.0 |
| AAGOJPBN_6 | 0.0 | 1.0 | 0.0 |
| AAGOJPBN_7 | 0.0 | 1.0 | 0.0 |
| AAGOJPBN_8 | 1.0 | 0.0 | 1.0 |
| AAGOJPBN_9 | 1.0 | 0.0 | 1.0 |
| AAGOJPBN_10 | 0.0 | 1.0 | 0.0 |
| AAGOJPBN_11 | 0.0 | 1.0 | 0.0 |
| AAGOJPBN_12 | 0.0 | 1.0 | 0.0 |
| AAGOJPBN_13 | 0.0 | 1.0 | 0.0 |
| AAGOJPBN_14 | 0.0 | 1.0 | 0.0 |
| AAGOJPBN_15 | 0.0 | 1.0 | 0.0 |
| AAGOJPBN_16 | 0.0 | 1.0 | 0.0 |
| AAGOJPBN_17 | 1.0 | 0.0 | 1.0 |
| AAGOJPBN_18 | 1.0 | 0.0 | 1.0 |
| AAGOJPBN_19 | 0.0 | 1.0 | 0.0 |
| AAGOJPBN_20 | 0.0 | 1.0 | 0.0 |
| AAGOJPBN_21 | 0.0 | 1.0 | 0.0 |
| AAGOJPBN_22 | 0.0 | 1.0 | 0.0 |
| AAGOJPBN_23 | 0.0 | 1.0 | 0.0 |
| AAGOJPBN_24 | 0.0 | 1.0 | 0.0 |
| AAGOJPBN_25 | 0.0 | 1.0 | 0.0 |
| AAGOJPBN_26 | 0.0 | 1.0 | 0.0 |
| AAGOJPBN_27 | 0.0 | 1.0 | 0.0 |
| AAGOJPBN_28 | 0.0 | 1.0 | 0.0 |
| AAGOJPBN_29 | 0.0 | 1.0 | 0.0 |
| AAGOJPBN_30 | 1.0 | 0.0 | 1.0 |
| AAGOJPBN_31 | 1.0 | 0.0 | 1.0 |
| AAGOJPBN_32 | 0.0 | 1.0 | 0.0 |
| AAGOJPBN_33 | 0.0 | 1.0 | 0.0 |
| AAGOJPBN_35 | 0.0 | 1.0 | 0.0 |
| AAGOJPBN_36 | 0.0 | 1.0 | 0.0 |
| AAGOJPBN_37 | 0.0 | 1.0 | 0.0 |
| AAGOJPBN_38 | 0.0 | 1.0 | 0.0 |
| AAGOJPBN_39 | 0.0 | 1.0 | 0.0 |
| AAGOJPBN_40 | 1.0 | 0.0 | 1.0 |
| AAGOJPBN_41 | 0.0 | 1.0 | 0.0 |
| AAGOJPBN_42 | 0.0 | 1.0 | 0.0 |
| AAGOJPBN_43 | 0.0 | 1.0 | 0.0 |
| AAGOJPBN_44 | 0.0 | 1.0 | 0.0 |
| AAGOJPBN_45 | 0.0 | 1.0 | 0.0 |
| AAGOJPBN_46 | 0.0 | 1.0 | 0.0 |
| AAGOJPBN_47 | 1.0 | 0.0 | 1.0 |
| AAGOJPBN_48 | 1.0 | 0.0 | 1.0 |
| AAGOJPBN_49 | 0.0 | 1.0 | 0.0 |
| AAGOJPBN_50 | 0.0 | 1.0 | 0.0 |
| AAGOJPBN_51 | 0.0 | 1.0 | 0.0 |
| AAGOJPBN_52 | 0.0 | 1.0 | 0.0 |
| AAGOJPBN_53 | 0.0 | 1.0 | 0.0 |
| AAGOJPBN_54 | 0.0 | 1.0 | 0.0 |
| AAGOJPBN_55 | 0.0 | 1.0 | 0.0 |
| AAGOJPBN_56 | 1.0 | 0.0 | 1.0 |
| AAGOJPBN_57 | 0.0 | 1.0 | 0.0 |
| AAGOJPBN_58 | 0.0 | 1.0 | 0.0 |
| AAGOJPBN_59 | 0.0 | 1.0 | 0.0 |
| AAGOJPBN_60 | 0.0 | 1.0 | 0.0 |
| AAGOJPBN_61 | 0.0 | 1.0 | 0.0 |
| AAGOJPBN_62 | 0.0 | 1.0 | 0.0 |
| AAGOJPBN_63 | 1.0 | 0.0 | 1.0 |
| AAGOJPBN_64 | 0.0 | 1.0 | 0.0 |
| AAGOJPBN_65 | 1.0 | 0.0 | 1.0 |
| AAGOJPBN_66 | 0.0 | 1.0 | 0.0 |
| AAGOJPBN_67 | 0.0 | 1.0 | 0.0 |
| AAGOJPBN_68 | 1.0 | 0.0 | 1.0 |
| AAGOJPBN_69 | 0.0 | 1.0 | 0.0 |
| AAGOJPBN_70 | 0.0 | 1.0 | 0.0 |
| AAGOJPBN_71 | 1.0 | 0.0 | 1.0 |
| AAGOJPBN_72 | 1.0 | 0.0 | 1.0 |
| AAGOJPBN_73 | 0.0 | 1.0 | 0.0 |
| AAGOJPBN_74 | 0.0 | 1.0 | 0.0 |
| AAGOJPBN_75 | 0.0 | 1.0 | 0.0 |
| AAGOJPBN_76 | 0.0 | 1.0 | 0.0 |
| AAGOJPBN_77 | 0.0 | 1.0 | 0.0 |
| AAGOJPBN_78 | 0.0 | 1.0 | 0.0 |
| AAGOJPBN_82 | 0.0 | 1.0 | 0.0 |
| AAGOJPBN_83 | 0.0 | 1.0 | 0.0 |
| AAGOJPBN_85 | 1.0 | 0.0 | 1.0 |
| AAGOJPBN_88 | 0.0 | 1.0 | 0.0 |
| AAGOJPBN_89 | 0.0 | 1.0 | 0.0 |
| AAGOJPBN_90 | 1.0 | 0.0 | 1.0 |
| AAGOJPBN_91 | 1.0 | 0.0 | 1.0 |
| AAGOJPBN_94 | 0.0 | 1.0 | 0.0 |
| AAGOJPBN_95 | 1.0 | 0.0 | 1.0 |
| AAGOJPBN_96 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| AAGOJPBN_2 | 16855 | 24291 | False | Siphoviridae | VOG0641, VOG7896, VOG3390, VOG4612, VOG4705, VOG3390, VOG4626, VOG1563, VOG0275 |
| AAGOJPBN_3 | 18105 | 40752 | False | Myoviridae | VOG4574, VOG0641, VOG4705, VOG3390, VOG7718, VOG4845, VOG4691, VOG4550, VOG0573, VOG1348, VOG1433, VOG1956, VOG6163, VOG1350, VOG4699, VOG1352, VOG1883, VOG1881, VOG1353, VOG4623, VOG4564, VOG4553, VOG4555, VOG0720 |
| AAGOJPBN_3 | 47938 | 54428 | False | Unknown | VOG0586, VOG0189, VOG4566, VOG1309, VOG4600, VOG1720, VOG4552 |
| AAGOJPBN_3 | 321590 | 347045 | False | Siphoviridae | VOG4574, VOG0641, VOG7896, VOG3390, VOG4612, VOG4705, VOG3390, VOG4626, VOG4772, VOG0801, VOG5910, VOG0541, VOG4586, VOG0704, VOG9583, VOG0204, VOG5361, VOG4553, VOG4568, VOG4556, VOG3607, VOG1886, VOG4581, VOG0713, VOG4841 |
| AAGOJPBN_4 | 36111 | 60648 | False | Myoviridae | VOG0136, VOG0753, VOG7895, VOG3390, VOG7718, VOG4862, VOG4691, VOG4550, VOG3894, VOG4885, VOG4865, VOG6163, VOG3892, VOG3891, VOG3890, VOG0749, VOG4685, VOG4773, VOG10227, VOG0796, VOG5447, VOG4861, VOG0189, VOG3490 |
| AAGOJPBN_27 | 193384 | 210248 | False | Siphoviridae | VOG0054, VOG8537, VOG4574, VOG4705, VOG3390, VOG4626, VOG9712, VOG5042, VOG3389, VOG4910, VOG9312 |
| AAGOJPBN_27 | 221626 | 227307 | False | Myoviridae | VOG10940, VOG5721, VOG5779 |
Phigaro v2.4.0 (Database: Jan. 2024 release)