Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 11 | 10 | 9 | 17.38 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| DFNNBLGB_7 | 159574 | 160428 | aadK | ARO:3002627 | aadK | 98.59 | 100.00 | AL009126.1 |
| DFNNBLGB_9 | 94693 | 97263 | Bacillus subtilis mprF | ARO:3003324 | Bsub_mprF | 99.65 | 100.00 | AL009126.3 |
| DFNNBLGB_20 | 70855 | 71775 | mphK | ARO:3004541 | mphK | 98.68 | 100.00 | CP010314.1 |
| DFNNBLGB_35 | 66586 | 68019 | lmrB | ARO:3002813 | lmrB | 98.95 | 100.00 | JYFL01000006.1 |
| DFNNBLGB_21 | 62267 | 62584 | ykkD | ARO:3003064 | ykkD | 98.10 | 100.00 | AL009126.1 |
| DFNNBLGB_21 | 61929 | 62267 | ykkC | ARO:3003063 | ykkC | 98.21 | 100.00 | AL009126.1 |
| DFNNBLGB_77 | 28873 | 30519 | vmlR | ARO:3004476 | vmlR | 98.36 | 100.18 | NC_000964.3 |
| DFNNBLGB_35 | 872 | 1465 | tmrB | ARO:3003059 | tmrB | 99.49 | 100.00 | AL009126.3 |
| DFNNBLGB_88 | 204 | 1406 | blt | ARO:3003006 | blt | 100.00 | 100.00 | L32599.1 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| DFNNBLGB_7 | 159574 | 160428 | aadK | Streptomycin | 98.83 | 100.00 | M26879 |
| DFNNBLGB_20 | 70855 | 71775 | mph(K) | Spiramycin, Telithromycin | 98.05 | 100.00 | NC_000964 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| DFNNBLGB_20 | 70855 | 71763 | mphK | macrolide 2'-phosphotransferase MphK | BLASTX | 98.68 | 99.02 | WP_003246254.1 |
| DFNNBLGB_7 | 159574 | 160425 | aadK | aminoglycoside 6-adenylyltransferase AadK | BLASTX | 98.59 | 100.00 | WP_003229862.1 |
| DFNNBLGB_77 | 28873 | 30516 | vmlR | ABC-F type ribosomal protection protein VmlR | BLASTX | 98.36 | 100.00 | WP_003234144.1 |
| DFNNBLGB_24 | 37386 | 37904 | satA | streptothricin N-acetyltransferase SatA | BLASTX | 91.91 | 100.00 | WP_003242546.1 |
| DFNNBLGB_38 | 135152 | 137749 | rphC | rifamycin-inactivating phosphotransferase RphC | BLASTX | 80.48 | 100.00 | WP_087347987.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| DFNNBLGB_23 | 3230 | 4411 | capB | 0.0 | Capsule | Immune modulation | 99.66 | 100.00 | NP_391471 |
| DFNNBLGB_6 | 96922 | 97707 | dhbA | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.36 | 100.00 | NP_391080 |
| DFNNBLGB_23 | 1605 | 2747 | capA | 0.0 | Capsule | Immune modulation | 99.30 | 100.00 | NP_391469 |
| DFNNBLGB_38 | 82343 | 84106 | dep/capD | 0.0 | Capsule | Immune modulation | 98.98 | 100.00 | NP_389723 |
| DFNNBLGB_32 | 75051 | 75692 | hlyIII | 0.0 | Hemolysin III | Exotoxin | 98.91 | 100.00 | NP_390062 |
| DFNNBLGB_23 | 2766 | 3215 | capC | 0.0 | Capsule | Immune modulation | 98.89 | 100.00 | NP_391470 |
| DFNNBLGB_6 | 93086 | 94024 | dhbB | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.83 | 100.00 | NP_391077 |
| DFNNBLGB_6 | 85930 | 93066 | dhbF | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.66 | 100.00 | NP_391076 |
| DFNNBLGB_6 | 95700 | 96896 | dhbC | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 97.83 | 100.00 | NP_391079 |
| DFNNBLGB_6 | 94052 | 95671 | dhbE | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 96.97 | 100.00 | NP_391078 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| DFNNBLGB_11 | 63200 | 62064 | tufA | 0.0 | elongation factor Tu | 85.75 | 95.69 | AHM69299 |
| DFNNBLGB_52 | 4250 | 5119 | pdxS | 1.11e-151 | encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS | 83.79 | 98.31 | ACE61268 |
| DFNNBLGB_8 | 74111 | 74887 | CodY | 1.27e-125 | nutrient-responsive regulator | 81.08 | 100.00 | ACJ80679 |
| DFNNBLGB_2 | 102069 | 102638 | ClpP | 9.48e-100 | part of proteolytic complex | 81.05 | 97.44 | BAB94595 |
| DFNNBLGB_59 | 23459 | 23851 | spxA1 | 2.24e-71 | redox-responsive transcription factor | 83.97 | 100.00 | AAF21893 |
| DFNNBLGB_59 | 23459 | 23851 | spx | 1.33e-69 | essential gene which is a thiol/oxidative stress sensor | 80.15 | 100.00 | BAB57159 |
| DFNNBLGB_12 | 11175 | 11417 | SpoVG | 6.22e-39 | RNA-binding protein | 81.48 | 79.41 | AAT02994 |
| DFNNBLGB_43 | 33400 | 33203 | CspA | 2.08e-32 | cold shock protein | 90.91 | 100.00 | CAA62903 |
| DFNNBLGB_32 | 86921 | 87115 | CspD | 5.41e-29 | cold shock protein | 81.54 | 98.48 | CAC99957 |
| DFNNBLGB_31 | 30065 | 29871 | CspB | 7.57e-28 | cold shock protein | 83.08 | 98.48 | CAD00094 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| DFNNBLGB_57 | 1515 | 1701 | Col(SD853) | 187 / 194 | 86.63 | NC015392 |
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| DFNNBLGB_1 | 0.0 | 1.0 | 0.0 |
| DFNNBLGB_2 | 0.0 | 1.0 | 0.0 |
| DFNNBLGB_3 | 1.0 | 0.0 | 1.0 |
| DFNNBLGB_4 | 1.0 | 0.0 | 1.0 |
| DFNNBLGB_5 | 0.0 | 1.0 | 0.0 |
| DFNNBLGB_6 | 0.0 | 1.0 | 0.0 |
| DFNNBLGB_7 | 0.0 | 1.0 | 0.0 |
| DFNNBLGB_8 | 0.0 | 1.0 | 0.0 |
| DFNNBLGB_9 | 0.0 | 1.0 | 0.0 |
| DFNNBLGB_10 | 0.0 | 1.0 | 0.0 |
| DFNNBLGB_11 | 0.0 | 1.0 | 0.0 |
| DFNNBLGB_12 | 0.0 | 1.0 | 0.0 |
| DFNNBLGB_14 | 0.0 | 1.0 | 0.0 |
| DFNNBLGB_15 | 0.0 | 1.0 | 0.0 |
| DFNNBLGB_16 | 0.0 | 1.0 | 0.0 |
| DFNNBLGB_17 | 0.0 | 1.0 | 0.0 |
| DFNNBLGB_19 | 0.0 | 1.0 | 0.0 |
| DFNNBLGB_20 | 0.0 | 1.0 | 0.0 |
| DFNNBLGB_21 | 0.0 | 1.0 | 0.0 |
| DFNNBLGB_22 | 0.0 | 1.0 | 0.0 |
| DFNNBLGB_23 | 0.0 | 1.0 | 0.0 |
| DFNNBLGB_24 | 0.0 | 1.0 | 0.0 |
| DFNNBLGB_25 | 0.0 | 1.0 | 0.0 |
| DFNNBLGB_26 | 0.0 | 1.0 | 0.0 |
| DFNNBLGB_27 | 0.0 | 1.0 | 0.0 |
| DFNNBLGB_28 | 0.0 | 1.0 | 0.0 |
| DFNNBLGB_29 | 1.0 | 0.0 | 1.0 |
| DFNNBLGB_30 | 0.0 | 1.0 | 0.0 |
| DFNNBLGB_31 | 0.0 | 1.0 | 0.0 |
| DFNNBLGB_32 | 0.0 | 1.0 | 0.0 |
| DFNNBLGB_33 | 0.0 | 1.0 | 0.0 |
| DFNNBLGB_34 | 0.0 | 1.0 | 0.0 |
| DFNNBLGB_35 | 0.0 | 1.0 | 0.0 |
| DFNNBLGB_36 | 0.0 | 1.0 | 0.0 |
| DFNNBLGB_37 | 0.0 | 1.0 | 0.0 |
| DFNNBLGB_38 | 0.0 | 1.0 | 0.0 |
| DFNNBLGB_39 | 0.0 | 1.0 | 0.0 |
| DFNNBLGB_40 | 0.0 | 1.0 | 0.0 |
| DFNNBLGB_41 | 0.0 | 1.0 | 0.0 |
| DFNNBLGB_42 | 0.0 | 1.0 | 0.0 |
| DFNNBLGB_43 | 0.0 | 1.0 | 0.0 |
| DFNNBLGB_44 | 1.0 | 0.0 | 1.0 |
| DFNNBLGB_45 | 0.0 | 1.0 | 0.0 |
| DFNNBLGB_46 | 0.0 | 1.0 | 0.0 |
| DFNNBLGB_47 | 0.0 | 1.0 | 0.0 |
| DFNNBLGB_48 | 0.0 | 1.0 | 0.0 |
| DFNNBLGB_49 | 0.0 | 1.0 | 0.0 |
| DFNNBLGB_50 | 0.0 | 1.0 | 0.0 |
| DFNNBLGB_51 | 0.0 | 1.0 | 0.0 |
| DFNNBLGB_52 | 0.0 | 1.0 | 0.0 |
| DFNNBLGB_53 | 0.0 | 1.0 | 0.0 |
| DFNNBLGB_54 | 0.0 | 1.0 | 0.0 |
| DFNNBLGB_55 | 0.0 | 1.0 | 0.0 |
| DFNNBLGB_56 | 0.0 | 1.0 | 0.0 |
| DFNNBLGB_57 | 1.0 | 0.0 | 1.0 |
| DFNNBLGB_58 | 0.0 | 1.0 | 0.0 |
| DFNNBLGB_59 | 0.0 | 1.0 | 0.0 |
| DFNNBLGB_60 | 0.0 | 1.0 | 0.0 |
| DFNNBLGB_61 | 0.0 | 1.0 | 0.0 |
| DFNNBLGB_62 | 0.0 | 1.0 | 0.0 |
| DFNNBLGB_63 | 0.0 | 1.0 | 0.0 |
| DFNNBLGB_64 | 0.0 | 1.0 | 0.0 |
| DFNNBLGB_65 | 0.0 | 1.0 | 0.0 |
| DFNNBLGB_66 | 0.0 | 1.0 | 0.0 |
| DFNNBLGB_67 | 0.0 | 1.0 | 0.0 |
| DFNNBLGB_68 | 0.0 | 1.0 | 0.0 |
| DFNNBLGB_69 | 0.0 | 1.0 | 0.0 |
| DFNNBLGB_70 | 0.0 | 1.0 | 0.0 |
| DFNNBLGB_71 | 0.0 | 1.0 | 0.0 |
| DFNNBLGB_73 | 0.0 | 1.0 | 0.0 |
| DFNNBLGB_74 | 0.0 | 1.0 | 0.0 |
| DFNNBLGB_75 | 0.0 | 1.0 | 0.0 |
| DFNNBLGB_77 | 0.0 | 1.0 | 0.0 |
| DFNNBLGB_78 | 0.0 | 1.0 | 0.0 |
| DFNNBLGB_79 | 0.0 | 1.0 | 0.0 |
| DFNNBLGB_80 | 0.0 | 1.0 | 0.0 |
| DFNNBLGB_84 | 1.0 | 0.0 | 1.0 |
| DFNNBLGB_85 | 0.0 | 1.0 | 0.0 |
| DFNNBLGB_86 | 0.0 | 1.0 | 0.0 |
| DFNNBLGB_87 | 0.0 | 1.0 | 0.0 |
| DFNNBLGB_88 | 0.0 | 1.0 | 0.0 |
| DFNNBLGB_90 | 0.0 | 1.0 | 0.0 |
| DFNNBLGB_91 | 0.0 | 1.0 | 0.0 |
| DFNNBLGB_92 | 0.0 | 1.0 | 0.0 |
| DFNNBLGB_93 | 0.0 | 1.0 | 0.0 |
| DFNNBLGB_94 | 0.0 | 1.0 | 0.0 |
| DFNNBLGB_95 | 1.0 | 0.0 | 1.0 |
| DFNNBLGB_96 | 0.0 | 1.0 | 0.0 |
| DFNNBLGB_97 | 0.0 | 1.0 | 0.0 |
| DFNNBLGB_98 | 0.0 | 1.0 | 0.0 |
| DFNNBLGB_99 | 1.0 | 0.0 | 1.0 |
| DFNNBLGB_100 | 0.0 | 1.0 | 0.0 |
| DFNNBLGB_102 | 0.0 | 1.0 | 0.0 |
| DFNNBLGB_103 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| DFNNBLGB_2 | 4946 | 15476 | True | Siphoviridae | VOG6587, VOG0189, VOG4566, VOG7581, VOG9667, VOG9667, VOG1563, VOG0275, VOG0275 |
| DFNNBLGB_11 | 8042 | 37481 | False | Siphoviridae | VOG4574, VOG0641, VOG4705, VOG6243, VOG4612, VOG4626, VOG4619, VOG0801, VOG6163, VOG5910, VOG0541, VOG4586, VOG0704, VOG0539, VOG4589, VOG0204, VOG5361, VOG4553, VOG4568, VOG4556, VOG3607, VOG1886, VOG4581, VOG7403, VOG4841, VOG0198, VOG5395, VOG0044, VOG7372, VOG10041, VOG2752, VOG7060, VOG4606, VOG1227, VOG1936 |
| DFNNBLGB_21 | 6309 | 32465 | False | Myoviridae | VOG3490, VOG0189, VOG4861, VOG5447, VOG0796, VOG10227, VOG4773, VOG4685, VOG0749, VOG3890, VOG3891, VOG3892, VOG6163, VOG4865, VOG4885, VOG3894, VOG4550, VOG4691, VOG4862, VOG7718, VOG4705 |
| DFNNBLGB_53 | 572 | 14337 | False | Siphoviridae | VOG4581, VOG1886, VOG3607, VOG4556, VOG4568, VOG4553, VOG10223, VOG0204, VOG9583, VOG0704, VOG4586, VOG0541, VOG5910 |
Phigaro v2.4.0 (Database: Jan. 2024 release)