Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 9 | 10 | 9 | 16.19 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| FLGMFOMJ_13 | 36812 | 37981 | bmr | ARO:3003007 | bmr | 98.97 | 100.00 | M33768.1 |
| FLGMFOMJ_78 | 29913 | 31352 | lmrB | ARO:3002813 | lmrB | 98.74 | 100.42 | JYFL01000006.1 |
| FLGMFOMJ_50 | 6434 | 6772 | ykkC | ARO:3003063 | ykkC | 99.11 | 100.00 | AL009126.1 |
| FLGMFOMJ_50 | 6117 | 6434 | ykkD | ARO:3003064 | ykkD | 98.10 | 100.00 | AL009126.1 |
| FLGMFOMJ_104 | 940 | 1533 | tmrB | ARO:3003059 | tmrB | 97.97 | 100.00 | AL009126.3 |
| FLGMFOMJ_89 | 688 | 2331 | vmlR | ARO:3004476 | vmlR | 97.99 | 100.00 | NC_000964.3 |
| FLGMFOMJ_20 | 179 | 2749 | Bacillus subtilis mprF | ARO:3003324 | Bsub_mprF | 99.77 | 100.00 | AL009126.3 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| FLGMFOMJ_78 | 17100 | 18021 | mph(K) | Spiramycin, Telithromycin | 97.29 | 100.00 | NC_000964 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| FLGMFOMJ_89 | 691 | 2331 | vmlR | ABC-F type ribosomal protection protein VmlR | BLASTX | 97.99 | 100.00 | WP_003234144.1 |
| FLGMFOMJ_84 | 5931 | 6317 | satA | streptothricin N-acetyltransferase SatA | INTERNAL_STOP | 86.05 | 74.57 | WP_003242546.1 |
| FLGMFOMJ_78 | 17100 | 17723 | mphK | macrolide 2'-phosphotransferase MphK | PARTIALX | 97.60 | 67.97 | WP_003246254.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| FLGMFOMJ_2 | 39811 | 40992 | capB | 0.0 | Capsule | Immune modulation | 99.66 | 100.00 | NP_391471 |
| FLGMFOMJ_2 | 41007 | 41456 | capC | 0.0 | Capsule | Immune modulation | 99.56 | 100.00 | NP_391470 |
| FLGMFOMJ_3 | 15234 | 15875 | hlyIII | 0.0 | Hemolysin III | Exotoxin | 99.53 | 100.00 | NP_390062 |
| FLGMFOMJ_2 | 41475 | 42617 | capA | 0.0 | Capsule | Immune modulation | 99.30 | 100.00 | NP_391469 |
| FLGMFOMJ_75 | 35577 | 36362 | dhbA | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.11 | 100.00 | NP_391080 |
| FLGMFOMJ_75 | 24586 | 31722 | dhbF | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.84 | 100.00 | NP_391076 |
| FLGMFOMJ_75 | 34355 | 35551 | dhbC | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.83 | 100.00 | NP_391079 |
| FLGMFOMJ_29 | 41558 | 43321 | dep/capD | 0.0 | Capsule | Immune modulation | 98.75 | 100.00 | NP_389723 |
| FLGMFOMJ_75 | 31742 | 32680 | dhbB | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.51 | 100.00 | NP_391077 |
| FLGMFOMJ_75 | 32708 | 34326 | dhbE | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 97.90 | 99.94 | NP_391078 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| FLGMFOMJ_82 | 31696 | 32832 | tufA | 0.0 | elongation factor Tu | 85.75 | 95.69 | AHM69299 |
| FLGMFOMJ_76 | 4598 | 5467 | pdxS | 1.98e-151 | encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS | 83.45 | 98.31 | ACE61268 |
| FLGMFOMJ_5 | 61015 | 60239 | CodY | 1.09e-125 | nutrient-responsive regulator | 81.08 | 100.00 | ACJ80679 |
| FLGMFOMJ_54 | 123159 | 123728 | ClpP | 9.65e-100 | part of proteolytic complex | 81.05 | 97.44 | BAB94595 |
| FLGMFOMJ_17 | 21234 | 20842 | spxA1 | 3.94e-71 | redox-responsive transcription factor | 83.97 | 100.00 | AAF21893 |
| FLGMFOMJ_17 | 21234 | 20842 | spx | 2.35e-69 | essential gene which is a thiol/oxidative stress sensor | 80.15 | 100.00 | BAB57159 |
| FLGMFOMJ_66 | 4372 | 4614 | SpoVG | 5.32e-39 | RNA-binding protein | 81.48 | 79.41 | AAT02994 |
| FLGMFOMJ_96 | 6158 | 6355 | CspA | 2.82e-33 | cold shock protein | 90.91 | 100.00 | CAA62903 |
| FLGMFOMJ_3 | 27839 | 28033 | CspD | 8.16e-29 | cold shock protein | 81.54 | 98.48 | CAC99957 |
| FLGMFOMJ_6 | 22696 | 22502 | CspB | 2.44e-28 | cold shock protein | 83.08 | 98.48 | CAD00094 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| FLGMFOMJ_74 | 3134 | 3327 | Col(SD853) | 194 / 194 | 98.97 | NC015392 |
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| FLGMFOMJ_1 | 0.0 | 1.0 | 0.0 |
| FLGMFOMJ_2 | 0.0 | 1.0 | 0.0 |
| FLGMFOMJ_3 | 0.0 | 1.0 | 0.0 |
| FLGMFOMJ_4 | 0.0 | 1.0 | 0.0 |
| FLGMFOMJ_5 | 0.0 | 1.0 | 0.0 |
| FLGMFOMJ_6 | 0.0 | 1.0 | 0.0 |
| FLGMFOMJ_7 | 0.0 | 1.0 | 0.0 |
| FLGMFOMJ_8 | 0.0 | 1.0 | 0.0 |
| FLGMFOMJ_9 | 0.0 | 1.0 | 0.0 |
| FLGMFOMJ_10 | 0.0 | 1.0 | 0.0 |
| FLGMFOMJ_11 | 0.0 | 1.0 | 0.0 |
| FLGMFOMJ_12 | 0.0 | 1.0 | 0.0 |
| FLGMFOMJ_13 | 0.0 | 1.0 | 0.0 |
| FLGMFOMJ_14 | 0.0 | 1.0 | 0.0 |
| FLGMFOMJ_15 | 0.0 | 1.0 | 0.0 |
| FLGMFOMJ_16 | 0.0 | 1.0 | 0.0 |
| FLGMFOMJ_17 | 0.0 | 1.0 | 0.0 |
| FLGMFOMJ_18 | 0.0 | 1.0 | 0.0 |
| FLGMFOMJ_19 | 0.0 | 1.0 | 0.0 |
| FLGMFOMJ_20 | 0.0 | 1.0 | 0.0 |
| FLGMFOMJ_21 | 0.0 | 1.0 | 0.0 |
| FLGMFOMJ_22 | 0.0 | 1.0 | 0.0 |
| FLGMFOMJ_23 | 0.0 | 1.0 | 0.0 |
| FLGMFOMJ_24 | 0.0 | 1.0 | 0.0 |
| FLGMFOMJ_25 | 0.0 | 1.0 | 0.0 |
| FLGMFOMJ_26 | 0.0 | 1.0 | 0.0 |
| FLGMFOMJ_27 | 0.0 | 1.0 | 0.0 |
| FLGMFOMJ_28 | 1.0 | 0.0 | 1.0 |
| FLGMFOMJ_29 | 0.0 | 1.0 | 0.0 |
| FLGMFOMJ_30 | 0.0 | 1.0 | 0.0 |
| FLGMFOMJ_31 | 1.0 | 0.0 | 1.0 |
| FLGMFOMJ_32 | 0.0 | 1.0 | 0.0 |
| FLGMFOMJ_33 | 1.0 | 0.0 | 1.0 |
| FLGMFOMJ_34 | 0.0 | 1.0 | 0.0 |
| FLGMFOMJ_35 | 0.0 | 1.0 | 0.0 |
| FLGMFOMJ_36 | 0.0 | 1.0 | 0.0 |
| FLGMFOMJ_37 | 0.0 | 1.0 | 0.0 |
| FLGMFOMJ_38 | 0.0 | 1.0 | 0.0 |
| FLGMFOMJ_39 | 0.0 | 1.0 | 0.0 |
| FLGMFOMJ_40 | 0.0 | 1.0 | 0.0 |
| FLGMFOMJ_41 | 0.0 | 1.0 | 0.0 |
| FLGMFOMJ_42 | 0.0 | 1.0 | 0.0 |
| FLGMFOMJ_43 | 0.0 | 1.0 | 0.0 |
| FLGMFOMJ_44 | 0.0 | 1.0 | 0.0 |
| FLGMFOMJ_45 | 0.0 | 1.0 | 0.0 |
| FLGMFOMJ_46 | 0.0 | 1.0 | 0.0 |
| FLGMFOMJ_47 | 0.0 | 1.0 | 0.0 |
| FLGMFOMJ_48 | 0.0 | 1.0 | 0.0 |
| FLGMFOMJ_49 | 0.0 | 1.0 | 0.0 |
| FLGMFOMJ_50 | 0.0 | 1.0 | 0.0 |
| FLGMFOMJ_51 | 0.0 | 1.0 | 0.0 |
| FLGMFOMJ_52 | 0.0 | 1.0 | 0.0 |
| FLGMFOMJ_53 | 0.0 | 1.0 | 0.0 |
| FLGMFOMJ_54 | 0.0 | 1.0 | 0.0 |
| FLGMFOMJ_57 | 0.0 | 1.0 | 0.0 |
| FLGMFOMJ_58 | 0.0 | 1.0 | 0.0 |
| FLGMFOMJ_59 | 0.0 | 1.0 | 0.0 |
| FLGMFOMJ_60 | 0.0 | 1.0 | 0.0 |
| FLGMFOMJ_61 | 0.0 | 1.0 | 0.0 |
| FLGMFOMJ_62 | 0.0 | 1.0 | 0.0 |
| FLGMFOMJ_63 | 0.0 | 1.0 | 0.0 |
| FLGMFOMJ_64 | 0.0 | 1.0 | 0.0 |
| FLGMFOMJ_66 | 0.0 | 1.0 | 0.0 |
| FLGMFOMJ_67 | 0.0 | 1.0 | 0.0 |
| FLGMFOMJ_68 | 0.0 | 1.0 | 0.0 |
| FLGMFOMJ_69 | 0.0 | 1.0 | 0.0 |
| FLGMFOMJ_70 | 0.0 | 1.0 | 0.0 |
| FLGMFOMJ_71 | 0.0 | 1.0 | 0.0 |
| FLGMFOMJ_74 | 1.0 | 0.0 | 1.0 |
| FLGMFOMJ_75 | 0.0 | 1.0 | 0.0 |
| FLGMFOMJ_76 | 0.0 | 1.0 | 0.0 |
| FLGMFOMJ_77 | 1.0 | 0.0 | 1.0 |
| FLGMFOMJ_78 | 0.0 | 1.0 | 0.0 |
| FLGMFOMJ_79 | 0.0 | 1.0 | 0.0 |
| FLGMFOMJ_80 | 0.0 | 1.0 | 0.0 |
| FLGMFOMJ_81 | 0.0 | 1.0 | 0.0 |
| FLGMFOMJ_82 | 0.0 | 1.0 | 0.0 |
| FLGMFOMJ_83 | 0.0 | 1.0 | 0.0 |
| FLGMFOMJ_84 | 0.0 | 1.0 | 0.0 |
| FLGMFOMJ_85 | 0.0 | 1.0 | 0.0 |
| FLGMFOMJ_86 | 0.0 | 1.0 | 0.0 |
| FLGMFOMJ_87 | 0.0 | 1.0 | 0.0 |
| FLGMFOMJ_89 | 0.0 | 1.0 | 0.0 |
| FLGMFOMJ_90 | 0.0 | 1.0 | 0.0 |
| FLGMFOMJ_91 | 0.0 | 1.0 | 0.0 |
| FLGMFOMJ_92 | 0.0 | 1.0 | 0.0 |
| FLGMFOMJ_93 | 0.0 | 1.0 | 0.0 |
| FLGMFOMJ_94 | 0.0 | 1.0 | 0.0 |
| FLGMFOMJ_95 | 0.0 | 1.0 | 0.0 |
| FLGMFOMJ_96 | 0.0 | 1.0 | 0.0 |
| FLGMFOMJ_98 | 0.0 | 1.0 | 0.0 |
| FLGMFOMJ_99 | 1.0 | 0.0 | 1.0 |
| FLGMFOMJ_100 | 1.0 | 0.0 | 1.0 |
| FLGMFOMJ_101 | 0.0 | 1.0 | 0.0 |
| FLGMFOMJ_102 | 0.0 | 1.0 | 0.0 |
| FLGMFOMJ_103 | 1.0 | 0.0 | 1.0 |
| FLGMFOMJ_104 | 0.0 | 1.0 | 0.0 |
| FLGMFOMJ_105 | 0.0 | 1.0 | 0.0 |
| FLGMFOMJ_106 | 0.0 | 1.0 | 0.0 |
| FLGMFOMJ_107 | 0.0 | 1.0 | 0.0 |
| FLGMFOMJ_108 | 1.0 | 0.0 | 1.0 |
| FLGMFOMJ_109 | 0.0 | 1.0 | 0.0 |
| FLGMFOMJ_110 | 0.0 | 1.0 | 0.0 |
| FLGMFOMJ_111 | 0.0 | 1.0 | 0.0 |
| FLGMFOMJ_114 | 0.0 | 1.0 | 0.0 |
| FLGMFOMJ_115 | 1.0 | 0.0 | 1.0 |
| FLGMFOMJ_116 | 0.0 | 1.0 | 0.0 |
| FLGMFOMJ_117 | 0.0 | 1.0 | 0.0 |
| FLGMFOMJ_119 | 1.0 | 0.0 | 1.0 |
| FLGMFOMJ_120 | 0.0 | 1.0 | 0.0 |
| FLGMFOMJ_121 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| FLGMFOMJ_29 | 4508 | 31543 | False | Siphoviridae | VOG0641, VOG7896, VOG3390, VOG4612, VOG4660, VOG4772, VOG0801, VOG5910, VOG0541, VOG4586, VOG0704, VOG0539, VOG1266, VOG0204, VOG5361, VOG4553, VOG4568, VOG4556, VOG3607, VOG1886, VOG4581, VOG0713, VOG4841, VOG9029, VOG0275, VOG0198, VOG5447, VOG6581 |
| FLGMFOMJ_42 | 8500 | 27772 | False | Siphoviridae | VOG5535, VOG4599, VOG4605, VOG4545, VOG0725, VOG2621, VOG0799, VOG0724, VOG5660, VOG0816, VOG4623, VOG3480, VOG1329, VOG5361, VOG4555, VOG4564, VOG0720 |
| FLGMFOMJ_50 | 36027 | 56032 | False | Myoviridae | VOG0136, VOG0753, VOG4705, VOG3390, VOG7718, VOG4862, VOG4691, VOG4550, VOG3894, VOG4885, VOG4885, VOG4865, VOG6163, VOG3892, VOG3891, VOG3890, VOG9315, VOG0796, VOG5447, VOG4861, VOG0189, VOG3490 |
| FLGMFOMJ_70 | 6694 | 10495 | False | Unknown | VOG4600, VOG4552, VOG8241 |
Phigaro v2.4.0 (Database: Jan. 2024 release)