Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 13 | 10 | 8 | 18.25 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| BCHHBOED_5 | 658665 | 659003 | ykkC | ARO:3003063 | ykkC | 100.00 | 100.00 | AL009126.1 |
| BCHHBOED_5 | 658348 | 658665 | ykkD | ARO:3003064 | ykkD | 99.05 | 100.00 | AL009126.1 |
| BCHHBOED_8 | 598894 | 599748 | aadK | ARO:3002627 | aadK | 97.89 | 100.00 | AL009126.1 |
| BCHHBOED_8 | 579575 | 580777 | blt | ARO:3003006 | blt | 99.75 | 100.00 | L32599.1 |
| BCHHBOED_8 | 406290 | 407459 | bmr | ARO:3003007 | bmr | 99.49 | 100.00 | M33768.1 |
| BCHHBOED_6 | 338451 | 339371 | mphK | ARO:3004541 | mphK | 98.37 | 100.00 | CP010314.1 |
| BCHHBOED_6 | 325119 | 326558 | lmrB | ARO:3002813 | lmrB | 99.16 | 100.42 | JYFL01000006.1 |
| BCHHBOED_6 | 275443 | 276036 | tmrB | ARO:3003059 | tmrB | 97.46 | 100.00 | AL009126.3 |
| BCHHBOED_6 | 29036 | 30679 | vmlR | ARO:3004476 | vmlR | 98.35 | 100.00 | NC_000964.3 |
| BCHHBOED_2 | 27339 | 29909 | Bacillus subtilis mprF | ARO:3003324 | Bsub_mprF | 100.00 | 100.00 | AL009126.3 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| BCHHBOED_1 | 841746 | 843122 | tet(L) | Doxycycline, Tetracycline, Minocycline, Tigecycline, Doxycycline, Tetracycline | 98.91 | 100.00 | X08034 |
| BCHHBOED_8 | 598896 | 599748 | aadK | Streptomycin | 98.48 | 99.77 | M26879 |
| BCHHBOED_6 | 338451 | 339371 | mph(K) | Spiramycin, Telithromycin | 97.83 | 100.00 | NC_000964 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| BCHHBOED_1 | 841749 | 843122 | tet(L) | tetracycline efflux MFS transporter Tet(L) | BLASTX | 98.91 | 100.00 | WP_003242953.1 |
| BCHHBOED_6 | 338454 | 339371 | mphK | macrolide 2'-phosphotransferase MphK | BLASTX | 98.37 | 100.00 | WP_003246254.1 |
| BCHHBOED_6 | 29036 | 30676 | vmlR | ABC-F type ribosomal protection protein VmlR | BLASTX | 98.35 | 100.00 | WP_003234144.1 |
| BCHHBOED_8 | 598897 | 599748 | aadK | aminoglycoside 6-adenylyltransferase AadK | BLASTX | 97.89 | 100.00 | WP_003229862.1 |
| BCHHBOED_1 | 839284 | 839802 | satA | streptothricin N-acetyltransferase SatA | BLASTX | 90.17 | 100.00 | WP_003242546.1 |
| BCHHBOED_8 | 56686 | 59283 | rphC | rifamycin-inactivating phosphotransferase RphC | BLASTX | 81.06 | 100.00 | WP_087347987.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| BCHHBOED_1 | 356204 | 357385 | capB | 0.0 | Capsule | Immune modulation | 99.58 | 100.00 | NP_391471 |
| BCHHBOED_1 | 354579 | 355721 | capA | 0.0 | Capsule | Immune modulation | 99.47 | 100.00 | NP_391469 |
| BCHHBOED_8 | 164515 | 165156 | hlyIII | 0.0 | Hemolysin III | Exotoxin | 99.38 | 100.00 | NP_390062 |
| BCHHBOED_11 | 112996 | 113781 | dhbA | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.36 | 100.00 | NP_391080 |
| BCHHBOED_11 | 109160 | 110098 | dhbB | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.36 | 100.00 | NP_391077 |
| BCHHBOED_1 | 355740 | 356189 | capC | 0.0 | Capsule | Immune modulation | 99.33 | 100.00 | NP_391470 |
| BCHHBOED_11 | 102004 | 109140 | dhbF | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.22 | 100.00 | NP_391076 |
| BCHHBOED_8 | 9736 | 11499 | dep/capD | 0.0 | Capsule | Immune modulation | 98.81 | 100.00 | NP_389723 |
| BCHHBOED_11 | 111774 | 112970 | dhbC | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.58 | 100.00 | NP_391079 |
| BCHHBOED_11 | 110126 | 111745 | dhbE | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.52 | 100.00 | NP_391078 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| BCHHBOED_17 | 31693 | 32829 | tufA | 0.0 | elongation factor Tu | 84.96 | 95.69 | AHM69299 |
| BCHHBOED_10 | 4527 | 5396 | pdxS | 1.13e-151 | encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS | 83.79 | 98.31 | ACE61268 |
| BCHHBOED_5 | 345177 | 344401 | CodY | 5.68e-125 | nutrient-responsive regulator | 81.08 | 100.00 | ACJ80679 |
| BCHHBOED_1 | 197613 | 198182 | ClpP | 5.28e-99 | part of proteolytic complex | 81.05 | 97.44 | BAB94595 |
| BCHHBOED_4 | 276366 | 276758 | spxA1 | 2.11e-70 | redox-responsive transcription factor | 83.97 | 100.00 | AAF21893 |
| BCHHBOED_4 | 276366 | 276758 | spx | 1.26e-68 | essential gene which is a thiol/oxidative stress sensor | 80.15 | 100.00 | BAB57159 |
| BCHHBOED_9 | 34785 | 34543 | SpoVG | 7.51e-39 | RNA-binding protein | 81.48 | 79.41 | AAT02994 |
| BCHHBOED_6 | 78679 | 78482 | CspA | 1.85e-31 | cold shock protein | 90.91 | 100.00 | CAA62903 |
| BCHHBOED_4 | 33155 | 32961 | CspB | 3.04e-27 | cold shock protein | 83.08 | 98.48 | CAD00094 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| BCHHBOED_14 | 3191 | 3378 | Col(SD853) | 188 / 194 | 93.62 | NC015392 |
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| BCHHBOED_1 | 0.0 | 1.0 | 0.0 |
| BCHHBOED_2 | 0.0 | 1.0 | 0.0 |
| BCHHBOED_3 | 0.0 | 1.0 | 0.0 |
| BCHHBOED_4 | 0.0 | 1.0 | 0.0 |
| BCHHBOED_5 | 0.0 | 1.0 | 0.0 |
| BCHHBOED_6 | 0.0 | 1.0 | 0.0 |
| BCHHBOED_7 | 0.0 | 1.0 | 0.0 |
| BCHHBOED_8 | 0.0 | 1.0 | 0.0 |
| BCHHBOED_9 | 0.0 | 1.0 | 0.0 |
| BCHHBOED_10 | 0.0 | 1.0 | 0.0 |
| BCHHBOED_11 | 0.0 | 1.0 | 0.0 |
| BCHHBOED_12 | 1.0 | 0.0 | 1.0 |
| BCHHBOED_13 | 1.0 | 0.0 | 1.0 |
| BCHHBOED_14 | 1.0 | 0.0 | 1.0 |
| BCHHBOED_15 | 0.0 | 1.0 | 0.0 |
| BCHHBOED_16 | 0.0 | 1.0 | 0.0 |
| BCHHBOED_17 | 0.0 | 1.0 | 0.0 |
| BCHHBOED_19 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| BCHHBOED_5 | 94973 | 106488 | False | Myoviridae / Siphoviridae | VOG4581, VOG5722, VOG5779, VOG5721, VOG10940, VOG7134, VOG6036, VOG5495, VOG9219, VOG9713 |
| BCHHBOED_5 | 114163 | 129165 | False | Unknown | VOG9312, VOG4910, VOG3389, VOG5042, VOG9712 |
| BCHHBOED_5 | 688496 | 696637 | False | Myoviridae | VOG0753, VOG4705, VOG3390, VOG7718, VOG4862, VOG4691, VOG4550, VOG3894, VOG4885 |
| BCHHBOED_8 | 195244 | 212914 | False | Siphoviridae | VOG6489, VOG5042, VOG4910, VOG9219, VOG4713, VOG4572, VOG4555, VOG0720, VOG7734, VOG0221, VOG3699 |
| BCHHBOED_8 | 768137 | 793508 | False | Myoviridae | VOG0221, VOG6116, VOG4574, VOG0136, VOG0753, VOG4705, VOG3390, VOG7718, VOG4845, VOG4691, VOG4550, VOG0573, VOG1348, VOG1433, VOG1956, VOG6163, VOG4699, VOG1352, VOG1883, VOG1881, VOG1353, VOG4623, VOG4564, VOG0815, VOG4553, VOG4555, VOG0720 |
| BCHHBOED_15 | 5503 | 15425 | False | Siphoviridae | VOG0749, VOG4685, VOG4773, VOG10227, VOG0796, VOG5447, VOG4861, VOG0189, VOG3490, VOG9667 |
Phigaro v2.4.0 (Database: Jan. 2024 release)