Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 12 | 10 | 7 | 17.12 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| OPJKBMHC_4 | 679692 | 680546 | aadK | ARO:3002627 | aadK | 100.00 | 100.00 | AL009126.1 |
| OPJKBMHC_4 | 661158 | 662360 | blt | ARO:3003006 | blt | 100.00 | 100.00 | L32599.1 |
| OPJKBMHC_3 | 631734 | 632072 | ykkC | ARO:3003063 | ykkC | 100.00 | 100.00 | AL009126.1 |
| OPJKBMHC_3 | 631417 | 631734 | ykkD | ARO:3003064 | ykkD | 99.05 | 100.00 | AL009126.1 |
| OPJKBMHC_4 | 482429 | 483598 | bmr | ARO:3003007 | bmr | 99.49 | 100.00 | M33768.1 |
| OPJKBMHC_2 | 341906 | 342826 | mphK | ARO:3004541 | mphK | 98.37 | 100.00 | CP010314.1 |
| OPJKBMHC_2 | 328580 | 330013 | lmrB | ARO:3002813 | lmrB | 99.58 | 100.00 | JYFL01000006.1 |
| OPJKBMHC_2 | 278866 | 279459 | tmrB | ARO:3003059 | tmrB | 98.48 | 100.00 | AL009126.3 |
| OPJKBMHC_5 | 251620 | 254190 | Bacillus subtilis mprF | ARO:3003324 | Bsub_mprF | 99.88 | 100.00 | AL009126.3 |
| OPJKBMHC_2 | 28898 | 30544 | vmlR | ARO:3004476 | vmlR | 99.09 | 100.18 | NC_000964.3 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| OPJKBMHC_4 | 679692 | 680546 | aadK | Streptomycin | 100.00 | 100.00 | M26879 |
| OPJKBMHC_2 | 341906 | 342826 | mph(K) | Spiramycin, Telithromycin | 98.48 | 100.00 | NC_000964 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| OPJKBMHC_4 | 679695 | 680546 | aadK | aminoglycoside 6-adenylyltransferase AadK | EXACTX | 100.00 | 100.00 | WP_003229862.1 |
| OPJKBMHC_1 | 40011 | 40529 | satA | streptothricin N-acetyltransferase SatA | EXACTX | 100.00 | 100.00 | WP_003242546.1 |
| OPJKBMHC_2 | 28898 | 30541 | vmlR | ABC-F type ribosomal protection protein VmlR | BLASTX | 99.09 | 100.00 | WP_003234144.1 |
| OPJKBMHC_2 | 341909 | 342826 | mphK | macrolide 2'-phosphotransferase MphK | BLASTX | 98.37 | 100.00 | WP_003246254.1 |
| OPJKBMHC_4 | 61047 | 63644 | rphC | rifamycin-inactivating phosphotransferase RphC | BLASTX | 81.06 | 100.00 | WP_087347987.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| OPJKBMHC_1 | 523029 | 524210 | capB | 0.0 | Capsule | Immune modulation | 99.66 | 100.00 | NP_391471 |
| OPJKBMHC_4 | 285131 | 285772 | hlyIII | 0.0 | Hemolysin III | Exotoxin | 99.53 | 100.00 | NP_390062 |
| OPJKBMHC_1 | 959172 | 959957 | dhbA | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.49 | 100.00 | NP_391080 |
| OPJKBMHC_1 | 962855 | 963793 | dhbB | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.47 | 100.00 | NP_391077 |
| OPJKBMHC_1 | 963813 | 970949 | dhbF | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.47 | 100.00 | NP_391076 |
| OPJKBMHC_4 | 10513 | 12276 | dep/capD | 0.0 | Capsule | Immune modulation | 99.15 | 100.00 | NP_389723 |
| OPJKBMHC_1 | 959983 | 961179 | dhbC | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.00 | 100.00 | NP_391079 |
| OPJKBMHC_1 | 524225 | 524674 | capC | 0.0 | Capsule | Immune modulation | 98.89 | 100.00 | NP_391470 |
| OPJKBMHC_1 | 524693 | 525835 | capA | 0.0 | Capsule | Immune modulation | 98.78 | 100.00 | NP_391469 |
| OPJKBMHC_1 | 961208 | 962827 | dhbE | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.39 | 100.00 | NP_391078 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| OPJKBMHC_6 | 28038 | 26902 | tufA | 0.0 | elongation factor Tu | 85.75 | 95.69 | AHM69299 |
| OPJKBMHC_9 | 4382 | 5251 | pdxS | 1.13e-151 | encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS | 83.79 | 98.31 | ACE61268 |
| OPJKBMHC_3 | 322899 | 322123 | CodY | 8.57e-125 | nutrient-responsive regulator | 81.08 | 100.00 | ACJ80679 |
| OPJKBMHC_1 | 676631 | 676062 | ClpP | 6.45e-99 | part of proteolytic complex | 81.05 | 97.44 | BAB94595 |
| OPJKBMHC_3 | 779245 | 778853 | spxA1 | 6.73e-70 | redox-responsive transcription factor | 83.97 | 100.00 | AAF21893 |
| OPJKBMHC_3 | 779245 | 778853 | spx | 4.02e-68 | essential gene which is a thiol/oxidative stress sensor | 80.15 | 100.00 | BAB57159 |
| OPJKBMHC_10 | 20666 | 20908 | SpoVG | 7.54e-39 | RNA-binding protein | 81.48 | 79.41 | AAT02994 |
| OPJKBMHC_2 | 76938 | 76741 | CspA | 1.88e-31 | cold shock protein | 90.91 | 100.00 | CAA62903 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| OPJKBMHC_1 | 0.0 | 1.0 | 0.0 |
| OPJKBMHC_2 | 0.0 | 1.0 | 0.0 |
| OPJKBMHC_3 | 0.0 | 1.0 | 0.0 |
| OPJKBMHC_4 | 0.0 | 1.0 | 0.0 |
| OPJKBMHC_5 | 0.0 | 1.0 | 0.0 |
| OPJKBMHC_6 | 0.0 | 1.0 | 0.0 |
| OPJKBMHC_8 | 0.0 | 1.0 | 0.0 |
| OPJKBMHC_9 | 0.0 | 1.0 | 0.0 |
| OPJKBMHC_10 | 0.0 | 1.0 | 0.0 |
| OPJKBMHC_11 | 1.0 | 0.0 | 1.0 |
| OPJKBMHC_13 | 1.0 | 0.0 | 1.0 |
| OPJKBMHC_15 | 0.0 | 1.0 | 0.0 |
| OPJKBMHC_16 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| OPJKBMHC_3 | 97162 | 120795 | False | Siphoviridae | VOG0136, VOG5221, VOG7896, VOG3390, VOG4612, VOG4705, VOG3390, VOG4626, VOG4619, VOG0801, VOG6163, VOG5910, VOG0541, VOG4586, VOG0704, VOG0539, VOG4589, VOG0204, VOG5361, VOG4553, VOG4568, VOG4556, VOG1886, VOG4581, VOG4841 |
| OPJKBMHC_3 | 661311 | 686728 | False | Myoviridae | VOG0136, VOG0753, VOG7895, VOG3390, VOG7718, VOG4862, VOG4691, VOG4550, VOG3894, VOG4885, VOG4865, VOG6163, VOG3892, VOG3891, VOG3890, VOG0749, VOG4685, VOG4773, VOG10227, VOG0796, VOG5447, VOG4861, VOG0189, VOG3490, VOG9667 |
| OPJKBMHC_4 | 216024 | 221475 | False | Unknown | VOG7483, VOG0094, VOG9030, VOG7567, VOG4570 |
| OPJKBMHC_4 | 238508 | 255648 | False | Siphoviridae | VOG9312, VOG4910, VOG3389, VOG5042, VOG9712, VOG4626, VOG3390, VOG4705, VOG8440 |
Phigaro v2.4.0 (Database: Jan. 2024 release)