Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 11 | 10 | 8 | 16.88 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| ANNLBEPN_11 | 511351 | 511689 | ykkC | ARO:3003063 | ykkC | 100.00 | 100.00 | AL009126.1 |
| ANNLBEPN_11 | 511034 | 511351 | ykkD | ARO:3003064 | ykkD | 99.05 | 100.00 | AL009126.1 |
| ANNLBEPN_3 | 376366 | 377535 | bmr | ARO:3003007 | bmr | 99.74 | 100.00 | M33768.1 |
| ANNLBEPN_7 | 345897 | 346817 | mphK | ARO:3004541 | mphK | 99.02 | 100.00 | CP010314.1 |
| ANNLBEPN_7 | 332564 | 334003 | lmrB | ARO:3002813 | lmrB | 99.16 | 100.42 | JYFL01000006.1 |
| ANNLBEPN_7 | 282858 | 283451 | tmrB | ARO:3003059 | tmrB | 97.46 | 100.00 | AL009126.3 |
| ANNLBEPN_13 | 128701 | 131271 | Bacillus subtilis mprF | ARO:3003324 | Bsub_mprF | 99.77 | 100.00 | AL009126.3 |
| ANNLBEPN_5 | 30902 | 31756 | aadK | ARO:3002627 | aadK | 98.24 | 100.00 | AL009126.1 |
| ANNLBEPN_7 | 29019 | 30665 | vmlR | ARO:3004476 | vmlR | 99.09 | 100.18 | NC_000964.3 |
| ANNLBEPN_5 | 11429 | 12631 | blt | ARO:3003006 | blt | 99.00 | 100.00 | L32599.1 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| ANNLBEPN_7 | 345897 | 346817 | mph(K) | Spiramycin, Telithromycin | 99.35 | 100.00 | NC_000964 |
| ANNLBEPN_5 | 30902 | 31756 | aadK | Streptomycin | 98.71 | 100.00 | M26879 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| ANNLBEPN_7 | 29019 | 30662 | vmlR | ABC-F type ribosomal protection protein VmlR | BLASTX | 99.09 | 100.00 | WP_003234144.1 |
| ANNLBEPN_7 | 345900 | 346817 | mphK | macrolide 2'-phosphotransferase MphK | BLASTX | 99.02 | 100.00 | WP_003246254.1 |
| ANNLBEPN_5 | 30905 | 31756 | aadK | aminoglycoside 6-adenylyltransferase AadK | BLASTX | 98.24 | 100.00 | WP_003229862.1 |
| ANNLBEPN_3 | 55494 | 58091 | rphC | rifamycin-inactivating phosphotransferase RphC | BLASTX | 80.88 | 100.00 | WP_087347987.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| ANNLBEPN_3 | 173610 | 174251 | hlyIII | 0.0 | Hemolysin III | Exotoxin | 99.69 | 100.00 | NP_390062 |
| ANNLBEPN_2 | 538618 | 539799 | capB | 0.0 | Capsule | Immune modulation | 99.66 | 100.00 | NP_391471 |
| ANNLBEPN_2 | 112728 | 113513 | dhbA | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.49 | 100.00 | NP_391080 |
| ANNLBEPN_2 | 111506 | 112702 | dhbC | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.33 | 100.00 | NP_391079 |
| ANNLBEPN_2 | 536993 | 538135 | capA | 0.0 | Capsule | Immune modulation | 99.21 | 100.00 | NP_391469 |
| ANNLBEPN_3 | 9810 | 11573 | dep/capD | 0.0 | Capsule | Immune modulation | 98.98 | 100.00 | NP_389723 |
| ANNLBEPN_2 | 108892 | 109830 | dhbB | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.72 | 100.00 | NP_391077 |
| ANNLBEPN_2 | 538154 | 538603 | capC | 0.0 | Capsule | Immune modulation | 98.67 | 100.00 | NP_391470 |
| ANNLBEPN_2 | 101736 | 108872 | dhbF | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.35 | 100.00 | NP_391076 |
| ANNLBEPN_2 | 109858 | 111477 | dhbE | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.03 | 100.00 | NP_391078 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| ANNLBEPN_8 | 27914 | 26778 | tufA | 0.0 | elongation factor Tu | 84.96 | 95.69 | AHM69299 |
| ANNLBEPN_20 | 4250 | 5119 | pdxS | 1.11e-151 | encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS | 83.79 | 98.31 | ACE61268 |
| ANNLBEPN_11 | 199149 | 198373 | CodY | 4.47e-125 | nutrient-responsive regulator | 81.08 | 100.00 | ACJ80679 |
| ANNLBEPN_2 | 385761 | 386330 | ClpP | 6.47e-99 | part of proteolytic complex | 81.05 | 97.44 | BAB94595 |
| ANNLBEPN_12 | 276202 | 276594 | spxA1 | 2.46e-70 | redox-responsive transcription factor | 83.97 | 100.00 | AAF21893 |
| ANNLBEPN_12 | 276202 | 276594 | spx | 1.47e-68 | essential gene which is a thiol/oxidative stress sensor | 80.15 | 100.00 | BAB57159 |
| ANNLBEPN_18 | 34730 | 34488 | SpoVG | 7.52e-39 | RNA-binding protein | 81.48 | 79.41 | AAT02994 |
| ANNLBEPN_7 | 78306 | 78109 | CspA | 1.91e-31 | cold shock protein | 90.91 | 100.00 | CAA62903 |
| ANNLBEPN_3 | 186213 | 186407 | CspD | 1.55e-27 | cold shock protein | 80.00 | 98.48 | CAC99957 |
| ANNLBEPN_3 | 186213 | 186410 | CspB | 3.22e-27 | cold shock protein | 80.30 | 100.00 | CAD00094 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| ANNLBEPN_1 | 0.0 | 1.0 | 0.0 |
| ANNLBEPN_2 | 0.0 | 1.0 | 0.0 |
| ANNLBEPN_3 | 0.0 | 1.0 | 0.0 |
| ANNLBEPN_5 | 0.0 | 1.0 | 0.0 |
| ANNLBEPN_7 | 0.0 | 1.0 | 0.0 |
| ANNLBEPN_8 | 0.0 | 1.0 | 0.0 |
| ANNLBEPN_9 | 0.0 | 1.0 | 0.0 |
| ANNLBEPN_10 | 0.0 | 1.0 | 0.0 |
| ANNLBEPN_11 | 0.0 | 1.0 | 0.0 |
| ANNLBEPN_12 | 0.0 | 1.0 | 0.0 |
| ANNLBEPN_13 | 0.0 | 1.0 | 0.0 |
| ANNLBEPN_14 | 0.0 | 1.0 | 0.0 |
| ANNLBEPN_16 | 0.0 | 1.0 | 0.0 |
| ANNLBEPN_18 | 0.0 | 1.0 | 0.0 |
| ANNLBEPN_19 | 0.0 | 1.0 | 0.0 |
| ANNLBEPN_20 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| ANNLBEPN_3 | 555962 | 579352 | False | Myoviridae | VOG4705, VOG7718, VOG0906, VOG4862, VOG4691, VOG4550, VOG3894, VOG4885, VOG4865, VOG6163, VOG3892, VOG3891, VOG3890, VOG10055, VOG4870, VOG4589, VOG0749, VOG4685, VOG4688, VOG4773 |
| ANNLBEPN_11 | 541220 | 547297 | False | Myoviridae | VOG0753, VOG7895, VOG3390, VOG7718, VOG4862, VOG4691, VOG4550, VOG3894 |
| ANNLBEPN_12 | 371206 | 381129 | False | Siphoviridae | VOG4602, VOG9667, VOG3490, VOG0189, VOG4861, VOG5447, VOG0796, VOG10227, VOG4773, VOG4685 |
Phigaro v2.4.0 (Database: Jan. 2024 release)