Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 12 | 10 | 8 | 17.55 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| NBAKFPBB_4 | 444078 | 445280 | blt | ARO:3003006 | blt | 98.25 | 100.00 | L32599.1 |
| NBAKFPBB_1 | 429939 | 430256 | ykkD | ARO:3003064 | ykkD | 98.10 | 100.00 | AL009126.1 |
| NBAKFPBB_1 | 429601 | 429939 | ykkC | ARO:3003063 | ykkC | 98.21 | 100.00 | AL009126.1 |
| NBAKFPBB_4 | 427633 | 428487 | aadK | ARO:3002627 | aadK | 98.24 | 100.00 | AL009126.1 |
| NBAKFPBB_3 | 332967 | 333887 | mphK | ARO:3004541 | mphK | 96.41 | 100.00 | CP010314.1 |
| NBAKFPBB_3 | 322464 | 323897 | lmrB | ARO:3002813 | lmrB | 99.16 | 100.00 | JYFL01000006.1 |
| NBAKFPBB_3 | 272750 | 273343 | tmrB | ARO:3003059 | tmrB | 97.97 | 100.00 | AL009126.3 |
| NBAKFPBB_7 | 257298 | 259868 | Bacillus subtilis mprF | ARO:3003324 | Bsub_mprF | 99.53 | 100.00 | AL009126.3 |
| NBAKFPBB_5 | 161730 | 162899 | bmr | ARO:3003007 | bmr | 98.97 | 100.00 | M33768.1 |
| NBAKFPBB_8 | 35409 | 38990 | Bacillus subtilis rpoB mutants conferring resistance to rifampin | ARO:3007073 | Bsub_rpoB_RIF | 99.92 | 100.00 | AL009126.3 |
| NBAKFPBB_3 | 13561 | 15186 | vmlR | ARO:3004476 | vmlR | 98.52 | 98.90 | NC_000964.3 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| NBAKFPBB_4 | 427633 | 428487 | aadK | Streptomycin | 98.71 | 100.00 | M26879 |
| NBAKFPBB_3 | 332967 | 333887 | mph(K) | Spiramycin, Telithromycin | 97.18 | 100.00 | NC_000964 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| NBAKFPBB_3 | 13561 | 15183 | vmlR | ABC-F type ribosomal protection protein VmlR | BLASTX | 98.52 | 98.90 | WP_003234144.1 |
| NBAKFPBB_4 | 427633 | 428484 | aadK | aminoglycoside 6-adenylyltransferase AadK | BLASTX | 98.24 | 100.00 | WP_003229862.1 |
| NBAKFPBB_3 | 332970 | 333887 | mphK | macrolide 2'-phosphotransferase MphK | BLASTX | 96.41 | 100.00 | WP_003246254.1 |
| NBAKFPBB_5 | 600176 | 602773 | rphC | rifamycin-inactivating phosphotransferase RphC | BLASTX | 80.60 | 100.00 | WP_087347987.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| NBAKFPBB_2 | 112919 | 113704 | dhbA | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.49 | 100.00 | NP_391080 |
| NBAKFPBB_2 | 518491 | 519672 | capB | 0.0 | Capsule | Immune modulation | 99.24 | 100.00 | NP_391471 |
| NBAKFPBB_5 | 653291 | 655054 | dep/capD | 0.0 | Capsule | Immune modulation | 99.15 | 100.00 | NP_389723 |
| NBAKFPBB_2 | 518027 | 518476 | capC | 0.0 | Capsule | Immune modulation | 99.11 | 100.00 | NP_391470 |
| NBAKFPBB_2 | 516866 | 518008 | capA | 0.0 | Capsule | Immune modulation | 98.95 | 100.00 | NP_391469 |
| NBAKFPBB_2 | 109082 | 110020 | dhbB | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.51 | 100.00 | NP_391077 |
| NBAKFPBB_5 | 364542 | 365183 | hlyIII | 0.0 | Hemolysin III | Exotoxin | 98.44 | 100.00 | NP_390062 |
| NBAKFPBB_2 | 101926 | 109062 | dhbF | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.28 | 100.00 | NP_391076 |
| NBAKFPBB_2 | 110048 | 111667 | dhbE | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 97.84 | 100.00 | NP_391078 |
| NBAKFPBB_2 | 111696 | 112892 | dhbC | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 96.07 | 100.00 | NP_391079 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| NBAKFPBB_8 | 28027 | 26891 | tufA | 0.0 | elongation factor Tu | 85.22 | 95.69 | AHM69299 |
| NBAKFPBB_12 | 4271 | 5140 | pdxS | 1.10e-151 | encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS | 83.79 | 98.31 | ACE61268 |
| NBAKFPBB_1 | 742263 | 743039 | CodY | 8.41e-125 | nutrient-responsive regulator | 81.08 | 100.00 | ACJ80679 |
| NBAKFPBB_2 | 376558 | 377127 | ClpP | 6.29e-99 | part of proteolytic complex | 81.05 | 97.44 | BAB94595 |
| NBAKFPBB_1 | 276092 | 276484 | spxA1 | 6.61e-70 | redox-responsive transcription factor | 83.97 | 100.00 | AAF21893 |
| NBAKFPBB_1 | 276092 | 276484 | spx | 3.95e-68 | essential gene which is a thiol/oxidative stress sensor | 80.15 | 100.00 | BAB57159 |
| NBAKFPBB_9 | 20825 | 21067 | SpoVG | 7.54e-39 | RNA-binding protein | 81.48 | 79.41 | AAT02994 |
| NBAKFPBB_3 | 69374 | 69177 | CspA | 1.83e-31 | cold shock protein | 90.91 | 100.00 | CAA62903 |
| NBAKFPBB_5 | 353313 | 353119 | CspD | 1.71e-27 | cold shock protein | 80.00 | 98.48 | CAC99957 |
| NBAKFPBB_5 | 353313 | 353116 | CspB | 3.54e-27 | cold shock protein | 80.30 | 100.00 | CAD00094 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| NBAKFPBB_1 | 0.0 | 1.0 | 0.0 |
| NBAKFPBB_2 | 0.0 | 1.0 | 0.0 |
| NBAKFPBB_3 | 0.0 | 1.0 | 0.0 |
| NBAKFPBB_4 | 0.0 | 1.0 | 0.0 |
| NBAKFPBB_5 | 0.0 | 1.0 | 0.0 |
| NBAKFPBB_7 | 0.0 | 1.0 | 0.0 |
| NBAKFPBB_8 | 0.0 | 1.0 | 0.0 |
| NBAKFPBB_9 | 0.0 | 1.0 | 0.0 |
| NBAKFPBB_10 | 0.0 | 1.0 | 0.0 |
| NBAKFPBB_11 | 0.0 | 1.0 | 0.0 |
| NBAKFPBB_12 | 0.0 | 1.0 | 0.0 |
| NBAKFPBB_13 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| NBAKFPBB_1 | 373734 | 399781 | False | Myoviridae | VOG4602, VOG9667, VOG3490, VOG0189, VOG4861, VOG5447, VOG0796, VOG10227, VOG4773, VOG4685, VOG0749, VOG3890, VOG3891, VOG3892, VOG6163, VOG4865, VOG4885, VOG3894, VOG4550, VOG4691, VOG4862, VOG7718, VOG4705 |
| NBAKFPBB_5 | 414415 | 425285 | False | Siphoviridae | VOG5534, VOG6940, VOG9713, VOG9219, VOG5495, VOG6036, VOG9867, VOG7134, VOG10940, VOG5721 |
Phigaro v2.4.0 (Database: Jan. 2024 release)