Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 15 | 10 | 9 | 20.15 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| KFOAHEIJ_2 | 869890 | 870228 | ykkC | ARO:3003063 | ykkC | 100.00 | 100.00 | AL009126.1 |
| KFOAHEIJ_2 | 869573 | 869890 | ykkD | ARO:3003064 | ykkD | 100.00 | 100.00 | AL009126.1 |
| KFOAHEIJ_5 | 678436 | 679605 | bmr | ARO:3003007 | bmr | 99.74 | 100.00 | M33768.1 |
| KFOAHEIJ_5 | 456085 | 457287 | blt | ARO:3003006 | blt | 100.00 | 100.00 | L32599.1 |
| KFOAHEIJ_5 | 437724 | 438578 | aadK | ARO:3002627 | aadK | 100.00 | 100.00 | AL009126.1 |
| KFOAHEIJ_3 | 280327 | 281247 | mphK | ARO:3004541 | mphK | 100.00 | 100.00 | CP010314.1 |
| KFOAHEIJ_7 | 276815 | 279385 | Bacillus subtilis mprF | ARO:3003324 | Bsub_mprF | 100.00 | 100.00 | AL009126.3 |
| KFOAHEIJ_3 | 266993 | 268432 | lmrB | ARO:3002813 | lmrB | 99.79 | 100.42 | JYFL01000006.1 |
| KFOAHEIJ_3 | 217336 | 217929 | tmrB | ARO:3003059 | tmrB | 100.00 | 100.00 | AL009126.3 |
| KFOAHEIJ_12 | 47376 | 49019 | vmlR | ARO:3004476 | vmlR | 100.00 | 100.00 | NC_000964.3 |
| KFOAHEIJ_4 | 3435 | 4205 | ANT(4')-Ia | ARO:3002623 | ANT(4')-Ia | 99.21 | 101.19 | K02551.1 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| KFOAHEIJ_4 | 3444 | 4205 | aadD | Amikacin, Tobramycin | 100.00 | 100.00 | M19465 |
| KFOAHEIJ_5 | 437724 | 438578 | aadK | Streptomycin | 100.00 | 100.00 | M26879 |
| KFOAHEIJ_3 | 280327 | 281247 | mph(K) | Spiramycin, Telithromycin | 100.00 | 100.00 | NC_000964 |
| KFOAHEIJ_1 | 1008973 | 1010349 | tet(L) | Doxycycline, Tetracycline, Doxycycline, Tetracycline, Minocycline, Tigecycline | 100.00 | 100.00 | X08034 |
| KFOAHEIJ_4 | 4428 | 4804 | bleO | Bleomycin | 100.0 | 94.49 | AF051917 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| KFOAHEIJ_4 | 3435 | 4202 | aadD1 | aminoglycoside O-nucleotidyltransferase ANT(4')-Ia | EXACTX | 100.00 | 100.00 | WP_001795128.1 |
| KFOAHEIJ_5 | 437724 | 438575 | aadK | aminoglycoside 6-adenylyltransferase AadK | EXACTX | 100.00 | 100.00 | WP_003229862.1 |
| KFOAHEIJ_3 | 280330 | 281247 | mphK | macrolide 2'-phosphotransferase MphK | EXACTX | 100.00 | 100.00 | WP_003246254.1 |
| KFOAHEIJ_1 | 1006455 | 1006973 | satA | streptothricin N-acetyltransferase SatA | EXACTX | 100.00 | 100.00 | WP_003242546.1 |
| KFOAHEIJ_1 | 1008976 | 1010349 | tet(L) | tetracycline efflux MFS transporter Tet(L) | EXACTX | 100.00 | 100.00 | WP_003242953.1 |
| KFOAHEIJ_12 | 47379 | 49019 | vmlR | ABC-F type ribosomal protection protein VmlR | EXACTX | 100.00 | 100.00 | WP_003234144.1 |
| KFOAHEIJ_4 | 4422 | 4802 | bleO | bleomycin binding protein | BLASTX | 100.00 | 94.78 | WP_001242578.1 |
| KFOAHEIJ_2 | 192816 | 195410 | rphC | rifamycin-inactivating phosphotransferase RphC | BLASTX | 81.15 | 99.88 | WP_087347987.1 |
| KFOAHEIJ_4 | 3 | 275 | bleO | bleomycin binding protein | PARTIAL_CONTIG_ENDX | 98.90 | 67.91 | WP_001242578.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| KFOAHEIJ_1 | 519113 | 520255 | capA | 0.0 | Capsule | Immune modulation | 100.00 | 100.00 | NP_391469 |
| KFOAHEIJ_1 | 520738 | 521919 | capB | 0.0 | Capsule | Immune modulation | 100.00 | 100.00 | NP_391471 |
| KFOAHEIJ_1 | 520274 | 520723 | capC | 0.0 | Capsule | Immune modulation | 100.00 | 100.00 | NP_391470 |
| KFOAHEIJ_2 | 240305 | 242068 | dep/capD | 0.0 | Capsule | Immune modulation | 100.00 | 100.00 | NP_389723 |
| KFOAHEIJ_1 | 112803 | 113588 | dhbA | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 100.00 | 100.00 | NP_391080 |
| KFOAHEIJ_1 | 108967 | 109905 | dhbB | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 100.00 | 100.00 | NP_391077 |
| KFOAHEIJ_1 | 111581 | 112777 | dhbC | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 100.00 | 100.00 | NP_391079 |
| KFOAHEIJ_1 | 109933 | 111552 | dhbE | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 100.00 | 100.00 | NP_391078 |
| KFOAHEIJ_1 | 101811 | 108947 | dhbF | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 100.00 | 100.00 | NP_391076 |
| KFOAHEIJ_5 | 878316 | 878957 | hlyIII | 0.0 | Hemolysin III | Exotoxin | 100.00 | 100.00 | NP_390062 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| KFOAHEIJ_8 | 31693 | 32829 | tufA | 0.0 | elongation factor Tu | 84.96 | 95.69 | AHM69299 |
| KFOAHEIJ_10 | 11347 | 10478 | pdxS | 1.15e-151 | encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS | 83.79 | 98.31 | ACE61268 |
| KFOAHEIJ_2 | 556625 | 555849 | CodY | 7.94e-125 | nutrient-responsive regulator | 81.08 | 100.00 | ACJ80679 |
| KFOAHEIJ_1 | 367526 | 368095 | ClpP | 6.29e-99 | part of proteolytic complex | 81.05 | 97.44 | BAB94595 |
| KFOAHEIJ_6 | 40219 | 39827 | spxA1 | 7.61e-70 | redox-responsive transcription factor | 83.21 | 100.00 | AAF21893 |
| KFOAHEIJ_9 | 34693 | 34451 | SpoVG | 2.74e-38 | RNA-binding protein | 80.25 | 79.41 | AAT02994 |
| KFOAHEIJ_12 | 1904 | 2101 | CspA | 3.38e-32 | cold shock protein | 90.91 | 100.00 | CAA62903 |
| KFOAHEIJ_5 | 866354 | 866160 | CspD | 5.70e-28 | cold shock protein | 81.54 | 98.48 | CAC99957 |
| KFOAHEIJ_6 | 283451 | 283645 | CspB | 2.91e-27 | cold shock protein | 83.08 | 98.48 | CAD00094 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| KFOAHEIJ_4 | 2271 | 3275 | rep22 | 1005 / 1005 | 100.00 | AF503772 |
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| KFOAHEIJ_1 | 0.0 | 1.0 | 0.0 |
| KFOAHEIJ_2 | 0.0 | 1.0 | 0.0 |
| KFOAHEIJ_3 | 0.0 | 1.0 | 0.0 |
| KFOAHEIJ_4 | 1.0 | 0.0 | 1.0 |
| KFOAHEIJ_5 | 0.0 | 1.0 | 0.0 |
| KFOAHEIJ_6 | 0.0 | 1.0 | 0.0 |
| KFOAHEIJ_7 | 0.0 | 1.0 | 0.0 |
| KFOAHEIJ_8 | 0.0 | 1.0 | 0.0 |
| KFOAHEIJ_9 | 0.0 | 1.0 | 0.0 |
| KFOAHEIJ_10 | 0.0 | 1.0 | 0.0 |
| KFOAHEIJ_12 | 0.0 | 1.0 | 0.0 |
| KFOAHEIJ_15 | 1.0 | 0.0 | 1.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| KFOAHEIJ_2 | 900067 | 926175 | False | Myoviridae | VOG4705, VOG7718, VOG4862, VOG4691, VOG4550, VOG3894, VOG4885, VOG4865, VOG6163, VOG3892, VOG3891, VOG3890, VOG0749, VOG4685, VOG4773, VOG10227, VOG0796, VOG5447, VOG4861, VOG0189, VOG3490, VOG9667 |
| KFOAHEIJ_5 | 476838 | 507215 | False | Myoviridae | VOG4600, VOG1309, VOG11060, VOG0189, VOG4693, VOG9587, VOG4673, VOG0796, VOG10227, VOG4773, VOG4688, VOG4685, VOG0749, VOG4589, VOG4870, VOG10055, VOG3890, VOG3891, VOG3892, VOG6163, VOG4865, VOG4885, VOG3894, VOG4550, VOG4691, VOG4862, VOG7718 |
Phigaro v2.4.0 (Database: Jan. 2024 release)