Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 4 | 2 | 6 | 7.48 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| NNAPIBNL_8 | 159701 | 160747 | clbA | ARO:3002814 | clbA | 92.77 | 99.71 | CP006845.1 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| NNAPIBNL_8 | 159701 | 160744 | cfr(B) | Chloramphenicol, Florfenicol, Clindamycin, Lincomycin, Linezolid, Dalfopristin, Pristinamycin IIA, Virginiamycin M, Tiamulin | 88.90 | 99.33 | KR610408 |
| NNAPIBNL_31 | 57960 | 59336 | tet(L) | Doxycycline, Tetracycline, Doxycycline, Tetracycline, Minocycline, Tigecycline | 87.07 | 100.00 | X08034 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| NNAPIBNL_8 | 159701 | 160744 | clbA | 23S rRNA (adenine(2503)-C(8))-methyltransferase ClbA | BLASTX | 92.24 | 99.71 | WP_012116915.1 |
| NNAPIBNL_31 | 57960 | 59330 | tet(L) | tetracycline efflux MFS transporter Tet(L) | BLASTX | 86.43 | 99.78 | WP_003242953.1 |
| NNAPIBNL_8 | 157432 | 157950 | satA | streptothricin N-acetyltransferase SatA | BLASTX | 82.66 | 100.00 | WP_003242546.1 |
| NNAPIBNL_46 | 53995 | 56592 | rphC | rifamycin-inactivating phosphotransferase RphC | INTERNAL_STOP | 80.94 | 100.00 | WP_087347987.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| NNAPIBNL_18 | 35054 | 36235 | capB | 0.0 | Capsule | Immune modulation | 81.79 | 99.66 | NP_391471 |
| NNAPIBNL_18 | 36250 | 36699 | capC | 1.09e-114 | Capsule | Immune modulation | 83.59 | 99.78 | NP_391470 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| NNAPIBNL_20 | 31913 | 33049 | tufA | 0.0 | elongation factor Tu | 86.54 | 95.69 | AHM69299 |
| NNAPIBNL_42 | 12390 | 11521 | pdxS | 6.31e-153 | encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS | 84.83 | 98.31 | ACE61268 |
| NNAPIBNL_72 | 5985 | 5209 | CodY | 5.34e-127 | nutrient-responsive regulator | 81.08 | 100.00 | ACJ80679 |
| NNAPIBNL_7 | 42031 | 42423 | spxA1 | 1.27e-70 | redox-responsive transcription factor | 83.21 | 100.00 | AAF21893 |
| NNAPIBNL_8 | 143215 | 143412 | CspA | 3.14e-32 | cold shock protein | 92.42 | 100.00 | CAA62903 |
| NNAPIBNL_16 | 40860 | 40666 | CspD | 3.46e-28 | cold shock protein | 80.00 | 98.48 | CAC99957 |
| NNAPIBNL_16 | 40860 | 40663 | CspB | 7.18e-28 | cold shock protein | 80.30 | 100.00 | CAD00094 |
| NNAPIBNL_8 | 143215 | 143409 | CspR | 1.56e-26 | cold shock protein | 80.00 | 98.48 | AAO82613 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| NNAPIBNL_1 | 0.0 | 1.0 | 0.0 |
| NNAPIBNL_2 | 0.0 | 1.0 | 0.0 |
| NNAPIBNL_3 | 0.0 | 1.0 | 0.0 |
| NNAPIBNL_4 | 0.0 | 1.0 | 0.0 |
| NNAPIBNL_5 | 0.0 | 1.0 | 0.0 |
| NNAPIBNL_6 | 0.0 | 1.0 | 0.0 |
| NNAPIBNL_7 | 0.0 | 1.0 | 0.0 |
| NNAPIBNL_8 | 0.0 | 1.0 | 0.0 |
| NNAPIBNL_9 | 0.0 | 1.0 | 0.0 |
| NNAPIBNL_10 | 0.0 | 1.0 | 0.0 |
| NNAPIBNL_11 | 0.0 | 1.0 | 0.0 |
| NNAPIBNL_12 | 0.0 | 1.0 | 0.0 |
| NNAPIBNL_13 | 0.0 | 1.0 | 0.0 |
| NNAPIBNL_14 | 0.0 | 1.0 | 0.0 |
| NNAPIBNL_15 | 0.0 | 1.0 | 0.0 |
| NNAPIBNL_16 | 0.0 | 1.0 | 0.0 |
| NNAPIBNL_17 | 0.0 | 1.0 | 0.0 |
| NNAPIBNL_18 | 0.0 | 1.0 | 0.0 |
| NNAPIBNL_19 | 0.0 | 1.0 | 0.0 |
| NNAPIBNL_20 | 0.0 | 1.0 | 0.0 |
| NNAPIBNL_21 | 0.0 | 1.0 | 0.0 |
| NNAPIBNL_22 | 0.0 | 1.0 | 0.0 |
| NNAPIBNL_23 | 0.0 | 1.0 | 0.0 |
| NNAPIBNL_24 | 0.0 | 1.0 | 0.0 |
| NNAPIBNL_25 | 0.0 | 1.0 | 0.0 |
| NNAPIBNL_26 | 0.0 | 1.0 | 0.0 |
| NNAPIBNL_27 | 0.0 | 1.0 | 0.0 |
| NNAPIBNL_28 | 0.0 | 1.0 | 0.0 |
| NNAPIBNL_29 | 0.0 | 1.0 | 0.0 |
| NNAPIBNL_30 | 0.0 | 1.0 | 0.0 |
| NNAPIBNL_31 | 0.0 | 1.0 | 0.0 |
| NNAPIBNL_32 | 0.0 | 1.0 | 0.0 |
| NNAPIBNL_33 | 0.0 | 1.0 | 0.0 |
| NNAPIBNL_34 | 0.0 | 1.0 | 0.0 |
| NNAPIBNL_35 | 0.0 | 1.0 | 0.0 |
| NNAPIBNL_36 | 0.0 | 1.0 | 0.0 |
| NNAPIBNL_37 | 0.0 | 1.0 | 0.0 |
| NNAPIBNL_38 | 0.0 | 1.0 | 0.0 |
| NNAPIBNL_39 | 0.0 | 1.0 | 0.0 |
| NNAPIBNL_40 | 0.0 | 1.0 | 0.0 |
| NNAPIBNL_41 | 0.0 | 1.0 | 0.0 |
| NNAPIBNL_42 | 0.0 | 1.0 | 0.0 |
| NNAPIBNL_43 | 0.0 | 1.0 | 0.0 |
| NNAPIBNL_44 | 0.0 | 1.0 | 0.0 |
| NNAPIBNL_45 | 0.0 | 1.0 | 0.0 |
| NNAPIBNL_46 | 0.0 | 1.0 | 0.0 |
| NNAPIBNL_47 | 0.0 | 1.0 | 0.0 |
| NNAPIBNL_48 | 0.0 | 1.0 | 0.0 |
| NNAPIBNL_49 | 0.0 | 1.0 | 0.0 |
| NNAPIBNL_50 | 0.0 | 1.0 | 0.0 |
| NNAPIBNL_51 | 0.0 | 1.0 | 0.0 |
| NNAPIBNL_52 | 0.0 | 1.0 | 0.0 |
| NNAPIBNL_54 | 0.0 | 1.0 | 0.0 |
| NNAPIBNL_55 | 0.0 | 1.0 | 0.0 |
| NNAPIBNL_56 | 1.0 | 0.0 | 1.0 |
| NNAPIBNL_58 | 0.0 | 1.0 | 0.0 |
| NNAPIBNL_59 | 0.0 | 1.0 | 0.0 |
| NNAPIBNL_60 | 0.0 | 1.0 | 0.0 |
| NNAPIBNL_61 | 0.0 | 1.0 | 0.0 |
| NNAPIBNL_62 | 0.0 | 1.0 | 0.0 |
| NNAPIBNL_63 | 0.0 | 1.0 | 0.0 |
| NNAPIBNL_65 | 0.0 | 1.0 | 0.0 |
| NNAPIBNL_66 | 0.0 | 1.0 | 0.0 |
| NNAPIBNL_69 | 0.0 | 1.0 | 0.0 |
| NNAPIBNL_70 | 0.0 | 1.0 | 0.0 |
| NNAPIBNL_71 | 0.0 | 1.0 | 0.0 |
| NNAPIBNL_72 | 0.0 | 1.0 | 0.0 |
| NNAPIBNL_74 | 0.0 | 1.0 | 0.0 |
| NNAPIBNL_75 | 0.0 | 1.0 | 0.0 |
| NNAPIBNL_76 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| NNAPIBNL_10 | 2 | 29886 | False | Siphoviridae | VOG4660, VOG4619, VOG0801, VOG6163, VOG0541, VOG4586, VOG0704, VOG0539, VOG4589, VOG0204, VOG5361, VOG4553, VOG4568, VOG4556, VOG5051, VOG4544, VOG4581, VOG0198, VOG4548, VOG0327, VOG4609, VOG10500, VOG0026, VOG1565, VOG0021, VOG1047, VOG7438 |
| NNAPIBNL_11 | 20811 | 49878 | False | Siphoviridae | VOG4666, VOG4574, VOG0136, VOG0753, VOG5535, VOG3390, VOG4599, VOG4605, VOG4545, VOG0725, VOG0799, VOG0724, VOG5660, VOG0816, VOG4623, VOG5872, VOG2621, VOG1329, VOG4555, VOG6778, VOG4564, VOG0720, VOG10227, VOG0796, VOG4681, VOG4972, VOG5447 |
| NNAPIBNL_11 | 52250 | 66196 | False | Siphoviridae | VOG0102, VOG6581, VOG0397, VOG8569, VOG4658, VOG1336, VOG0536, VOG0044, VOG0189, VOG9093, VOG1309, VOG4600, VOG4600, VOG4552, VOG0650, VOG11075, VOG9667, VOG8483 |
| NNAPIBNL_12 | 49106 | 67180 | False | Siphoviridae | VOG0181, VOG4552, VOG3547, VOG4566, VOG9600, VOG0025, VOG1884, VOG5969, VOG0425, VOG5447, VOG0376, VOG2545, VOG4841, VOG4581, VOG4544, VOG4556, VOG4573, VOG4553, VOG0204, VOG4589, VOG0207 |
| NNAPIBNL_15 | 36155 | 63236 | False | Myoviridae | VOG0136, VOG0753, VOG4705, VOG3390, VOG7718, VOG4862, VOG4691, VOG4550, VOG3894, VOG4885, VOG4865, VOG6163, VOG3892, VOG3891, VOG3890, VOG10055, VOG0749, VOG4685, VOG4773, VOG10227, VOG0796, VOG5447, VOG4861, VOG0189 |
| NNAPIBNL_17 | 15953 | 38272 | False | Siphoviridae | VOG4972, VOG0641, VOG4705, VOG3390, VOG4612, VOG4660, VOG4619, VOG0801, VOG6163, VOG5910, VOG0541, VOG4586, VOG0704, VOG0539, VOG4589, VOG0204, VOG4553, VOG4568, VOG4556, VOG5051, VOG4544, VOG4581, VOG0531, VOG6052 |
| NNAPIBNL_40 | 8692 | 23825 | False | Siphoviridae | VOG11074, VOG2565, VOG0724, VOG5361, VOG3676, VOG4555, VOG0692, VOG2613, VOG3699, VOG5023, VOG0275, VOG1183, VOG5327, VOG6495, VOG7742, VOG7541 |
| NNAPIBNL_40 | 43799 | 47057 | False | Unknown | VOG10564, VOG10937 |
Phigaro v2.4.0 (Database: Jan. 2024 release)