Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 10 | 10 | 9 | 16.76 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| CBGOONLN_2 | 107165 | 108604 | lmrB | ARO:3002813 | lmrB | 99.58 | 100.42 | JYFL01000006.1 |
| CBGOONLN_2 | 94350 | 95270 | mphK | ARO:3004541 | mphK | 98.68 | 100.00 | CP010314.1 |
| CBGOONLN_17 | 60971 | 62173 | blt | ARO:3003006 | blt | 98.00 | 100.00 | L32599.1 |
| CBGOONLN_7 | 59472 | 62042 | Bacillus subtilis mprF | ARO:3003324 | Bsub_mprF | 99.53 | 100.00 | AL009126.3 |
| CBGOONLN_17 | 44222 | 45076 | aadK | ARO:3002627 | aadK | 98.59 | 100.00 | AL009126.1 |
| CBGOONLN_21 | 40604 | 40942 | ykkC | ARO:3003063 | ykkC | 98.21 | 100.00 | AL009126.1 |
| CBGOONLN_21 | 40287 | 40604 | ykkD | ARO:3003064 | ykkD | 98.10 | 100.00 | AL009126.1 |
| CBGOONLN_8 | 18944 | 19537 | tmrB | ARO:3003059 | tmrB | 99.49 | 100.00 | AL009126.3 |
| CBGOONLN_46 | 5623 | 7269 | vmlR | ARO:3004476 | vmlR | 98.36 | 100.18 | NC_000964.3 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| CBGOONLN_17 | 44222 | 45076 | aadK | Streptomycin | 98.83 | 100.00 | M26879 |
| CBGOONLN_2 | 94350 | 95270 | mph(K) | Spiramycin, Telithromycin | 98.05 | 100.00 | NC_000964 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| CBGOONLN_2 | 94350 | 95258 | mphK | macrolide 2'-phosphotransferase MphK | BLASTX | 98.68 | 99.02 | WP_003246254.1 |
| CBGOONLN_17 | 44222 | 45073 | aadK | aminoglycoside 6-adenylyltransferase AadK | BLASTX | 98.59 | 100.00 | WP_003229862.1 |
| CBGOONLN_46 | 5626 | 7269 | vmlR | ABC-F type ribosomal protection protein VmlR | BLASTX | 98.36 | 100.00 | WP_003234144.1 |
| CBGOONLN_35 | 51033 | 51551 | satA | streptothricin N-acetyltransferase SatA | BLASTX | 90.75 | 100.00 | WP_003242546.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| CBGOONLN_25 | 253472 | 254653 | capB | 0.0 | Capsule | Immune modulation | 99.66 | 100.00 | NP_391471 |
| CBGOONLN_27 | 112754 | 113539 | dhbA | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.49 | 100.00 | NP_391080 |
| CBGOONLN_25 | 255136 | 256278 | capA | 0.0 | Capsule | Immune modulation | 99.30 | 100.00 | NP_391469 |
| CBGOONLN_27 | 101762 | 108898 | dhbF | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.30 | 100.00 | NP_391076 |
| CBGOONLN_47 | 1676 | 3439 | dep/capD | 0.0 | Capsule | Immune modulation | 99.04 | 100.00 | NP_389723 |
| CBGOONLN_27 | 108918 | 109856 | dhbB | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.04 | 100.00 | NP_391077 |
| CBGOONLN_27 | 111532 | 112728 | dhbC | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.00 | 100.00 | NP_391079 |
| CBGOONLN_13 | 143882 | 144523 | hlyIII | 0.0 | Hemolysin III | Exotoxin | 98.91 | 100.00 | NP_390062 |
| CBGOONLN_25 | 254668 | 255117 | capC | 0.0 | Capsule | Immune modulation | 98.89 | 100.00 | NP_391470 |
| CBGOONLN_27 | 109884 | 111503 | dhbE | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 96.73 | 100.00 | NP_391078 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| CBGOONLN_29 | 31999 | 33135 | tufA | 0.0 | elongation factor Tu | 84.96 | 95.69 | AHM69299 |
| CBGOONLN_58 | 11735 | 10866 | pdxS | 2.01e-151 | encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS | 83.45 | 98.31 | ACE61268 |
| CBGOONLN_15 | 151536 | 152312 | CodY | 1.99e-125 | nutrient-responsive regulator | 81.08 | 100.00 | ACJ80679 |
| CBGOONLN_14 | 45230 | 44661 | ClpP | 2.90e-100 | part of proteolytic complex | 81.05 | 97.44 | BAB94595 |
| CBGOONLN_10 | 41580 | 41188 | spxA1 | 4.12e-71 | redox-responsive transcription factor | 83.97 | 100.00 | AAF21893 |
| CBGOONLN_10 | 41580 | 41188 | spx | 2.46e-69 | essential gene which is a thiol/oxidative stress sensor | 80.15 | 100.00 | BAB57159 |
| CBGOONLN_39 | 20630 | 20872 | SpoVG | 7.53e-39 | RNA-binding protein | 81.48 | 79.41 | AAT02994 |
| CBGOONLN_4 | 40466 | 40269 | CspA | 2.35e-32 | cold shock protein | 90.91 | 100.00 | CAA62903 |
| CBGOONLN_13 | 132652 | 132458 | CspD | 1.09e-28 | cold shock protein | 81.54 | 98.48 | CAC99957 |
| CBGOONLN_1 | 32041 | 31847 | CspB | 4.10e-28 | cold shock protein | 83.08 | 98.48 | CAD00094 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| CBGOONLN_55 | 4377 | 4544 | Col(SD853) | 168 / 194 | 86.90 | NC015392 |
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| CBGOONLN_1 | 0.0 | 1.0 | 0.0 |
| CBGOONLN_2 | 0.0 | 1.0 | 0.0 |
| CBGOONLN_3 | 0.0 | 1.0 | 0.0 |
| CBGOONLN_4 | 0.0 | 1.0 | 0.0 |
| CBGOONLN_5 | 0.0 | 1.0 | 0.0 |
| CBGOONLN_6 | 0.0 | 1.0 | 0.0 |
| CBGOONLN_7 | 0.0 | 1.0 | 0.0 |
| CBGOONLN_8 | 0.0 | 1.0 | 0.0 |
| CBGOONLN_9 | 0.0 | 1.0 | 0.0 |
| CBGOONLN_10 | 0.0 | 1.0 | 0.0 |
| CBGOONLN_11 | 0.0 | 1.0 | 0.0 |
| CBGOONLN_12 | 0.0 | 1.0 | 0.0 |
| CBGOONLN_13 | 0.0 | 1.0 | 0.0 |
| CBGOONLN_14 | 0.0 | 1.0 | 0.0 |
| CBGOONLN_15 | 0.0 | 1.0 | 0.0 |
| CBGOONLN_16 | 0.0 | 1.0 | 0.0 |
| CBGOONLN_17 | 0.0 | 1.0 | 0.0 |
| CBGOONLN_18 | 0.0 | 1.0 | 0.0 |
| CBGOONLN_19 | 0.0 | 1.0 | 0.0 |
| CBGOONLN_20 | 0.0 | 1.0 | 0.0 |
| CBGOONLN_21 | 0.0 | 1.0 | 0.0 |
| CBGOONLN_22 | 0.0 | 1.0 | 0.0 |
| CBGOONLN_23 | 0.0 | 1.0 | 0.0 |
| CBGOONLN_24 | 0.0 | 1.0 | 0.0 |
| CBGOONLN_25 | 0.0 | 1.0 | 0.0 |
| CBGOONLN_26 | 0.0 | 1.0 | 0.0 |
| CBGOONLN_27 | 0.0 | 1.0 | 0.0 |
| CBGOONLN_28 | 0.0 | 1.0 | 0.0 |
| CBGOONLN_29 | 0.0 | 1.0 | 0.0 |
| CBGOONLN_31 | 0.0 | 1.0 | 0.0 |
| CBGOONLN_32 | 1.0 | 0.0 | 1.0 |
| CBGOONLN_33 | 0.0 | 1.0 | 0.0 |
| CBGOONLN_34 | 0.0 | 1.0 | 0.0 |
| CBGOONLN_35 | 0.0 | 1.0 | 0.0 |
| CBGOONLN_36 | 0.0 | 1.0 | 0.0 |
| CBGOONLN_37 | 0.0 | 1.0 | 0.0 |
| CBGOONLN_38 | 1.0 | 0.0 | 1.0 |
| CBGOONLN_39 | 0.0 | 1.0 | 0.0 |
| CBGOONLN_40 | 0.0 | 1.0 | 0.0 |
| CBGOONLN_41 | 0.0 | 1.0 | 0.0 |
| CBGOONLN_42 | 0.0 | 1.0 | 0.0 |
| CBGOONLN_43 | 0.0 | 1.0 | 0.0 |
| CBGOONLN_44 | 0.0 | 1.0 | 0.0 |
| CBGOONLN_45 | 0.0 | 1.0 | 0.0 |
| CBGOONLN_46 | 0.0 | 1.0 | 0.0 |
| CBGOONLN_47 | 0.0 | 1.0 | 0.0 |
| CBGOONLN_48 | 1.0 | 0.0 | 1.0 |
| CBGOONLN_49 | 0.0 | 1.0 | 0.0 |
| CBGOONLN_50 | 0.0 | 1.0 | 0.0 |
| CBGOONLN_51 | 0.0 | 1.0 | 0.0 |
| CBGOONLN_52 | 0.0 | 1.0 | 0.0 |
| CBGOONLN_53 | 1.0 | 0.0 | 1.0 |
| CBGOONLN_54 | 0.0 | 1.0 | 0.0 |
| CBGOONLN_55 | 1.0 | 0.0 | 1.0 |
| CBGOONLN_56 | 0.0 | 1.0 | 0.0 |
| CBGOONLN_57 | 0.0 | 1.0 | 0.0 |
| CBGOONLN_58 | 0.0 | 1.0 | 0.0 |
| CBGOONLN_59 | 1.0 | 0.0 | 1.0 |
| CBGOONLN_60 | 0.0 | 1.0 | 0.0 |
| CBGOONLN_61 | 0.0 | 1.0 | 0.0 |
| CBGOONLN_62 | 0.0 | 1.0 | 0.0 |
| CBGOONLN_63 | 1.0 | 0.0 | 1.0 |
| CBGOONLN_64 | 0.0 | 1.0 | 0.0 |
| CBGOONLN_65 | 0.0 | 1.0 | 0.0 |
| CBGOONLN_66 | 0.0 | 1.0 | 0.0 |
| CBGOONLN_67 | 1.0 | 0.0 | 1.0 |
| CBGOONLN_68 | 0.0 | 1.0 | 0.0 |
| CBGOONLN_69 | 0.0 | 1.0 | 0.0 |
| CBGOONLN_70 | 1.0 | 0.0 | 1.0 |
| CBGOONLN_71 | 0.0 | 1.0 | 0.0 |
| CBGOONLN_72 | 1.0 | 0.0 | 1.0 |
| CBGOONLN_73 | 1.0 | 0.0 | 1.0 |
| CBGOONLN_74 | 0.0 | 1.0 | 0.0 |
| CBGOONLN_75 | 0.0 | 1.0 | 0.0 |
| CBGOONLN_76 | 0.0 | 1.0 | 0.0 |
| CBGOONLN_77 | 0.0 | 1.0 | 0.0 |
| CBGOONLN_79 | 0.0 | 1.0 | 0.0 |
| CBGOONLN_80 | 0.0 | 1.0 | 0.0 |
| CBGOONLN_81 | 0.0 | 1.0 | 0.0 |
| CBGOONLN_88 | 1.0 | 0.0 | 1.0 |
| CBGOONLN_89 | 0.0 | 1.0 | 0.0 |
| CBGOONLN_92 | 0.0 | 1.0 | 0.0 |
| CBGOONLN_94 | 0.0 | 1.0 | 0.0 |
| CBGOONLN_96 | 1.0 | 0.0 | 1.0 |
| CBGOONLN_97 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| CBGOONLN_21 | 70406 | 97089 | False | Myoviridae | VOG0753, VOG4705, VOG7718, VOG4862, VOG4691, VOG4550, VOG3894, VOG4885, VOG4865, VOG6163, VOG3892, VOG3891, VOG3890, VOG0749, VOG4685, VOG4773, VOG10227, VOG0796, VOG5447, VOG4843, VOG0189, VOG3490 |
| CBGOONLN_26 | 1 | 26619 | False | Siphoviridae | VOG4626, VOG4619, VOG0801, VOG6163, VOG5910, VOG0541, VOG4586, VOG0704, VOG0539, VOG4589, VOG0204, VOG5361, VOG4553, VOG4568, VOG4556, VOG3644, VOG4544, VOG4581, VOG7403, VOG4841, VOG0198, VOG7372, VOG2752, VOG4606, VOG1227, VOG1936, VOG0535, VOG0650 |
| CBGOONLN_31 | 4689 | 15219 | True | Siphoviridae | VOG6587, VOG0189, VOG4566, VOG7581, VOG9667, VOG9667, VOG1563, VOG0275, VOG0275 |
Phigaro v2.4.0 (Database: Jan. 2024 release)