Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 10 | 10 | 9 | 16.76 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| HEIKHJEJ_2 | 62306 | 62623 | ykkD | ARO:3003064 | ykkD | 98.10 | 100.00 | AL009126.1 |
| HEIKHJEJ_2 | 61968 | 62306 | ykkC | ARO:3003063 | ykkC | 98.21 | 100.00 | AL009126.1 |
| HEIKHJEJ_24 | 61162 | 62364 | blt | ARO:3003006 | blt | 98.00 | 100.00 | L32599.1 |
| HEIKHJEJ_24 | 44413 | 45267 | aadK | ARO:3002627 | aadK | 98.59 | 100.00 | AL009126.1 |
| HEIKHJEJ_28 | 35175 | 36614 | lmrB | ARO:3002813 | lmrB | 99.58 | 100.42 | JYFL01000006.1 |
| HEIKHJEJ_52 | 27212 | 29782 | Bacillus subtilis mprF | ARO:3003324 | Bsub_mprF | 99.53 | 100.00 | AL009126.3 |
| HEIKHJEJ_28 | 22360 | 23280 | mphK | ARO:3004541 | mphK | 98.68 | 100.00 | CP010314.1 |
| HEIKHJEJ_35 | 5781 | 7427 | vmlR | ARO:3004476 | vmlR | 98.36 | 100.18 | NC_000964.3 |
| HEIKHJEJ_73 | 871 | 1464 | tmrB | ARO:3003059 | tmrB | 99.49 | 100.00 | AL009126.3 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| HEIKHJEJ_24 | 44413 | 45267 | aadK | Streptomycin | 98.83 | 100.00 | M26879 |
| HEIKHJEJ_28 | 22360 | 23280 | mph(K) | Spiramycin, Telithromycin | 98.05 | 100.00 | NC_000964 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| HEIKHJEJ_28 | 22360 | 23268 | mphK | macrolide 2'-phosphotransferase MphK | BLASTX | 98.68 | 99.02 | WP_003246254.1 |
| HEIKHJEJ_24 | 44413 | 45264 | aadK | aminoglycoside 6-adenylyltransferase AadK | BLASTX | 98.59 | 100.00 | WP_003229862.1 |
| HEIKHJEJ_35 | 5784 | 7427 | vmlR | ABC-F type ribosomal protection protein VmlR | BLASTX | 98.36 | 100.00 | WP_003234144.1 |
| HEIKHJEJ_13 | 51128 | 51646 | satA | streptothricin N-acetyltransferase SatA | BLASTX | 90.75 | 100.00 | WP_003242546.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| HEIKHJEJ_55 | 29737 | 30918 | capB | 0.0 | Capsule | Immune modulation | 99.66 | 100.00 | NP_391471 |
| HEIKHJEJ_11 | 23035 | 23820 | dhbA | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.49 | 100.00 | NP_391080 |
| HEIKHJEJ_55 | 28112 | 29254 | capA | 0.0 | Capsule | Immune modulation | 99.30 | 100.00 | NP_391469 |
| HEIKHJEJ_11 | 27676 | 34812 | dhbF | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.30 | 100.00 | NP_391076 |
| HEIKHJEJ_38 | 1521 | 3284 | dep/capD | 0.0 | Capsule | Immune modulation | 99.04 | 100.00 | NP_389723 |
| HEIKHJEJ_11 | 26718 | 27656 | dhbB | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.04 | 100.00 | NP_391077 |
| HEIKHJEJ_11 | 23846 | 25042 | dhbC | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.00 | 100.00 | NP_391079 |
| HEIKHJEJ_12 | 15353 | 15994 | hlyIII | 0.0 | Hemolysin III | Exotoxin | 98.91 | 100.00 | NP_390062 |
| HEIKHJEJ_55 | 29273 | 29722 | capC | 0.0 | Capsule | Immune modulation | 98.89 | 100.00 | NP_391470 |
| HEIKHJEJ_11 | 25071 | 26690 | dhbE | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 96.73 | 100.00 | NP_391078 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| HEIKHJEJ_36 | 28062 | 26926 | tufA | 0.0 | elongation factor Tu | 84.96 | 95.69 | AHM69299 |
| HEIKHJEJ_86 | 11309 | 10440 | pdxS | 1.83e-151 | encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS | 83.45 | 98.31 | ACE61268 |
| HEIKHJEJ_8 | 96868 | 96092 | CodY | 2.01e-125 | nutrient-responsive regulator | 81.08 | 100.00 | ACJ80679 |
| HEIKHJEJ_62 | 4348 | 4917 | ClpP | 2.96e-100 | part of proteolytic complex | 81.05 | 97.44 | BAB94595 |
| HEIKHJEJ_18 | 41554 | 41162 | spxA1 | 4.13e-71 | redox-responsive transcription factor | 83.97 | 100.00 | AAF21893 |
| HEIKHJEJ_18 | 41554 | 41162 | spx | 2.46e-69 | essential gene which is a thiol/oxidative stress sensor | 80.15 | 100.00 | BAB57159 |
| HEIKHJEJ_30 | 34674 | 34432 | SpoVG | 7.52e-39 | RNA-binding protein | 81.48 | 79.41 | AAT02994 |
| HEIKHJEJ_21 | 9941 | 9744 | CspA | 1.03e-32 | cold shock protein | 90.91 | 100.00 | CAA62903 |
| HEIKHJEJ_12 | 27224 | 27418 | CspD | 8.86e-29 | cold shock protein | 81.54 | 98.48 | CAC99957 |
| HEIKHJEJ_17 | 32203 | 32009 | CspB | 4.13e-28 | cold shock protein | 83.08 | 98.48 | CAD00094 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| HEIKHJEJ_46 | 1449 | 1616 | Col(SD853) | 168 / 194 | 86.90 | NC015392 |
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| HEIKHJEJ_1 | 0.0 | 1.0 | 0.0 |
| HEIKHJEJ_2 | 0.0 | 1.0 | 0.0 |
| HEIKHJEJ_3 | 0.0 | 1.0 | 0.0 |
| HEIKHJEJ_4 | 0.0 | 1.0 | 0.0 |
| HEIKHJEJ_5 | 0.0 | 1.0 | 0.0 |
| HEIKHJEJ_6 | 0.0 | 1.0 | 0.0 |
| HEIKHJEJ_7 | 0.0 | 1.0 | 0.0 |
| HEIKHJEJ_8 | 0.0 | 1.0 | 0.0 |
| HEIKHJEJ_9 | 0.0 | 1.0 | 0.0 |
| HEIKHJEJ_10 | 0.0 | 1.0 | 0.0 |
| HEIKHJEJ_11 | 0.0 | 1.0 | 0.0 |
| HEIKHJEJ_12 | 0.0 | 1.0 | 0.0 |
| HEIKHJEJ_13 | 0.0 | 1.0 | 0.0 |
| HEIKHJEJ_14 | 0.0 | 1.0 | 0.0 |
| HEIKHJEJ_15 | 0.0 | 1.0 | 0.0 |
| HEIKHJEJ_16 | 0.0 | 1.0 | 0.0 |
| HEIKHJEJ_17 | 0.0 | 1.0 | 0.0 |
| HEIKHJEJ_18 | 0.0 | 1.0 | 0.0 |
| HEIKHJEJ_19 | 0.0 | 1.0 | 0.0 |
| HEIKHJEJ_20 | 0.0 | 1.0 | 0.0 |
| HEIKHJEJ_21 | 0.0 | 1.0 | 0.0 |
| HEIKHJEJ_22 | 0.0 | 1.0 | 0.0 |
| HEIKHJEJ_23 | 0.0 | 1.0 | 0.0 |
| HEIKHJEJ_24 | 0.0 | 1.0 | 0.0 |
| HEIKHJEJ_25 | 0.0 | 1.0 | 0.0 |
| HEIKHJEJ_26 | 0.0 | 1.0 | 0.0 |
| HEIKHJEJ_27 | 0.0 | 1.0 | 0.0 |
| HEIKHJEJ_28 | 0.0 | 1.0 | 0.0 |
| HEIKHJEJ_29 | 0.0 | 1.0 | 0.0 |
| HEIKHJEJ_30 | 0.0 | 1.0 | 0.0 |
| HEIKHJEJ_31 | 0.0 | 1.0 | 0.0 |
| HEIKHJEJ_32 | 0.0 | 1.0 | 0.0 |
| HEIKHJEJ_33 | 0.0 | 1.0 | 0.0 |
| HEIKHJEJ_34 | 0.0 | 1.0 | 0.0 |
| HEIKHJEJ_35 | 0.0 | 1.0 | 0.0 |
| HEIKHJEJ_36 | 0.0 | 1.0 | 0.0 |
| HEIKHJEJ_37 | 0.0 | 1.0 | 0.0 |
| HEIKHJEJ_38 | 0.0 | 1.0 | 0.0 |
| HEIKHJEJ_39 | 0.0 | 1.0 | 0.0 |
| HEIKHJEJ_40 | 0.0 | 1.0 | 0.0 |
| HEIKHJEJ_41 | 0.0 | 1.0 | 0.0 |
| HEIKHJEJ_42 | 0.0 | 1.0 | 0.0 |
| HEIKHJEJ_43 | 0.0 | 1.0 | 0.0 |
| HEIKHJEJ_44 | 0.0 | 1.0 | 0.0 |
| HEIKHJEJ_45 | 0.0 | 1.0 | 0.0 |
| HEIKHJEJ_46 | 1.0 | 0.0 | 1.0 |
| HEIKHJEJ_47 | 0.0 | 1.0 | 0.0 |
| HEIKHJEJ_48 | 0.0 | 1.0 | 0.0 |
| HEIKHJEJ_49 | 0.0 | 1.0 | 0.0 |
| HEIKHJEJ_50 | 0.0 | 1.0 | 0.0 |
| HEIKHJEJ_51 | 0.0 | 1.0 | 0.0 |
| HEIKHJEJ_52 | 0.0 | 1.0 | 0.0 |
| HEIKHJEJ_53 | 0.0 | 1.0 | 0.0 |
| HEIKHJEJ_54 | 0.0 | 1.0 | 0.0 |
| HEIKHJEJ_55 | 0.0 | 1.0 | 0.0 |
| HEIKHJEJ_56 | 1.0 | 0.0 | 1.0 |
| HEIKHJEJ_57 | 0.0 | 1.0 | 0.0 |
| HEIKHJEJ_58 | 0.0 | 1.0 | 0.0 |
| HEIKHJEJ_59 | 0.0 | 1.0 | 0.0 |
| HEIKHJEJ_61 | 0.0 | 1.0 | 0.0 |
| HEIKHJEJ_62 | 0.0 | 1.0 | 0.0 |
| HEIKHJEJ_63 | 0.0 | 1.0 | 0.0 |
| HEIKHJEJ_64 | 0.0 | 1.0 | 0.0 |
| HEIKHJEJ_65 | 1.0 | 0.0 | 1.0 |
| HEIKHJEJ_66 | 0.0 | 1.0 | 0.0 |
| HEIKHJEJ_67 | 0.0 | 1.0 | 0.0 |
| HEIKHJEJ_68 | 0.0 | 1.0 | 0.0 |
| HEIKHJEJ_69 | 0.0 | 1.0 | 0.0 |
| HEIKHJEJ_70 | 0.0 | 1.0 | 0.0 |
| HEIKHJEJ_71 | 0.0 | 1.0 | 0.0 |
| HEIKHJEJ_72 | 1.0 | 0.0 | 1.0 |
| HEIKHJEJ_73 | 0.0 | 1.0 | 0.0 |
| HEIKHJEJ_74 | 0.0 | 1.0 | 0.0 |
| HEIKHJEJ_75 | 0.0 | 1.0 | 0.0 |
| HEIKHJEJ_76 | 0.0 | 1.0 | 0.0 |
| HEIKHJEJ_77 | 0.0 | 1.0 | 0.0 |
| HEIKHJEJ_78 | 0.0 | 1.0 | 0.0 |
| HEIKHJEJ_79 | 0.0 | 1.0 | 0.0 |
| HEIKHJEJ_80 | 0.0 | 1.0 | 0.0 |
| HEIKHJEJ_81 | 0.0 | 1.0 | 0.0 |
| HEIKHJEJ_82 | 0.0 | 1.0 | 0.0 |
| HEIKHJEJ_83 | 0.0 | 1.0 | 0.0 |
| HEIKHJEJ_85 | 1.0 | 0.0 | 1.0 |
| HEIKHJEJ_86 | 0.0 | 1.0 | 0.0 |
| HEIKHJEJ_87 | 1.0 | 0.0 | 1.0 |
| HEIKHJEJ_88 | 1.0 | 0.0 | 1.0 |
| HEIKHJEJ_89 | 0.0 | 1.0 | 0.0 |
| HEIKHJEJ_90 | 1.0 | 0.0 | 1.0 |
| HEIKHJEJ_91 | 0.0 | 1.0 | 0.0 |
| HEIKHJEJ_94 | 0.0 | 1.0 | 0.0 |
| HEIKHJEJ_95 | 1.0 | 0.0 | 1.0 |
| HEIKHJEJ_96 | 1.0 | 0.0 | 1.0 |
| HEIKHJEJ_97 | 0.0 | 1.0 | 0.0 |
| HEIKHJEJ_98 | 0.0 | 1.0 | 0.0 |
| HEIKHJEJ_99 | 0.0 | 1.0 | 0.0 |
| HEIKHJEJ_100 | 0.0 | 1.0 | 0.0 |
| HEIKHJEJ_101 | 0.0 | 1.0 | 0.0 |
| HEIKHJEJ_102 | 0.0 | 1.0 | 0.0 |
| HEIKHJEJ_103 | 1.0 | 0.0 | 1.0 |
| HEIKHJEJ_104 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| HEIKHJEJ_2 | 5821 | 32504 | False | Myoviridae | VOG9667, VOG3490, VOG0189, VOG4843, VOG5447, VOG0796, VOG10227, VOG4773, VOG4685, VOG0749, VOG3890, VOG3891, VOG3892, VOG6163, VOG4865, VOG4885, VOG3894, VOG4550, VOG4691, VOG4862, VOG7718, VOG4705 |
| HEIKHJEJ_20 | 8195 | 18725 | True | Siphoviridae | VOG6587, VOG0189, VOG4566, VOG7581, VOG9667, VOG9667, VOG1563, VOG0275, VOG0275 |
| HEIKHJEJ_33 | 2 | 27505 | False | Siphoviridae | VOG4612, VOG4626, VOG4619, VOG0801, VOG6163, VOG5910, VOG0541, VOG4586, VOG0704, VOG0539, VOG4589, VOG0204, VOG5361, VOG4553, VOG4568, VOG4556, VOG3644, VOG4544, VOG4581, VOG7403, VOG4841, VOG0198, VOG7372, VOG2752, VOG4606, VOG1227, VOG1936, VOG0535, VOG0650 |
Phigaro v2.4.0 (Database: Jan. 2024 release)