Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 10 | 10 | 9 | 16.76 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| BMPIAKOO_20 | 60972 | 62174 | blt | ARO:3003006 | blt | 98.00 | 100.00 | L32599.1 |
| BMPIAKOO_27 | 59379 | 61949 | Bacillus subtilis mprF | ARO:3003324 | Bsub_mprF | 99.53 | 100.00 | AL009126.3 |
| BMPIAKOO_20 | 44223 | 45077 | aadK | ARO:3002627 | aadK | 98.59 | 100.00 | AL009126.1 |
| BMPIAKOO_43 | 41006 | 41926 | mphK | ARO:3004541 | mphK | 98.68 | 100.00 | CP010314.1 |
| BMPIAKOO_13 | 40810 | 41148 | ykkC | ARO:3003063 | ykkC | 98.21 | 100.00 | AL009126.1 |
| BMPIAKOO_13 | 40493 | 40810 | ykkD | ARO:3003064 | ykkD | 98.10 | 100.00 | AL009126.1 |
| BMPIAKOO_43 | 27672 | 29111 | lmrB | ARO:3002813 | lmrB | 99.58 | 100.42 | JYFL01000006.1 |
| BMPIAKOO_70 | 5724 | 7370 | vmlR | ARO:3004476 | vmlR | 98.36 | 100.18 | NC_000964.3 |
| BMPIAKOO_57 | 872 | 1465 | tmrB | ARO:3003059 | tmrB | 99.49 | 100.00 | AL009126.3 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| BMPIAKOO_20 | 44223 | 45077 | aadK | Streptomycin | 98.83 | 100.00 | M26879 |
| BMPIAKOO_43 | 41006 | 41926 | mph(K) | Spiramycin, Telithromycin | 98.05 | 100.00 | NC_000964 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| BMPIAKOO_43 | 41018 | 41926 | mphK | macrolide 2'-phosphotransferase MphK | BLASTX | 98.68 | 99.02 | WP_003246254.1 |
| BMPIAKOO_20 | 44223 | 45074 | aadK | aminoglycoside 6-adenylyltransferase AadK | BLASTX | 98.59 | 100.00 | WP_003229862.1 |
| BMPIAKOO_70 | 5727 | 7370 | vmlR | ABC-F type ribosomal protection protein VmlR | BLASTX | 98.36 | 100.00 | WP_003234144.1 |
| BMPIAKOO_30 | 51143 | 51661 | satA | streptothricin N-acetyltransferase SatA | BLASTX | 90.75 | 100.00 | WP_003242546.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| BMPIAKOO_22 | 8177 | 9358 | capB | 0.0 | Capsule | Immune modulation | 99.66 | 100.00 | NP_391471 |
| BMPIAKOO_48 | 36172 | 36957 | dhbA | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.49 | 100.00 | NP_391080 |
| BMPIAKOO_22 | 9841 | 10983 | capA | 0.0 | Capsule | Immune modulation | 99.30 | 100.00 | NP_391469 |
| BMPIAKOO_48 | 25180 | 32316 | dhbF | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.30 | 100.00 | NP_391076 |
| BMPIAKOO_50 | 19698 | 21461 | dep/capD | 0.0 | Capsule | Immune modulation | 99.04 | 100.00 | NP_389723 |
| BMPIAKOO_48 | 32336 | 33274 | dhbB | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.04 | 100.00 | NP_391077 |
| BMPIAKOO_48 | 34950 | 36146 | dhbC | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.00 | 100.00 | NP_391079 |
| BMPIAKOO_76 | 32545 | 33186 | hlyIII | 0.0 | Hemolysin III | Exotoxin | 98.91 | 100.00 | NP_390062 |
| BMPIAKOO_22 | 9373 | 9822 | capC | 0.0 | Capsule | Immune modulation | 98.89 | 100.00 | NP_391470 |
| BMPIAKOO_48 | 33302 | 34921 | dhbE | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 96.73 | 100.00 | NP_391078 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| BMPIAKOO_33 | 28055 | 26919 | tufA | 0.0 | elongation factor Tu | 84.96 | 95.69 | AHM69299 |
| BMPIAKOO_4 | 4400 | 5269 | pdxS | 1.97e-151 | encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS | 83.45 | 98.31 | ACE61268 |
| BMPIAKOO_1 | 94222 | 93446 | CodY | 1.99e-125 | nutrient-responsive regulator | 81.08 | 100.00 | ACJ80679 |
| BMPIAKOO_54 | 45211 | 44642 | ClpP | 2.96e-100 | part of proteolytic complex | 81.05 | 97.44 | BAB94595 |
| BMPIAKOO_25 | 41760 | 41368 | spxA1 | 3.19e-71 | redox-responsive transcription factor | 83.97 | 100.00 | AAF21893 |
| BMPIAKOO_25 | 41760 | 41368 | spx | 1.90e-69 | essential gene which is a thiol/oxidative stress sensor | 80.15 | 100.00 | BAB57159 |
| BMPIAKOO_36 | 20678 | 20920 | SpoVG | 7.53e-39 | RNA-binding protein | 81.48 | 79.41 | AAT02994 |
| BMPIAKOO_18 | 40461 | 40264 | CspA | 2.35e-32 | cold shock protein | 90.91 | 100.00 | CAA62903 |
| BMPIAKOO_76 | 44416 | 44610 | CspD | 3.50e-29 | cold shock protein | 81.54 | 98.48 | CAC99957 |
| BMPIAKOO_5 | 32160 | 31966 | CspB | 3.37e-28 | cold shock protein | 83.08 | 98.48 | CAD00094 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| BMPIAKOO_17 | 903 | 1070 | Col(SD853) | 168 / 194 | 86.90 | NC015392 |
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| BMPIAKOO_1 | 0.0 | 1.0 | 0.0 |
| BMPIAKOO_2 | 0.0 | 1.0 | 0.0 |
| BMPIAKOO_3 | 0.0 | 1.0 | 0.0 |
| BMPIAKOO_4 | 0.0 | 1.0 | 0.0 |
| BMPIAKOO_5 | 0.0 | 1.0 | 0.0 |
| BMPIAKOO_6 | 0.0 | 1.0 | 0.0 |
| BMPIAKOO_7 | 0.0 | 1.0 | 0.0 |
| BMPIAKOO_8 | 1.0 | 0.0 | 1.0 |
| BMPIAKOO_9 | 0.0 | 1.0 | 0.0 |
| BMPIAKOO_10 | 0.0 | 1.0 | 0.0 |
| BMPIAKOO_11 | 0.0 | 1.0 | 0.0 |
| BMPIAKOO_12 | 0.0 | 1.0 | 0.0 |
| BMPIAKOO_13 | 0.0 | 1.0 | 0.0 |
| BMPIAKOO_14 | 0.0 | 1.0 | 0.0 |
| BMPIAKOO_15 | 0.0 | 1.0 | 0.0 |
| BMPIAKOO_16 | 0.0 | 1.0 | 0.0 |
| BMPIAKOO_17 | 1.0 | 0.0 | 1.0 |
| BMPIAKOO_18 | 0.0 | 1.0 | 0.0 |
| BMPIAKOO_19 | 0.0 | 1.0 | 0.0 |
| BMPIAKOO_20 | 0.0 | 1.0 | 0.0 |
| BMPIAKOO_21 | 0.0 | 1.0 | 0.0 |
| BMPIAKOO_22 | 0.0 | 1.0 | 0.0 |
| BMPIAKOO_23 | 0.0 | 1.0 | 0.0 |
| BMPIAKOO_24 | 1.0 | 0.0 | 1.0 |
| BMPIAKOO_25 | 0.0 | 1.0 | 0.0 |
| BMPIAKOO_26 | 0.0 | 1.0 | 0.0 |
| BMPIAKOO_27 | 0.0 | 1.0 | 0.0 |
| BMPIAKOO_28 | 0.0 | 1.0 | 0.0 |
| BMPIAKOO_29 | 0.0 | 1.0 | 0.0 |
| BMPIAKOO_30 | 0.0 | 1.0 | 0.0 |
| BMPIAKOO_31 | 0.0 | 1.0 | 0.0 |
| BMPIAKOO_32 | 0.0 | 1.0 | 0.0 |
| BMPIAKOO_33 | 0.0 | 1.0 | 0.0 |
| BMPIAKOO_34 | 1.0 | 0.0 | 1.0 |
| BMPIAKOO_35 | 0.0 | 1.0 | 0.0 |
| BMPIAKOO_36 | 0.0 | 1.0 | 0.0 |
| BMPIAKOO_37 | 0.0 | 1.0 | 0.0 |
| BMPIAKOO_38 | 0.0 | 1.0 | 0.0 |
| BMPIAKOO_40 | 0.0 | 1.0 | 0.0 |
| BMPIAKOO_41 | 0.0 | 1.0 | 0.0 |
| BMPIAKOO_42 | 0.0 | 1.0 | 0.0 |
| BMPIAKOO_43 | 0.0 | 1.0 | 0.0 |
| BMPIAKOO_44 | 0.0 | 1.0 | 0.0 |
| BMPIAKOO_45 | 0.0 | 1.0 | 0.0 |
| BMPIAKOO_46 | 0.0 | 1.0 | 0.0 |
| BMPIAKOO_47 | 0.0 | 1.0 | 0.0 |
| BMPIAKOO_48 | 0.0 | 1.0 | 0.0 |
| BMPIAKOO_49 | 0.0 | 1.0 | 0.0 |
| BMPIAKOO_50 | 0.0 | 1.0 | 0.0 |
| BMPIAKOO_51 | 0.0 | 1.0 | 0.0 |
| BMPIAKOO_52 | 0.0 | 1.0 | 0.0 |
| BMPIAKOO_53 | 0.0 | 1.0 | 0.0 |
| BMPIAKOO_54 | 0.0 | 1.0 | 0.0 |
| BMPIAKOO_55 | 0.0 | 1.0 | 0.0 |
| BMPIAKOO_56 | 0.0 | 1.0 | 0.0 |
| BMPIAKOO_57 | 0.0 | 1.0 | 0.0 |
| BMPIAKOO_58 | 0.0 | 1.0 | 0.0 |
| BMPIAKOO_59 | 0.0 | 1.0 | 0.0 |
| BMPIAKOO_60 | 1.0 | 0.0 | 1.0 |
| BMPIAKOO_61 | 0.0 | 1.0 | 0.0 |
| BMPIAKOO_62 | 0.0 | 1.0 | 0.0 |
| BMPIAKOO_63 | 0.0 | 1.0 | 0.0 |
| BMPIAKOO_64 | 0.0 | 1.0 | 0.0 |
| BMPIAKOO_65 | 0.0 | 1.0 | 0.0 |
| BMPIAKOO_66 | 0.0 | 1.0 | 0.0 |
| BMPIAKOO_67 | 0.0 | 1.0 | 0.0 |
| BMPIAKOO_68 | 0.0 | 1.0 | 0.0 |
| BMPIAKOO_69 | 0.0 | 1.0 | 0.0 |
| BMPIAKOO_70 | 0.0 | 1.0 | 0.0 |
| BMPIAKOO_71 | 0.0 | 1.0 | 0.0 |
| BMPIAKOO_72 | 1.0 | 0.0 | 1.0 |
| BMPIAKOO_73 | 1.0 | 0.0 | 1.0 |
| BMPIAKOO_74 | 1.0 | 0.0 | 1.0 |
| BMPIAKOO_75 | 0.0 | 1.0 | 0.0 |
| BMPIAKOO_76 | 0.0 | 1.0 | 0.0 |
| BMPIAKOO_77 | 0.0 | 1.0 | 0.0 |
| BMPIAKOO_78 | 0.0 | 1.0 | 0.0 |
| BMPIAKOO_79 | 0.0 | 1.0 | 0.0 |
| BMPIAKOO_80 | 0.0 | 1.0 | 0.0 |
| BMPIAKOO_81 | 0.0 | 1.0 | 0.0 |
| BMPIAKOO_82 | 0.0 | 1.0 | 0.0 |
| BMPIAKOO_83 | 1.0 | 0.0 | 1.0 |
| BMPIAKOO_84 | 0.0 | 1.0 | 0.0 |
| BMPIAKOO_85 | 1.0 | 0.0 | 1.0 |
| BMPIAKOO_86 | 1.0 | 0.0 | 1.0 |
| BMPIAKOO_87 | 0.0 | 1.0 | 0.0 |
| BMPIAKOO_88 | 0.0 | 1.0 | 0.0 |
| BMPIAKOO_89 | 0.0 | 1.0 | 0.0 |
| BMPIAKOO_92 | 1.0 | 0.0 | 1.0 |
| BMPIAKOO_93 | 0.0 | 1.0 | 0.0 |
| BMPIAKOO_94 | 1.0 | 0.0 | 1.0 |
| BMPIAKOO_95 | 0.0 | 1.0 | 0.0 |
| BMPIAKOO_96 | 0.0 | 1.0 | 0.0 |
| BMPIAKOO_97 | 0.0 | 1.0 | 0.0 |
| BMPIAKOO_98 | 1.0 | 0.0 | 1.0 |
| BMPIAKOO_99 | 0.0 | 1.0 | 0.0 |
| BMPIAKOO_101 | 1.0 | 0.0 | 1.0 |
| BMPIAKOO_102 | 0.0 | 1.0 | 0.0 |
| BMPIAKOO_105 | 1.0 | 0.0 | 1.0 |
| BMPIAKOO_106 | 0.0 | 1.0 | 0.0 |
| BMPIAKOO_107 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| BMPIAKOO_13 | 70612 | 97295 | False | Myoviridae | VOG0753, VOG4705, VOG7718, VOG4862, VOG4691, VOG4550, VOG3894, VOG4885, VOG4865, VOG6163, VOG3892, VOG3891, VOG3890, VOG0749, VOG4685, VOG4773, VOG10227, VOG0796, VOG5447, VOG4843, VOG0189, VOG3490 |
| BMPIAKOO_15 | 1 | 27489 | False | Siphoviridae | VOG4612, VOG4626, VOG4619, VOG0801, VOG6163, VOG5910, VOG0541, VOG4586, VOG0704, VOG0539, VOG4589, VOG0204, VOG5361, VOG4553, VOG4568, VOG4556, VOG3644, VOG4544, VOG4581, VOG7403, VOG4841, VOG0198, VOG7372, VOG2752, VOG4606, VOG1227, VOG1936, VOG0535, VOG0650 |
| BMPIAKOO_45 | 4690 | 15220 | True | Siphoviridae | VOG6587, VOG0189, VOG4566, VOG7581, VOG9667, VOG9667, VOG1563, VOG0275, VOG0275 |
Phigaro v2.4.0 (Database: Jan. 2024 release)