Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 10 | 10 | 9 | 16.76 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| FFCFBHGA_4 | 107292 | 108731 | lmrB | ARO:3002813 | lmrB | 99.58 | 100.42 | JYFL01000006.1 |
| FFCFBHGA_4 | 94477 | 95397 | mphK | ARO:3004541 | mphK | 98.68 | 100.00 | CP010314.1 |
| FFCFBHGA_65 | 62306 | 62623 | ykkD | ARO:3003064 | ykkD | 98.10 | 100.00 | AL009126.1 |
| FFCFBHGA_65 | 61968 | 62306 | ykkC | ARO:3003063 | ykkC | 98.21 | 100.00 | AL009126.1 |
| FFCFBHGA_28 | 59379 | 61949 | Bacillus subtilis mprF | ARO:3003324 | Bsub_mprF | 99.53 | 100.00 | AL009126.3 |
| FFCFBHGA_2 | 28679 | 30325 | vmlR | ARO:3004476 | vmlR | 98.36 | 100.18 | NC_000964.3 |
| FFCFBHGA_49 | 23943 | 24797 | aadK | ARO:3002627 | aadK | 98.59 | 100.00 | AL009126.1 |
| FFCFBHGA_49 | 6846 | 8048 | blt | ARO:3003006 | blt | 98.00 | 100.00 | L32599.1 |
| FFCFBHGA_50 | 872 | 1465 | tmrB | ARO:3003059 | tmrB | 99.49 | 100.00 | AL009126.3 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| FFCFBHGA_49 | 23943 | 24797 | aadK | Streptomycin | 98.83 | 100.00 | M26879 |
| FFCFBHGA_4 | 94477 | 95397 | mph(K) | Spiramycin, Telithromycin | 98.05 | 100.00 | NC_000964 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| FFCFBHGA_4 | 94477 | 95385 | mphK | macrolide 2'-phosphotransferase MphK | BLASTX | 98.68 | 99.02 | WP_003246254.1 |
| FFCFBHGA_49 | 23946 | 24797 | aadK | aminoglycoside 6-adenylyltransferase AadK | BLASTX | 98.59 | 100.00 | WP_003229862.1 |
| FFCFBHGA_2 | 28679 | 30322 | vmlR | ABC-F type ribosomal protection protein VmlR | BLASTX | 98.36 | 100.00 | WP_003234144.1 |
| FFCFBHGA_25 | 51107 | 51625 | satA | streptothricin N-acetyltransferase SatA | BLASTX | 90.75 | 100.00 | WP_003242546.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| FFCFBHGA_17 | 226275 | 227456 | capB | 0.0 | Capsule | Immune modulation | 99.66 | 100.00 | NP_391471 |
| FFCFBHGA_46 | 23051 | 23836 | dhbA | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.49 | 100.00 | NP_391080 |
| FFCFBHGA_17 | 227939 | 229081 | capA | 0.0 | Capsule | Immune modulation | 99.30 | 100.00 | NP_391469 |
| FFCFBHGA_46 | 27692 | 34828 | dhbF | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.30 | 100.00 | NP_391076 |
| FFCFBHGA_80 | 19698 | 21461 | dep/capD | 0.0 | Capsule | Immune modulation | 99.04 | 100.00 | NP_389723 |
| FFCFBHGA_46 | 26734 | 27672 | dhbB | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.04 | 100.00 | NP_391077 |
| FFCFBHGA_46 | 23862 | 25058 | dhbC | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.00 | 100.00 | NP_391079 |
| FFCFBHGA_58 | 143841 | 144482 | hlyIII | 0.0 | Hemolysin III | Exotoxin | 98.91 | 100.00 | NP_390062 |
| FFCFBHGA_17 | 227471 | 227920 | capC | 0.0 | Capsule | Immune modulation | 98.89 | 100.00 | NP_391470 |
| FFCFBHGA_46 | 25087 | 26706 | dhbE | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 96.73 | 100.00 | NP_391078 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| FFCFBHGA_53 | 31898 | 33034 | tufA | 0.0 | elongation factor Tu | 84.96 | 95.69 | AHM69299 |
| FFCFBHGA_72 | 11364 | 10495 | pdxS | 1.98e-151 | encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS | 83.45 | 98.31 | ACE61268 |
| FFCFBHGA_11 | 151348 | 152124 | CodY | 1.99e-125 | nutrient-responsive regulator | 81.08 | 100.00 | ACJ80679 |
| FFCFBHGA_27 | 45211 | 44642 | ClpP | 2.96e-100 | part of proteolytic complex | 81.05 | 97.44 | BAB94595 |
| FFCFBHGA_24 | 9681 | 10073 | spxA1 | 3.25e-71 | redox-responsive transcription factor | 83.97 | 100.00 | AAF21893 |
| FFCFBHGA_24 | 9681 | 10073 | spx | 1.94e-69 | essential gene which is a thiol/oxidative stress sensor | 80.15 | 100.00 | BAB57159 |
| FFCFBHGA_34 | 20519 | 20761 | SpoVG | 7.48e-39 | RNA-binding protein | 81.48 | 79.41 | AAT02994 |
| FFCFBHGA_69 | 13847 | 14044 | CspA | 2.34e-32 | cold shock protein | 90.91 | 100.00 | CAA62903 |
| FFCFBHGA_58 | 132611 | 132417 | CspD | 1.09e-28 | cold shock protein | 81.54 | 98.48 | CAC99957 |
| FFCFBHGA_14 | 13226 | 13420 | CspB | 4.15e-28 | cold shock protein | 83.08 | 98.48 | CAD00094 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| FFCFBHGA_56 | 1754 | 1921 | Col(SD853) | 168 / 194 | 86.90 | NC015392 |
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| FFCFBHGA_1 | 0.0 | 1.0 | 0.0 |
| FFCFBHGA_2 | 0.0 | 1.0 | 0.0 |
| FFCFBHGA_3 | 0.0 | 1.0 | 0.0 |
| FFCFBHGA_4 | 0.0 | 1.0 | 0.0 |
| FFCFBHGA_5 | 0.0 | 1.0 | 0.0 |
| FFCFBHGA_6 | 0.0 | 1.0 | 0.0 |
| FFCFBHGA_7 | 0.0 | 1.0 | 0.0 |
| FFCFBHGA_8 | 1.0 | 0.0 | 1.0 |
| FFCFBHGA_10 | 0.0 | 1.0 | 0.0 |
| FFCFBHGA_11 | 0.0 | 1.0 | 0.0 |
| FFCFBHGA_12 | 0.0 | 1.0 | 0.0 |
| FFCFBHGA_13 | 0.0 | 1.0 | 0.0 |
| FFCFBHGA_14 | 0.0 | 1.0 | 0.0 |
| FFCFBHGA_15 | 0.0 | 1.0 | 0.0 |
| FFCFBHGA_16 | 0.0 | 1.0 | 0.0 |
| FFCFBHGA_17 | 0.0 | 1.0 | 0.0 |
| FFCFBHGA_18 | 0.0 | 1.0 | 0.0 |
| FFCFBHGA_19 | 0.0 | 1.0 | 0.0 |
| FFCFBHGA_20 | 0.0 | 1.0 | 0.0 |
| FFCFBHGA_21 | 0.0 | 1.0 | 0.0 |
| FFCFBHGA_22 | 0.0 | 1.0 | 0.0 |
| FFCFBHGA_23 | 1.0 | 0.0 | 1.0 |
| FFCFBHGA_24 | 0.0 | 1.0 | 0.0 |
| FFCFBHGA_25 | 0.0 | 1.0 | 0.0 |
| FFCFBHGA_26 | 0.0 | 1.0 | 0.0 |
| FFCFBHGA_27 | 0.0 | 1.0 | 0.0 |
| FFCFBHGA_28 | 0.0 | 1.0 | 0.0 |
| FFCFBHGA_29 | 0.0 | 1.0 | 0.0 |
| FFCFBHGA_31 | 0.0 | 1.0 | 0.0 |
| FFCFBHGA_32 | 0.0 | 1.0 | 0.0 |
| FFCFBHGA_34 | 0.0 | 1.0 | 0.0 |
| FFCFBHGA_35 | 0.0 | 1.0 | 0.0 |
| FFCFBHGA_36 | 0.0 | 1.0 | 0.0 |
| FFCFBHGA_37 | 0.0 | 1.0 | 0.0 |
| FFCFBHGA_38 | 0.0 | 1.0 | 0.0 |
| FFCFBHGA_39 | 0.0 | 1.0 | 0.0 |
| FFCFBHGA_40 | 0.0 | 1.0 | 0.0 |
| FFCFBHGA_41 | 0.0 | 1.0 | 0.0 |
| FFCFBHGA_42 | 0.0 | 1.0 | 0.0 |
| FFCFBHGA_43 | 0.0 | 1.0 | 0.0 |
| FFCFBHGA_44 | 0.0 | 1.0 | 0.0 |
| FFCFBHGA_45 | 0.0 | 1.0 | 0.0 |
| FFCFBHGA_46 | 0.0 | 1.0 | 0.0 |
| FFCFBHGA_47 | 0.0 | 1.0 | 0.0 |
| FFCFBHGA_48 | 0.0 | 1.0 | 0.0 |
| FFCFBHGA_49 | 0.0 | 1.0 | 0.0 |
| FFCFBHGA_50 | 0.0 | 1.0 | 0.0 |
| FFCFBHGA_51 | 0.0 | 1.0 | 0.0 |
| FFCFBHGA_52 | 0.0 | 1.0 | 0.0 |
| FFCFBHGA_53 | 0.0 | 1.0 | 0.0 |
| FFCFBHGA_54 | 0.0 | 1.0 | 0.0 |
| FFCFBHGA_55 | 0.0 | 1.0 | 0.0 |
| FFCFBHGA_56 | 1.0 | 0.0 | 1.0 |
| FFCFBHGA_57 | 0.0 | 1.0 | 0.0 |
| FFCFBHGA_58 | 0.0 | 1.0 | 0.0 |
| FFCFBHGA_59 | 1.0 | 0.0 | 1.0 |
| FFCFBHGA_60 | 0.0 | 1.0 | 0.0 |
| FFCFBHGA_61 | 1.0 | 0.0 | 1.0 |
| FFCFBHGA_62 | 0.0 | 1.0 | 0.0 |
| FFCFBHGA_63 | 0.0 | 1.0 | 0.0 |
| FFCFBHGA_64 | 0.0 | 1.0 | 0.0 |
| FFCFBHGA_65 | 0.0 | 1.0 | 0.0 |
| FFCFBHGA_66 | 0.0 | 1.0 | 0.0 |
| FFCFBHGA_67 | 0.0 | 1.0 | 0.0 |
| FFCFBHGA_68 | 0.0 | 1.0 | 0.0 |
| FFCFBHGA_69 | 0.0 | 1.0 | 0.0 |
| FFCFBHGA_70 | 0.0 | 1.0 | 0.0 |
| FFCFBHGA_71 | 0.0 | 1.0 | 0.0 |
| FFCFBHGA_72 | 0.0 | 1.0 | 0.0 |
| FFCFBHGA_73 | 1.0 | 0.0 | 1.0 |
| FFCFBHGA_75 | 0.0 | 1.0 | 0.0 |
| FFCFBHGA_76 | 0.0 | 1.0 | 0.0 |
| FFCFBHGA_77 | 0.0 | 1.0 | 0.0 |
| FFCFBHGA_78 | 0.0 | 1.0 | 0.0 |
| FFCFBHGA_79 | 0.0 | 1.0 | 0.0 |
| FFCFBHGA_80 | 0.0 | 1.0 | 0.0 |
| FFCFBHGA_81 | 0.0 | 1.0 | 0.0 |
| FFCFBHGA_82 | 1.0 | 0.0 | 1.0 |
| FFCFBHGA_83 | 1.0 | 0.0 | 1.0 |
| FFCFBHGA_84 | 0.0 | 1.0 | 0.0 |
| FFCFBHGA_85 | 0.0 | 1.0 | 0.0 |
| FFCFBHGA_86 | 0.0 | 1.0 | 0.0 |
| FFCFBHGA_87 | 0.0 | 1.0 | 0.0 |
| FFCFBHGA_88 | 1.0 | 0.0 | 1.0 |
| FFCFBHGA_89 | 1.0 | 0.0 | 1.0 |
| FFCFBHGA_91 | 1.0 | 0.0 | 1.0 |
| FFCFBHGA_92 | 1.0 | 0.0 | 1.0 |
| FFCFBHGA_93 | 1.0 | 0.0 | 1.0 |
| FFCFBHGA_94 | 0.0 | 1.0 | 0.0 |
| FFCFBHGA_96 | 1.0 | 0.0 | 1.0 |
| FFCFBHGA_97 | 1.0 | 0.0 | 1.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| FFCFBHGA_20 | 1 | 25830 | False | Siphoviridae | VOG4626, VOG4619, VOG0801, VOG6163, VOG5910, VOG0541, VOG4586, VOG0704, VOG0539, VOG4589, VOG0204, VOG5361, VOG4553, VOG4568, VOG4556, VOG3644, VOG4544, VOG4581, VOG7403, VOG4841, VOG0198, VOG7372, VOG2752, VOG4606, VOG1227, VOG1936, VOG0535, VOG0650 |
| FFCFBHGA_48 | 4690 | 15220 | True | Siphoviridae | VOG6587, VOG0189, VOG4566, VOG7581, VOG9667, VOG9667, VOG1563, VOG0275, VOG0275 |
| FFCFBHGA_65 | 5821 | 32504 | False | Myoviridae | VOG9667, VOG3490, VOG0189, VOG4843, VOG5447, VOG0796, VOG10227, VOG4773, VOG4685, VOG0749, VOG3890, VOG3891, VOG3892, VOG6163, VOG4865, VOG4885, VOG3894, VOG4550, VOG4691, VOG4862, VOG7718, VOG4705 |
Phigaro v2.4.0 (Database: Jan. 2024 release)