safety

PPRS ARGs VFs PGs Plasmid Prophage IS elements

ARGs	VFs	PGs	PPRS
13	10	8	18.25

PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).

Comprehensive Antibiotic Resistance Database (CARD)

Query ID	Begin	End	ARO name	ARO accession	CARD name	Identity	Coverage	Accession no.
AAKNCBCF_3	455809	457011	blt	ARO:3003006	blt	100.00	100.00	L32599.1
AAKNCBCF_3	437448	438302	aadK	ARO:3002627	aadK	100.00	100.00	AL009126.1
AAKNCBCF_2	425742	426059	ykkD	ARO:3003064	ykkD	100.00	100.00	AL009126.1
AAKNCBCF_2	425404	425742	ykkC	ARO:3003063	ykkC	100.00	100.00	AL009126.1
AAKNCBCF_4	358837	359757	mphK	ARO:3004541	mphK	100.00	100.00	CP010314.1
AAKNCBCF_4	345503	346942	lmrB	ARO:3002813	lmrB	99.79	100.42	JYFL01000006.1
AAKNCBCF_4	295846	296439	tmrB	ARO:3003059	tmrB	100.00	100.00	AL009126.3
AAKNCBCF_10	188073	189242	bmr	ARO:3003007	bmr	99.74	100.00	M33768.1
AAKNCBCF_4	29216	30859	vmlR	ARO:3004476	vmlR	100.00	100.00	NC_000964.3
AAKNCBCF_6	27269	29839	Bacillus subtilis mprF	ARO:3003324	Bsub_mprF	100.00	100.00	AL009126.3

RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)

ResFinder

Query ID	Begin	End	Resistance gene	Phenotype	Identity	Coverage	Accession no.
AAKNCBCF_3	437448	438302	aadK	Streptomycin	100.00	100.00	M26879
AAKNCBCF_4	358837	359757	mph(K)	Spiramycin, Telithromycin	100.00	100.00	NC_000964
AAKNCBCF_5	724902	726278	tet(L)	Doxycycline, Tetracycline, Doxycycline, Tetracycline, Minocycline, Tigecycline	100.00	100.00	X08034

ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)

AMRFinderPlus

Query ID	Begin	End	Gene symbol	Sequence name	Method	Identity	Coverage	Accession no.
AAKNCBCF_3	437448	438299	aadK	aminoglycoside 6-adenylyltransferase AadK	EXACTX	100.00	100.00	WP_003229862.1
AAKNCBCF_4	358840	359757	mphK	macrolide 2'-phosphotransferase MphK	EXACTX	100.00	100.00	WP_003246254.1
AAKNCBCF_5	722384	722902	satA	streptothricin N-acetyltransferase SatA	EXACTX	100.00	100.00	WP_003242546.1
AAKNCBCF_5	724905	726278	tet(L)	tetracycline efflux MFS transporter Tet(L)	EXACTX	100.00	100.00	WP_003242953.1
AAKNCBCF_4	29216	30856	vmlR	ABC-F type ribosomal protection protein VmlR	EXACTX	100.00	100.00	WP_003234144.1
AAKNCBCF_17	71811	74405	rphC	rifamycin-inactivating phosphotransferase RphC	BLASTX	81.15	99.88	WP_087347987.1

AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)

Virulence Factor Database (VFDB)

Query ID	qstart	qend	Gene name	VF name	VF category	Identity	Coverage	Accession no.
AAKNCBCF_5	233759	234901	capA	Capsule	Immune modulation	100.00	100.00	NP_391469
AAKNCBCF_5	235384	236565	capB	Capsule	Immune modulation	100.00	100.00	NP_391471
AAKNCBCF_5	234920	235369	capC	Capsule	Immune modulation	100.00	100.00	NP_391470
AAKNCBCF_17	25153	26916	dep/capD	Capsule	Immune modulation	100.00	100.00	NP_389723
AAKNCBCF_9	112869	113654	dhbA	Bacillibactin	Nutritional/Metabolic factor	100.00	100.00	NP_391080
AAKNCBCF_9	109033	109971	dhbB	Bacillibactin	Nutritional/Metabolic factor	100.00	100.00	NP_391077
AAKNCBCF_9	111647	112843	dhbC	Bacillibactin	Nutritional/Metabolic factor	100.00	100.00	NP_391079
AAKNCBCF_9	109999	111618	dhbE	Bacillibactin	Nutritional/Metabolic factor	100.00	100.00	NP_391078
AAKNCBCF_9	101877	109013	dhbF	Bacillibactin	Nutritional/Metabolic factor	100.00	100.00	NP_391076
AAKNCBCF_8	18096	18737	hlyIII	Hemolysin III	Exotoxin	100.00	100.00	NP_390062

BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)

VirulenceFinder

Query ID	Begin	End	Database	Virulence factor	Protein function	Identity	Coverage	Accession no.
No hit found

VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)

PHI-base

Query ID	qstart	qend	Gene name	e-value	Function	Identity	Coverage	Gene ID
AAKNCBCF_16	27995	26859	tufA	0.0	elongation factor Tu	84.96	95.69	AHM69299
AAKNCBCF_14	4269	5138	pdxS	1.12e-151	encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS	83.79	98.31	ACE61268
AAKNCBCF_2	738979	739755	CodY	7.54e-125	nutrient-responsive regulator	81.08	100.00	ACJ80679
AAKNCBCF_5	82172	82741	ClpP	4.58e-99	part of proteolytic complex	81.05	97.44	BAB94595
AAKNCBCF_2	276581	276973	spxA1	2.23e-69	redox-responsive transcription factor	83.21	100.00	AAF21893
AAKNCBCF_13	34595	34353	SpoVG	2.73e-38	RNA-binding protein	80.25	79.41	AAT02994
AAKNCBCF_4	76331	76134	CspA	1.98e-31	cold shock protein	90.91	100.00	CAA62903
AAKNCBCF_10	1313	1507	CspD	9.20e-28	cold shock protein	80.00	98.48	CAC99957
AAKNCBCF_10	1313	1510	CspB	1.91e-27	cold shock protein	80.30	100.00	CAD00094

BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)

PlasmidFinder

Query ID	Begin	End	Plasmid	Query / Template	Identity	Accession no.
No hit found

PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)

PlasmidHunter

Query ID	Prediction	Chromosome	Plasmid
AAKNCBCF_2	0.0	1.0	0.0
AAKNCBCF_3	0.0	1.0	0.0
AAKNCBCF_4	0.0	1.0	0.0
AAKNCBCF_5	0.0	1.0	0.0
AAKNCBCF_6	0.0	1.0	0.0
AAKNCBCF_8	0.0	1.0	0.0
AAKNCBCF_9	0.0	1.0	0.0
AAKNCBCF_10	0.0	1.0	0.0
AAKNCBCF_11	1.0	0.0	1.0
AAKNCBCF_12	0.0	1.0	0.0
AAKNCBCF_13	0.0	1.0	0.0
AAKNCBCF_14	0.0	1.0	0.0
AAKNCBCF_15	0.0	1.0	0.0
AAKNCBCF_16	0.0	1.0	0.0
AAKNCBCF_17	0.0	1.0	0.0
AAKNCBCF_18	0.0	1.0	0.0
AAKNCBCF_19	0.0	1.0	0.0
AAKNCBCF_21	0.0	1.0	0.0
AAKNCBCF_22	0.0	1.0	0.0
AAKNCBCF_24	0.0	1.0	0.0
AAKNCBCF_25	0.0	1.0	0.0
AAKNCBCF_26	0.0	1.0	0.0
AAKNCBCF_27	0.0	1.0	0.0
AAKNCBCF_28	0.0	1.0	0.0
AAKNCBCF_29	1.0	0.0	1.0
AAKNCBCF_31	0.0	1.0	0.0
AAKNCBCF_32	1.0	0.0	1.0
AAKNCBCF_34	0.0	1.0	0.0

PlasmidHunter v 1.4.5 (Database: May. 2024 release)

Phigaro

Query ID	Begin	End	Transposable	Taxonomy	pVOGs
AAKNCBCF_2	369457	395888	False	Myoviridae	VOG4602, VOG9667, VOG10994, VOG0189, VOG4861, VOG5447, VOG0796, VOG10227, VOG4773, VOG4685, VOG0749, VOG3890, VOG3891, VOG3892, VOG6163, VOG4865, VOG4885, VOG3894, VOG4550, VOG4691, VOG4862, VOG7718, VOG4705
AAKNCBCF_15	2085	20169	False	Myoviridae	VOG7718, VOG4862, VOG4691, VOG4550, VOG3894, VOG4885, VOG4865, VOG6163, VOG3892, VOG3891, VOG3890, VOG10055, VOG4870, VOG4589, VOG0749

Phigaro v2.4.0 (Database: Jan. 2024 release)