Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 12 | 10 | 8 | 17.55 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| MCCBFOHN_7 | 342953 | 343873 | mphK | ARO:3004541 | mphK | 98.68 | 100.00 | CP010314.1 |
| MCCBFOHN_7 | 329620 | 331059 | lmrB | ARO:3002813 | lmrB | 99.16 | 100.42 | JYFL01000006.1 |
| MCCBFOHN_7 | 280167 | 280760 | tmrB | ARO:3003059 | tmrB | 98.98 | 100.00 | AL009126.3 |
| MCCBFOHN_15 | 176308 | 176646 | ykkC | ARO:3003063 | ykkC | 100.00 | 100.00 | AL009126.1 |
| MCCBFOHN_15 | 175991 | 176308 | ykkD | ARO:3003064 | ykkD | 99.05 | 100.00 | AL009126.1 |
| MCCBFOHN_18 | 170731 | 171900 | bmr | ARO:3003007 | bmr | 99.74 | 100.00 | M33768.1 |
| MCCBFOHN_22 | 165973 | 168543 | Bacillus subtilis mprF | ARO:3003324 | Bsub_mprF | 99.77 | 100.00 | AL009126.3 |
| MCCBFOHN_7 | 28898 | 30544 | vmlR | ARO:3004476 | vmlR | 98.72 | 100.18 | NC_000964.3 |
| MCCBFOHN_10 | 23596 | 24444 | aadK | ARO:3002627 | aadK | 99.25 | 99.30 | AL009126.1 |
| MCCBFOHN_10 | 6463 | 7665 | blt | ARO:3003006 | blt | 99.25 | 100.00 | L32599.1 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| MCCBFOHN_10 | 23591 | 24444 | aadK | Streptomycin | 99.06 | 99.88 | M26879 |
| MCCBFOHN_7 | 342953 | 343873 | mph(K) | Spiramycin, Telithromycin | 98.91 | 100.00 | NC_000964 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| MCCBFOHN_10 | 23647 | 24444 | aadK | aminoglycoside 6-adenylyltransferase AadK | BLASTX | 99.25 | 93.66 | WP_003229862.1 |
| MCCBFOHN_7 | 28898 | 30541 | vmlR | ABC-F type ribosomal protection protein VmlR | BLASTX | 98.72 | 100.00 | WP_003234144.1 |
| MCCBFOHN_7 | 342965 | 343873 | mphK | macrolide 2'-phosphotransferase MphK | BLASTX | 98.68 | 99.02 | WP_003246254.1 |
| MCCBFOHN_3 | 38306 | 38824 | satA | streptothricin N-acetyltransferase SatA | BLASTX | 93.06 | 100.00 | WP_003242546.1 |
| MCCBFOHN_45 | 5692 | 8289 | rphC | rifamycin-inactivating phosphotransferase RphC | BLASTX | 80.83 | 100.00 | WP_087347987.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| MCCBFOHN_8 | 216250 | 217035 | dhbA | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.87 | 100.00 | NP_391080 |
| MCCBFOHN_21 | 95709 | 96350 | hlyIII | 0.0 | Hemolysin III | Exotoxin | 99.84 | 100.00 | NP_390062 |
| MCCBFOHN_16 | 3229 | 4410 | capB | 0.0 | Capsule | Immune modulation | 99.58 | 100.00 | NP_391471 |
| MCCBFOHN_8 | 217061 | 218257 | dhbC | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.33 | 100.00 | NP_391079 |
| MCCBFOHN_16 | 2765 | 3214 | capC | 0.0 | Capsule | Immune modulation | 99.11 | 100.00 | NP_391470 |
| MCCBFOHN_16 | 1604 | 2746 | capA | 0.0 | Capsule | Immune modulation | 99.04 | 100.00 | NP_391469 |
| MCCBFOHN_8 | 219933 | 220871 | dhbB | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.04 | 100.00 | NP_391077 |
| MCCBFOHN_12 | 10173 | 11936 | dep/capD | 0.0 | Capsule | Immune modulation | 98.92 | 100.00 | NP_389723 |
| MCCBFOHN_8 | 220891 | 228027 | dhbF | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.56 | 100.00 | NP_391076 |
| MCCBFOHN_8 | 218286 | 219905 | dhbE | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 97.16 | 100.00 | NP_391078 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| MCCBFOHN_39 | 27992 | 26856 | tufA | 0.0 | elongation factor Tu | 84.96 | 95.69 | AHM69299 |
| MCCBFOHN_30 | 4501 | 5370 | pdxS | 1.12e-151 | encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS | 83.79 | 98.31 | ACE61268 |
| MCCBFOHN_20 | 132475 | 133251 | CodY | 1.90e-125 | nutrient-responsive regulator | 81.08 | 100.00 | ACJ80679 |
| MCCBFOHN_19 | 89331 | 88762 | ClpP | 8.20e-100 | part of proteolytic complex | 81.05 | 97.44 | BAB94595 |
| MCCBFOHN_13 | 40009 | 40401 | spxA1 | 4.91e-71 | redox-responsive transcription factor | 83.97 | 100.00 | AAF21893 |
| MCCBFOHN_13 | 40009 | 40401 | spx | 2.93e-69 | essential gene which is a thiol/oxidative stress sensor | 80.15 | 100.00 | BAB57159 |
| MCCBFOHN_2 | 34704 | 34462 | SpoVG | 7.53e-39 | RNA-binding protein | 81.48 | 79.41 | AAT02994 |
| MCCBFOHN_7 | 76901 | 76704 | CspA | 1.85e-31 | cold shock protein | 90.91 | 100.00 | CAA62903 |
| MCCBFOHN_21 | 107581 | 107775 | CspD | 4.93e-28 | cold shock protein | 80.00 | 98.48 | CAC99957 |
| MCCBFOHN_21 | 107581 | 107778 | CspB | 1.02e-27 | cold shock protein | 80.30 | 100.00 | CAD00094 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| MCCBFOHN_2 | 0.0 | 1.0 | 0.0 |
| MCCBFOHN_3 | 0.0 | 1.0 | 0.0 |
| MCCBFOHN_4 | 0.0 | 1.0 | 0.0 |
| MCCBFOHN_5 | 0.0 | 1.0 | 0.0 |
| MCCBFOHN_6 | 0.0 | 1.0 | 0.0 |
| MCCBFOHN_7 | 0.0 | 1.0 | 0.0 |
| MCCBFOHN_8 | 0.0 | 1.0 | 0.0 |
| MCCBFOHN_9 | 0.0 | 1.0 | 0.0 |
| MCCBFOHN_10 | 0.0 | 1.0 | 0.0 |
| MCCBFOHN_11 | 0.0 | 1.0 | 0.0 |
| MCCBFOHN_12 | 0.0 | 1.0 | 0.0 |
| MCCBFOHN_13 | 0.0 | 1.0 | 0.0 |
| MCCBFOHN_14 | 0.0 | 1.0 | 0.0 |
| MCCBFOHN_15 | 0.0 | 1.0 | 0.0 |
| MCCBFOHN_16 | 0.0 | 1.0 | 0.0 |
| MCCBFOHN_17 | 0.0 | 1.0 | 0.0 |
| MCCBFOHN_18 | 0.0 | 1.0 | 0.0 |
| MCCBFOHN_19 | 0.0 | 1.0 | 0.0 |
| MCCBFOHN_20 | 0.0 | 1.0 | 0.0 |
| MCCBFOHN_21 | 0.0 | 1.0 | 0.0 |
| MCCBFOHN_22 | 0.0 | 1.0 | 0.0 |
| MCCBFOHN_23 | 0.0 | 1.0 | 0.0 |
| MCCBFOHN_24 | 0.0 | 1.0 | 0.0 |
| MCCBFOHN_25 | 0.0 | 1.0 | 0.0 |
| MCCBFOHN_26 | 0.0 | 1.0 | 0.0 |
| MCCBFOHN_28 | 0.0 | 1.0 | 0.0 |
| MCCBFOHN_29 | 0.0 | 1.0 | 0.0 |
| MCCBFOHN_30 | 0.0 | 1.0 | 0.0 |
| MCCBFOHN_31 | 0.0 | 1.0 | 0.0 |
| MCCBFOHN_33 | 0.0 | 1.0 | 0.0 |
| MCCBFOHN_34 | 0.0 | 1.0 | 0.0 |
| MCCBFOHN_36 | 0.0 | 1.0 | 0.0 |
| MCCBFOHN_37 | 0.0 | 1.0 | 0.0 |
| MCCBFOHN_38 | 0.0 | 1.0 | 0.0 |
| MCCBFOHN_39 | 0.0 | 1.0 | 0.0 |
| MCCBFOHN_40 | 0.0 | 1.0 | 0.0 |
| MCCBFOHN_41 | 0.0 | 1.0 | 0.0 |
| MCCBFOHN_44 | 0.0 | 1.0 | 0.0 |
| MCCBFOHN_45 | 0.0 | 1.0 | 0.0 |
| MCCBFOHN_46 | 0.0 | 1.0 | 0.0 |
| MCCBFOHN_47 | 0.0 | 1.0 | 0.0 |
| MCCBFOHN_48 | 0.0 | 1.0 | 0.0 |
| MCCBFOHN_49 | 1.0 | 0.0 | 1.0 |
| MCCBFOHN_50 | 0.0 | 1.0 | 0.0 |
| MCCBFOHN_52 | 0.0 | 1.0 | 0.0 |
| MCCBFOHN_53 | 0.0 | 1.0 | 0.0 |
| MCCBFOHN_54 | 0.0 | 1.0 | 0.0 |
| MCCBFOHN_55 | 0.0 | 1.0 | 0.0 |
| MCCBFOHN_57 | 0.0 | 1.0 | 0.0 |
| MCCBFOHN_58 | 0.0 | 1.0 | 0.0 |
| MCCBFOHN_59 | 0.0 | 1.0 | 0.0 |
| MCCBFOHN_60 | 0.0 | 1.0 | 0.0 |
| MCCBFOHN_61 | 0.0 | 1.0 | 0.0 |
| MCCBFOHN_64 | 0.0 | 1.0 | 0.0 |
| MCCBFOHN_65 | 0.0 | 1.0 | 0.0 |
| MCCBFOHN_69 | 0.0 | 1.0 | 0.0 |
| MCCBFOHN_70 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| MCCBFOHN_15 | 205888 | 230429 | False | Myoviridae | VOG0136, VOG0753, VOG4705, VOG3390, VOG7718, VOG4862, VOG4691, VOG4550, VOG3894, VOG4885, VOG4865, VOG6163, VOG3892, VOG3891, VOG3890, VOG0749, VOG4685, VOG4773, VOG10227, VOG0796, VOG5447, VOG4861, VOG0189, VOG3490 |
Phigaro v2.4.0 (Database: Jan. 2024 release)