Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 4 | 2 | 6 | 7.48 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| BNGFGAPD_30 | 586 | 1632 | clbA | ARO:3002814 | clbA | 92.77 | 99.71 | CP006845.1 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| BNGFGAPD_30 | 586 | 1629 | cfr(B) | Chloramphenicol, Florfenicol, Clindamycin, Lincomycin, Linezolid, Dalfopristin, Pristinamycin IIA, Virginiamycin M, Tiamulin | 88.90 | 99.33 | KR610408 |
| BNGFGAPD_81 | 358 | 1734 | tet(L) | Doxycycline, Tetracycline, Doxycycline, Tetracycline, Minocycline, Tigecycline | 87.15 | 100.00 | X08034 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| BNGFGAPD_30 | 586 | 1629 | clbA | 23S rRNA (adenine(2503)-C(8))-methyltransferase ClbA | BLASTX | 92.24 | 99.71 | WP_012116915.1 |
| BNGFGAPD_81 | 358 | 1728 | tet(L) | tetracycline efflux MFS transporter Tet(L) | BLASTX | 86.43 | 99.78 | WP_003242953.1 |
| BNGFGAPD_135 | 774 | 1265 | satA | streptothricin N-acetyltransferase SatA | BLASTX | 84.15 | 94.80 | WP_003242546.1 |
| BNGFGAPD_142 | 1 | 2133 | rphC | rifamycin-inactivating phosphotransferase RphC | PARTIAL_CONTIG_ENDX | 80.59 | 82.14 | WP_087347987.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| BNGFGAPD_16 | 77589 | 78770 | capB | 0.0 | Capsule | Immune modulation | 81.79 | 99.66 | NP_391471 |
| BNGFGAPD_16 | 78785 | 79234 | capC | 3.65e-115 | Capsule | Immune modulation | 83.59 | 99.78 | NP_391470 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| BNGFGAPD_112 | 3620 | 4756 | tufA | 0.0 | elongation factor Tu | 86.54 | 95.69 | AHM69299 |
| BNGFGAPD_50 | 1593 | 724 | pdxS | 1.59e-154 | encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS | 84.83 | 98.31 | ACE61268 |
| BNGFGAPD_53 | 25644 | 24868 | CodY | 2.22e-126 | nutrient-responsive regulator | 81.08 | 100.00 | ACJ80679 |
| BNGFGAPD_29 | 39080 | 38688 | spxA1 | 6.02e-71 | redox-responsive transcription factor | 83.21 | 100.00 | AAF21893 |
| BNGFGAPD_99 | 674 | 477 | CspA | 5.95e-34 | cold shock protein | 92.42 | 100.00 | CAA62903 |
| BNGFGAPD_99 | 674 | 480 | CspR | 2.95e-28 | cold shock protein | 80.00 | 98.48 | AAO82613 |
| BNGFGAPD_11 | 13773 | 13579 | CspB | 4.58e-27 | cold shock protein | 84.61 | 98.48 | CAD00094 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| BNGFGAPD_1 | 0.0 | 1.0 | 0.0 |
| BNGFGAPD_2 | 0.0 | 1.0 | 0.0 |
| BNGFGAPD_3 | 0.0 | 1.0 | 0.0 |
| BNGFGAPD_4 | 0.0 | 1.0 | 0.0 |
| BNGFGAPD_5 | 0.0 | 1.0 | 0.0 |
| BNGFGAPD_7 | 0.0 | 1.0 | 0.0 |
| BNGFGAPD_8 | 0.0 | 1.0 | 0.0 |
| BNGFGAPD_9 | 0.0 | 1.0 | 0.0 |
| BNGFGAPD_10 | 0.0 | 1.0 | 0.0 |
| BNGFGAPD_11 | 0.0 | 1.0 | 0.0 |
| BNGFGAPD_12 | 0.0 | 1.0 | 0.0 |
| BNGFGAPD_13 | 0.0 | 1.0 | 0.0 |
| BNGFGAPD_14 | 0.0 | 1.0 | 0.0 |
| BNGFGAPD_15 | 0.0 | 1.0 | 0.0 |
| BNGFGAPD_16 | 0.0 | 1.0 | 0.0 |
| BNGFGAPD_17 | 0.0 | 1.0 | 0.0 |
| BNGFGAPD_18 | 0.0 | 1.0 | 0.0 |
| BNGFGAPD_19 | 0.0 | 1.0 | 0.0 |
| BNGFGAPD_20 | 0.0 | 1.0 | 0.0 |
| BNGFGAPD_21 | 0.0 | 1.0 | 0.0 |
| BNGFGAPD_22 | 0.0 | 1.0 | 0.0 |
| BNGFGAPD_23 | 1.0 | 0.0 | 1.0 |
| BNGFGAPD_24 | 0.0 | 1.0 | 0.0 |
| BNGFGAPD_25 | 0.0 | 1.0 | 0.0 |
| BNGFGAPD_26 | 0.0 | 1.0 | 0.0 |
| BNGFGAPD_27 | 0.0 | 1.0 | 0.0 |
| BNGFGAPD_28 | 0.0 | 1.0 | 0.0 |
| BNGFGAPD_29 | 0.0 | 1.0 | 0.0 |
| BNGFGAPD_30 | 0.0 | 1.0 | 0.0 |
| BNGFGAPD_31 | 0.0 | 1.0 | 0.0 |
| BNGFGAPD_32 | 0.0 | 1.0 | 0.0 |
| BNGFGAPD_33 | 0.0 | 1.0 | 0.0 |
| BNGFGAPD_34 | 0.0 | 1.0 | 0.0 |
| BNGFGAPD_35 | 0.0 | 1.0 | 0.0 |
| BNGFGAPD_36 | 0.0 | 1.0 | 0.0 |
| BNGFGAPD_37 | 0.0 | 1.0 | 0.0 |
| BNGFGAPD_38 | 0.0 | 1.0 | 0.0 |
| BNGFGAPD_39 | 0.0 | 1.0 | 0.0 |
| BNGFGAPD_40 | 0.0 | 1.0 | 0.0 |
| BNGFGAPD_41 | 0.0 | 1.0 | 0.0 |
| BNGFGAPD_42 | 0.0 | 1.0 | 0.0 |
| BNGFGAPD_43 | 0.0 | 1.0 | 0.0 |
| BNGFGAPD_44 | 0.0 | 1.0 | 0.0 |
| BNGFGAPD_46 | 0.0 | 1.0 | 0.0 |
| BNGFGAPD_47 | 0.0 | 1.0 | 0.0 |
| BNGFGAPD_48 | 0.0 | 1.0 | 0.0 |
| BNGFGAPD_49 | 0.0 | 1.0 | 0.0 |
| BNGFGAPD_50 | 0.0 | 1.0 | 0.0 |
| BNGFGAPD_51 | 0.0 | 1.0 | 0.0 |
| BNGFGAPD_52 | 0.0 | 1.0 | 0.0 |
| BNGFGAPD_53 | 0.0 | 1.0 | 0.0 |
| BNGFGAPD_54 | 0.0 | 1.0 | 0.0 |
| BNGFGAPD_55 | 0.0 | 1.0 | 0.0 |
| BNGFGAPD_56 | 0.0 | 1.0 | 0.0 |
| BNGFGAPD_57 | 0.0 | 1.0 | 0.0 |
| BNGFGAPD_58 | 0.0 | 1.0 | 0.0 |
| BNGFGAPD_59 | 0.0 | 1.0 | 0.0 |
| BNGFGAPD_60 | 1.0 | 0.0 | 1.0 |
| BNGFGAPD_61 | 0.0 | 1.0 | 0.0 |
| BNGFGAPD_62 | 0.0 | 1.0 | 0.0 |
| BNGFGAPD_63 | 0.0 | 1.0 | 0.0 |
| BNGFGAPD_64 | 0.0 | 1.0 | 0.0 |
| BNGFGAPD_65 | 0.0 | 1.0 | 0.0 |
| BNGFGAPD_66 | 0.0 | 1.0 | 0.0 |
| BNGFGAPD_67 | 1.0 | 0.0 | 1.0 |
| BNGFGAPD_68 | 0.0 | 1.0 | 0.0 |
| BNGFGAPD_69 | 0.0 | 1.0 | 0.0 |
| BNGFGAPD_70 | 0.0 | 1.0 | 0.0 |
| BNGFGAPD_71 | 0.0 | 1.0 | 0.0 |
| BNGFGAPD_72 | 0.0 | 1.0 | 0.0 |
| BNGFGAPD_73 | 0.0 | 1.0 | 0.0 |
| BNGFGAPD_74 | 0.0 | 1.0 | 0.0 |
| BNGFGAPD_75 | 0.0 | 1.0 | 0.0 |
| BNGFGAPD_77 | 0.0 | 1.0 | 0.0 |
| BNGFGAPD_78 | 0.0 | 1.0 | 0.0 |
| BNGFGAPD_79 | 0.0 | 1.0 | 0.0 |
| BNGFGAPD_80 | 0.0 | 1.0 | 0.0 |
| BNGFGAPD_81 | 0.0 | 1.0 | 0.0 |
| BNGFGAPD_82 | 0.0 | 1.0 | 0.0 |
| BNGFGAPD_83 | 0.0 | 1.0 | 0.0 |
| BNGFGAPD_84 | 0.0 | 1.0 | 0.0 |
| BNGFGAPD_85 | 0.0 | 1.0 | 0.0 |
| BNGFGAPD_86 | 0.0 | 1.0 | 0.0 |
| BNGFGAPD_87 | 0.0 | 1.0 | 0.0 |
| BNGFGAPD_88 | 1.0 | 0.0 | 1.0 |
| BNGFGAPD_89 | 0.0 | 1.0 | 0.0 |
| BNGFGAPD_91 | 0.0 | 1.0 | 0.0 |
| BNGFGAPD_93 | 0.0 | 1.0 | 0.0 |
| BNGFGAPD_94 | 0.0 | 1.0 | 0.0 |
| BNGFGAPD_95 | 0.0 | 1.0 | 0.0 |
| BNGFGAPD_96 | 0.0 | 1.0 | 0.0 |
| BNGFGAPD_97 | 0.0 | 1.0 | 0.0 |
| BNGFGAPD_98 | 1.0 | 0.0 | 1.0 |
| BNGFGAPD_99 | 1.0 | 0.0 | 1.0 |
| BNGFGAPD_100 | 0.0 | 1.0 | 0.0 |
| BNGFGAPD_101 | 0.0 | 1.0 | 0.0 |
| BNGFGAPD_102 | 1.0 | 0.0 | 1.0 |
| BNGFGAPD_103 | 0.0 | 1.0 | 0.0 |
| BNGFGAPD_104 | 1.0 | 0.0 | 1.0 |
| BNGFGAPD_105 | 0.0 | 1.0 | 0.0 |
| BNGFGAPD_106 | 0.0 | 1.0 | 0.0 |
| BNGFGAPD_107 | 0.0 | 1.0 | 0.0 |
| BNGFGAPD_108 | 0.0 | 1.0 | 0.0 |
| BNGFGAPD_110 | 0.0 | 1.0 | 0.0 |
| BNGFGAPD_111 | 0.0 | 1.0 | 0.0 |
| BNGFGAPD_112 | 0.0 | 1.0 | 0.0 |
| BNGFGAPD_113 | 0.0 | 1.0 | 0.0 |
| BNGFGAPD_114 | 0.0 | 1.0 | 0.0 |
| BNGFGAPD_115 | 1.0 | 0.0 | 1.0 |
| BNGFGAPD_116 | 0.0 | 1.0 | 0.0 |
| BNGFGAPD_118 | 0.0 | 1.0 | 0.0 |
| BNGFGAPD_120 | 1.0 | 0.0 | 1.0 |
| BNGFGAPD_121 | 0.0 | 1.0 | 0.0 |
| BNGFGAPD_122 | 0.0 | 1.0 | 0.0 |
| BNGFGAPD_123 | 0.0 | 1.0 | 0.0 |
| BNGFGAPD_124 | 0.0 | 1.0 | 0.0 |
| BNGFGAPD_125 | 0.0 | 1.0 | 0.0 |
| BNGFGAPD_126 | 0.0 | 1.0 | 0.0 |
| BNGFGAPD_127 | 0.0 | 1.0 | 0.0 |
| BNGFGAPD_128 | 0.0 | 1.0 | 0.0 |
| BNGFGAPD_129 | 0.0 | 1.0 | 0.0 |
| BNGFGAPD_130 | 0.0 | 1.0 | 0.0 |
| BNGFGAPD_131 | 1.0 | 0.0 | 1.0 |
| BNGFGAPD_132 | 0.0 | 1.0 | 0.0 |
| BNGFGAPD_133 | 0.0 | 1.0 | 0.0 |
| BNGFGAPD_134 | 0.0 | 1.0 | 0.0 |
| BNGFGAPD_135 | 0.0 | 1.0 | 0.0 |
| BNGFGAPD_136 | 0.0 | 1.0 | 0.0 |
| BNGFGAPD_137 | 0.0 | 1.0 | 0.0 |
| BNGFGAPD_139 | 1.0 | 0.0 | 1.0 |
| BNGFGAPD_140 | 0.0 | 1.0 | 0.0 |
| BNGFGAPD_141 | 0.0 | 1.0 | 0.0 |
| BNGFGAPD_142 | 1.0 | 0.0 | 1.0 |
| BNGFGAPD_143 | 0.0 | 1.0 | 0.0 |
| BNGFGAPD_144 | 1.0 | 0.0 | 1.0 |
| BNGFGAPD_145 | 0.0 | 1.0 | 0.0 |
| BNGFGAPD_146 | 0.0 | 1.0 | 0.0 |
| BNGFGAPD_153 | 0.0 | 1.0 | 0.0 |
| BNGFGAPD_154 | 0.0 | 1.0 | 0.0 |
| BNGFGAPD_155 | 0.0 | 1.0 | 0.0 |
| BNGFGAPD_156 | 0.0 | 1.0 | 0.0 |
| BNGFGAPD_157 | 0.0 | 1.0 | 0.0 |
| BNGFGAPD_158 | 1.0 | 0.0 | 1.0 |
| BNGFGAPD_159 | 0.0 | 1.0 | 0.0 |
| BNGFGAPD_160 | 0.0 | 1.0 | 0.0 |
| BNGFGAPD_161 | 0.0 | 1.0 | 0.0 |
| BNGFGAPD_163 | 0.0 | 1.0 | 0.0 |
| BNGFGAPD_164 | 0.0 | 1.0 | 0.0 |
| BNGFGAPD_165 | 0.0 | 1.0 | 0.0 |
| BNGFGAPD_166 | 1.0 | 0.0 | 1.0 |
| BNGFGAPD_167 | 0.0 | 1.0 | 0.0 |
| BNGFGAPD_168 | 1.0 | 0.0 | 1.0 |
| BNGFGAPD_169 | 0.0 | 1.0 | 0.0 |
| BNGFGAPD_170 | 1.0 | 0.0 | 1.0 |
| BNGFGAPD_171 | 1.0 | 0.0 | 1.0 |
| BNGFGAPD_172 | 0.0 | 1.0 | 0.0 |
| BNGFGAPD_173 | 0.0 | 1.0 | 0.0 |
| BNGFGAPD_174 | 0.0 | 1.0 | 0.0 |
| BNGFGAPD_176 | 0.0 | 1.0 | 0.0 |
| BNGFGAPD_177 | 1.0 | 0.0 | 1.0 |
| BNGFGAPD_178 | 0.0 | 1.0 | 0.0 |
| BNGFGAPD_179 | 0.0 | 1.0 | 0.0 |
| BNGFGAPD_180 | 0.0 | 1.0 | 0.0 |
| BNGFGAPD_182 | 0.0 | 1.0 | 0.0 |
| BNGFGAPD_183 | 0.0 | 1.0 | 0.0 |
| BNGFGAPD_184 | 0.0 | 1.0 | 0.0 |
| BNGFGAPD_185 | 0.0 | 1.0 | 0.0 |
| BNGFGAPD_186 | 0.0 | 1.0 | 0.0 |
| BNGFGAPD_187 | 0.0 | 1.0 | 0.0 |
| BNGFGAPD_189 | 0.0 | 1.0 | 0.0 |
| BNGFGAPD_190 | 0.0 | 1.0 | 0.0 |
| BNGFGAPD_191 | 0.0 | 1.0 | 0.0 |
| BNGFGAPD_193 | 0.0 | 1.0 | 0.0 |
| BNGFGAPD_194 | 0.0 | 1.0 | 0.0 |
| BNGFGAPD_196 | 0.0 | 1.0 | 0.0 |
| BNGFGAPD_197 | 0.0 | 1.0 | 0.0 |
| BNGFGAPD_198 | 1.0 | 0.0 | 1.0 |
| BNGFGAPD_199 | 0.0 | 1.0 | 0.0 |
| BNGFGAPD_201 | 0.0 | 1.0 | 0.0 |
| BNGFGAPD_202 | 0.0 | 1.0 | 0.0 |
| BNGFGAPD_204 | 0.0 | 1.0 | 0.0 |
| BNGFGAPD_206 | 0.0 | 1.0 | 0.0 |
| BNGFGAPD_207 | 1.0 | 0.0 | 1.0 |
| BNGFGAPD_208 | 1.0 | 0.0 | 1.0 |
| BNGFGAPD_209 | 1.0 | 0.0 | 1.0 |
| BNGFGAPD_210 | 0.0 | 1.0 | 0.0 |
| BNGFGAPD_214 | 0.0 | 1.0 | 0.0 |
| BNGFGAPD_216 | 1.0 | 0.0 | 1.0 |
| BNGFGAPD_217 | 0.0 | 1.0 | 0.0 |
| BNGFGAPD_219 | 0.0 | 1.0 | 0.0 |
| BNGFGAPD_221 | 0.0 | 1.0 | 0.0 |
| BNGFGAPD_223 | 0.0 | 1.0 | 0.0 |
| BNGFGAPD_225 | 0.0 | 1.0 | 0.0 |
| BNGFGAPD_226 | 1.0 | 0.0 | 1.0 |
| BNGFGAPD_230 | 0.0 | 1.0 | 0.0 |
| BNGFGAPD_234 | 1.0 | 0.0 | 1.0 |
| BNGFGAPD_243 | 1.0 | 0.0 | 1.0 |
| BNGFGAPD_245 | 1.0 | 0.0 | 1.0 |
| BNGFGAPD_251 | 1.0 | 0.0 | 1.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| BNGFGAPD_1 | 127786 | 136860 | False | Siphoviridae | VOG0181, VOG7438, VOG1047, VOG0021, VOG1565, VOG0026, VOG10500 |
| BNGFGAPD_3 | 26197 | 54559 | False | Siphoviridae | VOG0602, VOG4824, VOG5221, VOG4705, VOG3390, VOG4612, VOG4660, VOG4619, VOG0801, VOG0083, VOG11074, VOG2565, VOG0724, VOG5361, VOG3676, VOG4555, VOG0692, VOG2613, VOG3699, VOG5023, VOG0275, VOG1183, VOG5327, VOG6495, VOG7742, VOG7541 |
| BNGFGAPD_3 | 74223 | 77481 | False | Unknown | VOG10564, VOG10937 |
| BNGFGAPD_18 | 47021 | 77348 | False | Siphoviridae | VOG5353, VOG0181, VOG4552, VOG3547, VOG4566, VOG9600, VOG0025, VOG1884, VOG5969, VOG0425, VOG5447, VOG0376, VOG2545, VOG4841, VOG4581, VOG4544, VOG4556, VOG4573, VOG4553, VOG0204, VOG4589, VOG0207, VOG4586, VOG5852, VOG6163, VOG0801, VOG3462, VOG3463, VOG4619, VOG4856, VOG0804, VOG4705, VOG5221 |
| BNGFGAPD_21 | 144284 | 151115 | False | Siphoviridae | VOG1775, VOG4707, VOG0641, VOG7896, VOG3390, VOG4612 |
| BNGFGAPD_37 | 36356 | 56124 | False | Myoviridae | VOG0136, VOG0753, VOG4705, VOG3390, VOG7718, VOG4862, VOG4691, VOG4550, VOG3894, VOG4885, VOG4865, VOG6163, VOG3892, VOG3891, VOG3890, VOG10055, VOG0749 |
| BNGFGAPD_38 | 4726 | 22695 | False | Siphoviridae | VOG0796, VOG4681, VOG4972, VOG5447, VOG1137, VOG1485, VOG0044, VOG0102, VOG6581, VOG0397, VOG8569, VOG4658, VOG1336, VOG0536, VOG0044, VOG0189, VOG9093, VOG1309, VOG4600, VOG4600, VOG4552, VOG0650 |
Phigaro v2.4.0 (Database: Jan. 2024 release)