Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 10 | 10 | 9 | 16.76 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| IABFJGIH_23 | 84757 | 85095 | ykkC | ARO:3003063 | ykkC | 98.21 | 100.00 | AL009126.1 |
| IABFJGIH_23 | 84440 | 84757 | ykkD | ARO:3003064 | ykkD | 98.10 | 100.00 | AL009126.1 |
| IABFJGIH_32 | 61079 | 62281 | blt | ARO:3003006 | blt | 98.00 | 100.00 | L32599.1 |
| IABFJGIH_38 | 59379 | 61949 | Bacillus subtilis mprF | ARO:3003324 | Bsub_mprF | 99.53 | 100.00 | AL009126.3 |
| IABFJGIH_32 | 44330 | 45184 | aadK | ARO:3002627 | aadK | 98.59 | 100.00 | AL009126.1 |
| IABFJGIH_51 | 40862 | 41782 | mphK | ARO:3004541 | mphK | 98.68 | 100.00 | CP010314.1 |
| IABFJGIH_58 | 28568 | 30214 | vmlR | ARO:3004476 | vmlR | 98.36 | 100.18 | NC_000964.3 |
| IABFJGIH_51 | 27528 | 28967 | lmrB | ARO:3002813 | lmrB | 99.58 | 100.42 | JYFL01000006.1 |
| IABFJGIH_47 | 872 | 1465 | tmrB | ARO:3003059 | tmrB | 99.49 | 100.00 | AL009126.3 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| IABFJGIH_32 | 44330 | 45184 | aadK | Streptomycin | 98.83 | 100.00 | M26879 |
| IABFJGIH_51 | 40862 | 41782 | mph(K) | Spiramycin, Telithromycin | 98.05 | 100.00 | NC_000964 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| IABFJGIH_51 | 40874 | 41782 | mphK | macrolide 2'-phosphotransferase MphK | BLASTX | 98.68 | 99.02 | WP_003246254.1 |
| IABFJGIH_32 | 44330 | 45181 | aadK | aminoglycoside 6-adenylyltransferase AadK | BLASTX | 98.59 | 100.00 | WP_003229862.1 |
| IABFJGIH_58 | 28568 | 30211 | vmlR | ABC-F type ribosomal protection protein VmlR | BLASTX | 98.36 | 100.00 | WP_003234144.1 |
| IABFJGIH_25 | 50940 | 51458 | satA | streptothricin N-acetyltransferase SatA | BLASTX | 90.75 | 100.00 | WP_003242546.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| IABFJGIH_15 | 253643 | 254824 | capB | 0.0 | Capsule | Immune modulation | 99.66 | 100.00 | NP_391471 |
| IABFJGIH_4 | 22953 | 23738 | dhbA | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.49 | 100.00 | NP_391080 |
| IABFJGIH_15 | 255307 | 256449 | capA | 0.0 | Capsule | Immune modulation | 99.30 | 100.00 | NP_391469 |
| IABFJGIH_4 | 27594 | 34730 | dhbF | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.30 | 100.00 | NP_391076 |
| IABFJGIH_65 | 1395 | 3158 | dep/capD | 0.0 | Capsule | Immune modulation | 99.04 | 100.00 | NP_389723 |
| IABFJGIH_4 | 26636 | 27574 | dhbB | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.04 | 100.00 | NP_391077 |
| IABFJGIH_4 | 23764 | 24960 | dhbC | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.00 | 100.00 | NP_391079 |
| IABFJGIH_22 | 32475 | 33116 | hlyIII | 0.0 | Hemolysin III | Exotoxin | 98.91 | 100.00 | NP_390062 |
| IABFJGIH_15 | 254839 | 255288 | capC | 0.0 | Capsule | Immune modulation | 98.89 | 100.00 | NP_391470 |
| IABFJGIH_4 | 24989 | 26608 | dhbE | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 96.73 | 100.00 | NP_391078 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| IABFJGIH_29 | 28139 | 27003 | tufA | 0.0 | elongation factor Tu | 84.96 | 95.69 | AHM69299 |
| IABFJGIH_13 | 11393 | 10524 | pdxS | 1.97e-151 | encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS | 83.45 | 98.31 | ACE61268 |
| IABFJGIH_3 | 151348 | 152124 | CodY | 1.99e-125 | nutrient-responsive regulator | 81.08 | 100.00 | ACJ80679 |
| IABFJGIH_42 | 45067 | 44498 | ClpP | 2.95e-100 | part of proteolytic complex | 81.05 | 97.44 | BAB94595 |
| IABFJGIH_39 | 41402 | 41010 | spxA1 | 4.11e-71 | redox-responsive transcription factor | 83.97 | 100.00 | AAF21893 |
| IABFJGIH_39 | 41402 | 41010 | spx | 2.45e-69 | essential gene which is a thiol/oxidative stress sensor | 80.15 | 100.00 | BAB57159 |
| IABFJGIH_34 | 34726 | 34484 | SpoVG | 7.52e-39 | RNA-binding protein | 81.48 | 79.41 | AAT02994 |
| IABFJGIH_28 | 40421 | 40224 | CspA | 2.34e-32 | cold shock protein | 90.91 | 100.00 | CAA62903 |
| IABFJGIH_22 | 44346 | 44540 | CspD | 1.09e-28 | cold shock protein | 81.54 | 98.48 | CAC99957 |
| IABFJGIH_40 | 13905 | 14099 | CspB | 4.22e-28 | cold shock protein | 83.08 | 98.48 | CAD00094 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| IABFJGIH_36 | 1282 | 1449 | Col(SD853) | 168 / 194 | 86.90 | NC015392 |
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| IABFJGIH_1 | 0.0 | 1.0 | 0.0 |
| IABFJGIH_2 | 1.0 | 0.0 | 1.0 |
| IABFJGIH_3 | 0.0 | 1.0 | 0.0 |
| IABFJGIH_4 | 0.0 | 1.0 | 0.0 |
| IABFJGIH_5 | 1.0 | 0.0 | 1.0 |
| IABFJGIH_6 | 0.0 | 1.0 | 0.0 |
| IABFJGIH_7 | 1.0 | 0.0 | 1.0 |
| IABFJGIH_8 | 1.0 | 0.0 | 1.0 |
| IABFJGIH_9 | 1.0 | 0.0 | 1.0 |
| IABFJGIH_11 | 0.0 | 1.0 | 0.0 |
| IABFJGIH_12 | 0.0 | 1.0 | 0.0 |
| IABFJGIH_13 | 0.0 | 1.0 | 0.0 |
| IABFJGIH_14 | 0.0 | 1.0 | 0.0 |
| IABFJGIH_15 | 0.0 | 1.0 | 0.0 |
| IABFJGIH_16 | 0.0 | 1.0 | 0.0 |
| IABFJGIH_17 | 0.0 | 1.0 | 0.0 |
| IABFJGIH_18 | 0.0 | 1.0 | 0.0 |
| IABFJGIH_19 | 0.0 | 1.0 | 0.0 |
| IABFJGIH_20 | 0.0 | 1.0 | 0.0 |
| IABFJGIH_21 | 0.0 | 1.0 | 0.0 |
| IABFJGIH_22 | 0.0 | 1.0 | 0.0 |
| IABFJGIH_23 | 0.0 | 1.0 | 0.0 |
| IABFJGIH_24 | 0.0 | 1.0 | 0.0 |
| IABFJGIH_25 | 0.0 | 1.0 | 0.0 |
| IABFJGIH_26 | 0.0 | 1.0 | 0.0 |
| IABFJGIH_27 | 0.0 | 1.0 | 0.0 |
| IABFJGIH_28 | 0.0 | 1.0 | 0.0 |
| IABFJGIH_29 | 0.0 | 1.0 | 0.0 |
| IABFJGIH_30 | 0.0 | 1.0 | 0.0 |
| IABFJGIH_31 | 0.0 | 1.0 | 0.0 |
| IABFJGIH_32 | 0.0 | 1.0 | 0.0 |
| IABFJGIH_33 | 0.0 | 1.0 | 0.0 |
| IABFJGIH_34 | 0.0 | 1.0 | 0.0 |
| IABFJGIH_35 | 0.0 | 1.0 | 0.0 |
| IABFJGIH_36 | 1.0 | 0.0 | 1.0 |
| IABFJGIH_37 | 0.0 | 1.0 | 0.0 |
| IABFJGIH_38 | 0.0 | 1.0 | 0.0 |
| IABFJGIH_39 | 0.0 | 1.0 | 0.0 |
| IABFJGIH_40 | 0.0 | 1.0 | 0.0 |
| IABFJGIH_41 | 0.0 | 1.0 | 0.0 |
| IABFJGIH_42 | 0.0 | 1.0 | 0.0 |
| IABFJGIH_43 | 1.0 | 0.0 | 1.0 |
| IABFJGIH_44 | 0.0 | 1.0 | 0.0 |
| IABFJGIH_45 | 0.0 | 1.0 | 0.0 |
| IABFJGIH_46 | 0.0 | 1.0 | 0.0 |
| IABFJGIH_47 | 0.0 | 1.0 | 0.0 |
| IABFJGIH_48 | 0.0 | 1.0 | 0.0 |
| IABFJGIH_49 | 1.0 | 0.0 | 1.0 |
| IABFJGIH_50 | 0.0 | 1.0 | 0.0 |
| IABFJGIH_51 | 0.0 | 1.0 | 0.0 |
| IABFJGIH_52 | 0.0 | 1.0 | 0.0 |
| IABFJGIH_53 | 0.0 | 1.0 | 0.0 |
| IABFJGIH_54 | 0.0 | 1.0 | 0.0 |
| IABFJGIH_57 | 0.0 | 1.0 | 0.0 |
| IABFJGIH_58 | 0.0 | 1.0 | 0.0 |
| IABFJGIH_59 | 0.0 | 1.0 | 0.0 |
| IABFJGIH_60 | 0.0 | 1.0 | 0.0 |
| IABFJGIH_61 | 1.0 | 0.0 | 1.0 |
| IABFJGIH_62 | 0.0 | 1.0 | 0.0 |
| IABFJGIH_63 | 0.0 | 1.0 | 0.0 |
| IABFJGIH_64 | 1.0 | 0.0 | 1.0 |
| IABFJGIH_65 | 0.0 | 1.0 | 0.0 |
| IABFJGIH_66 | 0.0 | 1.0 | 0.0 |
| IABFJGIH_67 | 0.0 | 1.0 | 0.0 |
| IABFJGIH_68 | 0.0 | 1.0 | 0.0 |
| IABFJGIH_69 | 1.0 | 0.0 | 1.0 |
| IABFJGIH_70 | 0.0 | 1.0 | 0.0 |
| IABFJGIH_71 | 0.0 | 1.0 | 0.0 |
| IABFJGIH_72 | 1.0 | 0.0 | 1.0 |
| IABFJGIH_73 | 1.0 | 0.0 | 1.0 |
| IABFJGIH_74 | 0.0 | 1.0 | 0.0 |
| IABFJGIH_75 | 0.0 | 1.0 | 0.0 |
| IABFJGIH_76 | 1.0 | 0.0 | 1.0 |
| IABFJGIH_77 | 0.0 | 1.0 | 0.0 |
| IABFJGIH_78 | 0.0 | 1.0 | 0.0 |
| IABFJGIH_79 | 0.0 | 1.0 | 0.0 |
| IABFJGIH_80 | 0.0 | 1.0 | 0.0 |
| IABFJGIH_83 | 0.0 | 1.0 | 0.0 |
| IABFJGIH_84 | 1.0 | 0.0 | 1.0 |
| IABFJGIH_85 | 0.0 | 1.0 | 0.0 |
| IABFJGIH_86 | 0.0 | 1.0 | 0.0 |
| IABFJGIH_87 | 0.0 | 1.0 | 0.0 |
| IABFJGIH_90 | 1.0 | 0.0 | 1.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| IABFJGIH_19 | 4531 | 15061 | True | Siphoviridae | VOG6587, VOG0189, VOG4566, VOG7581, VOG9667, VOG9667, VOG1563, VOG0275, VOG0275 |
| IABFJGIH_23 | 114559 | 141242 | False | Myoviridae | VOG0753, VOG4705, VOG7718, VOG4862, VOG4691, VOG4550, VOG3894, VOG4885, VOG4865, VOG6163, VOG3892, VOG3891, VOG3890, VOG0749, VOG4685, VOG4773, VOG10227, VOG0796, VOG5447, VOG4843, VOG0189, VOG3490 |
Phigaro v2.4.0 (Database: Jan. 2024 release)