Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 11 | 10 | 8 | 16.88 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| GAJBFBPL_26 | 158028 | 158621 | tmrB | ARO:3003059 | tmrB | 96.95 | 100.00 | AL009126.3 |
| GAJBFBPL_26 | 110147 | 111586 | lmrB | ARO:3002813 | lmrB | 98.74 | 100.42 | JYFL01000006.1 |
| GAJBFBPL_12 | 94293 | 94610 | ykkD | ARO:3003064 | ykkD | 98.10 | 100.00 | AL009126.1 |
| GAJBFBPL_12 | 93955 | 94293 | ykkC | ARO:3003063 | ykkC | 99.11 | 100.00 | AL009126.1 |
| GAJBFBPL_3 | 92110 | 94680 | Bacillus subtilis mprF | ARO:3003324 | Bsub_mprF | 99.77 | 100.00 | AL009126.3 |
| GAJBFBPL_45 | 21084 | 22286 | blt | ARO:3003006 | blt | 100.00 | 100.00 | L32599.1 |
| GAJBFBPL_42 | 9008 | 10177 | bmr | ARO:3003007 | bmr | 99.74 | 100.00 | M33768.1 |
| GAJBFBPL_45 | 2668 | 3522 | aadK | ARO:3002627 | aadK | 97.89 | 100.00 | AL009126.1 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| GAJBFBPL_45 | 2668 | 3520 | aadK | Streptomycin | 98.48 | 99.77 | M26879 |
| GAJBFBPL_26 | 97336 | 98255 | mph(K) | Spiramycin, Telithromycin | 97.61 | 99.89 | NC_000964 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| GAJBFBPL_45 | 2668 | 3519 | aadK | aminoglycoside 6-adenylyltransferase AadK | BLASTX | 97.89 | 100.00 | WP_003229862.1 |
| GAJBFBPL_54 | 29007 | 30647 | vmlR | ABC-F type ribosomal protection protein VmlR | INTERNAL_STOP | 97.81 | 100.00 | WP_003234144.1 |
| GAJBFBPL_15 | 37108 | 37626 | satA | streptothricin N-acetyltransferase SatA | BLASTX | 93.06 | 100.00 | WP_003242546.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| GAJBFBPL_7 | 60888 | 62030 | capA | 0.0 | Capsule | Immune modulation | 99.74 | 100.00 | NP_391469 |
| GAJBFBPL_7 | 59224 | 60405 | capB | 0.0 | Capsule | Immune modulation | 99.66 | 100.00 | NP_391471 |
| GAJBFBPL_27 | 60225 | 61163 | dhbB | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.57 | 100.00 | NP_391077 |
| GAJBFBPL_7 | 60420 | 60869 | capC | 0.0 | Capsule | Immune modulation | 99.56 | 100.00 | NP_391470 |
| GAJBFBPL_27 | 56553 | 57338 | dhbA | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.36 | 100.00 | NP_391080 |
| GAJBFBPL_55 | 21366 | 23129 | dep/capD | 0.0 | Capsule | Immune modulation | 99.26 | 100.00 | NP_389723 |
| GAJBFBPL_27 | 61183 | 68319 | dhbF | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.03 | 100.00 | NP_391076 |
| GAJBFBPL_27 | 57364 | 58560 | dhbC | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.58 | 100.00 | NP_391079 |
| GAJBFBPL_48 | 32477 | 33118 | hlyIII | 0.0 | Hemolysin III | Exotoxin | 97.82 | 100.00 | NP_390062 |
| GAJBFBPL_27 | 58578 | 60197 | dhbE | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 97.78 | 100.00 | NP_391078 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| GAJBFBPL_1 | 31903 | 33039 | tufA | 0.0 | elongation factor Tu | 85.75 | 95.69 | AHM69299 |
| GAJBFBPL_13 | 4249 | 5118 | pdxS | 1.92e-151 | encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS | 83.45 | 98.31 | ACE61268 |
| GAJBFBPL_21 | 150281 | 151057 | CodY | 2.15e-125 | nutrient-responsive regulator | 81.08 | 100.00 | ACJ80679 |
| GAJBFBPL_6 | 63562 | 62993 | ClpP | 1.58e-99 | part of proteolytic complex | 81.05 | 97.44 | BAB94595 |
| GAJBFBPL_11 | 9698 | 10090 | spxA1 | 3.54e-71 | redox-responsive transcription factor | 83.97 | 100.00 | AAF21893 |
| GAJBFBPL_11 | 9698 | 10090 | spx | 2.11e-69 | essential gene which is a thiol/oxidative stress sensor | 80.15 | 100.00 | BAB57159 |
| GAJBFBPL_14 | 34651 | 34409 | SpoVG | 2.20e-38 | RNA-binding protein | 80.25 | 79.41 | AAT02994 |
| GAJBFBPL_46 | 3977 | 4174 | CspA | 6.74e-32 | cold shock protein | 89.39 | 100.00 | CAA62903 |
| GAJBFBPL_48 | 44970 | 45164 | CspD | 3.36e-28 | cold shock protein | 80.00 | 98.48 | CAC99957 |
| GAJBFBPL_48 | 44970 | 45167 | CspB | 6.96e-28 | cold shock protein | 80.30 | 100.00 | CAD00094 |
| GAJBFBPL_46 | 3977 | 4171 | CspR | 2.12e-27 | cold shock protein | 80.00 | 98.48 | AAO82613 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| GAJBFBPL_1 | 0.0 | 1.0 | 0.0 |
| GAJBFBPL_2 | 0.0 | 1.0 | 0.0 |
| GAJBFBPL_3 | 0.0 | 1.0 | 0.0 |
| GAJBFBPL_4 | 0.0 | 1.0 | 0.0 |
| GAJBFBPL_5 | 0.0 | 1.0 | 0.0 |
| GAJBFBPL_6 | 0.0 | 1.0 | 0.0 |
| GAJBFBPL_7 | 0.0 | 1.0 | 0.0 |
| GAJBFBPL_8 | 0.0 | 1.0 | 0.0 |
| GAJBFBPL_9 | 0.0 | 1.0 | 0.0 |
| GAJBFBPL_10 | 0.0 | 1.0 | 0.0 |
| GAJBFBPL_11 | 0.0 | 1.0 | 0.0 |
| GAJBFBPL_12 | 0.0 | 1.0 | 0.0 |
| GAJBFBPL_13 | 0.0 | 1.0 | 0.0 |
| GAJBFBPL_14 | 0.0 | 1.0 | 0.0 |
| GAJBFBPL_15 | 0.0 | 1.0 | 0.0 |
| GAJBFBPL_16 | 0.0 | 1.0 | 0.0 |
| GAJBFBPL_17 | 0.0 | 1.0 | 0.0 |
| GAJBFBPL_18 | 0.0 | 1.0 | 0.0 |
| GAJBFBPL_19 | 0.0 | 1.0 | 0.0 |
| GAJBFBPL_20 | 0.0 | 1.0 | 0.0 |
| GAJBFBPL_21 | 0.0 | 1.0 | 0.0 |
| GAJBFBPL_22 | 0.0 | 1.0 | 0.0 |
| GAJBFBPL_23 | 0.0 | 1.0 | 0.0 |
| GAJBFBPL_24 | 0.0 | 1.0 | 0.0 |
| GAJBFBPL_25 | 0.0 | 1.0 | 0.0 |
| GAJBFBPL_26 | 0.0 | 1.0 | 0.0 |
| GAJBFBPL_27 | 0.0 | 1.0 | 0.0 |
| GAJBFBPL_28 | 0.0 | 1.0 | 0.0 |
| GAJBFBPL_29 | 0.0 | 1.0 | 0.0 |
| GAJBFBPL_31 | 0.0 | 1.0 | 0.0 |
| GAJBFBPL_32 | 0.0 | 1.0 | 0.0 |
| GAJBFBPL_33 | 1.0 | 0.0 | 1.0 |
| GAJBFBPL_34 | 0.0 | 1.0 | 0.0 |
| GAJBFBPL_35 | 0.0 | 1.0 | 0.0 |
| GAJBFBPL_36 | 0.0 | 1.0 | 0.0 |
| GAJBFBPL_37 | 0.0 | 1.0 | 0.0 |
| GAJBFBPL_38 | 0.0 | 1.0 | 0.0 |
| GAJBFBPL_39 | 0.0 | 1.0 | 0.0 |
| GAJBFBPL_40 | 0.0 | 1.0 | 0.0 |
| GAJBFBPL_41 | 0.0 | 1.0 | 0.0 |
| GAJBFBPL_42 | 0.0 | 1.0 | 0.0 |
| GAJBFBPL_43 | 0.0 | 1.0 | 0.0 |
| GAJBFBPL_44 | 0.0 | 1.0 | 0.0 |
| GAJBFBPL_45 | 0.0 | 1.0 | 0.0 |
| GAJBFBPL_46 | 0.0 | 1.0 | 0.0 |
| GAJBFBPL_47 | 0.0 | 1.0 | 0.0 |
| GAJBFBPL_48 | 0.0 | 1.0 | 0.0 |
| GAJBFBPL_49 | 0.0 | 1.0 | 0.0 |
| GAJBFBPL_50 | 0.0 | 1.0 | 0.0 |
| GAJBFBPL_51 | 0.0 | 1.0 | 0.0 |
| GAJBFBPL_52 | 0.0 | 1.0 | 0.0 |
| GAJBFBPL_53 | 1.0 | 0.0 | 1.0 |
| GAJBFBPL_54 | 0.0 | 1.0 | 0.0 |
| GAJBFBPL_55 | 0.0 | 1.0 | 0.0 |
| GAJBFBPL_56 | 1.0 | 0.0 | 1.0 |
| GAJBFBPL_58 | 1.0 | 0.0 | 1.0 |
| GAJBFBPL_59 | 0.0 | 1.0 | 0.0 |
| GAJBFBPL_60 | 0.0 | 1.0 | 0.0 |
| GAJBFBPL_61 | 0.0 | 1.0 | 0.0 |
| GAJBFBPL_62 | 0.0 | 1.0 | 0.0 |
| GAJBFBPL_63 | 0.0 | 1.0 | 0.0 |
| GAJBFBPL_64 | 0.0 | 1.0 | 0.0 |
| GAJBFBPL_65 | 1.0 | 0.0 | 1.0 |
| GAJBFBPL_66 | 0.0 | 1.0 | 0.0 |
| GAJBFBPL_67 | 0.0 | 1.0 | 0.0 |
| GAJBFBPL_68 | 0.0 | 1.0 | 0.0 |
| GAJBFBPL_69 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| GAJBFBPL_9 | 142205 | 163224 | False | Siphoviridae | VOG0198, VOG0713, VOG4841, VOG4581, VOG1886, VOG0202, VOG4556, VOG4573, VOG4553, VOG5361, VOG0204, VOG4589, VOG0539, VOG0704, VOG4586, VOG0541, VOG5910, VOG6163, VOG0801, VOG4619 |
| GAJBFBPL_12 | 38053 | 64469 | False | Myoviridae | VOG4602, VOG9667, VOG3490, VOG0189, VOG4861, VOG5447, VOG0796, VOG10227, VOG4773, VOG4685, VOG0749, VOG3890, VOG3891, VOG3892, VOG6163, VOG4865, VOG4885, VOG3894, VOG4550, VOG4691, VOG4862, VOG7718, VOG4705 |
| GAJBFBPL_16 | 8064 | 33441 | False | Myoviridae | VOG4705, VOG3390, VOG7718, VOG4862, VOG4691, VOG3894, VOG4885, VOG4865, VOG6163, VOG3892, VOG5668, VOG3891, VOG3890, VOG10055, VOG4870, VOG4589, VOG0749, VOG4685, VOG4688, VOG4773, VOG9315 |
| GAJBFBPL_16 | 46226 | 52250 | False | Unknown | VOG1292, VOG1309, VOG0083, VOG1878, VOG3549, VOG8241 |
Phigaro v2.4.0 (Database: Jan. 2024 release)