Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 12 | 10 | 8 | 17.55 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| BANGCKBB_28 | 100631 | 101800 | bmr | ARO:3003007 | bmr | 99.74 | 100.00 | M33768.1 |
| BANGCKBB_29 | 100295 | 100888 | tmrB | ARO:3003059 | tmrB | 96.95 | 100.00 | AL009126.3 |
| BANGCKBB_36 | 55263 | 55601 | ykkC | ARO:3003063 | ykkC | 97.32 | 100.00 | AL009126.1 |
| BANGCKBB_36 | 54946 | 55263 | ykkD | ARO:3003064 | ykkD | 98.10 | 100.00 | AL009126.1 |
| BANGCKBB_29 | 52468 | 53907 | lmrB | ARO:3002813 | lmrB | 98.74 | 100.42 | JYFL01000006.1 |
| BANGCKBB_50 | 52015 | 53661 | vmlR | ARO:3004476 | vmlR | 98.18 | 100.18 | NC_000964.3 |
| BANGCKBB_38 | 44360 | 46930 | Bacillus subtilis mprF | ARO:3003324 | Bsub_mprF | 99.77 | 100.00 | AL009126.3 |
| BANGCKBB_29 | 39653 | 40576 | mphK | ARO:3004541 | mphK | 96.42 | 100.33 | CP010314.1 |
| BANGCKBB_71 | 12097 | 13299 | blt | ARO:3003006 | blt | 100.00 | 100.00 | L32599.1 |
| BANGCKBB_7 | 368 | 1222 | aadK | ARO:3002627 | aadK | 98.59 | 100.00 | AL009126.1 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| BANGCKBB_32 | 34544 | 35920 | tet(L) | Doxycycline, Tetracycline, Minocycline, Tigecycline, Doxycycline, Tetracycline | 99.35 | 100.00 | X08034 |
| BANGCKBB_7 | 368 | 1222 | aadK | Streptomycin | 98.71 | 100.00 | M26879 |
| BANGCKBB_29 | 39653 | 40576 | mph(K) | Spiramycin, Telithromycin | 96.97 | 100.00 | NC_000964 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| BANGCKBB_32 | 34544 | 35917 | tet(L) | tetracycline efflux MFS transporter Tet(L) | BLASTX | 99.13 | 100.00 | WP_003242953.1 |
| BANGCKBB_7 | 371 | 1222 | aadK | aminoglycoside 6-adenylyltransferase AadK | BLASTX | 98.59 | 100.00 | WP_003229862.1 |
| BANGCKBB_50 | 52018 | 53661 | vmlR | ABC-F type ribosomal protection protein VmlR | BLASTX | 98.18 | 100.00 | WP_003234144.1 |
| BANGCKBB_29 | 39653 | 40573 | mphK | macrolide 2'-phosphotransferase MphK | BLASTX | 96.42 | 100.00 | WP_003246254.1 |
| BANGCKBB_32 | 37901 | 38419 | satA | streptothricin N-acetyltransferase SatA | BLASTX | 90.17 | 100.00 | WP_003242546.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| BANGCKBB_9 | 3228 | 4409 | capB | 0.0 | Capsule | Immune modulation | 99.66 | 100.00 | NP_391471 |
| BANGCKBB_9 | 1603 | 2745 | capA | 0.0 | Capsule | Immune modulation | 99.65 | 100.00 | NP_391469 |
| BANGCKBB_9 | 2764 | 3213 | capC | 0.0 | Capsule | Immune modulation | 99.56 | 100.00 | NP_391470 |
| BANGCKBB_39 | 10829 | 11470 | hlyIII | 0.0 | Hemolysin III | Exotoxin | 99.53 | 100.00 | NP_390062 |
| BANGCKBB_14 | 60100 | 60885 | dhbA | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.24 | 100.00 | NP_391080 |
| BANGCKBB_58 | 36007 | 37770 | dep/capD | 0.0 | Capsule | Immune modulation | 99.21 | 100.00 | NP_389723 |
| BANGCKBB_14 | 64730 | 71866 | dhbF | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.61 | 100.00 | NP_391076 |
| BANGCKBB_14 | 60911 | 62107 | dhbC | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.58 | 100.00 | NP_391079 |
| BANGCKBB_14 | 63772 | 64710 | dhbB | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.30 | 100.00 | NP_391077 |
| BANGCKBB_14 | 62125 | 63744 | dhbE | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 97.90 | 100.00 | NP_391078 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| BANGCKBB_1 | 28228 | 27092 | tufA | 0.0 | elongation factor Tu | 85.75 | 95.69 | AHM69299 |
| BANGCKBB_75 | 4249 | 5118 | pdxS | 1.94e-151 | encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS | 83.45 | 98.31 | ACE61268 |
| BANGCKBB_8 | 103626 | 102850 | CodY | 2.06e-125 | nutrient-responsive regulator | 81.08 | 100.00 | ACJ80679 |
| BANGCKBB_2 | 63940 | 63371 | ClpP | 6.85e-100 | part of proteolytic complex | 81.05 | 97.44 | BAB94595 |
| BANGCKBB_34 | 51768 | 52160 | spxA1 | 5.75e-71 | redox-responsive transcription factor | 83.97 | 100.00 | AAF21893 |
| BANGCKBB_34 | 51768 | 52160 | spx | 3.43e-69 | essential gene which is a thiol/oxidative stress sensor | 80.15 | 100.00 | BAB57159 |
| BANGCKBB_5 | 34663 | 34421 | SpoVG | 7.49e-39 | RNA-binding protein | 81.48 | 79.41 | AAT02994 |
| BANGCKBB_50 | 3963 | 4160 | CspA | 3.53e-32 | cold shock protein | 90.91 | 100.00 | CAA62903 |
| BANGCKBB_39 | 22703 | 22897 | CspD | 5.92e-29 | cold shock protein | 80.00 | 98.48 | CAC99957 |
| BANGCKBB_39 | 22703 | 22900 | CspB | 1.23e-28 | cold shock protein | 80.30 | 100.00 | CAD00094 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| BANGCKBB_1 | 0.0 | 1.0 | 0.0 |
| BANGCKBB_2 | 0.0 | 1.0 | 0.0 |
| BANGCKBB_3 | 0.0 | 1.0 | 0.0 |
| BANGCKBB_4 | 1.0 | 0.0 | 1.0 |
| BANGCKBB_5 | 0.0 | 1.0 | 0.0 |
| BANGCKBB_6 | 0.0 | 1.0 | 0.0 |
| BANGCKBB_7 | 0.0 | 1.0 | 0.0 |
| BANGCKBB_8 | 0.0 | 1.0 | 0.0 |
| BANGCKBB_9 | 0.0 | 1.0 | 0.0 |
| BANGCKBB_10 | 0.0 | 1.0 | 0.0 |
| BANGCKBB_11 | 1.0 | 0.0 | 1.0 |
| BANGCKBB_12 | 1.0 | 0.0 | 1.0 |
| BANGCKBB_13 | 0.0 | 1.0 | 0.0 |
| BANGCKBB_14 | 0.0 | 1.0 | 0.0 |
| BANGCKBB_15 | 0.0 | 1.0 | 0.0 |
| BANGCKBB_16 | 1.0 | 0.0 | 1.0 |
| BANGCKBB_17 | 0.0 | 1.0 | 0.0 |
| BANGCKBB_18 | 0.0 | 1.0 | 0.0 |
| BANGCKBB_20 | 0.0 | 1.0 | 0.0 |
| BANGCKBB_23 | 0.0 | 1.0 | 0.0 |
| BANGCKBB_24 | 0.0 | 1.0 | 0.0 |
| BANGCKBB_25 | 0.0 | 1.0 | 0.0 |
| BANGCKBB_26 | 0.0 | 1.0 | 0.0 |
| BANGCKBB_27 | 0.0 | 1.0 | 0.0 |
| BANGCKBB_28 | 0.0 | 1.0 | 0.0 |
| BANGCKBB_29 | 0.0 | 1.0 | 0.0 |
| BANGCKBB_31 | 0.0 | 1.0 | 0.0 |
| BANGCKBB_32 | 0.0 | 1.0 | 0.0 |
| BANGCKBB_33 | 1.0 | 0.0 | 1.0 |
| BANGCKBB_34 | 0.0 | 1.0 | 0.0 |
| BANGCKBB_35 | 0.0 | 1.0 | 0.0 |
| BANGCKBB_36 | 0.0 | 1.0 | 0.0 |
| BANGCKBB_37 | 0.0 | 1.0 | 0.0 |
| BANGCKBB_38 | 0.0 | 1.0 | 0.0 |
| BANGCKBB_39 | 0.0 | 1.0 | 0.0 |
| BANGCKBB_40 | 0.0 | 1.0 | 0.0 |
| BANGCKBB_41 | 0.0 | 1.0 | 0.0 |
| BANGCKBB_42 | 0.0 | 1.0 | 0.0 |
| BANGCKBB_43 | 0.0 | 1.0 | 0.0 |
| BANGCKBB_44 | 1.0 | 0.0 | 1.0 |
| BANGCKBB_45 | 0.0 | 1.0 | 0.0 |
| BANGCKBB_46 | 0.0 | 1.0 | 0.0 |
| BANGCKBB_47 | 0.0 | 1.0 | 0.0 |
| BANGCKBB_48 | 0.0 | 1.0 | 0.0 |
| BANGCKBB_49 | 0.0 | 1.0 | 0.0 |
| BANGCKBB_50 | 0.0 | 1.0 | 0.0 |
| BANGCKBB_51 | 0.0 | 1.0 | 0.0 |
| BANGCKBB_52 | 0.0 | 1.0 | 0.0 |
| BANGCKBB_53 | 0.0 | 1.0 | 0.0 |
| BANGCKBB_54 | 0.0 | 1.0 | 0.0 |
| BANGCKBB_55 | 0.0 | 1.0 | 0.0 |
| BANGCKBB_56 | 0.0 | 1.0 | 0.0 |
| BANGCKBB_57 | 0.0 | 1.0 | 0.0 |
| BANGCKBB_58 | 0.0 | 1.0 | 0.0 |
| BANGCKBB_59 | 0.0 | 1.0 | 0.0 |
| BANGCKBB_60 | 0.0 | 1.0 | 0.0 |
| BANGCKBB_61 | 0.0 | 1.0 | 0.0 |
| BANGCKBB_62 | 0.0 | 1.0 | 0.0 |
| BANGCKBB_63 | 0.0 | 1.0 | 0.0 |
| BANGCKBB_65 | 0.0 | 1.0 | 0.0 |
| BANGCKBB_66 | 1.0 | 0.0 | 1.0 |
| BANGCKBB_67 | 0.0 | 1.0 | 0.0 |
| BANGCKBB_68 | 0.0 | 1.0 | 0.0 |
| BANGCKBB_69 | 0.0 | 1.0 | 0.0 |
| BANGCKBB_70 | 0.0 | 1.0 | 0.0 |
| BANGCKBB_71 | 0.0 | 1.0 | 0.0 |
| BANGCKBB_72 | 0.0 | 1.0 | 0.0 |
| BANGCKBB_74 | 0.0 | 1.0 | 0.0 |
| BANGCKBB_75 | 0.0 | 1.0 | 0.0 |
| BANGCKBB_76 | 0.0 | 1.0 | 0.0 |
| BANGCKBB_77 | 0.0 | 1.0 | 0.0 |
| BANGCKBB_79 | 0.0 | 1.0 | 0.0 |
| BANGCKBB_80 | 0.0 | 1.0 | 0.0 |
| BANGCKBB_81 | 0.0 | 1.0 | 0.0 |
| BANGCKBB_82 | 0.0 | 1.0 | 0.0 |
| BANGCKBB_83 | 1.0 | 0.0 | 1.0 |
| BANGCKBB_84 | 0.0 | 1.0 | 0.0 |
| BANGCKBB_85 | 0.0 | 1.0 | 0.0 |
| BANGCKBB_86 | 0.0 | 1.0 | 0.0 |
| BANGCKBB_87 | 0.0 | 1.0 | 0.0 |
| BANGCKBB_88 | 0.0 | 1.0 | 0.0 |
| BANGCKBB_89 | 1.0 | 0.0 | 1.0 |
| BANGCKBB_90 | 0.0 | 1.0 | 0.0 |
| BANGCKBB_91 | 0.0 | 1.0 | 0.0 |
| BANGCKBB_92 | 0.0 | 1.0 | 0.0 |
| BANGCKBB_93 | 1.0 | 0.0 | 1.0 |
| BANGCKBB_94 | 0.0 | 1.0 | 0.0 |
| BANGCKBB_95 | 1.0 | 0.0 | 1.0 |
| BANGCKBB_96 | 0.0 | 1.0 | 0.0 |
| BANGCKBB_98 | 0.0 | 1.0 | 0.0 |
| BANGCKBB_100 | 0.0 | 1.0 | 0.0 |
| BANGCKBB_101 | 1.0 | 0.0 | 1.0 |
| BANGCKBB_102 | 0.0 | 1.0 | 0.0 |
| BANGCKBB_103 | 1.0 | 0.0 | 1.0 |
| BANGCKBB_104 | 0.0 | 1.0 | 0.0 |
| BANGCKBB_105 | 0.0 | 1.0 | 0.0 |
| BANGCKBB_106 | 0.0 | 1.0 | 0.0 |
| BANGCKBB_107 | 1.0 | 0.0 | 1.0 |
| BANGCKBB_108 | 1.0 | 0.0 | 1.0 |
| BANGCKBB_109 | 1.0 | 0.0 | 1.0 |
| BANGCKBB_110 | 1.0 | 0.0 | 1.0 |
| BANGCKBB_111 | 0.0 | 1.0 | 0.0 |
| BANGCKBB_112 | 1.0 | 0.0 | 1.0 |
| BANGCKBB_113 | 1.0 | 0.0 | 1.0 |
| BANGCKBB_114 | 0.0 | 1.0 | 0.0 |
| BANGCKBB_115 | 1.0 | 0.0 | 1.0 |
| BANGCKBB_117 | 0.0 | 1.0 | 0.0 |
| BANGCKBB_118 | 1.0 | 0.0 | 1.0 |
| BANGCKBB_119 | 1.0 | 0.0 | 1.0 |
| BANGCKBB_120 | 0.0 | 1.0 | 0.0 |
| BANGCKBB_121 | 1.0 | 0.0 | 1.0 |
| BANGCKBB_125 | 0.0 | 1.0 | 0.0 |
| BANGCKBB_126 | 1.0 | 0.0 | 1.0 |
| BANGCKBB_129 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| BANGCKBB_7 | 117108 | 144491 | False | Siphoviridae | VOG4574, VOG0136, VOG5221, VOG4705, VOG3390, VOG4612, VOG4660, VOG4619, VOG0801, VOG6163, VOG5910, VOG0541, VOG4586, VOG0704, VOG0539, VOG4589, VOG0204, VOG5361, VOG4553, VOG4568, VOG4556, VOG1886, VOG4581, VOG4841, VOG2703 |
| BANGCKBB_7 | 196674 | 225265 | False | Siphoviridae | VOG0705, VOG6116, VOG0602, VOG4574, VOG0753, VOG4705, VOG4612, VOG4660, VOG4619, VOG0801, VOG11074, VOG5361, VOG3676, VOG4555, VOG0692, VOG2613, VOG3699, VOG5023, VOG0275, VOG1183 |
| BANGCKBB_36 | 85080 | 110750 | False | Myoviridae | VOG0753, VOG4705, VOG7718, VOG4862, VOG4691, VOG4691, VOG4550, VOG3894, VOG4885, VOG4865, VOG6163, VOG3892, VOG3891, VOG3890, VOG0749, VOG4685, VOG4773, VOG10227, VOG0796, VOG5447, VOG4861, VOG0189 |
| BANGCKBB_48 | 1222 | 24961 | False | Myoviridae | VOG0136, VOG0753, VOG4705, VOG3390, VOG7718, VOG4845, VOG4691, VOG4550, VOG0573, VOG1348, VOG1433, VOG1956, VOG6163, VOG4699, VOG1352, VOG1883, VOG1881, VOG1353, VOG4623, VOG4564, VOG4553, VOG4555, VOG0720, VOG10227, VOG0796, VOG10945, VOG6282 |
| BANGCKBB_54 | 6243 | 14271 | False | Myoviridae | VOG1563, VOG8281, VOG0705, VOG9692, VOG5219, VOG5220, VOG5243 |
Phigaro v2.4.0 (Database: Jan. 2024 release)