Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 13 | 10 | 9 | 18.71 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| EHPLCIIP_5 | 422240 | 423094 | aadK | ARO:3002627 | aadK | 100.00 | 100.00 | AL009126.1 |
| EHPLCIIP_5 | 403531 | 404733 | blt | ARO:3003006 | blt | 100.00 | 100.00 | L32599.1 |
| EHPLCIIP_3 | 240667 | 241005 | ykkC | ARO:3003063 | ykkC | 100.00 | 100.00 | AL009126.1 |
| EHPLCIIP_3 | 240350 | 240667 | ykkD | ARO:3003064 | ykkD | 99.05 | 100.00 | AL009126.1 |
| EHPLCIIP_5 | 229244 | 230413 | bmr | ARO:3003007 | bmr | 99.74 | 100.00 | M33768.1 |
| EHPLCIIP_10 | 108922 | 109842 | mphK | ARO:3004541 | mphK | 100.00 | 100.00 | CP010314.1 |
| EHPLCIIP_10 | 95588 | 97027 | lmrB | ARO:3002813 | lmrB | 99.79 | 100.42 | JYFL01000006.1 |
| EHPLCIIP_10 | 45930 | 46523 | tmrB | ARO:3003059 | tmrB | 100.00 | 100.00 | AL009126.3 |
| EHPLCIIP_23 | 29191 | 30834 | vmlR | ARO:3004476 | vmlR | 100.00 | 100.00 | NC_000964.3 |
| EHPLCIIP_26 | 15039 | 17609 | Bacillus subtilis mprF | ARO:3003324 | Bsub_mprF | 100.00 | 100.00 | AL009126.3 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| EHPLCIIP_5 | 422240 | 423094 | aadK | Streptomycin | 100.00 | 100.00 | M26879 |
| EHPLCIIP_10 | 108922 | 109842 | mph(K) | Spiramycin, Telithromycin | 100.00 | 100.00 | NC_000964 |
| EHPLCIIP_17 | 36325 | 37701 | tet(L) | Doxycycline, Tetracycline, Minocycline, Tigecycline, Doxycycline, Tetracycline | 100.00 | 100.00 | X08034 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| EHPLCIIP_5 | 422243 | 423094 | aadK | aminoglycoside 6-adenylyltransferase AadK | EXACTX | 100.00 | 100.00 | WP_003229862.1 |
| EHPLCIIP_10 | 108925 | 109842 | mphK | macrolide 2'-phosphotransferase MphK | EXACTX | 100.00 | 100.00 | WP_003246254.1 |
| EHPLCIIP_17 | 39701 | 40219 | satA | streptothricin N-acetyltransferase SatA | EXACTX | 100.00 | 100.00 | WP_003242546.1 |
| EHPLCIIP_17 | 36325 | 37698 | tet(L) | tetracycline efflux MFS transporter Tet(L) | EXACTX | 100.00 | 100.00 | WP_003242953.1 |
| EHPLCIIP_23 | 29191 | 30831 | vmlR | ABC-F type ribosomal protection protein VmlR | EXACTX | 100.00 | 100.00 | WP_003234144.1 |
| EHPLCIIP_27 | 8010 | 10604 | rphC | rifamycin-inactivating phosphotransferase RphC | BLASTX | 81.15 | 99.88 | WP_087347987.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| EHPLCIIP_7 | 519300 | 520442 | capA | 0.0 | Capsule | Immune modulation | 100.00 | 100.00 | NP_391469 |
| EHPLCIIP_7 | 520925 | 522106 | capB | 0.0 | Capsule | Immune modulation | 100.00 | 100.00 | NP_391471 |
| EHPLCIIP_7 | 520461 | 520910 | capC | 0.0 | Capsule | Immune modulation | 100.00 | 100.00 | NP_391470 |
| EHPLCIIP_27 | 55497 | 57260 | dep/capD | 0.0 | Capsule | Immune modulation | 100.00 | 100.00 | NP_389723 |
| EHPLCIIP_7 | 112965 | 113750 | dhbA | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 100.00 | 100.00 | NP_391080 |
| EHPLCIIP_7 | 109129 | 110067 | dhbB | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 100.00 | 100.00 | NP_391077 |
| EHPLCIIP_7 | 111743 | 112939 | dhbC | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 100.00 | 100.00 | NP_391079 |
| EHPLCIIP_7 | 110095 | 111714 | dhbE | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 100.00 | 100.00 | NP_391078 |
| EHPLCIIP_7 | 101973 | 109109 | dhbF | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 100.00 | 100.00 | NP_391076 |
| EHPLCIIP_5 | 29899 | 30540 | hlyIII | 0.0 | Hemolysin III | Exotoxin | 100.00 | 100.00 | NP_390062 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| EHPLCIIP_2 | 27995 | 26859 | tufA | 0.0 | elongation factor Tu | 84.96 | 95.69 | AHM69299 |
| EHPLCIIP_41 | 11322 | 10453 | pdxS | 1.11e-151 | encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS | 83.79 | 98.31 | ACE61268 |
| EHPLCIIP_1 | 72618 | 73394 | CodY | 2.74e-125 | nutrient-responsive regulator | 81.08 | 100.00 | ACJ80679 |
| EHPLCIIP_7 | 367688 | 368257 | ClpP | 4.79e-99 | part of proteolytic complex | 81.05 | 97.44 | BAB94595 |
| EHPLCIIP_3 | 389827 | 389435 | spxA1 | 1.50e-69 | redox-responsive transcription factor | 83.21 | 100.00 | AAF21893 |
| EHPLCIIP_14 | 20737 | 20979 | SpoVG | 7.55e-39 | RNA-binding protein | 81.48 | 79.41 | AAT02994 |
| EHPLCIIP_23 | 76306 | 76109 | CspA | 3.38e-32 | cold shock protein | 90.91 | 100.00 | CAA62903 |
| EHPLCIIP_39 | 12966 | 12772 | CspB | 1.87e-28 | cold shock protein | 83.08 | 98.48 | CAD00094 |
| EHPLCIIP_5 | 42502 | 42696 | CspD | 5.29e-28 | cold shock protein | 81.54 | 98.48 | CAC99957 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| EHPLCIIP_1 | 0.0 | 1.0 | 0.0 |
| EHPLCIIP_2 | 0.0 | 1.0 | 0.0 |
| EHPLCIIP_3 | 0.0 | 1.0 | 0.0 |
| EHPLCIIP_4 | 1.0 | 0.0 | 1.0 |
| EHPLCIIP_5 | 0.0 | 1.0 | 0.0 |
| EHPLCIIP_6 | 0.0 | 1.0 | 0.0 |
| EHPLCIIP_7 | 0.0 | 1.0 | 0.0 |
| EHPLCIIP_8 | 0.0 | 1.0 | 0.0 |
| EHPLCIIP_9 | 0.0 | 1.0 | 0.0 |
| EHPLCIIP_10 | 0.0 | 1.0 | 0.0 |
| EHPLCIIP_11 | 0.0 | 1.0 | 0.0 |
| EHPLCIIP_12 | 0.0 | 1.0 | 0.0 |
| EHPLCIIP_13 | 0.0 | 1.0 | 0.0 |
| EHPLCIIP_14 | 0.0 | 1.0 | 0.0 |
| EHPLCIIP_15 | 0.0 | 1.0 | 0.0 |
| EHPLCIIP_16 | 0.0 | 1.0 | 0.0 |
| EHPLCIIP_17 | 0.0 | 1.0 | 0.0 |
| EHPLCIIP_18 | 1.0 | 0.0 | 1.0 |
| EHPLCIIP_19 | 1.0 | 0.0 | 1.0 |
| EHPLCIIP_20 | 1.0 | 0.0 | 1.0 |
| EHPLCIIP_21 | 1.0 | 0.0 | 1.0 |
| EHPLCIIP_22 | 1.0 | 0.0 | 1.0 |
| EHPLCIIP_23 | 0.0 | 1.0 | 0.0 |
| EHPLCIIP_24 | 1.0 | 0.0 | 1.0 |
| EHPLCIIP_25 | 0.0 | 1.0 | 0.0 |
| EHPLCIIP_26 | 0.0 | 1.0 | 0.0 |
| EHPLCIIP_27 | 0.0 | 1.0 | 0.0 |
| EHPLCIIP_28 | 0.0 | 1.0 | 0.0 |
| EHPLCIIP_29 | 1.0 | 0.0 | 1.0 |
| EHPLCIIP_31 | 1.0 | 0.0 | 1.0 |
| EHPLCIIP_32 | 1.0 | 0.0 | 1.0 |
| EHPLCIIP_33 | 0.0 | 1.0 | 0.0 |
| EHPLCIIP_34 | 0.0 | 1.0 | 0.0 |
| EHPLCIIP_35 | 0.0 | 1.0 | 0.0 |
| EHPLCIIP_36 | 1.0 | 0.0 | 1.0 |
| EHPLCIIP_37 | 1.0 | 0.0 | 1.0 |
| EHPLCIIP_39 | 0.0 | 1.0 | 0.0 |
| EHPLCIIP_40 | 0.0 | 1.0 | 0.0 |
| EHPLCIIP_41 | 0.0 | 1.0 | 0.0 |
| EHPLCIIP_42 | 0.0 | 1.0 | 0.0 |
| EHPLCIIP_45 | 1.0 | 0.0 | 1.0 |
| EHPLCIIP_46 | 0.0 | 1.0 | 0.0 |
| EHPLCIIP_47 | 0.0 | 1.0 | 0.0 |
| EHPLCIIP_48 | 0.0 | 1.0 | 0.0 |
| EHPLCIIP_50 | 0.0 | 1.0 | 0.0 |
| EHPLCIIP_51 | 1.0 | 0.0 | 1.0 |
| EHPLCIIP_52 | 1.0 | 0.0 | 1.0 |
| EHPLCIIP_53 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| EHPLCIIP_1 | 264050 | 266158 | False | Siphoviridae | VOG9667, VOG5353, VOG0181 |
| EHPLCIIP_1 | 279541 | 302434 | False | Siphoviridae | VOG4841, VOG4581, VOG1886, VOG4556, VOG4568, VOG4553, VOG5361, VOG0204, VOG4589, VOG0539, VOG0704, VOG4586, VOG0541, VOG5910, VOG6163, VOG0801, VOG4619, VOG4660, VOG4612, VOG3390, VOG4705, VOG0641, VOG4918 |
| EHPLCIIP_3 | 270843 | 296951 | False | Myoviridae | VOG4705, VOG7718, VOG4862, VOG4691, VOG4550, VOG3894, VOG4885, VOG4865, VOG6163, VOG3892, VOG3891, VOG3890, VOG0749, VOG4685, VOG4773, VOG10227, VOG0796, VOG5447, VOG4861, VOG0189, VOG3490, VOG9667 |
| EHPLCIIP_16 | 55519 | 63981 | False | Unknown | VOG0048, VOG4908, VOG6211, VOG0322, VOG7476, VOG0025 |
Phigaro v2.4.0 (Database: Jan. 2024 release)