Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 13 | 10 | 7 | 17.83 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| BCHJFFHO_4 | 680532 | 681701 | bmr | ARO:3003007 | bmr | 99.74 | 100.00 | M33768.1 |
| BCHJFFHO_4 | 455964 | 457166 | blt | ARO:3003006 | blt | 100.00 | 100.00 | L32599.1 |
| BCHJFFHO_4 | 437567 | 438421 | aadK | ARO:3002627 | aadK | 97.89 | 100.00 | AL009126.1 |
| BCHJFFHO_2 | 428923 | 429240 | ykkD | ARO:3003064 | ykkD | 99.05 | 100.00 | AL009126.1 |
| BCHJFFHO_2 | 428585 | 428923 | ykkC | ARO:3003063 | ykkC | 100.00 | 100.00 | AL009126.1 |
| BCHJFFHO_7 | 340379 | 341299 | mphK | ARO:3004541 | mphK | 98.69 | 100.00 | CP010314.1 |
| BCHJFFHO_7 | 327046 | 328485 | lmrB | ARO:3002813 | lmrB | 99.16 | 100.42 | JYFL01000006.1 |
| BCHJFFHO_7 | 277367 | 277960 | tmrB | ARO:3003059 | tmrB | 97.97 | 100.00 | AL009126.3 |
| BCHJFFHO_7 | 29396 | 31042 | vmlR | ARO:3004476 | vmlR | 98.54 | 100.18 | NC_000964.3 |
| BCHJFFHO_8 | 27710 | 30280 | Bacillus subtilis mprF | ARO:3003324 | Bsub_mprF | 100.00 | 100.00 | AL009126.3 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| BCHJFFHO_6 | 36325 | 37701 | tet(L) | Doxycycline, Tetracycline, Minocycline, Tigecycline, Doxycycline, Tetracycline | 99.93 | 100.00 | X08034 |
| BCHJFFHO_7 | 340379 | 341299 | mph(K) | Spiramycin, Telithromycin | 99.02 | 100.00 | NC_000964 |
| BCHJFFHO_4 | 437567 | 438419 | aadK | Streptomycin | 98.59 | 99.77 | M26879 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| BCHJFFHO_6 | 36325 | 37698 | tet(L) | tetracycline efflux MFS transporter Tet(L) | EXACTX | 100.00 | 100.00 | WP_003242953.1 |
| BCHJFFHO_7 | 340382 | 341299 | mphK | macrolide 2'-phosphotransferase MphK | BLASTX | 98.69 | 100.00 | WP_003246254.1 |
| BCHJFFHO_7 | 29396 | 31039 | vmlR | ABC-F type ribosomal protection protein VmlR | BLASTX | 98.54 | 100.00 | WP_003234144.1 |
| BCHJFFHO_4 | 437567 | 438418 | aadK | aminoglycoside 6-adenylyltransferase AadK | BLASTX | 97.89 | 100.00 | WP_003229862.1 |
| BCHJFFHO_6 | 39654 | 40172 | satA | streptothricin N-acetyltransferase SatA | BLASTX | 90.17 | 100.00 | WP_003242546.1 |
| BCHJFFHO_4 | 986304 | 988901 | rphC | rifamycin-inactivating phosphotransferase RphC | INTERNAL_STOP | 81.40 | 100.00 | WP_087347987.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| BCHJFFHO_6 | 508640 | 509821 | capB | 0.0 | Capsule | Immune modulation | 99.83 | 100.00 | NP_391471 |
| BCHJFFHO_4 | 880445 | 881086 | hlyIII | 0.0 | Hemolysin III | Exotoxin | 99.53 | 100.00 | NP_390062 |
| BCHJFFHO_6 | 930947 | 931732 | dhbA | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.49 | 100.00 | NP_391080 |
| BCHJFFHO_6 | 934630 | 935568 | dhbB | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.47 | 100.00 | NP_391077 |
| BCHJFFHO_6 | 935588 | 942724 | dhbF | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.31 | 100.00 | NP_391076 |
| BCHJFFHO_6 | 510304 | 511446 | capA | 0.0 | Capsule | Immune modulation | 99.21 | 100.00 | NP_391469 |
| BCHJFFHO_6 | 509836 | 510285 | capC | 0.0 | Capsule | Immune modulation | 99.11 | 100.00 | NP_391470 |
| BCHJFFHO_6 | 931758 | 932954 | dhbC | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.00 | 100.00 | NP_391079 |
| BCHJFFHO_4 | 1033970 | 1035733 | dep/capD | 0.0 | Capsule | Immune modulation | 98.92 | 100.00 | NP_389723 |
| BCHJFFHO_6 | 932983 | 934602 | dhbE | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.89 | 100.00 | NP_391078 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| BCHJFFHO_3 | 31799 | 32935 | tufA | 0.0 | elongation factor Tu | 84.96 | 95.69 | AHM69299 |
| BCHJFFHO_1 | 4635 | 5504 | pdxS | 1.14e-151 | encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS | 83.79 | 98.31 | ACE61268 |
| BCHJFFHO_2 | 738969 | 739745 | CodY | 8.46e-125 | nutrient-responsive regulator | 81.08 | 100.00 | ACJ80679 |
| BCHJFFHO_6 | 663774 | 663205 | ClpP | 6.25e-99 | part of proteolytic complex | 81.05 | 97.44 | BAB94595 |
| BCHJFFHO_2 | 276508 | 276900 | spxA1 | 6.65e-70 | redox-responsive transcription factor | 83.97 | 100.00 | AAF21893 |
| BCHJFFHO_2 | 276508 | 276900 | spx | 3.97e-68 | essential gene which is a thiol/oxidative stress sensor | 80.15 | 100.00 | BAB57159 |
| BCHJFFHO_5 | 34626 | 34384 | SpoVG | 7.51e-39 | RNA-binding protein | 81.48 | 79.41 | AAT02994 |
| BCHJFFHO_7 | 77862 | 77665 | CspA | 1.87e-31 | cold shock protein | 90.91 | 100.00 | CAA62903 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| BCHJFFHO_1 | 0.0 | 1.0 | 0.0 |
| BCHJFFHO_2 | 0.0 | 1.0 | 0.0 |
| BCHJFFHO_3 | 0.0 | 1.0 | 0.0 |
| BCHJFFHO_4 | 0.0 | 1.0 | 0.0 |
| BCHJFFHO_5 | 0.0 | 1.0 | 0.0 |
| BCHJFFHO_6 | 0.0 | 1.0 | 0.0 |
| BCHJFFHO_7 | 0.0 | 1.0 | 0.0 |
| BCHJFFHO_8 | 0.0 | 1.0 | 0.0 |
| BCHJFFHO_9 | 1.0 | 0.0 | 1.0 |
| BCHJFFHO_11 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| BCHJFFHO_2 | 370671 | 397595 | False | Myoviridae | VOG4602, VOG9667, VOG3490, VOG0189, VOG4861, VOG5447, VOG0796, VOG10227, VOG4773, VOG4685, VOG0749, VOG3890, VOG3891, VOG3892, VOG6163, VOG4865, VOG4885, VOG3894, VOG4550, VOG4691, VOG4862, VOG7718, VOG4705 |
| BCHJFFHO_4 | 474010 | 503615 | False | Myoviridae | VOG4600, VOG1309, VOG11060, VOG0189, VOG4693, VOG4673, VOG0796, VOG10227, VOG4773, VOG4688, VOG4685, VOG0749, VOG4589, VOG4870, VOG10055, VOG3890, VOG3891, VOG3892, VOG6163, VOG4865, VOG4885, VOG3894, VOG4550, VOG4691, VOG4862, VOG7718 |
| BCHJFFHO_9 | 11005 | 24169 | False | Unknown | VOG4549, VOG2725, VOG3531, VOG3851, VOG2352, VOG4714, VOG4561, VOG1198, VOG0397, VOG2352, VOG11080 |
| BCHJFFHO_9 | 43683 | 67332 | False | Siphoviridae | VOG3616, VOG1024, VOG10944, VOG9244, VOG4599, VOG4605, VOG6163, VOG5852, VOG4586, VOG0704, VOG0539, VOG4589, VOG0204, VOG4553, VOG4568, VOG4556, VOG4544, VOG4581, VOG0221 |
Phigaro v2.4.0 (Database: Jan. 2024 release)